Tomasz Baj

Medical University of Lublin · Department of Pharmacognosy with Medicinal Plants Garden

Mixture design is a statistical tool used to obtain the maximum desired effect using the minimum number of experiments. The aim of the presented work was the optimization of the composition of a mixture of essential oils from basil, citronella, cedarwood and thyme using simplex-lattice mixture design method. The optimized parameter was an antioxida...

This study aimed to identify the chemical composition of the Spondias tuberosa aqueous leaf and root extracts (EALST and EARST) and to evaluate their effect, comparatively, against opportunis-tic pathogenic fungi. Ultra-Performance Liquid Chromatography Coupled to a Quadrupole/Time of Flight System (UPLC-MS-ESI-QTOF) was employed for chemical analy...

The occurrence of candidiasis, including superficial infections, has recently increased dramatically, especially in immunocompromised patients. Their treatment is often ineffective due to the resistance of yeasts to antimycotics. Therefore, there is a need to search for new antifungals. The aim of this study was to determine the antifungal effect o...

Yeasts from the Candida parapsilosis complex are clinically relevant due to their high virulence and pathogenicity potential, such as adherence to epithelial cells and emission of filamentous structures, as well as their low susceptibility to antifungals. D-limonene, a natural compound, emerges as a promising alternative with previously described a...

Commercialized mouthwashes are generally expensive for the most financially vulnerable populations. Thus, several studies evaluate the antimicrobial potential of herbal products, such as essential oils, to reduce the activity of microorganisms in the mouth. The objective of this research was to carry out the chemical characterization and antibacter...

The composition of the ethanolic extract from the aerial parts of Crocus alatavicus Regel & Semen from southern Kazakhstan spontaneous flora was analyzed together with the determination of its antibacterial, antifungal, antiviral and anticancer activity. The phytochemical profile analysis by high-performance liquid chromatography-electrospray ioniz...

This article reviews the available research results of selected species of the genus Silphium L. (Asteraceae) as alternative plants for crops and industry. Silphium species have valuable qualities across a wide range of uses, which is very important in considering plant resources as a green alternative to a sustainable future. Species of the genus...

Sage is a common ingredient in herbal mixtures and is also found in the form of infusion bags. Due to its culinary qualities, it is also grown in gardens as a spice. In the present work, an analysis of the composition of essential oil from sage obtained from commercial products and our three-year cultivation was carried out. Hydro-distillation of e...

Cancer diseases remain major health problems in the world despite significant developments in diagnostic methods and medications. Many of the conventional therapies, however, have limitations due to multidrug resistance or severe side effects. Bladder cancer is a complex disorder, and can be classified according to its diverse genetic backgrounds a...

A comprehensive approach to the mineral composition of black teas of different origins was studied using the Flame Atomic Absorption Spectrometry (FAAS) method, supported by chemometric tools including Principal Component Analysis PCA) and Classification and Regression Trees (CART). Significant differences between the teas from seven countries (Jap...

Background Yarrowia lipolytica is an oleaginous yeast with the ability to grow in a variety of hydrophilic and hydrophobic substrates, including industrial wastes, in which it produces and accumulates various nutrients. Methods The aim of the present study was to examine the presence of free L-carnitine in the biomasses of two Yarrowia lipolytica...

Background Yarrowia lipolytica is oleaginous yeast with the ability to grow in a variety of hydrophilic and hydrophobic substrates, including industrial wastes, in which it produces and accumulates various nutrients.Methods The aim of the present study was to examine the presence of free L-carnitine in the biomasses of two Yarrowia lipolytica strai...

The chemical composition of the hydroethanolic extracts (60% v/v) from the aerial parts of Thymus marschallianus Willd (TM) and Thymus seravschanicus Klokov (TS) from Southern Kazakhstan flora was analyzed together with their hexane fractions. Determination of antibacterial, antifungal and antioxidant activities of both extracts was also performed....

Current trends in the industry indicate that extraction solvents should conform with the ideals of so-called “green chemistry”. Therefore, the objective of the presented study was to optimize the conditions for the extraction of polyphenols, flavonoids and chlorophyll from peppermint leaves (Mentha piperita L., Lamiaceae) and from common nettle lea...

Yarrowia lipolytica as an oleaginous yeast is capable of growing in various non-conventional hydrophobic substrate types, especially industrial wastes. In this study, the content of thiamine (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B6), biotin (vitamin B7) and folic acid (vitamin B9) in the wet biomass of Y. lipolytica strains cul...

Lemongrass (Cymbopogon citratus (DC) Stapf.) is a perennial plant indigenous to semi-tropical regions of Asia and cultivated in other semi-tropical countries. The present study aimed to examine the key chemical constituents of various parts of lemongrass cultivated in the temperate climate of Poland. The content of essential oil and its composition...

Extracts from the June collection of aerial parts of Helianthus salicifolius A. Dietr and Helianthus tuberosus L. were obtained using carbon dioxide supercritical fluid extraction with water as co-solvent. The antimicrobial effect in vitro of these extracts was then determined against reference species of bacteria, as well as against fungi (represe...

