Toufic Elbeaino

Toufic Elbeaino
Mediterranean Agronomic Institute of Bari | IAM-B · IPM

Ph.D in Plant Pathology, curriculum Virology

About

169
Publications
50,355
Reads
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2,383
Citations
Additional affiliations
January 2015 - present
Mediterranean Agronomic Institute of Bari
Position
  • Researcher
Description
  • Biotechnology, Plant pathogens characterization, Evolution, Genomics, Taxonomy, Host-parasite interaction, Epidemiology and Disease management
January 2010 - present
Università degli Studi di Bari Aldo Moro
January 2005 - December 2012
Mediterranean Agronomic Institute of Bari

Publications

Publications (169)
Article
Full-text available
The recent finding of Xylella fastidiosa (Xf) in olive trees in southern Italy, the scanty molecular information on this bacterium and its association with the olive quick decline syndrome (OQDS) prompted the necessity to isolate and acquire more genetic data on the type of strain present in that region. For the first time, the bacterium was isolat...
Article
Full-text available
The RNA2 of seven grapevine fanleaf virus (GFLV) isolates from vines with yellow mosaic (YM) symptoms from different origin were sequenced. These sequences showed a high variability in the homing protein (2A(HP)) and, in five of them, a putative recombination with arabis mosaic virus (ArMV) was detected. To investigate recombination frequency, the...
Article
Full-text available
California privet (Ligustrum ovalifolium Hassk.) plants exhibiting leaf yellowing, witches' broom, dieback and decline symptoms were observed for two years (2010-2011) in three gardens at Adana region (Turkey). DNA isolated from symptomatic and healthy plants was used to amplify 16S rDNA fragments by direct and nested-PCR. Phytoplasmas were detecte...
Article
Full-text available
Grapevine crown gall, caused by Allorhizobium vitis (A. vitis) and fire blight, caused by Erwinia amylovora (E. amylovora) of rosaceous plants, have been recognized as serious bacterial diseases and responsible for major crop losses in grapevine (Vitis vinifera L.), pear (Pyrus communis L.) and apple (Malus domestica Borkh.) worldwide. To develop a...
Article
Grapevine crown gall, caused by Allorhizobium vitis (A. vitis) and fire blight, caused by Erwinia amylovora (E. amylovora) of rosaceous plants, have been recognized as serious bacterial diseases and responsible for major crop losses in grapevine (Vitis vinifera L.), pear (Pyrus communis L.) and apple (Malus domestica Borkh.) worldwide. To develop a...
Article
Full-text available
The use of microbial products as natural biocontrol agents to increase a plant’s systemic resistance to viral infections is a promising way to make agriculture more sustainable and less harmful to the environment. The rhizobacterium Paenibacillus polymyxa has been shown to have strong biocontrol action against plant diseases, but its antiviral acti...
Article
Full-text available
Selmi, I., Elbeaino, T., Arezki, L., El Air, M., Digiaro, M., and Mahfoudhi, N. 2021. Prevalence and genetic diversity of grapevine virus D in Tunisia. Tunisian Journal of Plant Protection 16 (2): 19-27.
Article
Full-text available
In March 2021, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by four families (Aliusviridae, Crepuscuviridae, Myriaviridae, and Natareviridae), three subfamilies (Alpharhabdovirinae, Betarhabdovirinae, a...
Article
High-resolution melting (HRM) has shown to be reliable for the detection, discrimination, and diagnosis of several diseases of plants, animals, and humans. The aim of this research was to explore the ability to predict HRM outputs when coupled to reverse transcription quantitative polymerase chain reaction (RT-qPCR). This research used the species...
Article
A search for phytoplasma‐associated diseases was conducted for the first time in the main grapevine‐growing localities of the Dukagjini plain in Kosovo. A total of 144 samples were collected from grapevine cultivars displaying leaf yellowing, reddening, discolouration and irregular wood ripening, and analysed using nested and quantitative PCR assay...
Technical Report
Full-text available
Here we propose new names for all species of family Fimoviridae following the ICTV request to change all established species names to a now standardized binomial format.
Article
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In the 2019–2020 growing season, two corn fields located in İmamoğlu town (Adana Province, Turkey) were surveyed following the appearance of phytoplasma-like symptoms on maize plants. A total of 40 samples were collected and tested in first-round and nested PCR using universal primer pairs P1/P7 and R16F2n/R16R2, respectively. All maize-diseased pl...
Article
Forty samples representing 14 native Albanian and two foreign olive varieties were collected from an olive varietal collection plot in the Valias region (Tirana, Albania). The samples were assayed by RT-PCR for presence of olive-infecting viruses, including arabis mosaic virus (ArMV), cherry leaf roll virus (CLRV), cucumber mosaic virus (CMV), oliv...
Article
Singleplex and multiplex real-time (TaqMan®) RT-PCR assays were developed to detect seven fig-infecting viruses, i.e., fig leaf mottle-associated virus 1 (FLMaV-1), fig leaf mottle-associated virus 2 (FLMaV-2), fig mild mottle-associated virus (FMMaV), fig mosaic virus (FMV), fig latent virus 1 (FLV-1), fig cryptic virus 1 (FCV-1) and fig fleck-ass...
Article
Full-text available
p>In March 2020, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. At the genus rank, 20 new genera were added, two were deleted, one was moved, and three were renamed. At the species rank, 160 species were added, four were deleted...
Article
Full-text available
The presence of three vitiviruses associated with grapevine rugosewood disease, i.e. grapevine virus B (GVB), grapevine virus E (GVE) and grapevine virus F (GVF), was studied in 650 grapevine samples collected from eight viticulture growing provinces of Iran. RTPCR assays conducted on these samples showed that 69.5% of the grapevine material is inf...
Article
Full-text available
Fig mosaic is a viral disease (FMD) that spreads in Palestinian common fig (Ficus carica L.) orchards. Recognizing the economic value of fig plants and the harmful nature of FMD, the disease poses a significant threat to the economy of the fig production in Palestine. We applied the reverse transcription and amplification (RT-PCR) and PCR technique...
Article
We developed two real-time detection assays, TaqMan real-time PCR and LAMP, using primers and probe designed based on a sequence annotated to code for a Haemagglutinin-related protein (Hg) of Xylella fastidiosa (Xf), a gene uniquely present in the Italian olive (De Donno of olive) and American mulberry strains, for specific detection of the target...
Article
Full-text available
: Grapevine line pattern virus (GLPV) was first described 30 years ago in Hungary. The lack of its genomic sequences and of an available antiserum made its detection impossible in other parts of the world. Three different high-throughput sequencing (HTS) protocols applied on a GLPV-infected vine allowed the construction of the full genome sequence...
Article
A survey for the presence of viral infections in olive trees was conducted in spring 2018 in an olive grove of a germplasm collection located in Chania (Crete island). Forty samples were collected from the 10 most important Greek cultivars. The samples were tested for the presence of Arabis mosaic virus (ArMV), Cherry leaf roll virus (CLRV), Strawb...
Article
Full-text available
Although Greece is the world’s third largest olive production country, information about the presence of olive viruses is limited. A survey for the presence of virus infections in the ten most important Greek cultivars was conducted in a germplasm collection olive grove located in Chania, Crete. Samples were RT-PCR assayed for the presence of Arabi...
Chapter
Fimoviridae is a newly established family of plant viruses (2018) with segmented, linear, single-stranded, negative-sense (ss)RNA genomes. All species of this family share common morphological, structural, molecular and genetic features. Virus particles are approximately spherical (80–200 nm in diameter) with enveloped structures known as double-me...
Article
The mosaic disease caused by fig mosaic virus (FMV) is considered the plague of fig worldwide. A naïve phage display library, raised against the recombinant nucleocapsid protein of FMV (FMV-Np) was screened to obtain specific monoclonal recombinant antibodies in the form of single chain variable fragments (scFvs). After three rounds of biopanning,...
Article
In July 2017, a survey was conducted in a fig collection plot at Locorotondo (south of Italy) to investigate the possible presence of phytoplasmas in plants showing yellowing, deformed leaves, short internodes, mottling and mosaic. Samples were collected from symptomatic plants and tested by nested PCR assays using universal and specific primers to...
Article
Four sets of primers were designed based on the alignment of the complete coat protein (CP) gene sequences of several isolates of four different vitiviruses, i.e. grapevine virus B (GVB), GVD, GVE and GVF, and tested for their efficiency in RT-PCR assays to detect vitiviruses infections in grapevine. The resultant RT-PCR amplicons were sequenced an...
Article
Full-text available
In February 2019, following the annual taxon ratification vote, the order Bunyavirales was amended by creation of two new families, four new subfamilies, 11 new genera and 77 new species, merging of two species, and deletion of one species. This article presents the updated taxonomy of the order Bunyavirales now accepted by the International Commit...
Article
Full-text available
In October 2018, the order Bunyavirales was amended by inclusion of the family Arenaviridae, abolishment of three families, creation of three new families, 19 new genera, and 14 new species, and renaming of three genera and 22 species. This article presents the updated taxonomy of the order Bunyavirales as now accepted by the International Committe...
