Tobias Goris

Tobias Goris
German Institute of Human Nutrition | DIfE · Group of Intestinal Microbiology

Dr.

About

46
Publications
24,566
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
852
Citations
Citations since 2016
37 Research Items
613 Citations
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
Introduction
My research is focussed on microbial physiology. Currently, I am working on flavonoid modification by gut bacteria and the gut microbiome in the framework of the EU project SynBio4Flav (https://synbio4flav.eu/). Side projects are organohalide respiration and hydrogenases. Methods I use are comparative/functional genomics, physiological (e.g. differential growth) studies, heterologous expression of enzymes and biochemical assays/chromatography.
Additional affiliations
April 2019 - present
German Institute of Human Nutrition
Position
  • PostDoc Position
June 2011 - January 2018
Friedrich Schiller University Jena
Position
  • PostDoc Position
Description
  • Genomics of organohalide-respiring bacteria, DFG research unit 1530
December 2005 - May 2011
Humboldt-Universität zu Berlin
Position
  • Doctoral Thesis
Description
  • Influence of a novel Fe-S cluster on oxygen stability of the membrane-bound hydrogenase from Ralstonia eutropha
Education
April 2003 - August 2005
Humboldt-Universität zu Berlin
Field of study
  • Biology
October 2000 - March 2003
University of Bonn
Field of study
  • Biology