The extracts from the aerial parts of Helianthus salicifolius A. Dietr and Helianthus tuberosus L. collected in June were obtained using carbon dioxide supercritical fluid extraction with water as co-solvent. The antimicrobial activity in vitro of these extracts were determined against the reference species of Gram-positive and Gram-negative bacter...

[This corrects the article DOI: 10.1155/2019/5465463.].

The purpose of this research was to investigate the chemical composition of essential oils (EOs) from: Origanum vulgare L., Satureja hortensis L., Thymus serpyllum L. and Thymus vulgaris L. (Lamiaceae) cultivated in Poland, and to study their antifungal activity towards clinical isolates of oral Candida spp. The hydrodistilled essential oils were a...

Abstract Yarrowia lipolytica is an oleaginous yeast species with the ability to grow on a number of substrates types, especially industrial wastes. This paper concerns the statistical optimization of fermentation parameters and media to ensure consistent and improved Y. lipolytica protein production. A strain of Y. lipolytica A-101 was observed to...

The anti-H. pylori properties of 26 different commercial essential oils were examined in vitro by MIC (minimal inhibitory concentration) determination for the reference strain H. pylori ATCC 43504. We selected 9 essential oils with different anti-Helicobacter activities and established their phytochemical composition and urease inhibition activitie...

An optimisation of extraction towards an increased antioxidant capacity and the study on the extracts' composition by HPLC-ESI-Q-TOF-MS were performed on different organs of a rarely studied plant: Rheum cordatum Losinsk (Polygonaceae) growing in Kazakhstan. More than 20 compounds from anthraquinones and phenolics were identified in an optimised me...

Background: Extracts from medicinal plants with phytochemicals with known antimicrobial properties can be an effective adjunct in the complex treatment of infectious diseases. This study aimed to evaluate the antimicrobial activity of wormwood extracts collected in Kazakhstan (Artemisia gmelinii Weber ex Stechm.), along with their phytochemical an...

The aim of this study was to investigate polyphenolic composition of different parts (leaves, flowers and fruits) of Crataegus almaatensis Pojark, an endemic plant of Kazakhstan, and compare it to a well known European Crataegus oxyacantha L. flowers. A Qual-Quant analysis was performed based on HR-MS measurements on 22 secondary metabolites: flavo...

In the concept of the aesthetic formation of knowledge and its as soon as possible and success-oriented application, insights and profits without the reference to the arguments developed around 1900. The main investigation also includes the period between the entry into force and the presentation in its current version. Their function as part of th...

Compounds with potent antioxidant properties are gaining interest among food manufacturers and consumers alike. With rising concerns about the safety of synthetic substances being added to food products, companies are beginning to search for natural sources of antioxidants. Because essential oils have well studied antioxidant properties they can be...

The total phenolic content (TPC), total tannin content (TTC) and total flavonoid content (TFC) as well as the antioxidant activity and the cytotoxic effect of the extract from leaves of Erythrochiton brasiliensis Nees & Mart. (Rutaceae) were evaluated. Raw material was collected in different European botanical gardens. Statistical analysis revealed...

Essential oils obtained from Origanum species are characterized by high diversity in composition. In this work, thin-layer chromatography (TLC)—fingerprinting of Origanum species was performed for the first time and enabled the discrimination of three chemotypes (carvacrol, caryophyllene oxide, and terpineol/sabinyl). Gas chromatography—mass spectr...

The purpose of the study was to analyze the chemical composition of the essential oil (EO) obtained from the Tilia cordata flowers from collected (“W”) and two commercially available products (“F” and “K”). Hydro-distillation of essential oils was carried out according to the Polish Pharmacopoeia VIII. Qualitative and quantitative analysis of the e...

The dietary supplements with claimed antioxidant activity constitute a substantial part of the dietary supplement market. In this study, we performed the LC-QTOF-MS analysis and investigated the activity profiles of popular antioxidant dietary supplements from different chemical groups in terms of quality control. The commonly used antioxidant test...

Satisfactory separation of a test mixture of 19 dyes with a general elution problem was obtained by reversed-phase stepwise gradient thin-layer chromatography with single void volume of the mobile phase. An effect of the supplementary mobile phase ffux to the surface of the adsorbent layer, observed previously, was eliminated. This improves the ffo...

The rare coumarins stenocarpin, stenocarpin isobutyrate, oficinalin, oficinalin isobutyrate, 8-methoxypeucedanin and the known xanthotoxin, isoimperatorin, bergapten, peucedanin and 8–methoxyisoimperatorin were isolated from Peucedanum luxurians Tamamsch. (Apiaceae) and identified by means of spectral data (1D and 2D NMR). Their immunomodulating ac...

Table S1. Antioxidant activity of dietary supplements. Table S2. The chemical composition of investigated dietary supplements.

The aim of this work was to develop a cream formulation with potential antioxidant properties. Herein, a freeze-dried extract of Ligusticum mutellina was used as a source of active compounds. The proposed qualitative composition of the cream was characterized by a good polyphenolic compounds release profile. Of note, the highest R 2 adj values were...

