Tomasz Wielkoszyński

Tomasz Wielkoszyński
Medical University of Silesia in Katowice | SUM · Department of Chemistry

About

98
Publications
11,252
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,441
Citations
Citations since 2016
13 Research Items
711 Citations
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140

Publications

Publications (98)
Article
Full-text available
Background: The purpose of this study was to estimate the effect of whole-body cryostimulation (WBC) and subsequent kinesiotherapy on inflammatory endothelium and oxidative stress parameters in healthy subjects. Methods: The effects of ten WBC procedures lasting 3 min per day and followed by a 60-min session of kinesiotherapy on oxidative stress...
Article
Introduction: Oxidative stress has been implicated in pathogenesis of thyroid-associated orbitopathy (TAO) in patients with Graves' disease (GD). This study assessed the effect of thyroid hormone abnormalities on selected antioxidant parameters of in patients with active TAO. Material and methods: The study group consisted of 56 patients with GD...
Article
Full-text available
Objective: Oxidized cholesterol derivatives are compounds with proven atherogenic and mutagenic effects. However, little is known about the effect of oxidized plant sterol derivatives (oxyphytosterols), whose structure is similar to the one of oxycholesterols. Our previous studies indicate that they have a similar profile of action, e.g., both exa...
Article
Full-text available
Objective: Cholesterol oxidation products have an established proatherogenic and cytotoxic effect. An increased exposure to these substances may be associated with the development of atherosclerosis and cancers. Relatively little, though, is known about the effect of phytosterol oxidation products, although phytosterols are present in commonly ava...
Article
Full-text available
Objective: The aim of the study was to estimate the impact of whole-body cryotherapy (WBC) and subsequent kinesiotherapy on oxidative stress and lipid profile when performed in a closed cryochamber on healthy subjects. Material and methods: The effect of ten WBC procedures lasting 3 minutes a day followed by a 60-minute session kinesiotherapy on...
Article
Full-text available
Pregnancy-associated plasma protein A (PAPP-A) is a high-molecular zinc-binding metalloproteinase that was first detected in the serum of pregnant women. It can also be detected in men and non-pregnant women. Recently, a growing interest in determining the concentration of PAPP-A as a marker of oxidative stress and atherosclerotic processes has bee...
Article
Full-text available
Introduction: Even though uremic pruritus (UP) is very troublesome for haemodialysis (HD) patients, its underlying mechanism is not fully understood. Aim: Due to the possible role of brain-derived neurotrophic factor (BDNF) and its higher serum concentration in haemodialysis diabetic patients compared to non-diabetic ones, this study is aimed to...
Article
Full-text available
Yersiniosis is a foodborne infection caused by Yersinia enterocolitica or Yersinia pseudotuberculosis. Although yersiniosis is most often self-limiting, some patients develop chronic infections, such as reactive arthritis, glomerulonephritis, or myocarditis, which require an antibiotic treatment. Whereas early infections can be diagnosed by direct...
Article
Full-text available
Objective The aim of the study was to estimate the impact of whole body cryotherapy (WBC) on oxidative stress when performed in a closed cryochamber on patients with ankylosing spondylitis (AS). Material and methods The effect of ten WBC procedures lasting 3 minutes a day with a subsequent 60-minute session kinesiotherapy on oxidative stress in ma...
Article
Full-text available
Objective Oxidized cholesterol derivatives are thought to exert atherogenic effect thus adversely affecting vascular endothelium. The aim of the study was to assess the effect of 5α,6α-epoxycholesterol on experimentally induced hypercholesterolemia in rabbits, and the levels of homocysteine (HCY), asymmetric dimethylarginine (ADMA), paraoxonase-1 (...
Article
Full-text available
Objective The aim of the study was to estimate the impact of whole-body cryotherapy (WBC) on cardiovascular risk factors in patients with ankylosing spondylitis (AS). Material and Methods We investigated the effect of WBC with subsequent kinesiotherapy on markers of inflammation, oxidative stress, lipid profile, and atherosclerosis plaque in male...
Article
Full-text available
Objective The primary aim of the study was to assess levels of oxidative stress markers, soluble CD40 ligand (sCD40L), serum pregnancy-associated plasma protein-A (PAPP-A), and placental growth factor (PlGF) as well as carotid intima-media thickness (IMT) in patients with ankylosing spondylitis (AS) with active phase without concomitant classical c...
Article
Full-text available
Background. Whole-body cryostimulation (WBC) is the therapeutic exposure of the total human body (without underwear) to a very low temperature (below –100°C) for 120–180 s. Currently, WBC is used more frequently not only in the treatment of patients suffering from various diseases, but also by healthy people as a wellness method. Objectives. The ai...
