Tomasz Warzecha

• PhD
• Research Associate at University of Agriculture in Krakow

To be useful for cereal breeding, cytoplasmic male sterility (CMS) should express the complete sterility of maternal lines and the full restoration of the male fertility of F1 hybrids. The most reliable source of sterilizing cytoplasm for triticale is Triticum timopheevi; however, due to the low frequency of efficient non-restorer genotypes for thi...

As a result of oat (Avena sativa L.) × maize (Zea mays L.) crossing, maize chromosomes may not be completely eliminated at the early stages of embryogenesis, leading to the oat × maize addition (OMA) lines development. Introgression of maize chromosomes into oat genome can cause morphological and physiological modifications. The aim of the research...

The number of polyploid plants in angiosperms is estimated at about 30-80%, depending on the study, but hybridization between diploids and tetraploids is not a common phenomenon. In this study, our aim was to determine the extent of hybridization and introgression between Aconitum species in a mountainous hybrid zone in the Tatra Mountains (Western...

Plant growth and the process of yield formation in crops are moderated by surrounding conditions, as well as the interaction of the genetic background of plants and the environment. In the last two decades, significant climatic changes have been observed, generating unfavorable and harmful impacts on plant development. Drought stress can be conside...

The growth of plant and process of yield formation in crops are moderated by surrounding conditions as well as interaction of genetic background of plants and environment. In the last two decades significant changes in climate can be observed generating unfavorable and harmful impact on plant development. Drought stress can be considered as one of...

The ontogenesis and yield formation in crop plants are modified by environmental conditions. Due to climatic change detected over two decades, the harmful influence of abiotic factors is increasing. One of the most threatening issues reducing plant productivity is drought stress. The strength of plant response to water shortages could differ depend...

In our study, we focused on the link among various parameters of chlorophyll a fluorescence and yield elements in the barley doubled haploid (DH) lines. There were significant differences in all studied DH lines, both in yield components and parameters of chlorophyll a fluorescence. The most variable parameter was overall performance index of PSII...

The genetic diversity in 11 populations of Gladiolus imbricatus in five mountain ranges, including the Tatra, Pieniny, Gorce, Beskid Niski (Western Carpathians) and Bieszczady Mts (Eastern Carpathians), was studied with inter-simple sequence repeat (ISSR) markers. The species is a perennial plant occurring in open and semi-open sites of anthropogen...

HHybridization in Aconitum is a widespread phenomenon. However, the introgression between the diploid and tetraploid species is rare. We studied species of different ploidy in a mountain hybrid zone in the Tatra Mts (West Carpathians). In this paper, we aimed to identify the extent of introgressive hybridization using PCR-ISSR fingerprinting and ch...

The oat × maize chromosome addition (OMA) lines, as hybrids between C3 and C4 plants, can potentially help us understand the process of C4 photosynthesis. However, photosynthesis is often affected by adverse environmental conditions, including drought stress. Therefore, to assess the functioning of the photosynthetic apparatus in OMA lines under dr...

The nucleus architecture of hybrid crop plants is not a well-researched topic, yet it can have important implications for their genetic stability and usefulness in the successful expression of agronomically desired traits. In this work we studied the spatial distribution of introgressed maize chromatin in oat × maize addition lines with the number...

Barley worldwide is affected seriously by Fusarium seedling blight (FSB) and Fusarium head blight (FHB) diseases caused by the Fusarium species. The objective of this study was to facilitate the resistance of hulled and hull-less barley at different growth stages to F. culmorum according to direct parameters: disease rating (DR), fresh weight of le...

The objective of this study was to assess genotype by environment interaction for 1000-kernel weight in spring barley lines grown in South Poland by the additive main effects and multiplicative interaction model. The study comprised of 32 spring barley (Hordeum vulgare L.) genotypes (two parental genotypes—breeding line 1 N86 and doubled haploid (D...

Background The oat × maize addition (OMA) lines are used for mapping of the maize genome, the studies of centromere-specific histone (CENH3), gene expression, meiotic chromosome behavior and also for introducing maize C4 photosynthetic system to oat. The aim of our study was the identification and molecular-cytogenetic characterization of oat × mai...

Efficiency of oat haploid embryos and plants production using wide crossing with maize

Chlorophyll a fluorescence can provide insight into the ability of plants to tolerate environmental conditions that can damage photosynthetic apparatus and decrease yield. The aim of the study was to determine the relationship between chlorophyll a fluorescence parameters and yield components of oat DH lines. All DH lines significantly differed in...

The tetraploid (2n = 32) Aconitum sect. Aconitum in the Eastern Carpathians, Southern Carpathians and Apušeni Mts. is represented by high-mountain A. bucovinese, A. firmum subsp. fissurae and their putative taxonomic hybrid A. ×nanum. The aim of the paper was to reveal which delimiting system: Taxonomic vs. geographic-population better explains gen...

Hybridization among species of A conitum effects their morphology and ecology. In this study the hybridization between the diploid 2n(2x) = 16 ( A . lasiocarpum and A . variegatum) and tetraploid 2n(4x) = 32 ( A . firmum) species was documented in the Tatra Mountains (Western Carpathians) in a small, local population in a semi-natural site. The hyb...

