Trang Huyen Lai

Gyeongsang National University | GNU · Graduate School of Medicine

Autophagy plays a critical role in maintaining cell homeostasis in response to various stressors through protein conjugation and activation of lysosome-dependent degradation. MAP1LC3B/LC3B (microtubule associated protein 1 light chain 3 beta) is conjugated with phosphatidylethanolamine (PE) in the membranes and regulates initiation of autophagy thr...

Raf kinase inhibitor protein (RKIP) plays a critical role in many signaling pathways as a multi-functional adapter protein. In particular, the loss of RKIP’s function in certain types of cancer cells results in epithelial to mesenchymal transition (EMT) and the promotion of cancer metastasis. In addition, RKIP inhibits autophagy by modulating LC3-l...

Oxidative damage in neurons including glutamate excitotoxicity has been linked to increasing numbers of neuropathological conditions. Under these conditions, cells trigger several different cellular responses such as autophagy, apoptosis, necrosis and senescence. However, the connection between these responses is not well understood. In this study,...

Background The co-localization analysis of fluorescence microscopy images is a widely used technique in biological research. It is often used to determine the co-distribution of two proteins inside the cell, suggesting that these two proteins could be functionally or physically associated. The limiting step in conducting microscopy image analysis i...

Background Autophagy is involved in the development and differentiation of many cell types. It is essential for the pre-adipocytes to respond to the differentiation stimuli and may contribute to reorganizing the intracellulum to adapt the morphological and metabolic demands. Although AMPK, an energy sensor, has been associated with au-tophagy in se...

Autophagy is the cell self-eating mechanism to maintain cell homeostasis by removing damaged intracellular proteins or organelles. It has also been implicated in the development and differentiation of various cell types including the adipocyte. Several links between adipogenic transcription factors and key autophagy genes has been suggested. In thi...

One million females are diagnosed worldwide every year with breast cancer, and the mortality rate of these patients remains high. Several treatments, including surgery, are available for breast cancer. β‑Lapachone (β‑Lap), a natural quinone compound, has been developed for cancer treatment due to its strong cytotoxic effect through its action on NA...

Background: The co-localization analysis of fluorescence microscopy images is a widely used technique in biological research. It is often used to determine the co-distribution of two proteins inside the cell, suggesting that these two proteins could be functionally or physically associated. The limiting step in conducting microscopy image analysis...

The process of autophagy can be induced by extra-cellular and intracellular stimuli. Several studies suggested a role of oxidative stressors in relying the signals. The list of the interactions between the two pathways is growing. In this study, we identified the gene products of autophagy and oxidative stress using the corresponding gene ontology...

Autophagy, an intracellular degradation process, is essential for maintaining cell homeostasis by removing damaged organelles and proteins under various conditions of stress. In cancer, autophagy has conflicting functions. It plays a key role in protecting against cancerous transformation by maintaining genomic stability against genotoxic component...

Raf kinase inhibitor protein (RKIP) functions as an anti-cancer protein in certain types of cancer cells, so loss of function results in the promotion of cancer progression. RKIP also inhibits autophagy by modulating LC3-lipidation and mTORC1. Other ways of RKIP-dependent inhibition of autophagy specifically during cancer progression are still unde...

Objectives: microRNAs regulate expression of target genes by specifically binding to their transcripts, subsequently leading to translational inhibition or mRNA degradation. Gene regulation by microRNAs has been implicated in a wide range of physiological and pathological conditions. Here, we leverage the use of public-access data and the availabl...

Background Raf kinase inhibitor protein (RKIP) plays a critical role in many signaling pathways as a multi-functional adapter protein. In particular, the loss of RKIP's function in certain types of cancer cells results in ep-ithelial to mesenchymal transition (EMT) and the promotion of cancer metastasis. Also, RKIP inhibits autophagy by modulating...

Autophagy is involved in the development and differentiation of many cell types. It is essential for the pre-adipocytes to respond to the differentiation stimuli and may contribute to reorganizing the intracellulum to adapt the morphological and metabolic demands. Although AMPK, an energy sensor, has been associated with autophagy in several cellul...

Autophagy contributes to reorganizing intracellular components and forming fat droplets during the adipocyte differentiation. Here, we systematically describe the role of autophagy-related genes and gene sets during the differentiation of adipocytes. We used a public dataset from the European Nucleotide Archive from an RNA-seq experiment in which 3...

Background Autophagy contributes to reorganizing intracellular components and forming fat droplets during the adipocyte differentiation. Here, we systematically describe the role of autophagy-related genes and gene sets during the differentiation of adipocytes. Methods We used a public dataset from the European Nucleotide Archive from an RNA-seq ex...

Background Autophagy contributes to reorganizing intracellular components and forming fat droplets during the adipocyte differentiation. Here, we systematically describe the role of autophagy-related genes and gene sets during the differentiation of adipocytes. Methods We used a public dataset from the European Nucleotide Archive from an RNA-seq ex...

Autophagy contributes to reorganizing intracellular components and forming fat droplets during the adipocyte differentiation. Here, we used public RNA-seq data to characterize the role of autophagy at different developmental stages of MDI-induced 3T3-L1 pre-adipocytes. Raw data of 4 different time points were obtained from the Short Read Archive. S...

Phosphatidylethanolamine binding protein 1 (PEBP1) is originally isolated as a Raf kinase inhibitory protein (RKIP). Recently, we reported that PEBP1 negatively regulates starvation-induced autophagy. It specifically binds to LC3 via its own LIR motif (W55DGL58) and prevents LC3 lipidation with phosphatidylethanolamine. Autophagy has also been impl...