Publications

Publications (46)
Article
Full-text available
Background The rise of antibiotic resistance (AR) in clinical settings is of great concern. Therefore, the understanding of AR mechanisms, evolution, and global distribution is a priority for patient survival. Despite all efforts in the elucidation of AR mechanisms in clinical strains, little is known about its prevalence and evolution in environme...
Article
Full-text available
Coronavirus-related disease 2019 (COVID-19) became a pandemic in February 2020, and worldwide researchers try to tackle the disease with approved drugs of all kinds, or to develop novel compounds inhibiting viral spreading. Flavonoids, already investigated as antivirals in general, also might bear activities specific for the viral agent causing COV...
Article
Full-text available
Flavonoids are a major group of dietary plant polyphenols and have a positive health impact, but their modification and degradation in the human gut is still widely unknown. Due to the rise of metagenome data of the human gut microbiome and the assembly of hundreds of thousands of bacterial metagenome-assembled genomes (MAGs), large-scale screening...
Article
Full-text available
Microbial communities involving dehalogenating bacteria assist in bioremediation of areas contaminated with halocarbons. To understand molecular interactions between dehalogenating bacteria, we co-cultured Sulfurospirillum multivorans, dechlorinating tetrachloroethene (PCE) to cis−1,2-dichloroethene (cDCE), and Dehalococcoides mccartyi strains BTF0...
Article
Full-text available
Energy conservation via organohalide respiration (OHR) in dehalogenating Sulfurospirillum species is an inducible process. However, the gene products involved in tetrachloroethene (PCE) sensing and signal transduction have not been unambiguously identified. Here, genome sequencing of Sulfurospirillum strains defective in PCE respiration and compara...
Article
Full-text available
Self‐transferable plasmids of the incompatibility group P‐1 (IncP‐1) are considered important carriers of genes for antibiotic resistance and other adaptive functions. In the laboratory, these plasmids have a broad host range; however, little is known about their in situ host profile. In this study, we discovered that Thauera aromatica K172T, a fac...
Article
Full-text available
Background The high incidence of bacterial genes that confer resistance to last-resort antibiotics, such as colistin, caused by mobilized colistin resistance (mcr) genes, poses an unprecedented threat to human health. Understanding the spread, evolution, and distribution of such genes among human populations will help in the development of strategi...
Poster
Full-text available
Flavonoids are a major group of dietary plant polyphenols and have a positive health impact, but their modification in the human gut is still widely unknown (Figure 1). Here, we screened the Human Gastrointestinal Genome collection including more than 280,000 bacterial metagenome-assembled genomes (MAGs) for potential flavonoid-modifying enzym...
Article
Full-text available
Bacteria often employ different respiratory chains that comprise membrane proteins equipped with various cofactors. Monitoring the protein inventory that is present in the cells under a given cultivation condition is often difficult and time‐consuming. One example of a metabolically versatile bacterium is the microaerophilic organohalide‐respiring...
Article
Full-text available
The genus Desulfoluna comprises two anaerobic sulfate-reducing strains, D. spongiiphila AA1T and D. butyratoxydans MSL71T, of which only the former was shown to perform organohalide respiration (OHR). Here we isolated a third strain, designated D. spongiiphila strain DBB, from marine intertidal sediment using 1,4-dibromobenzene and sulfate as the e...
Article
Full-text available
Hydrogen-producing bacteria are of environmental importance, since hydrogen is a major electron donor for prokaryotes in anoxic ecosystems. Epsilonproteobacteria are currently considered to be hydrogen-oxidizing bacteria exclusively. Here, we report hydrogen production upon pyruvate fermentation for free-living Epsilonproteobacteria, Sulfurospirill...
Article
Full-text available
Despite the wealth of physiological knowledge and plentiful genomes available, only few natural products of anaerobic bacteria have been identified until today and even less have been linked to their biosynthetic gene cluster. Here, we analyzed a unique NRPS-PKS hybrid gene cluster from an anaerobic Epsilonproteobacterium (Sulfurospirillum barnesii...
Article
Full-text available
Significance: The assessment of enzyme-substrate or protein-ligand interactions in organohalide-respiring bacteria is a fundamental challenge. Thermal proteome profiling (TPP) allows elucidating proteome-wide thermal stability changes relying on the sensitivity of modern mass spectrometry. This gives access to the identification of interactions no...
Article
Full-text available
Significance: The so far unique long-term downregulation of organohalide respiration is now identified in a second species suggesting a broader distribution of this regulatory phenomenon. An improved protein extraction method allowed the identification of proteins most probably involved in transcriptional regulation of OHR in Sulfurospirillum spp....
Article
Full-text available
Two anaerobic bacterial consortia, each harboring a distinct Sulfurospirillum population, were derived from a ten year old consortium, SL2, previously characterized for the stepwise dechlorination of tetrachloroethene (PCE) to cis-dichloroethene (cis-DCE) via accumulation of trichloroethene (TCE). Population SL2-1 dechlorinated PCE to TCE exclusive...
Article
Full-text available
Organohalide respiration (OHR) is a crucial process in the global halogen cycle and of interest for bioremediation. However, investigations on OHR are hampered by the restricted genetic accessibility and the poor growth yields of many organohalide-respiring bacteria (OHRB). Therefore, genomics, transcriptomics and proteomics are often used to inves...
Preprint
Full-text available
