Trang Huyen Lai

Trang Huyen Lai
Gyeongsang National University | GNU · Department of Convergence Medical Science

Doctor of Philosophy
Cell-based screening system and regulation of Oncology genes in Cancer, Diabetes, Autophagy

About

38
Publications
6,642
Reads
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226
Citations
Citations since 2017
37 Research Items
226 Citations
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Introduction
Trang Huyen Lai is a postdoctoral researcher at the department of Convergence Medical Science, School of Medicine, Gyeongsang National University, South Korea. Using molecular technology and experimental assays, Trang focuses on the processes and regulatory pathways of genes implicated in autophagy and cancer. Her current research investigates the role of Raf kinase inhibitory protein (RKIP) in breast cancer metastasis and its regulators.
Additional affiliations
March 2018 - present
Gyeongsang National University
Position
  • PhD Student
Education
March 2016 - March 2018
Gyeongsang National University
Field of study
  • Department of Convergence Medical Science

Publications

Publications (38)
Article
Autophagy plays a critical role in maintaining cell homeostasis in response to various stressors through protein conjugation and activation of lysosome-dependent degradation. MAP1LC3B/LC3B (microtubule associated protein 1 light chain 3 beta) is conjugated with phosphatidylethanolamine (PE) in the membranes and regulates initiation of autophagy thr...
Article
Full-text available
Raf kinase inhibitory protein (RKIP), also known as a phosphatidylethanolamine-binding protein 1 (PEBP1), functions as a tumor suppressor and regulates several signaling pathways, including ERK and NF-κB. RKIP is severely downregulated in human malignant cancers, indicating a functional association with cancer metastasis and poor prognosis. The tra...
Article
Full-text available
Autophagy is a highly conserved metabolic process involved in the degradation of intracellular components including proteins and organelles. Consequently, it plays a critical role in recycling metabolic energy for the maintenance of cellular homeostasis in response to various stressors. In cancer, autophagy either suppresses or promotes cancer prog...
Article
Full-text available
Differentiating 3T3-L1 pre-adipocytes are a mixture of non-identical culture cells. It is vital to identify the cell types that respond to the induction stimulus to understand the pre-adipocyte potential and the mature adipocyte behavior. To test this hypothesis, we deconvoluted the gene expression profiles of the cell culture of MDI-induced 3T3-L1...
Article
Full-text available
Drug screening strategies focus on quantifying the phenotypic effects of different compounds on biological systems. High-throughput technologies have the potential to understand further the mechanisms by which these drugs produce the desired outcome. Reverse causal reasoning integrates existing biological knowledge and measurements of gene and prot...
Article
Full-text available
Metastasis is associated with poor prognosis and is the major cause of death in cancer patients. The epithelial to mesenchymal transition (EMT) is essential for cancer cells to acquire a highly migratory phenotype. Metabolic reprogramming is required to meet the energy demands during this process. Recent studies have indicated that autophagy is inv...
Article
Full-text available
Intervertebral disc degeneration (IVDD) is a prevalent cause of low back pain. IVDD is characterized by abnormal expression of extracellular matrix components such as collagen and aggrecan. In addition, it results in dysfunctional growth, senescence, and death of intervertebral cells. The biological pathways involved in the development and progress...
Poster
Full-text available
Breast cancer is the most prevalent type of cancer among women, and the vast majority of deaths are due to metastasis. Effective cancer therapies require an understanding of the underlying mechanisms and regulatory pathways of genes involved in metastasis. We applied text mining and a manual literature search to extract known interactions between s...
Presentation
Screening for potential cancer therapies using existing large datasets of drug perturbations requires expertise and resources not available to all. This is often a barrier for lab scientists to tap into these valuable resources. To address these issues, one can take advantage of prior knowledge especially those coded in standard formats such as cau...
Article
Full-text available
Backgound Autophagy controls levels of cellular components during normal and stress conditions; thus, it is a pivotal process for the maintenance of cell homeostasis. In cancer, autophagy protects cells from cancerous transformations that can result from genomic instability induced by reactive oxygen species or other damaged components, but it can...
Article
Full-text available
We previously showed that some adipogenic transcription factors such as CEBPB and PPARG directly and indirectly regulate autophagy gene expression in adipogenesis. The order and effect of these events are undetermined. In this study, we modeled the gene expression, DNA-binding of transcriptional regulators, and histone modifications during adipocyt...
