Toshihiro Ona

Toshihiro Ona
O'Atari Inc.

Doctor of Philosophy

About

88
Publications
8,181
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1,163
Citations
Additional affiliations
January 2002 - present
Kyushu University
Position
  • Professor (Associate)

Publications

Publications (88)
Article
Full-text available
Eucalyptus camaldulensis Dehnh. and E. globulus Labill. are economically important species for wood and pulpwood materials. Representative heights for assessing whole-tree values of cell-type proportions (vessels, fibers, ray and axial parenchyma percentages) using increment cores were examined by analysis of within-tree variations. Pattern differe...
Article
Full-text available
Conventional methods of skin stimulus evaluation for cosmetics are performed by animal tests. Alternatives to animal tests are proposed to achieve animal welfare and for example, reconstructed human epidermis (RHE) models are approved officially. However, they are based on endpoint assay using a dye causing large test errors. Furthermore, no single...
Article
Full-text available
In anti-cancer drug (candidate) screening, there is the need for evaluation at physiological concentrations similar to in vivo. This is often performed by three-dimensionally (3D) cultured cells; however, it requires a long culture period of 2 – 4 weeks with tedious experimental procedures. Here, we report on a high precision surface plasmon resona...
Article
Full-text available
Chemithermomechanical pulps (CTMP) were prepared from Eucalyptus globulus and photoirradiated, and the responses have been examined by means of ultraviolet (UV) microspectrophotometer. The absorbance in the UV spectra of fiber walls increased in the range of 300 of 350 nm as a result of photoirradiation, indicating the formation of conjugated doubl...
Article
Full-text available
The effect of alkali extraction on the lignin monomeric composition was examined inEucalyptus camaldulensis andE. globulus by thioacidolysis using extractive-free samples as a control. Results showed that the effect onEucalyptus is different among species and among sample positions in the trunk, although a small amount of lignin is solubilized duri...
Article
Full-text available
The relationship between fibre length and vessel element length was examined by their within-tree variations for breeding quality plantation pulpwood from the view points of pulp properties, that is, shorter vessel element and longer fibre are preferable. Because the genetic difference between trees may express the different ratio of fibre length t...
Article
Full-text available
Quantitative evaluation of anticancer drug efficacy using in vitro cell-based assays is useful for cancer patients, particularly those who show unconventional cancer development. Nevertheless, conventional chemosensitivity testing often requires widely used labeling agents and time-consuming laboratory procedures that provide low reliability. Label...
Article
Full-text available
We propose a rapid and label-free quantitative evaluation of anti-cancer drug efficacy in apoptosis using live cancer cells cultured on a sensor chip. The resultant custom-made, high-precision surface plasmon resonance (SPR) sensor monitors the inner mitochondrial membrane's potential change (DeltaPsi(m)). For trans-membrane anti-cancer drugs to be...
Article
Full-text available
Conventional methods used to analyze total fatty acids in wood generally require tedious and time-consuming pretreatments such as solvent extraction and hydrolysis of esterified fatty acids of fats. In this study, both total fatty acid composition and the yield from free and esterified fatty acids of fats in Acacia species were investigated using a...
Article
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We investigated the feasibility of using several fast-growing tropical or subtropical hardwood species for timber production by measuring key wood qualities in relationship to the high rates of lateral growth. The trees tested were sampled from even-aged plantations of Acacia mangium, A. auriculiformis, hybrid Acacia (A. mangium × A. auriculiformis...
Article
Cellobiose, a structural subunit of cellulose, was utilized as a retention promoter of polymer additives to a cellulose matrix in an aqueous papermaking system. beta-D-Cellobiosylamine was conjugated with anionic polyacrylamide (A-PAM) through a condensation reaction using water-soluble carbodiimide. Glyco-modified A-PAM containing cellobiose resid...
Article
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Direct dye with a high affinity for cellulose substrate was utilized as a cellulose anchor to promote retention of paper strengthening additives under various conditions associated with the wet end of a paper machine. Direct Red 28 (DR) was covalently linked to anionic polyacrylamide (A-PAM) via a condensation reaction using water-soluble carbodiim...