Tannins are an important group of secondary plant metabolites that were originally used in the leather production industry in the tanning of animal hides. The varied chemical structures and stability evidenced among the tannin group result in their classification as hydrolyzable, complex, and proanthocyanidins. This chapter outlines the copious ran...

Vitamins are chemical compounds necessary for homeostasis, as their absence contribute to various diseases. The recommended intake of vitamins is dependent on age and sex. The intake levels for pregnant and nursing mothers also vary. It is also important to note that interaction between vitamins and other compounds can result in overdose. Fruits, v...

Over the past five years, phytochemical and pharmacological studies have been conducted on material extracted from members of the Rutaceae family. In such work, new furochinoline-structured alkaloids were isolated from Ruta sp. and Dictamnus sp. Beyond the aforementioned, other substances with promising activity were isolated from the less-known sp...

In this study, both qualitative and quantitative analyses of chemical composition of M. purpurea essential oil obtained in the Deryng and Clevenger-type apparatuses were compared. As a result, content of volatile compounds were: 785.67 mg/mL and 833.33 mg/mL in the oil obtained in the Deryng (D-EO) and Clevenger-type apparatuses (C-EO), respectivel...

The aim of the study was to estimate potential availability of essential oil in some brands of herbal products. A comparison was performed on the basis of the essential oil yield in the unprocessed raw materials such as leaves of peppermint and lemon balm and inflorescence of chamomile as well as herbal tea bags and in dietary supplements. The yiel...

Astragalus mongholicus, commonly used in Traditional Chinese Medicine (TCM), contains significant amounts of triterpene saponins – astragalosides, which possess a wide variety of pharmacological actions, including the treatment of cardiovascular and respiratory diseases. The present study was performed to develop a fast and effective extraction met...

The aim of this study was to examine the usefulness of a pH-dependent copolymer - Eudragit FS - for employment in the technology of preparing modified release metronidazole matrix tablets. In addition, in our work, Eudragit RL and Eudragit RS were included in the composition of some formulations, as well as sodium lauryl sulfate and polysorbate 80....

Recently, several studies have been undertaken so as to develop more effective therapeutic approaches towards eradicating Helicobacter pylori. Among these is phytotherapy. The aim of this study was to investigate the activity in vitro of the plant extracts obtained from common herbs cultivated in the Lubelszczyzna region against the reference strai...

The determination of phenolic acids’ and flavonoids’ content in daily food rations (DFRs) of a selected group of male and female students and the development of chromatographic conditions is the primary goal of the study. The presence of 7 phenolic components were confirmed in the prepared extracts from all diets reconstructed within a period of 3...

Many phytochemical investigations have been focused recently on the anti-oxidant activity of herbal extracts which can be used in phytotherapy. The previous study revealed antioxidative properties of Mutellina purpurea extract, but the constituents responsible for this action were not described yet. The aim of this study was activity-guided separat...

This new book will cover a broad range of polymeric materials and textiles and provides industry professionals and researchers in polymer science and technology with a single, comprehensive book summarizing all aspects involved in the functional materials production chain. This volume presents the latest developments and trends in advanced poly...

Abstract: Mutellina purpurea L. belongs to Apiaceae family and is known as Alpin lovage considered as a forage for animals nutrition because of the presence of sufficient concentration of minerals like calcium and potassium. The chemical composition of the essential oil obtained by hydrodistillation in a Clevenger apparatus and aroma of inflorescen...

Abstract The delayed cardiomyopathy caused by doxorubicin - an chemotherapeutic drug with broad spectrum of anticancer activity - is mainly triggered by oxidative stress. The aim of this study was to assess an effect of Mutellina purpurea methanolic extract fraction and other antioxidants of plant origin: rutin, quercetin and chlorogenic acid (all...

In this paper, comparison of the volatile components composition in the samples obtained by hydrodistillation and solid-phase microextraction of Salvia officinalis was described. Different sample preparation techniques showed considerable differences in volatiles composition, especially with respect to sesqui- and diterpenoids. The comparison of th...

The chemical composition of the essential oils obtained from inflorescences, the root and herb of Ligusticum mutellina of Polish origin was studied and compared to plants collected from other locations. Analyses of L. mutellina essential oils of Polish origin revealed α-pinene as a predominant compound in the root (49.49%) and herb (9.76%), while s...

Xanthium strumarium (common cocklebur) pollen grains are included in allergenic types. During a three-year study (2003-2005) conducted by using the gravimetric method at two trap sites in Lublin, daily concentrations, maximum concentrations and annual sums of pollen grains, as well as the length of pollen seasons of this species were compared. The...

Mutellina purpurea is an aromatic Apiaceae plant known as Alpine lovage. Its polar extracts consist of phenolic acids, tannins and flavonoids. The cytotoxic effect of methanolic and aqueous extracts from M. purpurea was studied on the most frequently used cell lines: HeLa and BHK-21. Taking into account that the natural products are often used with...

After reviewing the sources of hazards during com. plastics processing (granulation of polyolefins, prodn. of shoes by processing PVC, thermoplastic rubber and polyurethane foams, prodn. of brushes by processing polyethyleneterephthalate, thermal processing of nitrile rubber, prodn. oflaminates by using styrene-contg. polyster resins), some hazardo...