Article
Aims: The development of osteoarthritis (OA), the most common form of arthritis, is commonly associated with oxidative stress. Indeed, the lack of antioxidant responses largely increases OA incidence. OA is a leading cause of disability in the elderly, which reduces the quality of life and places high socioeconomic burdens on them. Several polyphe...
Poster
Full-text available
Approach. We modeled the gene expression, DNA-binding of transcriptional regulators, and histone modifications during adipocyte differentiation [1, preprint available]. We evaluated the effect of the binding of the regulators on gene expression in terms of direction and magnitude. Then, we identified the overlap of the transcription factors and co-...
Poster
Full-text available
Raf kinase inhibitory protein (RKIP), also known as a phosphatidylethanolamine-binding protein 1 (PEBP1), functions as a tumor suppressor and regulates several signaling pathways, in- cluding ERK and NF-κB. RKIP is severely downregulated in human malignant cancers, indicating a functional association with cancer metastasis and poor prognosis. The t...
Preprint
Full-text available
Differentiating 3T3-L1 pre-adipocytes are a mixture of non-identical culture cells. It is vital to identify the cell types that respond to the induction stimulus to understand the pre-adipocyte potential and the mature adipocyte behavior. To test this hypothesis, we deconvoluted the gene expression profiles of the cell culture of MDI-induced 3T3-L1...
Preprint
Full-text available
We previously showed that some adipogenic transcription factors such as CEBPB and PPARG directly and indirectly regulate autophagy gene expression in adipogenesis. The order and the effect of these events are undetermined. In this study, we modeled the gene expression, DNA-binding of transcriptional regulators, and histone modifications during adip...
Poster
Full-text available
Background The binding of transcription factors to the gene regulatory regions induces or represses its expression. Co-factors and/or other DNA-binding proteins share the binding sites with transcription factors. These binding events do not always lead to a functional interaction. Results We developed a method to predict the combined functions of t...
Article
Full-text available
Autophagy is the cell self-eating mechanism to maintain cell homeostasis by removing damaged intracellular proteins or organelles. It has also been implicated in the development and differentiation of various cell types including the adipocyte. Several links between adipogenic transcription factors and key autophagy genes has been suggested. In thi...
Article
Full-text available
One million females are diagnosed worldwide every year with breast cancer, and the mortality rate of these patients remains high. Several treatments, including surgery, are available for breast cancer. β‑Lapachone (β‑Lap), a natural quinone compound, has been developed for cancer treatment due to its strong cytotoxic effect through its action on NA...
Article
Full-text available
Background: The co-localization analysis of fluorescence microscopy images is a widely used technique in biological research. It is often used to determine the co-distribution of two proteins inside the cell, suggesting that these two proteins could be functionally or physically associated. The limiting step in conducting microscopy image analysis...
Poster
Full-text available
The process of autophagy can be induced by extra-cellular and intracellular stimuli. Several studies suggested a role of oxidative stressors in relying the signals. The list of the interactions between the two pathways is growing. In this study, we identified the gene products of autophagy and oxidative stress using the corresponding gene ontology...
Preprint
Full-text available
Background The co-localization analysis of fluorescence microscopy images is a widely used technique in biological research. It is often used to determine the co-distribution of two proteins inside the cell, suggesting that these two proteins could be functionally or physically associated. The limiting step in conducting microscopy image analysis i...
Preprint
Full-text available
Background The co-localization analysis of fluorescence microscopy images is a widely used technique in biological research. It is often used to determine the co-distribution of two proteins inside the cell, suggesting that these two proteins could be functionally or physically associated. The limiting step in conducting microscopy image analysis i...
Article
Full-text available
Autophagy, an intracellular degradation process, is essential for maintaining cell homeostasis by removing damaged organelles and proteins under various conditions of stress. In cancer, autophagy has conflicting functions. It plays a key role in protecting against cancerous transformation by maintaining genomic stability against genotoxic component...
Article
Oxidative damage in neurons including glutamate excitotoxicity has been linked to increasing numbers of neuropathological conditions. Under these conditions, cells trigger several different cellular responses such as autophagy, apoptosis, necrosis and senescence. However, the connection between these responses is not well understood. In this study,...
