Tomáš Bertók

Tomáš Bertók
Slovak Academy of Sciences | SAV · Department of Glycobiotechnology

PhD., EUR ING

About

77
Publications
19,737
Reads
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1,689
Citations
Introduction
Microbiology and molecular biology, bioprocess engineering, immobilization processes, industrial biotechnology; Bioanalytical chemistry, biosensors and biochips, medical engineering, electrochemistry, nanobiotechnology, proteomics and glycomics
Additional affiliations
September 2016 - September 2016
Institute of Biophysics ASCR
Position
  • Visiting research scientist
Description
  • PSA detection and glycoprofilation on HMDE
February 2016 - February 2016
Institute of Biophysics ASCR
Position
  • Visiting research scientist
Description
  • CHER on HMDE for glycomic analyses
September 2015 - September 2015
Institute of Biophysics ASCR
Position
  • Visiting research scientist
Description
  • Osmium complexes for glycomic analyses
Education
October 2014 - October 2014
European Federation of National Engineering Associations
Field of study
  • Chemical Technology
September 2010 - August 2014
September 2005 - June 2010

Publications

Publications (77)
Article
Exosomes are considered to be a rich source of biomarkers, hence in this article we examine the best procedure for their isolation. We examine several isolation procedures, exosome storage conditions and other conditions affecting exosome production by prostate cell lines. We selected four different commercially available kits based on different pr...
Article
Full-text available
Background: Testicular cancer (TC) is the most frequent type of cancer among young men aged between 15 and 34 years. TC is treated using cisplatin, but 3%-5% of TC patients fail to respond to cisplatin, with a very bad to fatal prognosis. Accordingly, it is most important to quickly and readily identify those TC patients who are resistant to cispl...
Article
Full-text available
Colorectal cancer (CRC) is one of the most common types of cancer among men and women worldwide. Efforts are currently underway to find novel and more cancer-specific biomarkers that could be detected in a non-invasive way. The analysis of aberrant glycosylation of serum glycoproteins is a way to discover novel diagnostic and prognostic CRC biomark...
Article
Full-text available
The review initially provides a short introduction to prostate cancer (PCa) incidence, mortality, and diagnostics. Next, the need for novel biomarkers for PCa diagnostics is briefly discussed. The core of the review provides details about PCa aetiology, alternative biomarkers available for PCa diagnostics besides prostate specific antigen and their...
Article
In the field of printed electronics, carbon and its allotropes are today among the most studied materials due to their unique physical and chemical properties. In this work, carbon dispersions were used for the preparation of screen-printed carbon electrodes applied as counter electrodes (CEs) for the dye-sensitized solar cells and as working elect...
Article
Full-text available
Prostate cancer (PCa) is one of the most common cancer types among men and also a common cause of death globally. With an increasing incidence, there is a need for low-cost, reliable biomarkers present in samples, which could be provided non-invasively (without a need to perform prostate biopsy). Glycosylation changes of free-PSA (fPSA) are conside...
Article
The review provides articles discussing big challenges behind successful design of impedimetric biosensors and the way such challenges were/could be addressed for highly sensitive affinity detection of proteins. In particular, the effect of gold etching by chloride ions or by CN⁻ ions (released from the redox probe [Fe(CN)6]3-/4-), the effect of io...
Article
Full-text available
This is the first work focused on glycoprofiling of whole N- and O- glycome using lectins in an array format applied for analysis of serum samples from healthy individuals, benign prostate hyperplasia (BPH) patients, and prostate cancer (PCa) patients. Lectin microarray was prepared using traditional lectins with the incorporation of 2 recombinant...
Article
Full-text available
Background: To compare the clinical performance of a new PCa serum biomarker based on fPSA glycoprofiling to fPSA% and PHI. Methods: Serum samples from men who underwent prostate biopsy due to increased PSA were used. A comparison between two equal groups (with histologically confirmed PCa or benign, non-cancer condition) was used for the clinical...