Article
Full-text available
An in vitro, rapid, and quantitative cell-based assay is needed to predict the efficacy of cancer drugs in individual patients, because a cancer patient may have unconventional aspects of tumor development. Here we report a rapid and label-free quantitative method for verifying apoptosis in living cancer cells cultured on a sensor chip with a newly...
Article
Full-text available
In vitro rapid and quantitative cell-based assay is demanded to verify the efficacy prediction of cancer drugs since a cancer patient may have unconventional aspects of tumor development. Here, we show the rapid and non-label quantitative verifying method and instrumentation of apoptosis for cell cycle-arrest type cancer drugs (Roscovitine and D-al...
Article
Full-text available
Utilization of rapidly growing trees, such as eucalypts, for high-yield mechanical pulps is limited by low brightness owing to high contents of alkali and neutral extractives. Wood supply problems have developed in many areas of the world and new sources of high-yield pulp are needed. Ten Eucalyptus globulus trees were selected from two plantation...
Article
Full-text available
Thermochemolysis-gas chromatography in the presence of tetrabutylammonium hydroxide was applied to the direct determination of phenolic compounds present as “extractives” in a wood sample (Eucalyptus camaldulensis). The phenolic extractives converted into their perbutyl derivatives were separated from the other phenolic products derived from the li...
Article
Full-text available
Within-tree variations of derived wood properties of Runkel ratio, Luces shape factor, slenderness ratio, and solids factor were examined for Eucalyptus camaldulensis and Eucalyptus globulus trees and the tendency difference in the within-tree variations between individuals and between species, in both radial and axial directions by statistical dat...
Article
Wet-end application of direct dye and cello-oligo-saccharide with a high affinity for cellulosic substrate was investigated with regard to retention behavior of polymer additives under various wet-end conditions. Either Direct Red 28 (DR28) or cellobiosylamine (CB) was covalently introduced into anionic polyacrylamide (A-PAM); called DR-A-PAM or CB...
Article
The industrial recycling rate is limited to about three times; however, tree breeding program has never aimed for paper recycling. The first purpose of our study here was to examine the feasibility of selection of trees with high performance for kraft paper recycling from plantation trees even showing similar growth and pulp properties after digest...
Article
Full-text available
The representative heights in the trunk to indicate whole-tree values and the within-tree variations of derived wood properties, namely flexibility coefficient, wall coverage ratio, vessel diameter radial/tangential (R/T) ratio, fiber diameter R/T ratio, and fiber coarseness, were examined in Eucalyptus camaldulensis and E. globulus trees. In both...
Article
Full-text available
Length analysis of vessel elements in tree trunks used for water and nutrient transport is a lengthy, multistep procedure although it reflects environmental stresses on a tree. The feasibility of using FT-Raman spectroscopy for rapid determination of vessel element length in a tree was examined using wood powders of two Eucalyptus species, includin...
Article
The feasibility of physical property assessment of waterborne automotive paint emulsion system using Fourier transform Raman spectroscopy (FT-Raman spectroscopy) was examined for viscosity as an example trait. The experiment was performed under the same temperature both in conventional and Raman spectroscopic methods aiming the online control. Rama...
Article
Full-text available
The occurrence of heart splitting during the crosscutting of logs was discussed in relation to the released strain on Eucalyptus spp. logs. The strains released in the longitudinal and tangential directions were measured by the strain-gauge method and were correlated with the length of the heart split measured on the same logs. There were differenc...
Article
We examined the effects of polyphenols on the analysis of lignin by histochemical methods, namely, the Mäule color reaction coupled with microspectrophotometry and ultraviolet microspectrophotometry, in wood of Eucalyptus camaldulensis and E. globulus. Thin sections and wood meals were extracted with solutions of alkali at different concentra-tions...
Chapter
Full-text available
Within-tree variation and the representative height in the trunk to indicate whole-tree values of fibre morphology, including radial, tangential and average diameters, wall thickness, and lumen diameter, were examined using Eucalyptus camaldulensis and E. globulus trees. In E. camaldulensis, high within-tree variation was generally observed in the...
