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Publications (134)
Background: Hand, Foot, and Mouth disease is an acute infectious disease caused by a group of enteroviruses, including Coxsackievirus A16 and Enterovirus 71. EV-A71-causing disease can give rise to severe complications in children, leading to meningitis, encephalitis, and even death. A potential approach to prevent the virus spread is inhibiting th...
Acute hepatopancreatic necrosis disease (AHPND) is currently the most affected disease of shrimp farms worlwide As rapid and specific detection methods are essential to improve disease management, a lateral flow immunoassay (LFIA) was designed for the detection of PirAvp and PirBvp toxins in shrimp. In this study, polyclonal antibody based lateral...
In this study, a series of secondary metabolites from Ganoderma sp. were screened against Staphylococcus aureus protein targets, including as phosphotransacetylase, clumping factor A, and dihydrofolate reductase, using molecular docking simulations. The chemicals that showed the strongest binding energy with the targeted proteins were ganodermanont...
Fibroblast growth factor 2 (FGF-2) is a protein that plays an important role in the skin wound healing process. However, the biomedical application of this protein has some limitations due to its instability. To address this problem, this study developed carboxymethyl cellulose (CMC) nanoparticles (NPs) as a nano-carrier for FGF-2 encapsulation and...
Selenium nanoparticles (SeNPs) attract more and more interest due to good bioavailability and low toxicity, accompanied by various bioactivities consisting of antimicrobial, antioxidant, and anticancer activity. The SeNPs could be generated by physical, chemical, or biological methods, and their potential depends on the particle diameter, homogenei...
The abnormal expression in the T-type calcium channels is involved in various cancer types, thus inhibiting T-type calcium channels is one of approaches in cancer treatment. The fact that KTt-45 acted as a T-type calcium channel inhibitor as well as a pain-relief agent prompts us to address if KTt-45 plays any role against cancer cells. The results...
A recombinant Pichia pastoris strain which is genetically modified for highly expressing pediocin PA-1 and secreted into a medium so that the pediocin PA-1 is easily collected and purified from the culture medium. The obtained pediocin PA-1 has specific antimicrobial activities against Listeria monocytogenes ATCC 13932 and several other bacteria st...
Ganoderma multipileum, a wood decay mushroom, was initially discovered and classified in Taiwan through the analysis of its morphology and the internal transcribed spacer (ITS) region of nuclear ribosomal DNA. In this study, we identified a mushroom associated with the dieback of Delonix regia (Boj. ex Hook.) Raf., a woody ornamental street tree in...
The polysaccharides found in Amauroderma subresinosum have been recognized, but their characterization has been limited. In this study, enriched polysaccharide fractions (ASF) were extracted from the mycelia of Amauroderma subresinosum using hot water, sodium hydroxide, and acetone. To determine these ASF structural characteristics, deproteination...
Porcine epidemic diarrhea virus (PEDV) is a highly contagious intestinal virus. However, the current PEDV vaccine, which is produced from classical strain G1, offers low protection against recently emerged strain G2. This study aims to develop a better vaccine strain by propagating the PS6 strain, a G2b subgroup originating from Vietnam, on Vero ce...
Background: Staphylococcus aureus is a nosocomial pathogen responsible for many serious infectious diseases in humans. Finding the anti- S. aureus agents is a time-consuming and costly process. Recently, computational methods have provided a better understanding of the interactions between herbal medicine drug targets to help clinical practitioners...
Background: Lateral flow immunoassay (LFIA) is a well-known method because of its extensive application in many fields, such as medical, food safety, and environmental analysis. LFIA is also under development for pathogen or protein detection, where rapid detection is essential. However, there are few studies on the optimal process for LFIA product...
The distinctive morphology characteristics of microfold cells (M cells) allow the vaccine antigen not only to interact with immune cells directly, but also to effectively stimulate mucosal immune responses via receptors on its apical surface. Human prion protein, a transmembrane receptor for Brucella abortus Hsp60, is highly expressed on the M cell...
