Tomas Csank

Tomas Csank
University of Veterinary Medicine and Pharmacy in Košice · Department of Microbiology and Immunology

DVM., PhD.

About

55
Publications
19,771
Reads
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216
Citations
Additional affiliations
September 2011 - present
University of Veterinary Medicine and Pharmacy in Košice
Position
  • Researcher/Lecturer
January 2011 - present
University of Veterinary Medicine and Pharmacy in Košice
Education
September 2007 - September 2011
September 2000 - May 2007
University of Veterinary Medicine and Pharmacy in Košice
Field of study
  • General Veterinary Medicine

Publications

Publications (55)
Article
Nowadays, the aquaculture industry is one of the fastest growing industries. Intensive aquaculture has a negative impact on fish health. Probiotic bacteria are often used due to beneficial effect to health of host, e.i. decrease of diseases outbreaks, immunomodulatory effect or better utilization of feed. The aim of this work was to study the influ...
Article
Full-text available
Background Despite the known circulation of West Nile virus (WNV) and Usutu virus (USUV) in Slovakia, no formal entomological surveillance programme has been established there thus far. Aim To conduct contemporaneous surveillance of WNV and USUV in different areas of Slovakia and to assess the geographical spread of these viruses through mosquito...
Article
Full-text available
This study reports on a fatal case of a captive great grey owl infected with the West Nile virus (WNV) in the zoological garden Košice, eastern Slovakia (Central Europe). The tissue samples of the dead owl were used for virus isolation and genetic characterization. The novel isolate is genetically closer to Hungarian, Greek, and Bulgarian strains f...
Article
West Nile virus (WNV) is a mosquito-borne flavivirus (the genus Flavivirus) representing a medical and veterinary public health concern. Birds are the most important reservoirs. Culicine mosquitoes transmit WNV to vertebrate hosts (including horses and humans) and migratory birds play role in its long-distance transport. Slovakia is geographically...
Article
The aim of this paper is to summarize current knowledge concerning the issue of coronaviruses (SARS-CoV and SARS-CoV-2) in terms of their possible source, occurrence and infectivity in the environment as well as its possible inactivation. This review specifically focuses on the issue of selected viruses and wastewater. It should be noted that curre...
Article
Predkladaný príspevok sa snaží sumarizovať najnovšie vedecké poznatky týkajúce sa problematiky koronavírusov (najmä SARS-CoV a SARS-CoV-2) a ich schopnosti kontaminovať pitné a odpadové vody. Článok taktiež špecificky cieli svoju pozornosť na problematiku vybraných vírusov, ktoré sa často vyskytujú v odpadových vodách a popisuje aj možnosti ich úči...
Article
The aim of this work was the genetic typing of RVA isolates originating from pigs and human patients in Slovakia. Seventy-eight rectal swabs from domestic pigs and 30 stool samples from humans were collected. The whole VP7 (G genotypes), VP6 (I genotypes) and partial VP4 (P genotypes) ORFs were amplified by RT-PCR. Genetic variability was higher am...
Article
Searching for specific antibodies in bird serum could reveal arbovirus activity in certain localities. To date, no systematic surveillance for arbovirus infection in autochthonous population of wild birds has been conducted in Slovakia. Present study includes birds captured on spring migration and birds of the autochthonous nesting population of th...
Article
Full-text available
Objective: The aim of this seroepidemiological study was to determine the current prevalence of antibodies against tick-borne encephalitis virus (TBEV) in the representative group of Slovak population with included potential risk factors for TBEV. Methods: Representative group consisted of 428 persons (also with possible exposure to risk factors...
Article
Full-text available
Objective: The aim of the presented cross-sectional seroepidemiological study was to determine the current presence of antibodies against B. burgdorferi s.l. in the groups of Slovak population, and to identify potential risk factors to Lyme borreliosis. Methods: A group of 261 adults (patients from the Neurological Clinic with possible symptoms...
Article
Objective: The aim of the study was to evaluate the seroprevalence of West Nile virus (WNV) among the variable population of Eastern Slovakia. Methods: A serologic survey was conducted using 464 serum samples. The basic demographic, epidemiologic and clinical information was obtained for each serum sample at the time of specimen collection. The...
Article
The present study reports the first complete genome sequence analysis of West Nile virus (WNV) strains isolated from brain samples from raptors. The results prove the circulation of closely related WNV lineage II strains in central Europe and genetic analysis revealed seven amino acid substitutions in structural (PrM3, E159 and E231) and in non-str...
Article