Article
Full-text available
For efficient and effective utilization of MXene such as biosensing or advanced applications, interfacial modification of MXene needs to be considered. To this end, we describe modification of Ti3C2Tx MXene by aryldiazonium-based grafting with derivatives bearing a sulfo- (SB) or carboxy- (CB) betaine pendant moiety. Since MXene contains free elect...
Article
Full-text available
Two-dimensional layered nanomaterial Ti3C2TX (a member of the MXene family) was used to immobilise enzyme sarcosine oxidase to fabricate a nanostructured biosensor. The device was applied for detection of sarcosine, a potential prostate cancer biomarker, in urine for the first time. The morphology and structures of MXene have been characterised by...
Article
Invited for this month's cover picture is the group of Dr. Jan Tkac (head of the Department of Glycobiotechnology at the Institute of Chemistry at the Slovak Academy of Sciences, Slovakia). The cover picture shows a common eukaryotic cell with basic organelles, as well as intracellular formation/preparation of exosomes for express delivery to dista...
Article
The Front Cover illustrates a common eukaryotic cell with basic organelles, as well as intracellular formation/preparation of exosomes for express delivery to distant tissues.More information can be found in the Review by L. Lorencova et al.
Article
Exosomes are naturally produced biological nanoparticles secreted by cells into body fluids and responsible for intercellular communication. Exosomes can also carry cargo affecting neighbouring cells and forming pre‐metastatic niches. Hence, exosomes are behind localised tumour development, progression, but also the induction of distant tumours for...
Article
2D MXenes as Perspective Immobilization Platforms for Design of Electrochemical Nanobiosensors. More details can be found in the Full Paper by Ludmila Šimková, Jan Svoboda, Jiři Pinkas, et al. (DOI: 10.1002/elan.201900288).
Article
Full-text available
The article describes preparation, characterization and further modification of hybrid magnetic particles (Au nanoshells with a magnetic core (MPs@silica@Au)) by zwitterionic molecules bearing diazonium functional groups. Such hybrid magnetic particles modified by zwitterionic molecules exhibit the following features: •Responsiveness towards extern...
Article
MXenes are a new group of 2D nanomaterials with fascinating properties including high electrical conductivity, hydrophilic nature, easily tunable structure and high surface area. This is why MXene modified interfaces are extremely promising for the preparation of sensitive electrochemical biosensors. While there are numerous reports on MXene‐based...
Article
The initial part of this review details the controversy behind the use of a serological level of prostate-specific antigen (PSA) for the diagnostics of prostate cancer (PCa). Novel biomarkers are in demand for PCa diagnostics, outperforming traditional PSA tests. The review provides a detailed and comprehensive summary that PSA glycoprofiling can e...
Article
In this paper several advances were implemented for glycoprofiling of prostate specific antigen (PSA), what can be applied for better prostate cancer (PCa) diagnostics in the future: 1) application of Au nanoshells with a magnetic core ([email protected]@Au); 2) use of surface plasmons of Au nanoshells with a magnetic core for spontaneous immobiliz...
Article
The Cover Feature shows integration of electrochemical impedance spectroscopy for the analysis of clinically relevant samples toward cancer diagnostics.More information can be found in the Review by T. Bertok et al.
Article
Introduction: Prostate cancer (PCa) is a life-threatening disease affecting millions of men. The current best PCa biomarker (level of prostate-specific antigen in serum) lacks specificity for PCa diagnostics and this is why novel PCa biomarkers in addition to the conventional ones based on biomolecules such as DNA, RNA and proteins need to be iden...
Article
Impedimetric affinity biosensors are, without any doubt, among the most sensitive analytical devices available, offering low limits of detection and wide linear response ranges. There are, however, few papers detailing the application of impedimetric biosensors for the analysis of clinically relevant samples with due clinical performance. The fact...
Article
There are two key novel components presented here behind identification of potential novel breast cancer (BCa) diagnostic approach: 1. application of photoimmobilizable zwitterionic hydrogels resisting non-specific protein adsorption for preparation of the biosensor interfaces and 2. integration of lectins (carbohydrate recognizing proteins) within...