Hydrogen-producing bacteria are of environmental and biotechnological importance in anoxic environments, since hydrogen is an important electron donor for prokaryotes and of interest as an alternative energy source. Epsilonproteobacteria, inhabiting ecologically, clinically or biotechnologically relevant environments, are currently considered to be...
Article
Full-text available
Reductive dehalogenation of organohalides is carried out by organohalide-respiring bacteria (OHRB) in anoxic environments. The tetrachloroethene (PCE)-respiring Epsilonproteobacterium Sulfurospirillum multivorans is one of few OHRB able to respire oxygen. Therefore, we investigated the organism's capacity to dehalogenate PCE in the presence of oxyg...
Article
Full-text available
The Desulfitobacterium genus comprises anaerobic Gram-positive bacteria, of which the majority are facultative organohalide-respirers. We here present the genomes of eight strains of Desulfitobacterium spp., including five strains of D. hafniense, one strain each from D. dichloroeliminans and D. metallireducens, and one strain that had not been ass...
Article
Sulfurospirillum halorespirans is a bacterium that couples the reductive dehalogenation of chlorinated ethenes to growth. This process is called organohalide respiration (OHR), which can be of importance for bioremediation. Here, we report the complete genome of S. halorespirans, the second one of an organohalide-respiring Epsilonproteobacterium af...
Article
Full-text available
The organohalide-respiring Epsilonproteobacterium Sulfurospirillum multivorans is able to grow with hydrogen as electron donor and with tetrachloroethene (PCE) as electron acceptor; PCE is reductively dechlorinated to cis-1,2-dichloroethene. Recently, a genomic survey revealed the presence of four gene clusters encoding NiFe hydrogenases in its gen...
Article
Full-text available
Tetrachloroethene reductive dehalogenase (PceA), a corrinoid-containing enzyme from Sulfurospirillum multivorans, is highly active for the sequential reduction of the organohalide tetrachloroethene (PCE) to trichloroethene (TCE), then regiospecifically to cis-1,2-dichloroethene (cDCE). We demonstrate direct electron transfer from graphite and semic...
Chapter
Full-text available
The only organohalide-respiring Epsilonproteobacteria (ε-proteobacteria) described so far are found in the genus Sulfurospirillum . This genus consists of versatile, often microaerophilic bacteria, growing with many different growth substrates. Only a few of these organisms use halogenated compounds, mainly chlorinated ethenes, as electron acceptor...
Article
Full-text available
Sulfurospirillum multivorans is a free-living, physiologically versatile Epsilonproteobacterium able to couple the reductive dehalogenation of chlorinated and brominated ethenes to growth (organohalide respiration). We present proteomic data of S. multivorans grown with different electron donors (formate or pyruvate) and electron acceptors (fumarat...
Article
Full-text available
Organohalide respiration is an environmentally important but poorly characterized type of anaerobic respiration. We compared the global proteome of the versatile organohalide-respiring Epsilonproteobacterium Sulfurospirillum multivorans grown with different electron acceptors (fumarate, nitrate, or tetrachloroethene [PCE]). The most significant dif...
Data
Full-text available
NOTE: This is a corrected version of the supplement to "Proteomics of the organohalide-respiring Epsilonproteobacterium Sulfurospirillum multivorans adapted to tetrachloroethene and other energy substrates" which is found at http://www.nature.com/articles/srep13794 Changes: Values in table S5 should read with decimal marks (i.e. 1.328 instead of 1,...
Article
Full-text available
This genome report describes the draft genome and the physiological characteristics of Desulfitobacterium hafniense PCE-S, a Gram-positive bacterium known to dechlorinate tetrachloroethene (PCE) to dichloroethene by a PCE reductive dehalogenase. The draft genome has a size of 5,666,696 bp with a G + C content of 47.3%. The genome is very similar to...
Article
Full-text available
Sulfurospirillum multivorans, a free-living ε-Proteobacterium, is among the best studied organisms capable of organohalide respiration. It is able to use several halogenated ethenes as terminal electron acceptor. In this report, the complete genome sequence of S. multivorans including a comparison with genome sequences of two related non-dehalogena...
Article
Full-text available
The genomes of two novel Dehalococcoides mccartyi strains, DCMB5 and BTF08, enriched from the heavily organohalide-contaminated megasite around Bitterfeld (Germany), were fully sequenced and annotated. Although overall similar, the genome sequences of the two strains reveal remarkable differences in their genetic content, reflecting a specific adap...
Data
Full-text available
Hydrogenasen sind essentielle Enzyme im mikrobiellen H2-Kreislauf und werden als vielversprechende Katalysatoren in biologisch basierten H2-Technologien angesehen. Ein entscheidender Nachteil vieler Hydrogenasen ist ihre hohe O2-Sensitivität. Die membrangebundene Hydrogenase (MBH) aus Ralstonia eutropha ist eines der wenigen Beispiele für Hydrogena...
Article
Full-text available
Hydrogenases are essential for H(2) cycling in microbial metabolism and serve as valuable blueprints for H(2)-based biotechnological applications. However, most hydrogenases are extremely oxygen sensitive and prone to inactivation by even traces of O(2). The O(2)-tolerant membrane-bound [NiFe]-hydrogenase of Ralstonia eutropha H16 is one of the few...
Article
[NiFe]-hydrogenases catalyze the oxidation of H(2) to protons and electrons. This reversible reaction is based on a complex interplay of metal cofactors including the Ni-Fe active site and several [Fe-S] clusters. H(2) catalysis of most [NiFe]-hydrogenases is sensitive to dioxygen. However, some bacteria contain hydrogenases that activate H(2) even...

Network

Cited By

Projects

Projects (6)
Project
In this EU project we strive for a synthetic biology approach to specifically produce flavonoids in bacteria.
Project
Sulfurospirillum spp. are free-living Epsilonproteobacteria of environmental (tetrachloroethene, arsenate, selenate respiration) and biotechnological (inhabitants of bioelectrodes, wastewater plants) importance. Here, several new traits of these facultative anaerobes are studied.