Article
Full-text available
We hypothesized that addition of substances with antioxidant activity could decrease the concentrations of biomarkers of oxidative stress and inflammatory process, thus inhibiting nonalcoholic steatohepatitis development. We investigated the influence of α -lipoic acid (ALA) and garlic administration on the development of adverse changes in rabbit...
Article
Full-text available
W S T Ę P: Celem niniejszych badań było opracowanie metody oznaczania aktywności pepsyny w soku żołądkowym na podstawie metody opisanej w piśmiennictwie, która w badaniach laboratoryjnych okazała się nie w pełni efektyw-na. Podjęta praca jest próbą udoskonalenia metody polegającej na trawieniu hemoglobiny wołowej przez pepsynę i wprowadzenia skutec...
Article
Wstęp Celem niniejszych badań było opracowanie metody oznaczania aktywności pepsyny w soku żołądkowym na podstawie metody opisanej w piśmiennictwie, która w badaniach laboratoryjnych okazała się nie w pełni efektywna. Podjęta praca jest próbą udoskonalenia metody polegającej na trawieniu hemoglobiny wołowej przez pepsynę i wprowadzenia skutecznych...
Article
Full-text available
Ceruloplasmin (Cp) is the major antioxidant in plasma, a protein that carries about 95% of the total copper. Multiple biochemical activities of ceruloplasmin have been described, including copper transport or oxidation of Fe(II) to Fe(III) for subsequent uptake by transferrin and ferritin. Reduction of the pool of free Fe(II) ions by Cp prevents th...
Article
Full-text available
The aim of the present study was to estimate the ability of individual phenolic acids to eliminate Fe(II) ions from the solution. Moreover, the influence of phenolic acids on the ferroxidase activity of ceruloplasmin (Cp) isolated from blood plasma of healthy volunteers (CpC) and patients with atherosclerosis obliterans (CpAO) was established in vi...
Article
Full-text available
The role of non-enzymatic antioxidants, such as uric acid, albumin, bilirubin, and α-tocopherol, in lead poisoning remains unclear. Therefore, the aim of the study was to explore the association between occupational exposure to lead and non-enzymatic antioxidant concentrations in serum and plasma. The study population consisted of 278 healthy male...
Article
Full-text available
Objective: Although medicine students express positive attitudes toward providing lifestyle counseling, they require more instruction in many areas of health behavior in order to be helpful to their patients. The presented study included the students' questionnaires analysis regarding their lifestyle and exposure to tobacco smoke. The aim of this...
Article
Full-text available
The aim of the study was to investigate the influence of occupational lead (Pb) exposure on lipid peroxidation, protein carbonylation, and plasma viscosity in workers. The examined group included 283 healthy male employees of manufacturing facilities using zinc and Pb. The mean blood concentrations of Pb and zinc protoporphyrin as well as the mean...
Article
The chemical variety of tobacco smoke components induces a multidirectional, not yet fully understood effects on living organisms, including both those actively and passively exposed. The chemical compounds present in tobacco smoke, due to its heterogeneous structure and properties undergo different biotransformation processes. Some components of t...
Article
Full-text available
Oxidative stress is one of several factors which contribute to the development of colorectal carcinogenesis. The aim of the study was an assessment of the activity of antioxidant enzymes in tumour and corresponding normal distal mucosa in a group of patients with colorectal adenocarcinoma. Samples of tumour and corresponding normal mucosa were obta...
Article
Theophylline, popular diuretic, is used to treat asthma and bronchospasm. In blood it forms complexes with albumin, which is also the main transporter of fatty acids. The aim of the present study was to describe the influence of fatty acids (FA) on binding of theophylline (Th) to human serum albumin (HSA) in the high affinity binding sites. Binding...
Article
Introduction: Borrelia burgdorferi s.l. is a tick-transmitted spirochaete, which can cause acute or chronic infection, either symptomatic or occult. The result depends on spirochaete species' interaction with individual human immunity. Material and methods: The survey was conducted in the group of 1104 Silesia inhabitants (age 3-96 years; mean 44.9...
Article
Negative attitudes in health such as cigarette smoking and imbalanced diet play important role in pathogenesis of various diseases. Cigarette smoking constitutes one of the main sources of exposure to cancerogenic and procancerogenic xenobiotics among adults as well as among young people. Many studies have proven that cigarettes smokers more freque...
