Toto Hadiarto

• PhD
• National Research and Innovation Agency (BRIN)

One way to increase the genetic diversity of Ciherang as the superior variety is to cross Ciherang with the donor B11143D line as a New Plant Type (NPT) rice. This study aimed to obtain Ciherang X B11143D lines with the best vegetative characteristics in the field. The research was conducted in rice fields in Talagasari Village, Telagasari District...

DNA isolation is a routine procedure in molecular analysis. The method of plant genome DNA extraction has been widely available throughout global laboratories. Several labs made some modifications to obtain optimal results. This research aimed to analyse two types of DNA extraction methods for ironwood plant (Eusideroxylon zwageri), one tropical ra...

Pembungaan memegang peranan penting bagi tumbuhan karena memfasilitasi rekombinasi genetik, sehingga mendukung perkembangan keragaman genetik yang penting. Keluarga protein phosphatidylethanolamine binding proteins (PEBP) memainkan peran penting dalam mengatur waktu pembungaan dan dormansi benih di beragam spesies tanaman. Penelitian ini bertujuan...

The phosphatidylethanolamine-binding protein (PEBP) gene family plays a vital role in various aspects of plant biology, including development, differentiation, metabolism, and signaling pathways. This study examined the genetic relations between PEBP genes in palms (oil palm, coconut, and date) using various techniques of sequence retrieval, alignm...

Alternative splicing is a complex process that contributes to the generation of diverse mRNA and protein isoforms, including in oil palm (Elaeis guineensis). Despite their importance, many functions of alternative splicing genes remain poorly characterized. This study aims to investigate splicing variants of gene encoding Heading date 3a in E. guin...

As a staple food in many regions in the world, potatoes are the world’s fourth most consumed food. In Indonesia, potatoes contribute significantly to horticultural production. One major constraint known not only in Indonesia but also globally is the late blight disease caused by Phytophthora infestans. Several attempts have been developed in order...

Lodging is a major constraint in cereal crops which lead to reduction of yield and grain quality. Therefore, lodging tolerant varieties have led to remarkable harvest index increases in cereal crops. Gibberellin (GA) is important endogenous hormones responsible to plant height trait and its deficiency in plants may cause dwarfism. In this study, a...

Nitrogen use efficiency (NUE) in rice can be improved by developing a variety that absorbs and use nitrogen (N) more efficiently. We have successfully isolated the Lycopersicon esculentum alanine aminotransferase (LeAlaAT) gene from tomato, which is responsible for improved efficiency of N use in plants, and Oryza sativa alkenal reductase (OsAER1)...

Development of potato resistant to late blight caused by Phytophthora infestans was carried out by inserting RB genes from wild species potato, Solanum bulbocastanum. The genetically engineered (GE) potato Katahdin SP951 and the hybrid of Atlantic or Granola with Katahdin SP951 have been assessed by the Biosafety Technical Team (BTT) for environmen...

p>Pepper yellow leaf curl virus (PepYLCV) infection transmitted by silverleaf whitefly (Bemisia tabaci [Gennadius]) can decrease chili pepper yield up to 100%. At this moment, there is no chili pepper variety resistant to PepYLCV available. Genome editing approach through CRISPR/Cas9 is an effort to develop variety resistance to the viral infection...

Oil palm (Elaeis guineensis Jacq.) is a vegetable oil-producing crop used for consumption and biodiesel production. Fastrack breeding is one of breeding techniques to reduce plant vegetative phase significantly by a combination of conventional and transgene technologies. However, the lack information of flowering genes in oil palm become a gap for...

Recombinant plasmid construction that produces hairpin molecules for gene knockdown is not an easy method. Gateway cloning system is a technology to transfer DNA fragments utilizing clonase and specific sites. This study aimed to construct RNAi expression vector containing soluble acid invertase (SAI) gene using Gateway system to produce high-sugar...

ABSTRAK Pemanfaatan tanaman produk rekayasa genetik (PRG) telah berkembang cepat dan mendunia walaupun sampai saat ini masih terjadi kontroversi. Luas penanamannya telah mencapai 191,7 juta ha dan ditanam oleh 26 negara di lima benua. Keamanan hayati PRG secara global maupun domestik telah dijamin oleh peraturan pada tingkat undang-undang, peratura...

Abiotic stresses are non-living factors with negative morphological and physiological effects on living organisms. Substantial evidence exists that gene expression changes during plant cell growth are regulated by chromatin reconfiguration and histone modification. Several types of histone modifications are dramatically transformed in stress-respon...