The lethal PirAvp and PirBvp toxins from Vibrio parahaemolyticus are responsible for acute hepatopancreatic necrosis disease (VPAHPND) in shrimp. After causing infection in the stomach of shrimp, VPAHPND released PirAvp and PirBvp toxins into the culture medium and lead to massive sloughing of cells. These effects resulted in high mortality rate du...
Astaxanthin (AX) is a natural compound that regulates lipid metabolism in the liver, specifically in reducing hepatosteatosis. To the increment in Non-Alcoholic Fatty Liver Diseases (NAFLD) ratio and its burdens, prevention/treatment should address the world health problem. This study aimed to evaluate the ability of AX and its prolonged effects on...
Ganoderma multipileum was initially discovered in Taiwan as a wood decay fungus on many host plants. However, the detailed taxonomy of this genus has suffered from a lack of in-depth investigation. In this study, the classification of these fungi was described based on their morphology, the phylogeny of three regions (ITS, RPB2, and TEF1 sequences)...
Acute hepatopancreatic necrosis disease (AHPND) is a contagious disease for the shrimp cultivation, thus early detection of disease is an unmet need. This present study documented for the first time a simple lateral flow immunoassay (LFIA) strip using polyclonal antibodies was created for the rapid detection both of PirAvp and PirBvp protein simult...
: F18 plays an important role in helping Enterotoxigenic Escherichia coli (ETEC) binds to specific receptors on small intestinal enterocytes, followed by secreting of toxins causing diarrhea in post-weaning piglets (post-weaning diarrhea, PWD). However, the F18 subunit vaccine is not sufficient to stimulate an immune response that can protect weani...
Members of the Ganodermataceae are used in traditional medicine in Asian
countries. In Vietnamese Traditional Medicine, these mushrooms are used to
treat several diseases since the 15th century. In this study, five new
Ganodermataceae specimens as Ganoderma multiplicatum, Ganoderma
sinense, 2 strains of Ganoderma lingzhi, and Amauroderma subresinos...
Antibiotic resistance is a significant issue posing risks to public and animal health due to the declined treatment efficiency of infectious diseases. The presence of antibiotic resistance components such as antibiotic residues, antibiotic-resistant bacteria, and antibiotic resistance genes has been found in a variety of environments affected by hu...
Recombinant DNA technology has been playing the key role for a long time since its first beginning. DNA ligases have certainly contributed to the development of cloning techniques, as well as molecular study up to now. Despite being a prime cloning tool, DNA ligases still face some shortcomings which lead to their limit of use. Our study provided a...
Water quality for the surface water along the Saigon River in Ho Chi Minh City was assessed for four groups of water samples collected at the agricultural, industrial, residential, and less impacted areas. A variety of parameters indicating water quality including physicochemical parameters, nutrients, heavy metals, and antibiotic residues were mea...
From the PRRSV virulent strain BG8 isolated from a PRRSV-infected pig, using serial passage method in MARC-145 cell line, we have successfully obtained an attenuated strain in 95th passage, named as BG895, with high potential to be a vaccine candidate. In this study, we present the results of the safety and efficacy evaluation of BG895 against PRRS...
The present invention provides novel reagents and a cloning procedure based on homologous recombination for the site-directed cloning of a DNA fragment to a vector at designed site(s). The cloning reagents are made of mixture of extracts from at least two different cell types, preferably a mixture made of extracts from wild-type E. coli and S. cere...
Background
Acute hepatopancreatic necrosis disease (AHPND) is caused by toxin-producing strains of Vibrio parahaemolyticus which contain deadly binary toxins PirA vp and PirB vp encoded in pVA1 plasmid. The polyclonal antibodies against PirB vp protein could be used to develop immunochromatographic test strip for in-field diagnosis of AHPND.
Resul...
Background
Gastric cancer is one of the most leading causes of cancer death worldwide. Therefore, treatment studies have been being conducted, one of which is screening of novel agents from medicinal herbs. Elephantopus mollis Kunth (EM) belonging to Asteraceae family is a perennial herb with several therapeutic properties including anticancer acti...