Article
Electrochemical performance of a 2D Ti3C2Tx (MXene, where T: [dbnd]O, –OH, –F) sheets modified with Pt nanoparticles (PtNPs) was investigated. The results showed that Ti3C2Tx/PtNP nanocomposite deposited on the surface of GCE showed much better and stable redox behavior in an anodic potential window as compared to the GCE modified with pristine Ti3...
Article
In this study, one hundred serum samples from healthy people and patients with rheumatoid arthritis (RA) were analyzed. Standard immunoassays for detection of 10 different RA markers and analysis of glycan markers on antibodies in 10 different assay formats with several lectins were applied for each serum sample. A dataset containing 2000 data poin...
Article
Films made of xylan (X) and quaternized chitosan (QC) were prepared and the interactions of the polysaccharides on interfaces were discussed. According to elemental analysis, the X film contained also cellulose (31%) which could not be separated from the water suspension. The QC sample was soluble in water due to the presence of quaternized glucosa...
Article
In this study, two quaternary ammonium salts derived from l-lipoic acid were applied for self-assembled monolayers formation on rough structured gold surface. The derivatives differ in functionality since one possesses simple quaternary ammonium group whereas the other one is carboxybetaine ester containing quaternary ammonium group with pH hydroly...
Article
Full-text available
Screening serum for the presence of prostate specific antigen (PSA) belongs to the most common approach for the detection of prostate cancer. This review (with 156 refs.) addresses recent developments in PSA detection based on the use of various kinds of nanomaterials. It starts with an introduction into the field, the significance of testing for P...
Article
We describe a self-assembled monolayer (SAM) on a gold surface with carboxybetaine ester functionality to control the interaction between DNA and gold nanoparticles via pH. The negatively charged phosphate backbone of DNA interacts with and adsorbs to the positively charged carboxybetaine esters on the SAM. DNA release can be achieved by the hydrol...
Article
In recent decades, it has become clear that most of human proteins are glycosylated and that protein glycosylation plays an important role in health and diseases. At present, simple, fast and inexpensive methods are sought for clinical applications and particularly for improved diagnostics of various diseases, including cancer. We propose a label-...
Article
Full-text available
This review comprehensively covers the most recent achievements (from 2013) in the successful integration of nanomaterials in the field of glycomics. The first part of the paper addresses the beneficial properties of nanomaterials for the construction of biosensors, bioanalytical devices, and protocols for the detection of various analytes, includi...
Article
Full-text available
An extensive characterization of pristine and oxidized Ti3C2Tx (T: O, OH, F) MXene showed that exposure of MXene to an anodic potential in the aqueous solution oxidizes the nanomaterial forming TiO2 layer or TiO2 domains with subsequent TiO2 dissolution by F⁻ ions, making the resulting nanomaterial less electrochemically active compared to the pris...
Article
In our study we used holocelluloses from sugar beet and bagasse for film preparation. Films from sugar beet holocellulose have better mechanical properties than from bagasse holocellulose. By subsequent carboxymethylation of bagasse holocellulose, films with better properties were produced. Specimens prepared from combined sugar beet and bagasse ca...
Article
Full-text available
DOI: 10.1002/pmic.201500463 Mass spectrometry analysis of different linkages sialic acid is attached to the glycan backbone is a daunting task requiring derivatisation steps. In this work we applied lectins specifically recognizing either α-(2,3)- or α-(2,6)-linked terminal sialic acid for specific glycoprofiling of a prostate specific antigen (a p...
Article
Full-text available
Impedimetric lectin biosensor for detection of changes in the glycan structure of antibodies isolated from human serum is here correlated with progression of rheumatoid arthritis (RA). The biosensor was built-up from a mixed self-assembled monolayer (SAM) on gold consisting of two different thiolated zwitterionic derivatives – carboxybetaine and su...
Article
Full-text available
A simple fabrication method for preparation of surfaces able to switch from superhydrophobic to superhydrophilic state in a reversible and fast way is described. A self-assembled monolayer (SAM) consisting of quaternary ammonium group with aliphatic tail bearing terminal thiol functionality was created on gold nano/micro structured and gold planar...