Article
Full-text available
The effects of administration of oxidized rapeseed oil and α-lipoic acid on activities of blood antioxidant enzymes and malondialdehyde (MDA) concentration were studied in laboratory rats fed a high-fat diet. Addition of oxidized oil resulted in increased production of oxygen radicals, evidenced by elevated plasma MDA production. Such effect was co...
Article
The aetiopathogenesis of psoriasis is very complex and has not been well known. Recently, it has been proposed that mechanisms dependent on free radicals may be involved in the development of this dermatosis. Also, psoriasis coincides with lipid disturbances, diabetes and diseases of cardiovascular system. However, the common mechanism connecting t...
Article
Introduction: Dyslipidaemia occurring concomitantly with psoriasis and proatherogenic serum lipid profile have been observed in multiple studies of middle-aged subjects. Modifications of lipid concentration indices are associated with increased risk of atherosclerosis observed in the course of psoriasis. Aim: To evaluate the impact of phototherapy,...
Article
Full-text available
Liver ischaemia and reperfusion (IR) injury is a significant clinical problem. The aim of our study was to investigate the protective effect of tumor necrosis factor-alpha (TNF-alpha) on rat liver ischaemia-reperfusion injury. A TNF-alpha dose of 3 microg/kg body weight was injected into rats that had undergone partial (70%) ischaemia and reperfusi...
Article
Full-text available
The effects of administration of oxidized rapeseed oil and α-lipoic acid on activities of hepatic antioxidant enzymes and lipid peroxidation were studied in laboratory rats. There was an increase of the activities of superoxide dismutase, glutathione peroxidase, glutathione reductase, and glucose-6-phosphate dehydrogenase in rats fed a high fat die...
Article
Cigarette smoking constitutes one of the main sources of exposure to cancerogenic and procancerogenic xenobiotics in the human population. Smoking is not only a bad habit among adults but also among young people. Despite many anti-tobacco campaigns undertaken by European Union countries still the smoking percentage among young people is high and do...
Article
Heat shock proteins (Hsp) are highly expressed in many malignant human tumors, including tumors of the genital tract. One of the Hsps strongly associated with the process of cancerogenesis is Hsp27. The presence of Hsp27 and anti-Hsp27 in the sera of patients with ovarian carcinoma is still a new research field, and papers contain contradictory res...
Article
The aim of this study was to evaluate the ferric-reducing ability of serum (FRAS), paraoxonase 1 (PON1), ceruloplasmin serum oxidase activity and hsCRP level in patients with type1 diabetes mellitus without and with diabetic retinopathy. The study was performed in 76 patients with type 1 diabetes mellitus, 35 without diabetic retinopathy (group 1)...
Article
Oxidized low-density lipoproteins (ox-LDL) have been shown to be strongly atherogenic and immunogenic in experimental animals and in humans. Autoantibodies against ox-LDL and its various epitopes have been detected in sera and in atherosclerotic lesions. LDL oxidation may also lead to generation of oxidized derivatives of cholesterol (eg, 7-ketocho...
Article
Kidney injury represents a major clinical problem in orthotopic heart transplant (OHT) patients which seriously increases the mortality rate. The aim of this work was to evaluate the utility of a new kidney damage marker-neutrophil gelatinase-associated lipocalin (NGAL)-and its correlations with cystatin C, creatinine, and glomerular filtration rat...
Article
The aim of this study was to present the usefulness of enzyme-linked immunosorbent assay (ELISA) in nicotine metabolites determination in urine and serum samples from active smokers and compare it with the reference to thin layer chromatography (TLC) with densitometry. The specific anti-cotinine antibodies were obtained from rabbit sera after seque...
Article
Disorders of lipid metabolism and antioxidant defense capacity reported during idiopathic nephrotic syndrome (INS) exacerbations are known. The aim of this study was to evaluate constituents of antioxidant defense [total antioxidant potential: ferric-reducing antioxidant power (FRAP), paraoxonase-1 (PON-1), tocopherols, ascorbic acid] in patients f...
Article
Full-text available
Ions of irons, especially ferrous ions may be harmful for living organisms, because they generate reactive oxygen species like O2•- or •OH. Probability of the risk rises especially in pathological conditions, in which high level of iron is observed. For this reason scientists try to establish new methods that can support organism in eliminating rea...
Article
The influence of vitamin and non-vitamin antioxidants upon lipid peroxidation free radical processes generated by administering sodium fluoride to rats has been examined by measuring the concentration of malondialdehyde (MDA) in rat blood plasma. Fifty-four female Wistar FL rats were divided into 9 groups of 6 rats each in a 35-day experiment. The...
Article
In this study, the effect of the raspberry seed extract (RSE) on the ferroxidase activity of ceruloplasmin (Cp) isolated from healthy male subject serum was analyzed. The ferroxidase activity of Cp was determined by spectrophotometry using Fe(II) – histidine complex and ferrozine as a chromogenic reagent. The ferroxidase activity of ceruloplasmin w...
Article
Full-text available
Unlabelled: The study population included employees of metal works, with significant exposure to lead (Pb) for about 20 years (mean blood lead level PbB = 43 microg/dl), divided into four groups: normotensive (Pb-normotensive), high-normotensive, first (HT-1), and second degree (HT-2) of hypertension. The control group comprised of 30 office worke...
Article
Full-text available
In this paper the physiological role of NO and isoforms of NOS in the gastrointestinal tract and the involvement of NO in pathological processes of digestive tract as well as the perspective of therapeutic use of NO-donating drugs and selective inhibitors of phosphodiesterase in the treatment of gastric diseases were presented.
Article
Exposure to tobacco smoke is an extremely important risk factor determining the development of respiratory diseases, cardiovascular diseases and cancer. Passive exposure is common and often not realized by the exposed subjects. Markers of tobacco smoke exposure are nicotine metabolites, i.e. cotinine and trans-3'-hydroxycotinine. The objective of t...
Article
Unlabelled: A total of 30 patients (ten female/20 male), 9 years to 22 years old (mean age 17.3 years) and 30 healthy teenage controls (mean age 16.4 years) were included in our study. The patients had steroid-sensitive idiopathic nephrotic syndrome (INS) and had completed steroid therapy 4 years to 15 years ago. Height and weight, body mass index...
Article
Full-text available
The aim of the study was to evaluate the effect of different degrees of chronic ischemia of the lower limbs on PON1 activity in plasma, in relation to different substrates. The studied group consisted of patients with chronic arterial occlusion of the lower limbs due to atherosclerosis. The paraoxonase and arylesterase activities of PON1 were measu...
Article
Interaction between cells via intimate cell-cell contact is facilitated by a cell surface molecules, termed adhesion molecules. The aim of the study was to evaluate the blood serum concentration of soluble forms of vascular cell adhesion molecule (VCAM-1), intercellular adhesion molecule (ICAM-1) and endothelial leukocyte adhesion molecule-1 (ELAM-...
Article
Aetiopathogenesis of psoriasis is complex and not yet well known. In recent years, it has been observed that psoriasis can coexist with clinically asymptomatic celiac disease and a gluten-free diet helps to obtain remission, even in patients with very chronic lesions. The aim of our work was to investigate how often the positive titres of antibodie...
Article
Full-text available
We present the case of a 9-year-old boy with DOOR syndrome recognized in the first year of his life because of a delayed development of speech. The diagnosis was based on characteristic abnormalities, including congenital deafness, nail and bone abnormalities, and mild mental retardation.
Article
Introduction. Psoriasis is a disease related to disorders of lipid metabolism. Modifications of lipid concentrations may increase risk of arteriosclerosis observed in psoriatic patients. Aim of the study. Assessment of the concentrations of selected lipids, oxidation markers and the efficiency of antioxidant defense in psoriatic patients with diffe...
Article
Pancreatic cancer constitutes a difficult diagnostic and therapeutic problem. It remains the fourth cause of death among gastrointestinal malignancies. The present study aimed to investigate whether neopterin can be used as a significant marker in pancreatic cancer and to establish any possible relationships between serum neopterin levels and the d...
Article
Full-text available
The aim of this study was to evaluate the blood concentration of hepatocyte growth factor (HGF) in patients at various stages of retinopathy. We hypothesised that the high level of HGF found in diabetic patients may be an important marker of retinopathy progression and that HGF level may be an index of the risk of proliferative retinopathy. The par...
Article
Among cardiac transplantation (OHT) of coronary arterial disease, the pathogenesis can be associated with autoimmunologic effects due to oxidative lipoprotein modification and their change in antigenicity. These factors may lead to lipoprotein vascular changes observed in antiphospholipid syndrome or systemic lupus erythematosus. The aim of the pre...
Article
The soluble forms of vascular cell adhesion molecule-1 (sVCAM-1) and intercellular adhesion molecule-1 (sICAM-1) have been found to be increased in the blood of patients with Graves disease. The aim of this study is evaluation of the serum concentrations of soluble forms of adhesion molecules ICAM-1 and VCAM-1 in patients with thyroid-associated or...