Torben Vedel Borchert

Torben Vedel Borchert
Novozymes · Protein Engineering

33.62
 · 
Ph.D., M.S.

About

64
Publications
8,992
Reads
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3,863
Citations
Introduction
Skills and Expertise
Biotechnology
Enzymes
Protein Engineering
Enzyme Activity
Protein Purification
Enzymology
Proteins
Protein Characterization
Protein Biochemistry
Cloning
Research Experience
February 1991 - October 1993
European Molecular Biology Laboratory
  • Structural and Computational Biology Unit (Heidelberg)
  • Heidelberg, Germany
Position
  • PostDoc Position

Publications

Publications (64)
Enzymes in Industrial Biotechnology
Chapter
  • Jun 2016
Pectate lyase variants
Patent
  • Apr 2015
Three-dimensional structure of a variant `Termamyl-like' Geobacillus stearothermophilus α-amylase at 1.9 Å resolution
Article
Full-text available
  • Jan 2015
Alpha-amylase variants
Patent
  • Nov 2014
Galactanase variants
Patent
  • Feb 2013
Toward Fast Determination of Protein Stability Maps: Experimental and Theoretical Analysis of Mutants of a Nocardiopsis prasina Serine Protease
Article
  • Jun 2012
Activity of three β-1,4-galactanases on small chromogenic substrates
Article
  • May 2011
Structural and Biochemical Studies Elucidate the Mechanism of Rhamnogalacturonan Lyase from Aspergillus aculeatus
Article
  • Nov 2010
Structure of a pullulanase from Bacillus acidopullulyticus
Article
  • Aug 2009
Substrate recognition and catalysis of polysaccharide lyases
Article
Full-text available
  • Aug 2009
Investigating the binding of β-1,4-galactan to Bacillus licheniformis β-1,4-galactanase by crystallography and computational modeling
Article
  • Jun 2009
Understanding How Diverse β-Mannanases Recognize Heterogeneous Substrates
Article
  • Jun 2009
Between-Species Variation in the Kinetic Stability of TIM Proteins Linked to Solvation-Barrier Free Energies
Article
  • Nov 2008
New insight into catalytic mechanism of serine proteases from ultra-high resolution X-ray studies
Article
  • Aug 2008
Structure and mechanism of rhamnogalacturonan lyases
Article
Full-text available
  • Aug 2008
Comparison of the structures and the crystal contacts of trypanosomal triosephosphate isomerase in four different crystal forms
Article
  • May 2008
Crystallographic and kinetic studies of rhamnogalacturonan lyase from Aspergillus aculeatus
Article
  • Aug 2007
Beyond Lumry-Eyring: An unexpected pattern of operational reversibility/irreversibility in protein denaturation
Article
  • Jul 2007
Characterization and Three-dimensional Structures of Two Distinct Bacterial Xyloglucanases from Families GH5 and GH12
Article
Full-text available
  • Jul 2007
Industrial Enzymes
Article
  • Feb 2007
Role of Solvation Barriers in Protein Kinetic Stability
Article
  • Aug 2006
Directed evolution of enzyme stability
Article
  • Jul 2005
Overexpression of trypanosomal triosephosphate isomerase in Escherichia coli and characterisation of a dimer-interface mutant
Article
  • Mar 2005
Structure of a Bacillus halmapalus family 13 ά-amylase, BHA, in complex with an acarbose-derived nonasaccharide at 2.1 A resolution
Article
Full-text available
  • Mar 2005
Enzymes for Technical Applications
Chapter
  • Jan 2005
Kirk-Othmer Encyclopedia of Chemical Technology
Chapter
Full-text available
  • Aug 2004
Structure of two fungal β-1,4-galactanases: Searching for the basis for temperature and pH optimum
Article
  • Jul 2003
Purification, crystallization and preliminary X-ray crystallographic studies on acetolactate decarboxylase
Article
Full-text available
  • Jul 2003
Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently
Article
Full-text available
  • Jan 2003
Industrial enzyme applications. Curr Opin Biotechnol
Article
Full-text available
  • Sep 2002
Conformational Averaging in pK Calculations: Improvement and Limitations in Prediction of Ionization Properties of Proteins
Article
  • Aug 2001
The determinants of α-amylase pH-activity profiles
Article
