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Citations since 2017
6 Research Items
Leaving academic research and embarking on a new career in industry was and still is challenging! I would like to thank all mentors in academia and at my new company for their support along the way! In my new position at Cytosurge, I am combining my skills in molecular cell biology, stem cells and developmental biology with the cutting-edge technology of Cytosurge. We are developing novel applications for the FluidFM Bioseries. In particular, we are interested in cell line engineering.
March 2020 - March 2020
- Senior Researcher
- I am combining my previous knowlede in molecular cell biology, stem cells and genomengineering with the expertise in nanotechnology of Cytosurge. We are aiming to use FLUID FM microscopy to provide solutions to problems in modern life sciences.
October 2018 - September 2019
ETH Zurich Laboratory of Jacob E. Corn
- Senior Researcher
The liver is frequently challenged by surgery-induced metabolic overload, viruses or toxins, which induce the formation of reactive oxygen species. To determine the effect of oxidative stress on liver regeneration and to identify the underlying signaling pathways, we studied liver repair in mice lacking the Nrf2 transcription factor. In these anima...
Somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) by expression of defined embryonic factors. However, little is known of the molecular mechanisms underlying the reprogramming process. Here we explore somatic cell reprogramming by exploiting a secondary mouse embryonic fibroblast model that forms iPSCs with high efficiency...
A small toolkit of morphogens is used repeatedly to direct development, raising the question of how context dictates interpretation of the same cue. One example is the transforming growth factor β (TGF-β) pathway that in human embryonic stem cells fulfills two opposite functions: pluripotency maintenance and mesendoderm (ME) specification. Using pr...
Modifications by kinases are a fast and reversible mechanism to diversify the function of the targeted proteins. The OCT4 transcription factor is essential for preimplantation development and pluripotency of embryonic stem cells (ESC) and its activity is tightly regulated by post-transcriptional modifications. Several phosphorylation sites have bee...
The fibroblast growth factor (FGF) and the transforming growth factor-β (TGF-β) pathways are both involved in the maintenance of human embryonic stem cells (hESCs) and regulate the onset of their differentiation. Their converging functions have suggested that these pathways might share a wide range of overlapping targets. Published studies have foc...
RNA sequencing (RNA-seq) has become a standard procedure to investigate transcriptional changes between conditions and is routinely used in research and clinics. While standard differential expression (DE) analysis between two conditions has been extensively studied, and improved over the past decades, RNA-seq time course (TC) DE analysis algorithm...
Human embryonic stem cells hold great promise for future biomedical applications such as disease modeling and regenerative medicine. However, these cells are notoriously difficult to culture and are refractory to common means of genetic manipulation, thereby limiting their range of applications. In this protocol, we present an easy and robust metho...
Background: Members of the Transforming Growth Factor-beta (TGFβ) superfamily of cytokines are essential for early embryonic development and play crucial roles in pluripotency and differentiation of embryonic stem cells in vitro. Scope of review: In this review, we discuss how TGFβ family signals are read by cells and how they are modulated by t...
The liver is frequently exposed to insults, including toxic chemicals and alcohol, viral infection or metabolic overload. Although it can fully regenerate after acute injury, chronic liver damage causes liver fibrosis and cirrhosis, which can result in complete liver failure. In this study, we demonstrate that the NF-E2-related factor 2 (Nrf2) tran...
The Nrf2 transcription factor is a crucial regulator of the cellular redox homeostasis through its capacity to induce the expression of enzymes, which detoxify reactive oxygen species, and of other antioxidant proteins. Therefore, it plays an important role in the protection from carcinogenesis induced by various insults. In addition, recent result...
The Nrf2 transcription factor plays a key role in the cellular defense against oxidative and xenobiotic stresses through its capability to induce the expression of genes, which encode detoxifying enzymes and antioxidant proteins. Most interestingly, recent studies provide evidence for an important function of Nrf2 in the protection against various...
The NF-E2-related factor 2 (Nrf2) transcription factor is a potent inducer of cytoprotective genes, which encode--among others--enzymes that detoxify reactive oxygen species (ROS). As we demonstrated a crucial role of Nrf2 in the prevention of skin carcinogenesis, it is of interest to identify Nrf2-activating factors in keratinocytes. For this purp...
The Nrf2 transcription factor is a key player in the cellular stress response through its regulation of cytoprotective genes. In this study we determined the role of Nrf2-mediated gene expression in keratinocytes for skin development, wound repair, and skin carcinogenesis. To overcome compensation by the related Nrf1 and Nrf3 proteins, we expressed...
Alternative splicing in the extracellular domain is a characteristic feature of members of the fibroblast growth factor receptor (FGFR) family. This splicing event generates receptor variants, which differ in their ligand binding specificities. A poorly characterized splice variant is FGFR1-IIIb, recently found to be a functional FGF receptor predo...
We have isolated, using RT-PCR, a cDNA from mouse skin wounds that encodes fibroblast growth factor (FGF) 22, a recently discovered member of the FGF family, which is closely related to FGF-7 and FGF-10. Transient expression of tagged FGF-22 protein in COS-1 and MCF-7 cells revealed that the protein was present within the cell and at the cell surfa...
We are looking for challenges to test our superfast FluidFM cell editing technology (https://www.cytosurge.com/applications/crispr-cell-line-development) to generate CRISPR-edited monoclonal cell lines. Stop wasting your time to generate your cellular models but use them to advance your research projects! By directly injecting RNP complexes to the nucleus of single cells, we can edit in principle any adherent cell line with higher efficiency, speed and no detectable off target editing!
So far, we were able to edit:
- Tert immortalized primary epithelial cells in as little as 4 weeks (from single cell to a a monoclonal cell line bearing the desired mutation)
- Triple KO in CHO cells in less than 2 weeks
- HDR rates in cancerous cells 5-10x higher than using classical approaches
What are your difficulties that hamper your cell line engineering projects?
Is there a cell line you would like us to try out for you?
Please contact us: