Tomas Ocelka

• Ph.D
• Principal Investigator at E&H services, Inc.

The study was designed to assess the pollution of the Svitava and Svratka rivers in and around the industrial city of Brno (Czech Republic) by persistent organic pollutants using selected biochemical markers in chub. Levels of selected biochemical markers were measured in liver and plasma samples of chub. The concentrations of persistent organic po...

The aim of the present study is to assess aquatic ecosystem contamination using selected biochemical markers: cytochrome P450, ethoxyresorufin-O-deethylase (EROD), glutathione S-transferase (GST), tripeptide glutathione, vitellogenin, and 11-ketotestosterone in chub (Leuciscus cephalus L.). Seven locations on the Svitava and Svratka rivers (in the...

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The aim of this study was the assessment of the Svitava and Svratka rivers contamination by polycyclic aromatic hydrocarbons (PAH) using 1-hydroxypyrene (1-OHP) concentrations in fish bile as a biomarker. Levels of 1-OHP were determined by reverse phase HPLC with fluorescence detection. For valid assessment of bile accumulation levels, the 1-OHP co...

The values of thermochemical quantities (enthalpy and Gibbs energy of formation, entropy and molar heat capacity) in the ideal gas state and dihedral angles for all 209 polychlorinated biphenyls were obtained by the Benson contribution method and ab initio calculations. The di-, tri- and tetra-ortho-chlorinated biphenyls are related with properties...

The quality of chemical analysis is an important aspect of passive sampling-based environmental assessments. The present study reports on a proficiency testing program for the chemical analysis of hydrophobic organic compounds in silicone and low-density polyethylene (LDPE) passive samplers and hydrophilic compounds in polar organic chemical integr...

Non-psychotropic cannabinoids (e.g., cannabidiol, cannabinol and cannabigerol) are contained in numerous alimentary and medicinal products. Therefore, predicting and studying their possible side effects, such as changes in DNA methylation, is an important task for assessing the safety of these products. Interference with TET enzymes by chelating fe...

Flame retardants, i.e., chemical compounds added to products to increase fire safety, occur in all components of the environment. To perform an efficient mapping of their sources and ways of exposure of human body to them, it is necessary to know their concentration in the air, in solid materials, and waters. For this purpose, primarily, it is desi...

The paper describes the occurrence, possible routes of the exposure and analyses of the flame retardants in various components of the environment, focusing on the most frequently occurring types based on brominated and organophosphate compounds and mapping their presence in the country. Czech Republic is committed to do the monitoring within the fr...

An inter-laboratory study was organised for the monitoring of emerging aquatic pollutants (pharmaceuticals, pesticides, steroids, brominated diphenyl ethers and others) using passive samplers. Thirty laboratories participated in the sampler comparison exercise. Various samplers designs were exposed at a single sampling site to treated waste water....

This study investigated polycyclic aromatic hydrocarbons (PAH) pollution of selected rivers in the Czech Republic. Integrated evaluation was carried out using combination of chemical and biological monitoring, in which we measured content of 1-hydroxypyrene (1-OHP) in chub bile and priority PAH in water samples obtained by exposing the semipermeabl...

The following provides a short overview of the important topics arising from the 6(th) International Passive Sampling Workshop and Symposium (IPSW 2013) held in Bordeaux, France between 26 and 29(th) June, 2013. Most of the discussions focussed on monitoring non-polar and polar organic pollutants in water with less coverage on air (probably already...

Backround. Deep skin burn injuries, especially those on the face, hands, feet, genitalia and perineum represent significant therapeutic challenges. Autologous dermo-epidermal skin grafts (DESG) have become standard of care for treating deep burns. Additionally, human autologous thrombin activated autologous platelet concentrate (APC) has gained acc...

Passive and composite sampling in combination with in vitro bioassays and identification and quantification of individual chemicals were applied to characterize pollution by compounds with several specific modes of action in urban area in the basin of two rivers, with 400,000 inhabitants and a variety of industrial activities. Two types of passive...

Sulfonamide antibiotics coming from both human and veterinary medicine are among the most common emerging pollutants in freshwater. The present paper shows the successful application of passive sampling using POCIS in combination with an immunochemical ELISA technique and HPLC/MS/MS analysis to study the distribution of sulfonamides in streams arou...

Solid-fuel combustion in small heating appliances produces notable quantities of pollutants. Emission levels of pollutants are estimated using emission factors and fuel consumption data. Emission factors for small heating appliances depend, among others, on the fuel type, appliance design, fuel loading and operation conditions. At present, it is ne...

Passive sampling devices have been used since the 1970s to measure time-weighted average (TWA) or equilibrium concentrations of pollutants in various environmental matrices (e.g. air, soils and sediments and water). In recent years the popularity of using such samplers has increased and the technology in now well established for the measurement of...

This study reports on the first complex data set of emission factors (EFs) of selected pollutants from combustion of five fuel types (lignite, bituminous coal, spruce, beech, and maize) in six different domestic heating appliances of various combustion designs. The effect of fuel as well as the effect of boiler type was studied. In total, 46 combus...