Article
Full-text available
Representative heights in the trunk to determine whole-tree values of vessel morphology (radial, tangential and average diameters, and wall thickness), and vessel frequency, were evaluated by examining the within-tree variation in Eucalyptus camaldulensis and E. globulus trees. In both species, vessel morphology value was observed to Increase in th...
Chapter
The principle of paper recycling is based on that the inter-fiber bonding can be broken by hydration to isolate individual pulp fiber; that is, the sheet forming using water is a reversible process. It is interrogative whether every aspect in recycling is reversible, preserving the level of paper-making properties. It is obvious that most of paper...
Chapter
Acacia species are widely used for raw materials of papers [1]. However, fatty acid components remaining in paper often cause the paper slide on a printer drum. For this reason, rapid characterization of total fatty acid components in wood is required for the selective tree breeding of Acacia species with less fatty acid content.
Chapter
Woody plants develop special tissues, which are called reaction wood, on the inclined stems or branches. Tension wood, reaction wood for hardwoods, can be characterized by the presence of gelatinous (G) -fibers which contain a thickened cellulosic inner layer (G-layer) [1–3]. However, some species of primitive hardwoods have been reported to freque...
Chapter
The concentration of atmospheric carbon dioxide is increasing and becoming a major problem. After the Kyoto protocol, afforestation is gaining support as one of the best solutions to solve this problem. Creating forest resources which are valuable for industry is an important factor to sustain afforestation and to maintain healthy forests. The recy...
Chapter
Eucalyptus camaldulensis plays an important role as one of the major plantation species for pulpwood especially in sub-tropical regions. Tree selection for high pulp yield and strength in the management of pulpwood forests has attracted interest in the use of plantations with short term rotations to reduce the pulp cost and atmospheric CO2 [1]. The...
Chapter
Aucalyptus is a widely utilized species in tree plantation, especially intended for pulp production [1]. E. globulus is one of the fast growing species in tempertae zone with a rotation period of 8–10 years [2]. We have reported that chemithermomechanical pulp (CTMP) paper prepared from several individuals of E. globulus showed high brightness (abo...
Chapter
Acacia species are widely planted for pulp production in south-east Asian countries. It is well known that pulp properties strongly relate to cell morphology of wood [1–3]. In the present study, radial variation of cell morphology (diameter, wall thickness and length in vessel element and wood fiber, and volume of cell types) in two Acacia species...
Chapter
Remarakable progress in microscopic techniques from the 1950’s to the 1960’s enabled plant anatomists to clearly describe the fine structure of the wood cell wall. Nowadays, it is our common knowledge that each lamella can be approximated by a “two-phase structure”, i.e. the reinforcing element of cellulose microfibril (CMF) and the matrix (MT) sub...
Chapter
Eucalyptus is one of the most widely utilized species for tree plantation, especially intended in the use for chemical pulp production [1]. E. globulus is a fast growing species in a moderate zone with an 8–10 year-rotation period [2]. The tree breeding programs are aiming to improve growth, pulp yield and strength in pulp and paper products becaus...
Chapter
Potassium ion (K+) is the most abundant cation inside prokaryotic and eukaryotic cells and the K+ transport system in biological membranes is essential for maintenance of membrane potential and diverse cellular processes in all organisms studied thus far. Bacterial K+ uptake systems have been investigated extensively. In E. coli, three kinds of K+...
Chapter
Tree breeding has become more important as fixation sources of atmospheric CO2, since the global warming has been called a serious environmental problem. Therefore, the selection of elite trees possessing significant CO2 absorption ability together with excellent pulp properties is strongly needed to accelerate both atmospheric CO2 absorption and p...
Chapter
Many Eucalyptus plantations have been developed in the tropical and sub-tropical regions. Eucalyptus globulus is one of the fast-growing species planted in temperate regions and its normal rotation period is 8–10 years. It is suitable for kraft pulping because of its high pulp yield and the tree plantation is expected to solve the pulpwood supply s...