In our previous study we confirmed an antiviral activity of probiotic Lactobacillus reuteri L26 which was mediated by stimulation of local intestinal immunity. The aim of this paper was to evaluate the influence of L. reuteri L26 on the systemic immune response in gnotobiotic mice infected with porcine circovirus type 2 (PCV2). A total of 30 germ-f...
Conference Paper
Full-text available
The aim of this work was to study the population dynamics of breeding avifauna on the locality of Drienovská mokraď wetland (GPS coordinates 48°36′27″N 20°57′07″E) using „Constant Effort Site Ringing“ methodology (CES). Part of the work was the screening of a seroprevalence of antibodies against the West Nile Virus (WNV) in local birds.
Article
Full-text available
Lake Geča is located in the northern part of the Protected Bird Territory Košická kotlina. With a surface area of 250 ha it is the largest water area in the complex of a number of gravel lake areas located close to the villages Čaňa and Geča. From 2014—2016 we used the method of capture and ringing to focus on observations of transmigrating passeri...
Article
Full-text available
In central Europe, at least three flaviviruses circulate among vectors and vertebrate hosts. West Nile virus (WNV) and Usutu virus (USUV) are mosquito-borne viruses maintained in the nature by enzootic cycle between mosquitoes and birds. Tick-borne encephalitis virus (TBEV) is a flavivirus causing annual human cases in Slovakia. The aim of this stu...
Article
Full-text available
The tick-borne encephalitis virus (TBEV) and West Nile virus (WNV) are arboviruses of the genus Flavivirus in the family Flaviviridae. Their hosts are vertebratesof which rodents are the reservoirs of TBEV and birds are the reservoirs of WNV. Both viruses are transmitted from reservoirs to mammals by vectors. TBEV is transmitted by ticks (mostly Ix...
Presentation
Full-text available
Anthocyanins represent the major group of flavonoids in berries as well as one of the most powerful natural antioxidants. The aim of this study was to analyse immunomodulatory activities of purified anthocyanins-rich elderberry extract using porcine epithelial IPEC-1 cells and dendritic cells differentiated from porcine blood monocytes (moDC). We e...
Article
Full-text available
Probiotic bacteria are frequently used for prevention of bacterial infections of the gastrointestinal tract, but there are only limited studies on their efficacy against viral gut infections in animals. The aim of this study was to investigate the effect of probiotic Lactobacillus reuteri L26 Biocenol(TM) on the innate and adaptive immune responses...
Presentation
Full-text available
The presentation is about detection and isolation of TRBV from ticks after 50 years. The tick were collected from grazing goats. The new locality is different from the original one. Genetic analysis showed realtionship with the original strain from Slovakia and with two strains from Ukraine. A close relationship is with Lipovník virus. However, dee...
Conference Paper
Full-text available
Tick-borne encephalitis virus (TBEV, Flaviviridae, Flavivirus) and Tribeč virus (TRBV, Reoviriae, Orbivirus) are arboviruses transmitted by ticks. Tribeč virus is a member of the Great Island virus serogroup. Both viruses cause serous meningoencephalitis. Here, we describe the occurrence of TRBV and TBEV in Ixodes ricinus in Dúbrava district (Prešo...
Conference Paper
Full-text available
Tribeč virus (TRBV) together with Lipovník and Kemerovo viruses are members of the Great Island virus serogroup in the genus Orbivirus (Reoviridae). TRBV was first time isolated in Slovakia from nymphs of Ixodes ricinus collected in Tribeč Mountains, from rodents and sentinel goats. Herein, we focus on the prevalence of TRBV neutralizing antibodies...
Poster
Full-text available
Arboviruses (an acronym for “arthropod-borne viruses”) form an ecological but not taxonomic grouping and there are about 500 of them worldwide, belonging to nine families. Tick-borne encephalitis virus (TBEV), West Nile virus (WNV) and Usutu virus (USUV) are viruses belonging to family Flaviviridae (Flavivirus), which have the greatest medical and...
Article
West Nile virus (WNV) is a mosquito-borne neurotropic pathogen that presents a major public health concern. Information on WNV prevalence and circulation in Slovakia is insufficient. Oral and cloacal swabs and bird brain samples were tested for flavivirus RNA by RT-PCR using newly designed generic primers. The species designation was confirmed by s...
Article
Native polyacrylamide gel electrophoresis at pH 8.8 did not allow to separate lactate dehydrogenase isoenzymes of turkey origin. Five electrophoretically distinguishable forms of the enzyme were detected in serum and tissues of turkey using isoelectric focusing technique in a pH range of 3 to 9. Generally, three different groups were seen: a) those...
Article
Full-text available
Classical swine fever is a highly contagious viral disease that affects domestic and wild suids and could cause important economic losses. It is the most dangerous infectious disease of the wild boar that can cause severe death in densely populated areas. The aim of the study was to determine the effect of endoparasites on the oral vaccination agai...