Polar organic compound integrative samplers (POCIS) in combination with instrumental techniques such as LC-MS-MS were previously used to monitor environmental pollutants but the performance of alternative immunochemical methods such as ELISA (enzyme-linked immunosorbent assay) has been explored less. In the present study, POCIS technology was appli...

Endocrine-disrupting chemicals (EDC) are manmade or natural chemicals that have the ability to interfere with the endocrine system of animals. They have not been monitored systematically in the Czech Republic. The goal of the present study was the characterization of aquatic environmental pollution from the Brno (Czech Republic) city agglomeration...

267. T. Ocelka, M. Meloun, L. Pavliska: Monitoring a statistické hodnocení dat z aplikace pasivních vzorkovačů – Interpretace výsledků z aplikace pasivních vzorkovačů, Sborník přednášek ze semináře „Zajištění kvality analytických výsledků“ dne 24.3. – 26.3.2010 v Komorní Lhotce, strana 21 - 22, Vydává 2THETA, P.S. 103, 737 01 Český Těšín, ISBN 978-...

The aim of the present study was to assess aquatic contamination by polycyclic aromatic hydrocarbons (PAH), using the 1-hydroxypyrene (1-OHP) content in fish bile as a biochemical marker. A total of 71 chub (Leuciscus cephalus L.) were collected from seven locations on the Svitava and Svratka rivers in and around the industrial city of Brno, Czech...

The Stockholm Convention on Persistent Organic Pollutants (POPs) defines as one of its aims the application of technologies to minimize environmental threats posed by POPs. In Article 6 of the convention there are performance standards stated for the use of best available technologies (BAT) to dispose of stockpiles of PCBs and other POPs. The top p...

The Stockholm Convention on Persistent Organic Pollutants (POPs) defines as one of its aims the application of technologies to minimize environmental threats posed by POPs. In Article 6 of the convention there are performance standards stated for the use of best available technologies (BAT) to dispose of stockpiles of PCBs and other POPs. The top p...

The Stockholm Convention on Persistent Organic Pollutants (POPs) defines as one of its aims the application of technologies to minimize environmental threats posed by POPs. In Article 6 of the convention there are performance standards stated for the use of best available technologies (BAT) to dispose of stockpiles of PCBs and other POPs. The top p...

A 6-month air sampling survey was conducted at 26 sites across the African continent with the aim to establish baseline information on contamination of ambient air with persistent organic pollutants (POPs) as a reference for future monitoring programs in the region. Sampling sites included continental, rural and urban backgrounds, agricultural and...

Persistent compounds are present around almost the entire world. The level of contamination in very old groundwater sources (Cennoman bedrock Mesozoic, approximately 100 millions year old) was assessed. This offers an information about realistic natural background. Together with chemical analysis a toxicity evaluation of sampled sites was performed...

The article is focused on dioxin, furan, PCB and organochlorine pesticide monitoring in the surface waters of the Central European, protected natural reserve Krivoklatsko, under the UNESCO programme Man and Biosphere. Persistent compounds are presently transported via different means throughout the entire world. This contamination varies significan...

Polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) belong to group persistent toxic substances emitted from various thermal processes. Recently, main interest was focused on greatest sources of PCDD/F, like incinerators, metallurgic plants, etc…. However, latest concern is also focused on small burning facilities for domestic purposes th...

The most important features of the CMD (copper mediated dehalogenation) method are presented. The experimental dechlorination pathways of decachlorobiphenyl are outlined and compared with thermodynamic calculations. The dechlorination pathways of some more important precursors such are polychlorinated benzenes and phenols are discussed as well. The...

Polychlorinated biphenyls (PCBs) were used in Czech Republic (former Czechoslovakia), dominantly in the form of technical mixtures called Delor, Hydelor and Deloterm. Officially, over 10 kt was used inside Czechoslovakia, produced in eastern Slovakia (chemical plant Chemko - Strazske) from 1959 to 1984. Due to wide use of PCBs in open and closed sy...

Stockholmsk £mluva o persistentn¡ch organickìch polutantech je glob ln¡ environment ln¡ smlouvou, jej¡m§ c¡lem je ochrana lidsk‚ho zdrav¡ a §ivotn¡ho prostýed¡ pýed çkodlivìmi vlivy persistentn¡ch organickìch polutant… (d le jen "POPs"). émluva byla sjedn na v kvØtnu 2001 na Konferenci zplnomocnØnìch z stupc…, svolan‚ k tomuto £Ÿelu vìkonnìm ýedite...

Basic ideas of the mathematical gnostics are briefly described as a methodology comprising: 1) gnostic theory of individual uncertain data and of their samples, 2) methods of data analysis based on this theory, 3) programs realizing these methods, 4) examples of applications of the methods. KEYWORDS data analysis, gnostic approach, statistical appr...

Basic ideas of the mathematical gnostics are briefly described as a methodology comprising: 1) gnostic theory of individual uncertain data and of their samples, 2) methods of data analysis based on this theory, 3) programs realizing these methods, 4) examples of applications of the methods.