Chapter
In lignin structural analysis, 1H-nuclear magnetic resonance (NMR) spectroscopy is frequently applied using dehydrogenative polymer (DHP) [1]. However, this technique has a disadvantage of long measurement time. On the contrast, Fourier transform (FT) Raman spectroscopy has an advantage of short measurement time and gives the information on various...
Chapter
After the Kyoto Protocol, considerable attentions have been paid on forests in the context of climate change. Forests have a key role in balancing society’s need for wood-based products and protecting our environment by increasing net carbon sequestration in terrestrial carbon sinks removing atmospheric CO2. The sustainably managed forests area is...
Chapter
The cuttings from some old Eucalyptus trees have inferior ability to root, leading to difficulties to multiply plus or elite clones from them. The main cause for this phenomenon is attributed to polyphenols contained in those trees. In the present study, polyphenols contained in E. camaldulensis cuttings or dissolved in water after scission of the...
Chapter
Recently, physiological activity of phenolic compounds received significant attention; for example; antiproliferative effect on cancer cells has been reported in quercetin [1], which is contained in a bark of Eucalyptus species and in a propolis [2, 3]. However, the mechanism of phenolic compounds against cells is very complicate. Furthermore, scre...
Book
It is an honor and pleasure for me to write the foreword of this book comprising the of Forest Resources for proceedings of the Fourth Symposium on the Improvement Recyclable Forest Products. The symposium was organized by Dr. Toshihiro Ona, Associate Professor at the Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University,...
Article
Full-text available
A novel rapid and sensitive method to characterize wood extractives in wood samples was developed on the basis of reactive thermal desorption-gas chromatography (RTD-GC) in the presence of an organic alkali, tetramethylammonium acetate ((CH3)4N+OCOCH3−, TMAAc) without using any cumbersome pretreatment such as solvent extraction. The RTD at 200 °C u...
Article
Full-text available
To assess kraft pulp properties produced from Eucalyptus trees, the feasibility of using Fourier transform - Raman spectroscopy for rapid non-destructive quantification of wood constituents, anatomy and basic density was examined. Wood constituents such as contents of holocellulose, α-cellulose, hemicellulose, lignin, extractives, lignin syringyl/g...
Article
Vibrational spectroscopy is utilized to characterize compounds, but it has never been applied to quantitative morphological analysis of polymers in a mass. By using near-infrared Fourier transform Raman spectroscopy coupled with multivariate data analysis, the average particle diameter of macroscopic size in water-based latex emulsions was successf...
Article
Detailed quantitative analysis of lignin monomer composition comprising p-coumaryl, coniferyl, and sinapyl alcohol and p-coumaraldehyde, coniferaldehyde, and sinapaldehyde in plant has not been studied from every point mainly because of artifact formation during the lignin isolation procedure, partial loss of the lignin components inherent in the c...
Article
Full-text available
A plant hyperpolarization-activating K+ channel, KAT1, is highly selective for K+ over Na+ and is little affected by external Na+, which is crucial to take up K+ effectively in a Na+-containing environment. It has been shown that a mutation at the location (Thr256) preceding the selectivity signature sequence dramatically enhanced the sensitivity o...
Article
Full-text available
Near-infrared Fourier transform Raman (FT) spectroscopy is a powerful analytical method, but, quantitative analysis is often difficult owing to changes in the environmental temperature. The feasibility of using a commercial FT-Raman spectrometer (equipped with an InGaAs detector operated at room temperature) in a thermal box to obtain ±0.1 °C with...
Article
Pyrolysis-gas chromatography (Py-GC) using a vertical microfurnace pyrolyzer combined with the principal component analysis (PCA) was applied to the discriminative analysis of Eucalyptus camaldulensis trees grown at the same field from the seeds of different origins. On the pyrograms of the Eucalyptus samples obtained at 450°C, many characteristic...
Article
Full-text available
 Relationships between cell and pulp properties were investigated by examining the within-tree property variations in Eucalyptus camaldulensis and Eucalyptus globulus. Properties investigated included proportions of ray and axial parenchyma, thickness of cell walls and cell wall percentages. The characteristics of the ray and axial parenchyma (thei...