Background:
The recombinant human granulocyte colony stimulating factor conjugated with polyethylene glycol (PEGylated GCSF) has currently been used as an efficient drug for the treatment of neutropenia caused by chemotherapy due to its long circulating half-life. Previous studies showed that Granulocyte Colony Stimulating Factor (GCSF) could be e...
Antibiotic resistance causes serious health problems associated with an increase in hospitalization time, treatment cost, and mortality. In this study, the contamination levels of antibiotic resistant bacteria were determined in the Saigon River, which is impacted by different anthropogenic activities in Ho Chi Minh City. Five target antibiotics we...
Fibroblast growth factor 2 (FGF-2) is a multifunctional protein that has major roles in wound healing, tissue repair, and regeneration. This therapeutic protein is widely used for burn treatment because it can stimulate cell proliferation and differentiation, angiogenesis, and extracellular matrix remodeling. In this study, we developed a simple me...
Despite of a high abundance of antibiotics, heavy metals, and organic matters detected in the Saigon River in Ho Chi Minh City, the level and spread of antibiotic resistance genes in this river are poorly understood. In this study, total 10 antibiotic resistance genes (ARGs), including genes conferring resistance to aminoglycosides (aac(6)-Ib-cr),...
Porcine reproductive and respiratory syndrome (PRRS) is one of the most dangerous infectious disease of the swine industry, caused by PRRSV, a single-stranded, positive-sense RNA virus. The viral envelope contains 6 structural proteins; among these, GP5 is the highly mutation-sensitive antigenic protein which plays important role in viral infection...
Highly expressed genes [HEG] are genees available in the organism, which carry the preferred codons for the expression system. Identifying HEG helps to find preferred codons and use them in the gene optimization to express target protein. Currently, HEG-DB are the only database to store HEG data of many strains of microorganisms, but the data are s...
Acute Hepatopancreatic Necrosis Disease (AHPND) is a newly emerging shrimp disease with mortality up to 100 percent caused by Vibrio parahaemolyticus which carries a plasmid encoding for two toxins, ToxA and ToxB. In 2013, the Global Aquaculture Alliance (GAA) estimated shrimp farming decline in Asia accounted for 1-billion US dollar lost. Currentl...
Recombinant granulocyte colony–stimulating factor (G-CSF) protein produced in Escherichia coli has been widely used for the treatment of neutropenia induced by chemotherapy for decades. In E. coli cells, G-CSF is usually expressed as inactive inclusion bodies, which requires costly and inefficient denaturation and refolding steps to obtain the prot...
Hand, foot and mouth disease is a common viral infectious disease caused by enteroviruses, including coxsackie A16 (CVA16) and enterovirus 71 (EV71). HFMD can cause severe symptoms in children which can be fatal. Human scavenger receptor class B member 2 (SCARB2) is a cellular receptor for EV71 and CVA16, providing a potential approach for preventi...
Inducer-free integrative vectors are often used to create B. subtilis strains for industrial purposes, but employing strong promoters to produce high levels of recombinant proteins in B. subtilis results in high leaky expression that can hamper cloning in Escherichia coli. To overcome the problem, we used strong IPTG-inducible Pgrac promoters harbo...
In this study, we compared the genetic mutation and virulence of the attenuated PRRSV strains obtained by 95 serial passages in Marc-145 cells with the parental virulent strain (designated as BG81) isolated in Vietnam. Results showed that there were marked changes in virulence: pigs inoculated with BG81 exhibited high fever ( 41◦C), which lasted fo...
Acute Hepatopancreatic Necrosis Disease (AHPND) is a globally serious threat, especially for Vietnam shrimp culture. This disease is caused by toxin-carrying bacteria Vibrio parahaemolyticus which plasmid encodes binary toxins ToxA and ToxB. The disease has a mortality rate by up to 100% after 3–5 days post infection and fast spread among shrimp cu...
Immobilizing antibodies on the nitrocellulose membrane is an important step to increase the sensitivity of the Lateral Flow Test strip for detecting pathogenic antigen. In our research, the fusion protein between nitrocellulose-binding anchor protein 3-Helix - a protein that has a strong affinity to nitrocellulose membrane and protein A - a protein...
