Trang Thi Phuong Phan

Trang Thi Phuong Phan
Ho Chi Minh City University of Science | HCMUS · Laboratory of Molecular Biotechnology

PhD

About

41
Publications
11,278
Reads
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588
Citations
Additional affiliations
June 2004 - July 2007
Universität Bayreuth
Description
  • PhD
June 2004 - March 2016
Ho Chi Minh City University of Science
Position
  • false
Education
July 2007 - May 2011
Max-Planck, Dortmund
Field of study
  • Biochemistry

Publications

Publications (41)
Article
Full-text available
The influence of fusion tags to produce recombinant proteins in the cytoplasm of Bacillus subtilis is not well-studied as in E. coli. This study aimed to investigate the influence of His-tags with different codons on the protein production levels of the high expression gene (gfp+) and low expression gene (egfp) in the cytoplasm of B. subtilis cells...
Article
Full-text available
Inducer-free integrative vectors are often used to create B. subtilis strains for industrial purposes, but employing strong promoters to produce high levels of recombinant proteins in B. subtilis results in high leaky expression that can hamper cloning in Escherichia coli. To overcome the problem, we used strong IPTG-inducible Pgrac promoters harbo...
Article
Full-text available
Expression and secretion of recombinant proteins in the endotoxin-free bacterium, Bacillus subtilis, has been thoroughly studied, but overexpression in the cytoplasm has been limited to only a few proteins. Here, we used the robust IPTG-inducible promoter, Pgrac212, to overexpress human rhinovirus 3C protease (HRV3C) in the cytoplasm of B. subtilis...
Article
Introduction: In basic research, it is essential to use an inducible promoter which can be controlled to express a small amount of protein for studying their roles in the cell. Pspac, a well-known weak promoter for Bacillus subtilis, uses isopropyl β-D-1-thiogalactopyranoside (IPTG) as an inducer. However, plasmids carrying this promoter such as pH...
Article
The aim of this study is the selection of some folk medications which can inhibit the Streptococcus mutans causing cavities. The authors isolated 139 strains from 153 dentin caries collected at the National Hospital of Odonto- Stomatology in HCM City. Based on the characteristic colonies on the MSB selective medium (Mitis Salivarius Agar supplement...
Article
The aim of this study is to select some Bacillus isolates which are capable of yielding several beneficial enzymes and antagonism to Vibrio parahaemolyticus causing the EMS shrimp disease. In this study, we isolated and screened total of 54 Bacillus isolates from 30 mud, water and shrimp samples at shrimp ponds in Soc Trang province. Among these, 1...
Article
Full-text available
An approach for dertemining the synergistic activity of cellulosomal catalytic units in culture media is among essential steps in the research of artificial cellulosomes. Endoglucanase A (CelA) and exoglucanase S (CelS) are two abundant cellulosomal cellulases secreted by anaerobic thermophilic bacterium Clostridium thermocellum and mostly responsi...
Article
Early mortality syndrome (EMS) caused by pathogenic Vibrio parahaemolyticus is one of the most major factors affecting the development of aquaculture. Using the antagonism of probiotics against pathogens is an alternative strategy to antibiotics and has lots of potential to control pathogenic bacteria. In this study, we isolated and screened total...
Article
Full-text available
Background Besides Escherichia coli, Bacillus subtilis is an important bacterial species for the production of recombinant proteins. Recombinant genes are inserted into shuttle expression vectors which replicate in both E. coli and in B. subtilis. The ligation products are first transformed into E. coli cells, analyzed for correct insertions, and t...
Chapter
Bacillus subtilis (B. subtilis) is a potential and attractive host for the production of recombinant proteins. Different expression systems for B. subtilis have been developed recently, and various target proteins have been recombinantly synthesized and purified using this host. In this chapter, we introduce a generic protocol to express a recombin...
Article
Full-text available
p24 protein is a component of the HIV particle capsid. It plays an essential role in HIV to infect into the host cell and in the cycle life of virus. Therefore, this protein can be used in the orientative study “to create and produce HIV’s vaccine”. This study created the new Bacillus subtilis strain which expressed p24 protein. B. subtilis a safet...
Article
Listeriolysin O (LLO) is an important extracellular toxin of Listeria monocytogenes which degrades the phospholipid membranes of the host cells’ phagosomes at low pH during the intracellular survival. In contrast to the natural form, the mutant LLO carrying two replacements of amino acid E247M and D320K possesses stable activity at pH 7.4. In this...