Article
BACKGROUND Anthocyanins, compounds that represent the major group of flavonoids in berries, are one of the most powerful natural antioxidants. The aim of this study was to evaluate biological activities and comparison of anthocyanin-rich extracts prepared from chokeberry (Aronia melanocarpa), elderberry (Sambucus nigra), bilberry (Vaccinium myrtill...
Article
Full-text available
The aim of this study was to investigate the toxic effect of the metal salt cadmium chloride dihydrate on the rabbit kidney cell line using the xCELLigence system or real-time cell analyser (RTCA), and to compare this relatively new method with standard biological cytotoxicity assays. This system provides real-time monitoring of cell behaviour and...
Chapter
Organophosphates are used frequently in Europe for pest control due to their low price and broad spectrum of activity. In 2003, they accounted for over 59% (4645 tonnes) of insecticide sales in the EU, with chlorpyrifos the top selling insecticide (15.6%, 1226 tonnes). Chlorpyrifos was also one of the most widely used organophosphates in the US for...
Article
Abbreviations: ISH = in situ hybridisation; PCV-2 = porcine circovirus 2; PMWS = postweaning multisystemic wasting syndrome Porcine circovirus 2 (PCV-2) is a causative agent of several diseases of pigs that are collectively termed porcine circovirus diseases. The virus has a single-stranded DNA genome of a size approximately 1.8 kb, virions of 17–2...
Conference Paper
Full-text available
Abstrakt Expresia domény III obalového proteínu vírusu západonílskej horúčky (West Nile virus) pomocou pQE-30 UA-mCherry-GFP vektora a jej využitie v detekcii WNV protilátok veterinárskeho lekárstva a farmácie v Košiciach, Košice 2 Neuroimunologický ústav SAV, Bratislava csank@uvlf.sk Vírus západonílskej horúčky (West Nile virus – WNV) je neurotróp...
Article
Full-text available
In the present study three different cell cultures, derived from rabbit kidney (RK13), rat liver (WBF344) and insect origin (Sf21), were used to examine the cytotoxic effect of the insecticide endosulfan. Cytotoxicity was determined on the basis of cell proliferation activity; the cellular damage was assessed by evaluation of cytopathic effect and...
Article
Porcine circovirus 2 (PCV-2) is a primary agent of post-weaning multi-systemic wasting syndrome (PMWS), ubiquitous in pig herds. The course of viraemia and seroconversion in naturally infected pigs were investigated in piglets from the 2nd week of their life. Piglets were divided into seropositive (Ab(+) ) and seronegative (Ab(-) ) groups. Subseque...
Conference Paper
Full-text available
Invasion of different host niches by Borrelia is associated with its translocation across various the body barriers. Neuroinvasive character is in part attributed with crossing of the blood–brain barrier (BBB). This translocation is a multi-stage process with unclear molecular principle. Main prerequisite of successful borrelial traversal seems to...
Article
Full-text available
There is an increasing need for rapid and easily interpreted in vitro assays to screen for possible cytotoxicity of pesticides. The objective of this study was to investigate the effect of the carbamate insecticide bendiocarb on mammalian and insect cell cultures. The cytotoxicity of this insecticide was evaluated by cell proliferation and cellular...
Article
Full-text available
Neuroborreliosis can arise at any time during the course of Lyme disease. Neurological manifestations are attributed in part to penetration of the blood-brain barrier (BBB) and invasion of the central nervous system. The main prerequisite for successful BBB translocation is stationary adhesion to surface of endothelial cells mediated by ligand:rece...
Article
Full-text available
In the present study four different cell cultures, derived from rabbit kidney (RK13), rat; and murine liver (WBF344 and Hepa 1c1c7) and insect origin (Sf21) were used to examine the effects of chlorpyrifos. Sf21 cells were the most sensitive to chlorpyrifos, with significant suppression of their proliferative activity ranging from 10(-1)-10(-5) M....
Conference Paper
Leishmania tarentolae is a trypanosomatid protozoan host isolated from Moorish gecko Tarentola mauritanica [1], non-pathogenic to mammals, and can be used as a protein expression system. Proteins expressed by Leishmania tarentolae possess post-translation modifications close to mammalian-type, such as glycosylation, phosphorylation or prenylation....
Article
The prevalence of porcine circovirus 2 (PCV-2) infection in the pig population in Slovakia was investigated. Sera from pigs suspected for post-weaning multisystemic wasting syndrome (PMWS) as well as clinically healthy pigs were tested for viral DNA and specific IgM and IgG antibodies. Pigs (n = 198) were categorized to weaning, grower and fattenin...
Article
Letter to the Editor No abstract Keywords: Bovine viral diarrhea virus 1 and 2; cell line susceptibility; virus recovery.