MTT assay has been applied widely in stimulation and inhibition tests for adherent cells. However, reading step in regular MTT assays requires medium removal to dissolve formazan by absolute solvents results are usually affected by incomplete formazan dissolution and protein precipitation in diluted solvents. Therefore, most of current MTT assay pr...
Hematopoietic stem cell (HSC) transplantation has become an accepted therapeutic modality for patients with hematopoietic disorders. However, transplant recipients are at high risk of a severe complication known as graft versus host disease (GvHD). To reduce the risk of GvHD, depletion of T cells in donor HSCs prior to transplantation is of vital i...
Ethnopharmacological relevance
Elephantopus mollis Kunth (EM), which belongs to Asteraceae family, has been used as a folk medicine with diverse therapeutic properties. Previous studies reported that crude extracts of this plant could inhibit several cancer cell lines, including breast carcinoma MCF-7, liver carcinoma HepG2, colorectal carcinoma DL...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the confirmed viral pathogen of COVID-19, a pandemic originated from Wuhan, China at the end of 2019. Since then, SARS-CoV-2 has rapidly spread across the globe with over 8 million confirmed cases and more than 430.000 deaths worldwide as of mid-June 2020. Similar to other strains of c...
In recombinant protein production, transferring a wild type gene of one organism into another expression host sometime resulted in a low gene expression due to incompatibility between the gene and the expression system. In that case, the target gene needed to be optimized to be more compatible with the expression system through gene optimization pr...
Background:
The number of oral vaccines is still limited due to many difficulties suffered in the intestinal environment, such as mucosal clearance, vast area, harsh conditions, deteriorative enzymes, impermeability, tolerance, etc. Numerous strategies have focused on directing antigen to the receptors of M cells, which is the main gateway to acqu...
Chronic leg ulcer is one of the diabetic complications causing continuous pain and higher mortality risk. The therapeutic treatment of chronic diabetic wounds can be studied through animal models which simulate the human pathology. Experimental diabetes in mice induced by streptozotocin (STZ) is an easy method with low cost. There have been several...
The order Cypriniformes is the most diverse group of freshwater fishes (about 3500 valid species). To date a few molecular taxonomical studies has been conducted on cypriniform fish in Mekong River Basin. The purpose of this study was to provide genetic data and new perspective to the fish classification system in the Mekong River. Combined data of...
The relationship between oxidative stress and neurodegenerative diseases has been extensively examined, and antioxidants are considered to be a promising approach for decelerating disease progression. Parkinson’s disease (PD) is a common neurodegenerative disorder and affects 1% of the population over 60 years of age. A complex combination of genet...
Catfish species in the Order Siluriformes distribute widely in Mekong River Basin, where they are important economic species for aquaculture and fisheries, providing major food sources for local communities. However, phylogenetic relationships of Siluriformes have remained unresolved at high taxonomic level. In this study, phylogenetic trees of 30...
An approach for dertemining the synergistic activity of cellulosomal catalytic units in culture media is among essential steps in the research of artificial cellulosomes. Endoglucanase A (CelA) and exoglucanase S (CelS) are two abundant cellulosomal cellulases secreted by anaerobic thermophilic bacterium Clostridium thermocellum and mostly responsi...
On behalf of the entire the international conference Innovations in Cancer Research and Regenerative Medicine 2017 (CRRM2017) Organizing Committee, we would like to extend to you a warm welcome to Ho Chi Minh city, Vietnam to join the 3rd international conference of CRRM. We are very glad to announce that this time, CRRM has made some exciting chan...
Background
Besides Escherichia coli, Bacillus subtilis is an important bacterial species for the production of recombinant proteins. Recombinant genes are inserted into shuttle expression vectors which replicate in both E. coli and in B. subtilis. The ligation products are first transformed into E. coli cells, analyzed for correct insertions, and t...