Article
Mosaic disease (MD), caused by Fig mosaic emaravirus (FMV), is the most important and devastating virus disease of fig trees worldwide. The detection of FMV in infected plants is possible only through the use of molecular techniques, i.e. RT-PCR and LAMP, which both offer high sensitivity of detection, but are also considered laborious when dealing...
Article
Nowadays, antibody engineering is an important approach to design and generate of therapeutic and diagnostic antibodies. The study of the interactions between antibodies and antigens is a critical step in the design of antibodies with desired properties, In silico docking analyzes is a useful tool for structural characterization of bimolecular inte...
Article
Full-text available
In a search for viral infections, double-stranded RNA (dsRNA) were recovered from a diseased cyclamen (Cyclamen persicum Mill.) accession (Cic) and analyzed by high-throughput sequencing (HTS) technology. Analysis of the HTS data showed the presence of Fig mosaic emaravirus (FMV) in this accession. The complete sequences of six FMV-Cic RNA genomic...
Article
Full-text available
Three Mediterranean Ficus carica genotypes, i.e., cultivars ‘Palazzo’, ‘Bifera nera’ and ‘Catalanisca’, initially infected by Fig leaf mottle-associated virus 1 (FLMaV-1), Fig leaf mottle-associated virus 2 (FLMaV-2), Fig mild mottling-associated virus (FMMaV), Fig mosaic virus (FMV), Fig latent virus 1 (FLV-1), Fig badnavirus 1 (FBV-1) and Fig fle...
Article
Members of the family Fimoviridae, order Bunyavirales are plant viruses with segmented, linear, single-stranded, negative-sense RNA genomes. They are distantly related to orthotospoviruses and orthobunyaviruses of the families Tospoviridae and Peribunyaviridae, respectively. The family Fimoviridae includes the genus Emaravirus, which comprises seve...
Article
Full-text available
In 2018, the family Arenaviridae was expanded by inclusion of 1 new genus and 5 novel species. At the same time, the recently established order Bunyavirales was expanded by 3 species. This article presents the updated taxonomy of the family Arenaviridae and the order Bunyavirales as now accepted by the International Committee on Taxonomy of Viruses...
Article
Full-text available
Abstract Fig mosaic virus (FMV) is considered as main causal agent of Fig Mosaic Disease complex (FMD). In order to determine the distribution and genetic diversity of nucleocapsid protein gene of FMV and better understanding the phylogenetic relationships between isolates, a number of 54 symptomatic fig leaves with chlorosis and mosaic symptoms we...
Article
Full-text available
One of the most important viral diseases of grapevine is rugose wood (RW), which is a complex of graft-transmitted viruses that affect the bark and decrease vigor and the life span of infected plants (Credi 1997, Plant Dis 81:1288-1292). One foveavirus (Rupestris stem pitting-associated virus, RSPaV) and five vitiviruses (grapevine virus A [GVA], G...
Article
Full-text available
Periwinkle (Catharanthus roseus L.) is an ornamental plant widely grown in the Mecca region in the Kingdom of Saudi Arabia (KSA). Different symptoms similar to those induced by viruses (mosaic, vein clearing, mottling, yellowing, flower variegation) prompted surveys in eight different regions of KSA to assess the presence of periwinkle-infecting vi...
Article
Full-text available
More than 100 alkaloids have been found in periwinkle, of which vincristine and vinblastine are the most notable for the treatment of diseases such as leukaemia. In this study, characterization of naturally occurring Cucumber mosaic virus (CMV) infection showed mosaic, leaf deformation and stunting of plants. Viral identification was confirmed by d...
Article
Full-text available
Seven Mediterranean Ficus carica genotypes, i.e., ‘Palazzo’, ‘Severoni precoce’, ‘Bianca’, ‘Pilusedda’, ‘Dottato bianco’, ‘Bifara’ and ‘Zidi’, were screened for the presence of seven fig-infecting viruses associated with fig mosaic disease (FMD) in order to explore their distribution in different plant organs (leaf, bud and syconium) that will be u...
Chapter
Eleven of the 15 Vitis-infecting nepoviruses are thought to have an Old World origin, either Eurasian, i.e., Grapevine fanleaf virus (GFLV); European, i.e., Arabis mosaic virus (ArMV), tomato black ring virus (TBRV), Grapevine chrome mosaic virus (GCMV), Grapevine Bulgarian latent virus (GBLV), raspberry ringspot virus (RpRSV), artichoke Italian la...