Questions

Questions (3)
Question
Dear Camille.
The aim of your project is similar to our goals.
Our PhD. student work with Tribeč virus. Currently, we have isolated a strain of TRBV. We were curious how does it replicate in different host cell lines. During 25 serial passages in three different cell lines (VeroE6, A549 and BHK21), we have observed the presence of 3 sizes of plaques. Now, we would like to isolate them and genetically characterize. I do not have experience with plaque isolation. If you do, please, could you describe how to do it?
We use 2% carboxymethylcellulose for plaque formation test.
We would also like to do the same in tick cell line, but I did not find any in cell culture collection.
With best regards,
Tomas.
Question
After 24 hrs of VERO cell line thaw, we observed granules in the cell cytoplasm (attachment VERO_24hrs). The cells are in Eagle´s MEM with 10% FBS, 2 mM L-Glutamine and 50 ug/ml Gentamycin. At the same time with the same medium we thawed PK15 cells and these look fine, without any changes in cytoplasm (PK15_24hrs). I have few experiences with VERO cell line, is this normal for these cells? I don´t think it is mycoplasma, because the granules are in the cytoplasm. The black spots, which looks like in the medium are dirt on the optics :) The viability of the cells at the time of freezing (2 years ago) was 96% and the liquid nitrogen was confluently supplemented during the storage.
Does anybody expirienced this, or do you have any suggestion what may cause this?
Thank you in advance.
Question
I have a problem with cultivation of bovine turbinate cells on 48 well plate. Approx. 1 hour after medium change I have cytotoxicity in some wells on the side of the plate. Do you have any idea what´s happened to my cells?

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Projects

Project (1)
Project
The project is focused on the circulation of WNV in free living and game birds, horses, humans and in vectors. Serum and tissue/mosquitoe samples will be examined by serological and molecular methods, respectivelly.