Fibroblast growth factor 2 (FGF-2) is a multi-functional protein involving in wound healing. However, FGF-2 is easily degradable in vivo, which limited its use for wound treatment. In this study, we investigated a drug-delivery model for FGF-2 via incorporation with biodegradable carboxylmethyl chitosan (CMCS). CMCS nanoparticles (NPs) could be syn...
At the present time, non-vascular plants (bryophytes) are less studied, especially in Vietnam. Therefore, this research focuses on studying biological activities of some bryophytes species collected in Bidoup–Nui Ba National Park, such as antioxidative, antibacterial activity, acetylcholinesterase inhibitory activity and quantitative analysis of th...
Melicope pteleifolia (Champ. ex benth.) T.g. Hartley, a folk medicinal plant, is used by ethnic minorities in Bidoup–Nui Ba National Park, Lam Dong Province, Vietnam to treat effectively wound, inflammation and skin ulcer. To scientifically prove the claimed utilization and understand the mechanism of action of the plant, the in vitro and in vivo h...
Inflammation is the response of living tissues to the injury. Prolonged or inappropriate inflammation has been involved with many diseases such as, cancer, diabetes, heart disease… These days, inflammation has been treated by nonsteroidal and steroidal anti-inflammatory drugs, which have a lot of side effects. It opens the need and interest of new...
Conyza canadensis (L.) Cronquist (CCL) has been used by K’Ho minority in Bidoup Nui Ba national park, Lam Dong province, Vietnam as one of wound healing remedies. However, the scientific proof of treatment is still unclear. This study aimed to evaluate this wound healing potential of CCL. CCL power was extracted by ethanol and then partitioned cons...
Chloramphenicol (CAP) is a broad-spectrum antibiotic of high toxicity on human therefore it is being strictly controlled in food. We are interested in the development of a method of effective extraction of CAP in food based on the immunological principle using specific anti-CAP antibody combining with LC/MS/MS for the analysis of the residual CAP i...
Anti-chloramphenicol rabbit antibody was covalently bound to Sepharose CL 4B-CNBr gel at 5, 10 and 15 mg antibody per mL gel and was fed into columns to prepare three respective immunoaffinity chromatography columns for chloramphenicol (CAP) binding. Examination of CAP-binding efficiency of these columns being fed with 0.5, 1 and 10 ng CAP showed t...
Listeriolysin O (LLO) is an important extracellular toxin of Listeria monocytogenes which degrades the phospholipid membranes of the host cells’ phagosomes at low pH during the intracellular survival. In contrast to the natural form, the mutant LLO carrying two replacements of amino acid E247M and D320K possesses stable activity at pH 7.4. In this...
Production of KPC-type carbapenemase is the most common carbapenem resistant mechanism in Klebsiella pneumoniae. The expression level of KPC in these strains is different and is mostly required other mechanisms to reach the higher resistant level such as porin lost or co-expression of extended spectrum β-lactamase (ESBL). To better understand the e...
Conventional methods for purification of the secreted recombinant proteins at large-volume are uneconomic and inconvenient. CBM3a, a module from cellulosome’s scaffoldin of Clostridium thermocellum, directs the binding of the cellulase complex on the cheap cellulose substrate. Most of previous studies on CBM3a fused with cellulases as a purificatio...
In our research, we have presented the controlled synthesis of Fe3O4 nanoparticles (NPs) with a size of about 10 nm coated with SiO2 shells for bio-applications. On this basis, the controlled synthesis of Fe3O4-SiO2 core-shell nanoparticles and their surface functionalization with 3-glycidoxypropyltrimethoxysilane (GPS) and 1,1′-carbonyldiimidazole...
Nocosomial pathogen Klebsiella pneumoniae is a gram - negative bacterium that carries multiple antimicrobial resistant genes. Conjugative method was used for investigating of gene transfer from clinical carbapenem-resistant K. pneumoniae isolates to a recipient E. coli J53 in vitro. Multiplex PCR & Real-time PCR was used for detection of transferab...
Examining the effects of curcumin on Drosophila ubiquitin carboxy-terminal hydrolase (duch) knockdown model
Study on anti-cancer activity of Callissia fragrans extract by YorkieS168A over-expressed fly model