Poster
Full-text available
Raf kinase inhibitor protein (RKIP) functions as an anti-cancer protein in certain types of cancer cells, so loss of function results in the promotion of cancer progression. RKIP also inhibits autophagy by modulating LC3-lipidation and mTORC1. Other ways of RKIP-dependent inhibition of autophagy specifically during cancer progression are still unde...
Article
Full-text available
Objectives: microRNAs regulate expression of target genes by specifically binding to their transcripts, subsequently leading to translational inhibition or mRNA degradation. Gene regulation by microRNAs has been implicated in a wide range of physiological and pathological conditions. Here, we leverage the use of public-access data and the availabl...
Poster
Full-text available
Background Raf kinase inhibitor protein (RKIP) plays a critical role in many signaling pathways as a multi-functional adapter protein. In particular, the loss of RKIP's function in certain types of cancer cells results in ep-ithelial to mesenchymal transition (EMT) and the promotion of cancer metastasis. Also, RKIP inhibits autophagy by modulating...
Article
Full-text available
Raf kinase inhibitor protein (RKIP) plays a critical role in many signaling pathways as a multi-functional adapter protein. In particular, the loss of RKIP’s function in certain types of cancer cells results in epithelial to mesenchymal transition (EMT) and the promotion of cancer metastasis. In addition, RKIP inhibits autophagy by modulating LC3-l...
Article
Full-text available
Autophagy is involved in the development and differentiation of many cell types. It is essential for the pre-adipocytes to respond to the differentiation stimuli and may contribute to reorganizing the intracellulum to adapt the morphological and metabolic demands. Although AMPK, an energy sensor, has been associated with autophagy in several cellul...
Poster
Full-text available
Background Autophagy is involved in the development and differentiation of many cell types. It is essential for the pre-adipocytes to respond to the differentiation stimuli and may contribute to reorganizing the intracellulum to adapt the morphological and metabolic demands. Although AMPK, an energy sensor, has been associated with au-tophagy in se...
Article
Full-text available
Autophagy contributes to reorganizing intracellular components and forming fat droplets during the adipocyte differentiation. Here, we systematically describe the role of autophagy-related genes and gene sets during the differentiation of adipocytes. We used a public dataset from the European Nucleotide Archive from an RNA-seq experiment in which 3...
Poster
Full-text available
Background Autophagy contributes to reorganizing intracellular components and forming fat droplets during the adipocyte differentiation. Here, we systematically describe the role of autophagy-related genes and gene sets during the differentiation of adipocytes. Methods We used a public dataset from the European Nucleotide Archive from an RNA-seq ex...
Poster
Full-text available
Background Autophagy contributes to reorganizing intracellular components and forming fat droplets during the adipocyte differentiation. Here, we systematically describe the role of autophagy-related genes and gene sets during the differentiation of adipocytes. Methods We used a public dataset from the European Nucleotide Archive from an RNA-seq ex...
Poster
Full-text available
Autophagy contributes to reorganizing intracellular components and forming fat droplets during the adipocyte differentiation. Here, we used public RNA-seq data to characterize the role of autophagy at different developmental stages of MDI-induced 3T3-L1 pre-adipocytes. Raw data of 4 different time points were obtained from the Short Read Archive. S...
Poster
Full-text available
Phosphatidylethanolamine binding protein 1 (PEBP1) is originally isolated as a Raf kinase inhibitory protein (RKIP). Recently, we reported that PEBP1 negatively regulates starvation-induced autophagy. It specifically binds to LC3 via its own LIR motif (W55DGL58) and prevents LC3 lipidation with phosphatidylethanolamine. Autophagy has also been impl...

Questions

Question (1)
Question
Hi everyone,
I am currently working on the differentiation of 3T3-L1 cells in Lipogenesis. Just in the starting point, I noticed that my cell morphology is not handsome compared with the other publications. My cells are fat in the middle and start to extend at the edges into the spindles. Also, these spindle-like cells overlap each other like the spider nets.
Here, I attached some of my cell pictures at the low and high confluence. Could someone please take a look and tell me whether if my cells are healthy? Should I continue my experiments with these cells?
Thank you.

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Projects

Projects (6)
Project
Deciphering the regulatory circuits driving RKIP loss in different cancer cell models.
Project
Studying causal biological networks and their perturbation using existing biological knowledge and data.
Project
Reveal the regulation of MTDH and its implication in starvation-induced autophagy process.