The genetically engineered potato cultivar Katahdin SP951 is resistant to late blight disease, which is caused by Phytophthora infestans. The biosafety and food safety of this cultivar should be assessed prior to its commercialization. Toxicity is one of the parameters tested in the food safety evaluation of transgenics. Toxicity testing includes t...

Significance Trees’ branches grow against the pull of gravity and toward light. Although gravity and light perception have been studied in model species, much is unknown about how trees detect and respond to these signals. Here, we report the identification of a gene ( WEEP ) that controls lateral branch orientations and is directly or indirectly r...

p>Pemanfaatan tanaman kentang produk rekayasa genetik (PRG) dalam pemuliaan tanaman melalui persilangan dengan Atlantic dan Granola telah menghasilkan enam galur PRG hasil silangan yang terseleksi. Sebelum komersialisasi, kentang PRG harus dikaji keamanan pangan dan lingkungannya. Penulisan bertujuan memberikan informasi mengenai tanaman kentang PR...

p>New rice variety can be generated by means of transgenic approach. Transgenic rice researches have been conducted in many institutions worldwide using Japonica, Indica, and Javanica varieties. The most cultivated rice in Indonesia is Indica. Indica type is known to have low responsive tissues in culture and transformation media when compared to J...

p>Azospirillum sp. which has the ability for nitrogen fixation and phosphate solubilization may support modern farming in Indonesia that is mostly dependent on the usage of chemical fertilizer N, P, and K. Genetic quality of Azospirillum was improved in this research to obtain superior characters toward phosphate solubilization so that it can becom...

p>To overcome the negative impact of the fungicide application, RB gene, originated from Solanum bulbocastanum, a resistance gene to the disease has been introduced to the genome of potato plant Katahdin, which was later named as Katahdin SP951. The aims of the research were to confirm the RB gene in the genetically modified plant (GMP) Katahdin SP...

p>Protein interactions occur within cellular level of stimulated plant cells to relay signals from receptors to production of response. AtMEK1-EXGT interaction had been detected in nontreated Arabidopsis. In this research, interaction between AtMEK1, a mitogen-activated protein kinase kinase of Arabidopsis thaliana, and EXGT, endoxyloglucan transfe...

Development of potato cultivars with high levels of broad spectrum resistance is a key long-term management strategy against late blight disease caused by Phytophthora infestans. Six progeny lines of hybridization between transgenic potato Katahdin SP951 with non-transgenic Granola and Atlantic were selected based on agronomical characteristics and...

Development of potato cultivars with high levels of broad spectrum resistance is a key long-term management strategy against late blight disease caused by Phytophthora infestans. Six progeny lines of hybridization between transgenic potato Katahdin SP951 with non-transgenic Granola and Atlantic were selected based on agronomical characteristics and...

Little is known about the genetic factors controlling tree size and shape. Here, we studied the genetic basis for a recessive brachytic dwarfism trait ( dw ) in peach ( Prunus persica ) that has little or no effect on fruit development. A sequencing‐based mapping strategy positioned dw on the distal end of chromosome 6. Further sequence analysis an...

Azospirillum sp. have long been known as biofertilizer for plant growth because of its capability to produce phytohormones and fix nitrogen from the atmosphere. Multifunctional Azospirillum strain Aj Bandung 6.4.1.2 isolated in 2009 from cauliflower (Brassica oleracea) rhizosphere in Lembang, Bandung, West Java, was capable of fixing nitrogen, solu...

Azospirillum sp. have long been known as biofertilizer for plant growth because of its capability to produce phytohormones and fix nitrogen from the atmosphere. Multifunctional Azospirillum strain Aj Bandung 6.4.1.2 isolated in 2009 from cauliflower (Brassica oleracea) rhizosphere in Lembang, Bandung, West Java, was capable of fixing nitrogen, solu...

Rice (Oryza sativa L.), one of the most agronomically important crops, supplies staple food for more than half of the world's population, especially those living in developing countries. The intensively increasing world population has put a great burden on rice production. Drought as one of the major limiting factors for rice productivity has chall...

We have constructed a series of deletion mutants of Arabidopsis MAPK kinase kinase (AtMEKK1) and obtained a constitutively active mutant, AtMEKK1Delta166, which lacks in self-inhibitory sequence of N-terminal 166 amino acids but still has substrate specificity. AtMEKK1Delta166 predominantly phosphorylates AtMEK1, an Arabidopsis MAPKK, but not its d...