Article
Full-text available
Conventional methods for purification of the secreted recombinant proteins at large-volume are uneconomic and inconvenient. CBM3a, a module from cellulosome’s scaffoldin of Clostridium thermocellum, directs the binding of the cellulase complex on the cheap cellulose substrate. Most of previous studies on CBM3a fused with cellulases as a purificatio...
Article
The receptor tyrosine kinase EGFR is regulated by complex conformational changes and this conformational control is disturbed in certain types of cancer. Many ligands are known to bind EGFR in its active conformation, preventing ATP from binding. Alternatively, only a few ligands are known to stabilize EGFR in its inactive conformation thus providi...
Article
Full-text available
This study is to survey the prevalence and antibiotic resistance pattern of Salmonella spp. isolated from the farms at Dak Lak province, Viet Nam. 139 farms including 5 civet farms, 14 pig farms and 120 duck farms were sampled and analyzed. The results showed that many samples collected from 120 duck farms,14 pig farms and 5 civet farms were positi...
Article
Full-text available
Microdeletion on the Y chromosome is one of the causes that makes men infertile, accounting for 2-10 % of all infertility cases, and occurs frequently at 3 regions of the Ychromosome long arm namely AZFa, AZFb and AZFc (azoospermia factor). Currently, the diagnosis of microdeletion on the Y chromosome is almost mandatory in institutes and centers f...
Article
Bacillus subtilis has been developed as an attractive expression host because of many advantages. For examples, it is nonpathogenic and allows secretion of functional extracellular proteins directly into the culture medium; about 60 % of industrial enzymes available produced by Bacillus species. To use B. subtilis strain for research and as host st...
Article
Full-text available
Gfp gene coding for protein GFP (green fluorescent protein) from the jellyfish Aequorea victoria is the most popular indicator protein, due to (i) easy to recognize the expression through the fluorescent ability, (ii) without substrate or cofactor, (iii) less impact on the fusion protein target form. A lot of modifications of GFP have been studied...
Article
Full-text available
In general, fusion of recombinant genes to strong inducible promoters allowing intracellular expression in Bacillus subtilis is a two-step process. The ligation products are transformed into Escherichia coli, followed by identification of the correct plasmid, and this plasmid is subsequently transformed into B. subtilis. This raises the problem tha...
Article
pHT253 and pHT254 belong to the plasmid system carrying Pgrac100, a strong promoter, which were commercialized in the global market in 2012 have pushed forward the use of Bacillus subtilis as host for the production of recombinant proteins. Along with strong promoter Pgrac100, these two plasmids were added His-tag to before and after MCS (Multi Clo...
Article
Full-text available
Endoglucanase A (CelA) is a component of thermostable complex - cellulosome which is produced by anaerobic bacterium Clostridium thermocellum. In this complex, CelA has the highest catalytic activity and it can cleave randomly β-1,4 glucosidic linkage of cellulose to smaller oligosaccharides. Bacillus subtilis a Gram positive and aerobic fast growt...
Article
Bacillus subtilis has been a model for research on Gram-positive bacteria for more than four decades. It is a non-pathogenic bacterium and is conferred the GRAS (Generally Recognized As Safe) status by FDA. B. subtilis has also been widely used in the production of industrial proteins because of its high secretion capacity into the culture medium....
Article
In addition to the catalytically active kinase domain, most kinases feature regulatory domains that govern their activity. Modulating and interfering with these inter-domain interactions presents a major opportunity for understanding biological systems and developing novel therapeutics. Therefore, small molecule inhibitors that target these interac...
Article
Discoidin domain-containing receptors (DDRs) exhibit a unique mechanism of action among the receptor tyrosine kinases (RTKs) since their catalytic activity is induced by extracellular collagen binding. Moreover, they are essential components in the assimilation of extracellular signals and are frequently associated with cancer progression. Recently...
Article
Different mRNA stabilizing elements including the 3'-stem-loop, the ribosome binding sites (RBS), the 5'-stem-loop and the spacer region between the RBS and the 5'-stem-loop were analysed in detail to increase mRNA stability resulting in enhanced expression of heterologous proteins. In addition, in combination with mRNA stabilizing elements, we pro...
Conference Paper
Full-text available