Article
Full-text available
Next-generation sequencing (NGS) allowed the assembly of the complete RNA-1 and RNA-2 sequences of a grapevine isolate of artichoke Italian latent virus (AILV). RNA-1 and RNA-2 are 7,338 and 4,630 nucleotides in length excluding the 3’ terminal poly(A) tail, and encode two putative polyproteins of 255.8 kDa (p1) and 149.6 kDa (p2), respectively. Al...
Article
Full-text available
Grapevine, considered one of the oldest horticultural crops and a highly valuable agricultural commodity in Lebanon, is cultivated over an area of ca. 11,000 ha. In autumn 2015, a survey was conducted in Ferzoul and Zahlé areas of central Bekaa (Lebanon) in three vineyards of red fruited cultivars (Red Globe, Black Pearl, and Crimson) to investigat...
Article
Full-text available
During spring 2016, a survey was carried out in Bosnian-Herzegovinian (BiH) and Montenegrin (MNE) fig orchards, germplasm collection plots and outdoor gardens, to investigate the presence of unreported fig viruses possibly present in both countries, i.e. Fig leaf mottle-associated virus 2 (FLMaV-2), Fig latent virus 1 (FLV-1), Fig cryptic virus 1 (...
Article
Mulberry badnavirus-1 (MBV-1) has been characterized as the aetiological agent of a disease observed on a mulberry tree in Lebanon (accession L34). A smallRNA NGS library was prepared and analyzed from L34 extract, and these data together with genome walking experiments have been used to obtain the full-length virus sequence. Uniquely among badnavi...
Poster
Full-text available
The rapid development of new molecular techniques has accelerated the discovery of novel to science plant-viruses including the genus Emaravirus. Currently, the genus has six confirmed species and two unclassified species, attributed to variety of symptoms. Rapid detection and an effective screening of existing and new emaraviruses is needed for de...
Article
Full-text available
Next-generation sequencing (NGS) was applied to dsRNAs extracted from an Italian pittosporum plant infected with pittosporum cryptic virus 1 (PiCV1). NGS allowed assembly of the full genome sequence of PiCV1, comprising dsRNA1 (1.9 kbp) and dsRNA2 (1.5 kbp), which encode the RNA-dependent RNA polymerase and capsid protein genes, respectively. Phylo...
Article
Full-text available
A small-scale survey was conducted on 64 beehives located in four governorates of Syria in order to assess for the first time the presence of honeybee-infecting viruses and of Varroa destructor mites in the country. RT-PCR assays conducted on 192 honeybees (Apis mellifera L.) using virus-specific primers showed that Deformed wing virus (DWV) was pr...
Article
A brief account is given of the genetic variability of Fig mosaic virus (FMV) in Tunisia. The analysis of 10 isolates from different fig cultivars and areas of the country has shown nucleotide sequence identities ranging between 90-99% in RNA-1 (302 bp analyzed), 93-99% in RNA-2 (234 bp) and 96-100% in RNA-3 (304 bp). These identity levels decrease...
Article
During May 2015, a small scale survey was conducted on 27 fig trees situated in three locations (Mostar, Trebinje and Ljubuški) in Bosnia and Herzegovina, to investigate the presence of Fig mosaic virus (FMV), Fig leaf mottle-associated virus 1 (FLMaV-1), Fig leaf mottle-associated virus 2 (FLMaV-2) and Fig mild mottle-associated virus (FMMaV). Sam...
Article
Full-text available
A recent severe outbreak of Xylella fastidiosa associated with 'olive quick decline syndrome' (OQDS) was reported in Apulia (Southern Italy). In this study an on-site real-time loop-mediated isothermal amplification (real-time LAMP) was developed for detecting X. fastidiosa in host plants and insects. A marked simplification of the DNA extraction p...
Article
Full-text available
Two Lebanese fig accessions of local varieties (Biadi and Aswad), infected by Fig leaf mottle-associated virus 1 (FLMaV-1), Fig leaf mottle-associated virus 2 (FLMaV-2) and Fig mosaic virus (FMV), were subjected to tissue culture and thermotherapy for producing virus-free plant material. The virus status of all progeny explants was assayed by RT-PC...
Article
Full-text available
Many diseases are compromising fig production in Saudi Arabia and in particular those caused by viruses. RT-PCR assays were conducted on 80 samples collected from four fig-growing provinces in the West Mecca region of Saudi Arabia, including the Fatima, Khulais, Rabigh and Alshifa valleys. Samples consisted of leaf tissues taken from caprifig and c...
Article
Full-text available
Fig leaf mottle-associated virus-1 (FLMaV-1) is a closterovirus newly identified in fig trees, in the Mecca region, suffering from mosaic disease symptoms and apparently is compromising the fig plantation in the country. In the present study, we demonstrated the efficiency of two in vivo experiments including pre and post treatments using Thuja lea...