  • Aug 2001
In vitro selection of enzymatically active lipase variants from phage libraries using a mechanism-based inhibitor
Article
  • Aug 2001
The determinants of -amylase pH-activity profiles
Article
Full-text available
  • Jul 2001
Protein engineering of bacterial α- amylase
Article
  • Dec 2000
Efficient gene targeted random mutagenesis in genetically stable Escherichia coli strains
Article
Full-text available
  • Dec 2000
Design and synthesis of triglyceride analogue biotinylated suicide inhibitors for directed molecular evolution of lipolytic enzymes
Article
  • Oct 2000
Structural Analysis of a Chimeric Bacterial α-Amylase. High-Resolution Analysis of Native and Ligand Complexes † , ‡
Article
  • Sep 2000
Conversion of the maltogenic ??-amylase Novamyl into a CGTase
Article
  • Aug 2000
Conversion of the maltogenic -amylase Novamyl into a CGTase
Article
Full-text available
  • Jul 2000
A novel biotinylated suicide inhibitor for directed molecular evolution of lipolytic enzymes
Article
  • Apr 2000
Display of active subtilisin 309 on phage: Analysis of parameters influencing the selection of subtilisin variants with changed substrate specificity from libraries using phosphonylating inhibitors
Article
  • Mar 2000
Electrostatics in the active site of an α-amylase
Article
  • Oct 1999
DNA shuffling of subgenomic sequences of subtilisin
Article
  • Oct 1999
X-ray Structure of Novamyl, the Five-Domain “Maltogenic” α-Amylase from Bacillus stearothermophilus : Maltose and Acarbose Complexes at 1.7 Å Resolution † , ‡
Article
  • Jul 1999
Development of Industrially Important .ALPHA.-Amylases.
Article
  • Jan 1999
Oligosaccharide specificity of a family 7 endoglucanase: Insertion of potential sugar-binding subsites
Article
  • Oct 1997
Oxidation stable amylases for detergents
Article
  • Dec 1995
An interface point-mutation variant of triosephosphate isomerase is compactly folded and monomeric at low protein concentrations
Article
  • Aug 1995
Identification of a Bacillus subtilis secretion mutant using a -galactosidase screening procedure
Article
  • Aug 1995
Three new crystal structures of point mutation variants of mono TIM: conformational flexibility of loop-1, loop-4 and loop-8
Article
  • Jul 1995
Modular mutagenesis of a TIM-barrel enzyme: the crystal structure of a chimeric E. coli TIM having the eighth beta alpha-unit replaced by the equivalent unit of chicken TIM
Article
  • Sep 1994
The crystal structure of human CskSH3: Structural diversity near the RT-Src and n-Src loop
Article
  • Apr 1994
Design, Creation, and Characterization of a Stable, Monomeric Triosephosphate Isomerase
Article
  • Mar 1994
Replacing the (beta alpha)-unit 8 of E.coli TIM with its chicken homologue leads to a stable and active hybrid enzyme
Article
  • Dec 1993
The crystal structure of an engineered monomeric triosephosphate isomerase, monoTIM: the correct modelling of an eight-residue loop
Article
  • Dec 1993
Replacing the (βα)-unit 8 of E.coli TIM with its chicken homologue leads to a stable and active hybrid enzyme
Article
  • Nov 1993
Selective Interaction of Glycosomal Enzymes from Trypanosoma brucei with Hydrophobic Cyclic Hexapeptides
Article
Full-text available
  • Oct 1993
Overexpression of trypanosomal triosephosphate isomerase in Escherichia coli and characterisation of a dimer-interface mutant
Article
Full-text available
  • Mar 1993
Crystallographic binding studies with triosephosphate isomerases: Conformational changes induced by substrate and substrate-analogues
Article
  • Aug 1992
Levansucrase: a tool to study protein secretion in Bacillus subtilis
Article
  • Dec 1991
Effect of signal sequence alterations on export of levansucrase in Bacillus subtilis
Article
Full-text available
  • Feb 1991
STRUCTURE-FUNCTION STUDIES ON THE BACILLUS AMYLOLIQUEFACIENS LEVANSUCRASE SIGNAL PEPTIDE
Chapter
  • Dec 1990
Isolation and characterization of levansucrase-encoding gene from Bacillus amyloliquefaciens
Article
  • Dec 1990