Chapter
The most attention, from all carbonaceous nanomaterials, is currently being paid to graphene-2D, one-atom-thick, highly ordered carbon sheets with excellent electrochemical, thermal, optical, and mechanical properties. From an application point of view, a good biocompatibility of graphene and its derivatives is highly appreciated and increasingly e...
Article
Full-text available
The construction of a sensitive electrochemical lectin-based immunosensor for detection of a prostate specific antigen (PSA) is shown here. Three lectins with different carbohydrate specificities were used in this study to glycoprofile PSA, which is the most common biomarker for prostate cancer (PCa) diagnosis. The biosensor showed presence of α-L-...
Conference Paper
A lot of strategies for smart approaches on surfaces were applied such as hydrogel layer, polymer brushes or self-assembly monolayers (SAM). [1] Nowadays switchable zwitterionic materials consisting of molecules with internally balanced charge between positive ammonium and negative carboxy group are promising candidates for this application. [2] Th...
Chapter
Immobilization of glycans (complex carbohydrates) on surfaces is a tool on how to mimic exposure of glycans on proteins and cells to study their interaction with proteins, viral particlesg or human cells revealing details about pathological processes. Glycan biochips were successfully applied in such studies, but this technology requires presence o...
Article
Full-text available
An ultrasensitive impedimetric glycan-based biosensor for reliable and selective detection of inactivated, but intact influenza viruses H3N2 was developed. Such glycan-based approach has a distinct advantage over antibody-based detection of influenza viruses since glycans are natural viral receptors with a possibility to selectively distinguish bet...
Article
Full-text available
This study presents a proof-of-concept for the development of an impedimetric biosensor for ultra-sensitive glycoprofiling of prostate specific antigen (PSA). The biosensor exhibits three unique characteristics: (1) analysis of PSA with limit of detection (LOD) down to 4 aM; (2) analysis of the glycan part of PSA with LOD down to 4 aM level and; (3...
Article
Full-text available
Impedimetric lectin biosensors capable of recognising two different carbohydrates (galactose and sialic acid) in glycans attached to antibodies isolated from human serum were prepared. The first step entailed the modification of a gold surface by a self-assembled monolayer (SAM) deposited from a solution containing a carboxybetaine-terminated thiol...
Article
Full-text available
Impedimetric glycan biosensor with optimised glycan density was applied for detection of lectins and influenza hemagglutinins down to attomolar concentrations (aM).
Article
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Significant progress has been done in the electrochemical (EC) analysis of practically all proteins, based on the electroactivity of amino acid (aa) residues in proteins. From a transducer point of view, it can be anticipated that many different ways of how nanomaterials can be integrated into the EC detection platform of detection will be develope...
Article
Full-text available
Gluconobacter oxydans bacteria exhibit a unique metabolism for quick and incomplete oxidation of a wide range of different compounds (aldoses, ketoses, mono- and poly-alcohols, etc.). Such biotransformation efficiency with simple biomass production led to the industrial applications of these bacteria in the production of several important commoditi...
Article
Full-text available
Glycans and other saccharides attached to proteins and lipids, are involved in numerous physiological or pathological processes. The changes in glycosylation are usually associated with some pathological states (such as cancer), therefore these alterations are useful tools in diagnostics. This review gives basic insight into glycomics, describes th...
Article
Full-text available
Saccharides play important roles in organisms. Changes in glycosylation and in structure of glycans can induce many diseases or can be induced by diseases. Glycan biochips and biosensors are novel techniques the main role of which is to study the glycan – protein interactions. In the construction of biochips and biosensors it is important to create...
Article
Full-text available
In this mini-review the most advanced patterning protocols and transducing schemes for the development of ultrasensitive label-free and label-based lectin biosensors for the glycoprofiling of disease markers and some cancerous cells are described. The performance of such lectin biosensors with interfacial properties tuned to the nanoscale are criti...