This work evaluates the toxicity of waters originated from the motorways and compares the concentrations of chosen pollutants with existing limits for waters and sediments.Water washed away from road surface and drainage water from the layers under the roads are conducted to small settlers and retention ponds. Pooled samples of water and sediments...

The efficiency of treatment processes forboth municipal and industrial wastewater (treatment plant-Ostrava, Czech Republic) focused on persistent organic pollutants (POPs) was assessed. Semipermeable membrane devices (SPMDs) as a sampling system were applied. Exposed SPMDs were analyzed both for chemical contaminants of POPs and toxicity response....

The present paper concentrates on the problem of monitoring of organic pollutants which even in trace level concentration can affect the algal biota. Such substances are difficult to analyse in the environment via common point sampling methodology followed by any analytical procedure. The conventional analytical methods of analysis of persistent or...

In former decades various persistent organic substances were produced and thus released into surrounded environment in Czech Republic. This work describes an application of passive sampling with semipermeable membrane devices (SPMDs) used for chemical and ecotoxicological monitoring in the area of by UNESCO protected area Křivoklátsko (Man and Biod...

The semipermeable membrane devices (SPMDs) have already been used in many environmental applications worldwide so far. For presented application, conventional, standard SPMDs have been applied to monitor the presence and concentrations of priority persistent organic pollutants (POPs) in river basins in the Czech Republic. This work covers applicati...

The sampling is generally known as main source of measurement errors. It is necessary to evaluate its uncertainty for consequent data handling. Parallel sampling and analysis of membranes exposed in the same sampler were included to QA/QC of SPMD measurements. These experiments were performed in different water systems ľ rivers, wastewater and glac...

Results of an experimental examinations are the more applicable in practice the more rigorous objectivity principles are respected. The true reality is reflected by data which ordinarily suffer of a portion of uncertainty. The data analysis applied to estimate the true data values masked by uncertainty contributions must äto let the data speak for...

První část této práce se zabývala snižováním emisí toxických látek při spalování tekutých paliv v odpařovacích kotlích. Malé hořákové kotle na tekutá paliva jsou nabízeny na trhu do výkonu 195 kW v nejrůznějším provedení. Některé typy jsou uzpůsobeny ke spalování předehřátých paliv - olejů.Počáteční měření emitovaných toxických látek při spalování...

In August 2002 the centennial water flooded south, west and central part of the Czech Republic. In the most affected areas nearby Elbe River are situated hot-spots of POPs contamination in the Czech Republic: chemical plants SPOLANA and SPOLCHEMIE which produced chlorinated pesticides. In addition, buildings heavily contaminated due to accident com...

V naší předchozí práci jsme na odpařovacím kotli Kroll W401VL o výkonu 11 až 29 kW studovali emise při spalování odpadního oleje z automobilových motorů a převodovek a odpadního hydraulického oleje. Naměřené emisní hodnoty pro polychlorované dibenzo-p-dioxiny a dibenzofurany (PCDD, PCDF) při 11% obsahu kyslíku odpovídaly hodnotám 0.116 a 0.039 TEQ...

In general, two approaches to analyze such complicated groups of compounds as PCDD/F and PCB are widespread - GC-MS/MS or GC/LRMS. Tandem mass spectrometry can be considered to be a special part of LRMS - more selective and sensitive than quadruple MS. Ion trap mass spectrometers are well known for their sensitivity. Several papers, proving that io...

Direct discharge from industrial and municipal wastewater treatment plants into streams has become a growing environmental problem. Most of these wastewaters are complex mixtures containing a lot of inorganic and organic compounds. Their complexity precludes the identification of their potential environmental impact through chemical analyses alone....

Semipermeable membrane devices (SPMDs) have become a powerful tool for ecotoxicological monitoring of persistent organic compounds in the environment. Much research work dealt with detailed description of mechanisms describing the transport of individual compounds from ambient, water and/or air, into the SPMD, which consequently enable to calculate...

The effect of synthetic and mineral oils on the formation of polyaromatic hydrocarbons (PAHs), polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and biphenyls (PCBs) in emissions from a spark ignition engine was studied on a Škoda Favorit engine fueled with leaded gasoline. The test cycle simulated urban traffic conditions on a chas...

Semi-permeable membrane devices (SPMD) are passive sampling devices suitable for concentration of trace contaminants from the environment. In this presentation we summarize results of pilot experiments with applications of the in vitro reporter gene bioassay (H4IIE.luc cells) for assessment of AhR-mediated ("dioxin-like") toxicities of SPMDs expose...

Central Institute for Supervising and Testing in Agriculture, Hroznová 2, 656 06 Brno, Czech Republic 5 AXYS -Varilab spol.s r.o.

One solution of coming crude-oil crisis in the world is using of bio-fuels and other renewable fuels. The share of bio-fuels in the Czech Republic now is very low. Increase of this share is necessary not only for reason mentioned above but too for implementation of Directive 2003/30/EC concerning using of bio-fuels and other renewable fuels in tran...