Article
The feasibility of using FT-Raman spectroscopy for rapid non-destructive determination of wood constituents and anatomy was examined using Eucalyptus species, including samples of various ages and colors. As wood constituents, contents of holocellulose, σ-cellulose, hemicellulose, lignin, extractives, alkali extractives and total extractives, ligni...
Article
The repeated wetting and drying of a recycling process affects the strengths of papers from recycled pulps, as well as the micro-structure of the pulp fibres. Relationships between pulp fibre and handsheet properties during recycling were assessed by using thirty-three individual trees of Eucalyptus globulus. The shrinkage of internal pore structur...
Article
A series of thirty-three 9-year-old Eucalyptus globulus trees planted in southwest Australia were examined to investigate variations in pulp fibre and handsheet properties during recycling. Each pulp refined for 7,500 rev. in the PFI mill was subjected to recycling of a sequence of wetting, defibration, dewatering and drying up to five cycles. Rest...
Article
Full-text available
The feasibility of using Fourier transform Raman spectroscopy for rapid assessment of various pulp properties was examined using two Eucalyptus species, including samples of various ages and colours, which are of importance as a plantation source. Pulp properties, namely pulp yield, sheet density, burst factor, breaking length, tear factor, unbleac...
Article
Full-text available
Vibrational spectroscopy is utilized to determine compounds. However, it has never been applied to quantify architecture of multicell biological specimens. By using Fourier transform Raman spectroscopy coupled with multivariate data analysis and wood meal samples of Eucalyptus camaldulensis and E. globulus trees, cell width and cell wall thickness...
Article
Full-text available
Analysis of cell morphology in native wood is a lengthy, multistep procedure, although it has a marked effect on product quality and the use of wood. The feasibility of using FT-Raman spectroscopy for rapid determination of cell length and cell wall ratio as cell morphology in native wood was examined with the use of wood meals of two Eucalyptus sp...
Article
The first isolation of plant protoplast using the enzyme β-1,3-glucanase was successfully achieved using sterile Acacia mangium as plant source. Aniline blue reaction and antibodies highly specific to β-1,3-glucans confirmed the induction of P-1,3-glucans in the cell wall by wounding during the mincing treatment which would enhance the accessibilit...
Article
Full-text available
Pyrolysis–gas chromatography (Py-GC) using a vertical microfurnace pyrolyzer was applied to the precise determination of the ‘within-tree variation’ of the ratio of syringyl and guaiacyl units (S/G ratio) for lignin in Eucalyptus camaldulensis. On the pyrogram of the Eucalyptus obtained at 450 °C, many characteristic peaks derived from syringyl and...
Article
Analysis of the proportion of cell types in native wood is important for understanding the environmental stresses including an increasing atmospheric CO2 concentration on the structure of wood, especially for the management of plantation forests which will reduce our reliance on natural forests. The conventional method for determining the proportio...
Chapter
Analysis of proportion of cell types in native wood is important to understand the environmental stresses including an increasing atmospheric CO2 concentration on the structure of wood, especially for the management of plantation forest which will reduce our reliance on natural forests. The conventional method to determine the proportion of cell ty...
Article
Full-text available
The feasibility of using FT-Raman spectroscopy for rapid determination of wood basic density was examined for two Eucalyptus species, including samples of various ages and colors, which are of importance as a plantation source. Wood basic density is an important indicator of wood physical properties, pulp properties and pulpwood productivity. Measu...
Article
Full-text available
Relations between various extracted basic densities and wood chemical components were investigated by their within-tree variations in Eucalyptus globulus for assistance in the prediction of the properties of wood or wood-derived products. Extraneous compounds affect the relations between various basic densities and wood chemical components such as...
Article
Full-text available
The feasibility of using FT-Raman spectroscopy for rapid non-destructive determination of hemicellulosic neutral sugar composition, important factors for pulp properties, in native wood was examined using two Eucalyptus species, including samples of various ages and colors, which are important sources of plantation pulpwood. The sugars, glucose, xy...