In recent decades, Bacillus subtilis (B. subtilis for short) becomes a good alternative strain to the traditional Escherichia coli host bacterium in recombinant protein production. For this reason, several research groups focus on improving yield of heterologous protein production in B. subtilis. Investigating optimal culture medium for B. subtilis...
Article
LTB is the B subunit of heat labile toxins (LT) in Escherichia coli ETEC. This subunit is non-toxic but has a high immune response. Therefore, LTB is considered a suitable antigen for partial vaccine against the diarrhea caused by E. coli ETEC. The most important component of partial vaccine is antigen protein. Nowadays, with the advancement of rec...
Article
Nuclear receptor binding to coactivator proteins is an obligate first step in the regulation of gene transcription. Nuclear receptors preferentially bind to an LXXLL peptide motif which is highly conserved throughout the 300 or so natural coactivator proteins. This knowledge has shaped current understanding of this fundamental protein-protein inter...
Article
The Estrogen Receptors ERα and ERβ bind cofactor proteins via short LXXLL motifs. The exact regulation and selectivity of these interactions remains an open question and the role of post-translational modifications (PTMs) is virtually unexplored. Here, we designed an X(7)-LXXLL-X(7) T7 phage display library and screened this against four ER protein...
Article
Transcription efficiency of inducible promoters remains a bottleneck in recombinant protein production in Bacillus subtilis cells. Here, we present experimental data how to generate strong IPTG-inducible promoters by optimization of nucleotides at the conserved regions of the groESL promoter including the UP element, the -35, -15, -10 and the +1 re...
Article
Full-text available
Bacillus subtilis codes for two putative sortases, YhcS and YwpE, and two surface proteins, YhcR and YfkN, harboring sorting motifs supposed to be recognized by the putative sortase(s). However, there is no experimental evidence to show a direct link between these sortases and sorting sequences. To study the role of these two putative sortases on d...
Article
Miniprotein phage display screening yields structured peptides with high affinity for the Estrogen Receptor (ER). Hits from apamin phage libraries feature a LXXLL motif specifically placed on the predefined miniprotein helical segment. The apamin scaffold also allows optimization of flanking amino acids to ensure an optimal ER binding affinity.
Article
Using published plasmid vectors containing the bgaB gene encoding a heat-stable beta-galactosidase, we have been unable to fuse strong promoters to this reporter gene. In addition, we could not analyze the promoter strength by a plate assay. Therefore, we constructed an Escherichia coli-Bacillus subtilis shuttle vector to allow the cloning of stron...
Article
The interaction of estrogen receptor alpha (ERalpha) with the consensus LXXLL motifs of transcriptional coactivators provides an entry for functional ERalpha inhibition. Here, synthetic cell-permeable LXXLL peptide probes are brought forward that allow evaluation of the interaction of specific recognition motifs with ERalpha in the context of the c...
Article
About 2% of the Bacillus subtilis genes are subject to regulation by riboswitch-controlled mechanisms. One of them is the L-lysine-dependent lysC gene which is turned on when the L-lysine concentration within the cytoplasm is low. In the presence of a high L-lysine concentration, only a 0.27-kb transcript is synthesized representing the riboswitch...
Article
Full-text available
We describe the construction of six novel plasmid-based IPTG-inducible expression vectors for Bacillus subtilis and related species. While one vector allows intracellular production of recombinant proteins, the second provides a strong secretion signal. The third vector allows addition of the c-Myc epitope tag, and the remaining three vectors provi...
Article
Riboswitches are binding domains for metabolites located within the 5' untranslated regions (UTR) of mRNA molecules. With two exceptions, binding of the metabolite prevents expression of the downstream gene(s). In one of these exceptions, binding of the metabolite glycine triggers expression of the downstream genes in Bacillus subtilis involved in...
Article
Two plasmid-based expression vectors have been constructed where one allows intracellular production of recombinant proteins while the second directs the proteins into the culture medium. Both vectors use the strong promoter preceding the groESL operon (codes for the essential heat shock proteins GroES and GroEL) of Bacillus subtilis fused to the l...

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Projects (3)
Project
Mainly focus on Gram-positive bacteria such as Bacillus and Lactobacillus species and Gram-negative bacteria E. coli.