Tomasz Podgorniak

Tomasz Podgorniak
Norwegian University of Life Sciences (NMBU)

PhD

About

15
Publications
2,502
Reads
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143
Citations
Introduction
AQUA-FAANG project: optimization of chromatin analysis in different species of fish and tissues
Additional affiliations
November 2012 - February 2016

Publications

Publications (15)
Article
Animal domestication is a process of environmental modulation and artificial selection leading to permanent phenotypic modifications. Recent studies showed that phenotypic changes occur very early in domestication, i.e., within the first generation in captivity, which raises the hypothesis that epigenetic mechanisms may play a critical role on the...
Article
Full-text available
In vertebrates, the somatotropic axis comprising the pituitary gland, liver and muscle plays a major role in myogenesis. Its output in terms of muscle growth is highly affected by nutritional and environmental cues, and thus likely epigenetically regulated. Hydroxymethylation is emerging as a DNA modification that modulates gene expression but a ho...
Article
Full-text available
Growth is a complex trait whose variability within a population cannot be explained solely by genetic variation. Epigenetic regulation is often suggested as an important factor shaping the phenotype, but its association with growth can be highly context- and species-dependent. Nevertheless, the mechanisms involved in epigenetic regulation of growth...
Article
Full-text available
L’anguille européenne Anguilla anguilla est une espèce catadrome avec un cycle de vie complexe incluant des migrations entre la mer de Sargasses, l’Europe et l’Afrique du Nord. Du fait de sa baisse drastique de population depuis 30 ans, l’anguille est considérée aujourd’hui comme « en danger critique d’extinction ». Une des causes de ce déclin est...
Article
Full-text available
Fishways are built on obstacles to restore the connectivity between aquatic habitats. Our study investigated how species-specific fishways interact with interindividual trait differences among fish. We compared several groups of glass eels (Anguilla anguilla) climbing two types of fishways with those remaining below the water impoundments. We analy...
Thesis
The European eel Anguilla anguilla is a catadromous fish with a large scale migration loop including the Sargasso sea, Europe and North Africa. In the last 30 years, drastic declines of abundance have been observed and the species is currently considered as critically endangered. One of the main causes of species decline is habitat fragmentation, w...
Article
Full-text available
European eel (Anguilla anguilla) is a catadromous fish species that received substantial attention as its population has markedly declined in the last three decades. The possible causes of this decline include habitat fragmentation factors such as dams and weirs. In some cases, these obstacles are equipped with fish friendly passage devices that ma...
Thesis
(trad auto)Anguilla anguilla anguilla est un poisson catadrome qui migre en boucle à grande échelle entre la frayère tropicale de la mer des Sargasses et les habitats de croissance continentaux de la zone européenne et nord-africaine (Tesch 2003). Au cours des 30 dernières années, on a observé des baisses drastiques de l'abondance. L'anguille europ...
Article
Full-text available
European eel is a panmictic species, whose decline has been recorded since the last 20 years. Among human-induced environmental factors of decline, the impact of water dams during species migration is questioned. The main issue of this study was to pinpoint phenotypic traits that predisposed glass eels to successful passage by water barriers. The a...
Poster
Full-text available
Physical obstacles to upstream migration such as dams are a major impairment of natural colonisation and dispersion of eels. Dams and obstacles increase the energy demand of fish, even when they are equipped with fish friendly devices. Besides, local accumulation of eels below dams increase the mortality associated with predation. Obstacles may als...
Article
Full-text available
Human activities have resulted in the decline of native crayfish and promoted the spread of invasive crayfish species in European fresh waters, threatening ecosystem structure and functioning. We compared effects of native noble crayfish (Astacus astacus), invasive signal crayfish (Pacifastacus leniusculus) and of the absence of crayfish on leaf li...
Article
Full-text available
Selectivity of recreational angling on fish behaviour was studied by examining whether capture order or lure type (natural v. artificial bait) in ice-fishing could explain behavioural variation among perch Perca fluviatilis individuals. It was also tested if individually assessed personality predicts fish behaviour in groups, in the presence of nat...
Conference Paper
Physical obstacles to upstream migration such as dams are a major impairment of natural colonisation and dispersion of eels. Dams and obstacles also increase the energy demand of fish, even if there is a fish friendly device to allow them to swim across the obstacle. Besides, local accumulation of eels below dams may increase the mortality as...

Questions

Question (1)
Question
Hi, I am running a ChIPseq protocol. After the IP and decrosslinking step, I add PB buffer from MinElute kit. I always need to adjust the pH with sodium acetate ( 3M, pH =5.0) to bring back the color of the PB-DNA mixture to yellow ( <7.5 pH). However, it is not the case of my input. I suppose the alkaline conditions are due to my ChIP elution buffer ( 1% SDS and 100mM sodium bicarbonate) and I am getting lower % recovery of IPed DNA. I am wondering if all of you (using the column purification kits) need to adjust the pH at that step? What is the pH of your ChIP elution buffer? Nota: I prefer to use column purification over phenol:chloroform method due to its rapidity and lower variation in performance between the hands of the users. Thanks!

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Projects

Projects (3)
Project
​The European project AQUA-FAANG aims to improve understanding the genome function and usage of genotype-to-phenotype prediction in the six most important European farmed fish species. ​ AQUA-FAANG brings together world-leading interdisciplinary expertise and industry partners providing direct pathways to commercial exploitation.
Project
Domestication- related epigenetic changes in fish.