Background Meiotic recombination is a major source of genetic variation in eukaryotes. The role of recombination in evolution is recognized but little is known about how evolutionary forces affect the recombination pathway itself. Although the recombination pathway is fundamentally conserved across different species, genetic variation in recombinat...

Obtaining oat DH lines is only effective via wide crossing with maize. Seven hundred haploid embryos from 21 single F1 progeny obtained from wide crosses with maize were isolated, divided into four groups according to their size (<0.5 mm, 0.5–0.9 mm, 1.0–1.4 mm, and ≥1.5 mm), and transferred into 190–2 regeneration medium with different growth regu...

Triticale possesses a number of attributes that make it amenable for development into a hybrid cereal breeding system. It possesses a tendency for outcrossing, a large percentage of open flowers, longer duration of flowering, a high percentage of anther extrusion including high amount of pollen shed, and a longer pollen lifetime in comparison with...

The co-occurrence of hybrids and parental species in similar ecological niches poses a question on the role of traits additivity and overdispersion (emergence of new traits) in microevolutionary processes. We analysed genetic polymorphism of Bromus benekenii, B. ramosus and the spontaneous hybrid B. benekenii × B. ramosus in sympatric and allopatri...

Abstract The Triticum timopheevi cytoplasmic male sterility (cms) system in triticale (×Triticosecale Wittmack) suffers from a low frequency of maintainers and environmental instability of the male sterility. On the other hand, the Pampa cms system in rye (Secale cereale) exhibits strong male sterility and a low frequency of restorers. Here, we rep...

Despite not having been fully recognized, the cryptic northern refugia of temperate forest vegetation in Central and Western Europe are one of the most important in the Holocene history of the vegetation on the subconti-nent. We have studied a forest grass Bromus benekenii in 39 populations in Central, Western and Southern Europe with the use of PC...

This paper reports a continuation of our previous research on the phytochelatin synthase1 (PCS1) gene involved in binding and sequestration of heavy metals or metalloids in plant cells [1]. Construction of a 3D structure of the Arabidopsis thaliana PCS1 protein and prediction of gene function by employing iterative implementation of the threading a...

We studied the thermophilous grass Bromus erectus in Central Europe to determine its pattern of population genetic structure and genetic diversity, using ISSR-PCR fingerprinting to analyze 200 individuals from 37 populations. We found three genetic groups with a clear geographic structure, based on a Bayesian approach. The first group occurred west...

Previously, our in silico analyses identified four candidate genes that might be involved in uptake and/or accumulation of arsenics in plants: arsenate reductase 2 (ACR2), phytochelatin synthase 1 (PCS1) and two multi-drug resistant proteins (MRP1 and MRP2) [Lund et al. (2010) J Biol Syst 18:223-224]. We also postulated that one of these four genes...

Vigor and selected physiological parameters (content of phenolic compounds, soluble sugars, chlorophyll a and b, and carotenoids) of eight naked and two husked oat cultivars harvested at 15% moisture content were determined. Oat seeds were threshed using two rotational speeds of the threshing drum: 1.6 m s−1 (LS) and 2.4 m s−1 (HS). They were then...

Infection of plants by pathogens is a biotic environmental stress. Barley plants are infected, among others, by Fusarium culmorum—a pathogen affecting seedling, head, root and stem. The infection can result in reduced yield and grain quality. The aim of the study was to compare the reaction of inoculated and non-inoculated barley doubled haploids (...

Previously, we have reported a T-DNA tagged mutant (TAG_009) of Arabidopsis thaliana exhibiting a significant sensitivity to biotic stresses. We have also cloned and analyzed the tagged gene At5g46050. Based on bioinformatic and molecular characterization, we proposed that At5g46050 is involved in the transport of peptides participating in plant de...

Fusarium culmorum (W.G.Sm.) Sacc. is a pathogen affecting seedling, head, root and stem of barley, and the infection can result in significantly reduction of yield and grain quality. The aim of the studies was to compare the susceptibility of hulless and hulled barley doubled haploids (DH) to infection with F. culmorum. Thirty DH lines (15 hulled a...

Celem badań było określenie zróżnicowania podatności na fuzaryjną zgorzel siewek 12 odmian pszenżyta ozimego oraz 6 odmian pszenicy ozimej pochodzących z Hodowli Roślin DANKO. Ziarniaki inokulowano Fusarium culmorum, izolatem IPO348-01 o średniej patogeniczności, pochodzącym z Instytutu Ochrony Roślin w Wageningen, w Holandii. Dokonano oceny stopni...

Badano podatność 4 męskosterylnych linii pszenżyta, 5 rodów i odmian ojcowskich oraz 20 mieszańców F1 otrzymanych z krzyżowania tych form w układzie czynnikowym, na zgorzel siewek wywołaną przez Fusarium culmorum. Ziarniaki inokulowano izolatem IPO348-01 pochodzącym z Instytutu Ochrony Roślin w Wageningen w Holandii. Pojedyncze izolaty F. culmorum...

Badano podatność izogenicznych linii pszenżyta, różniących się rodzajem cytoplazmy i męską płodnością (4 linie męskosterylne z cytoplazmą T. timopheevi i 4 linie dopełniające z cytoplazmą T. aestivum) na zgorzel siewek wywołaną przez Fusarium culmorum. Ziarniaki inokulowano Fusarium culmorum, izolatem IPO348-01 pochodzącym z Instytutu Ochrony Rośli...