Tiziana Cotrufo

Tiziana Cotrufo
University of Barcelona | UB · Department of Cell Biology, Physiology and Immunology

Phd in Neurobiology

About

34
Publications
2,884
Reads
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374
Citations
Citations since 2016
17 Research Items
210 Citations
2016201720182019202020212022010203040
2016201720182019202020212022010203040
2016201720182019202020212022010203040
2016201720182019202020212022010203040
Additional affiliations
January 2011 - present
Universitat de Barcelona
January 2003 - present
Scuola Normale Superiore di Pisa

Publications

Publications (34)
Preprint
Full-text available
Brain connectivity requires correct axonal guidance to drive axons to their appropriate targets. This process is orchestrated by guidance cues that exert attraction or repulsion to developing axons. However, the intricacies of the cellular machinery responsible for the correct response of growth cones are just being unveiled. Netrin-1 is a bifuncti...
Article
Full-text available
The metallic impregnation invented by Camillo Golgi in 1873 has allowed the visualization of individual neurons in their entirety, leading to a breakthrough in the knowledge on the structure of the nervous system. Professor of Histology and of General Pathology, Golgi worked for decades at the University of Pavia, leading a very active laboratory....
Data
This animation shows a representative field of view from the cerebral cortex of the rabbit brain, impregnated by the Golgi method. At the chosen X-Y location, 250 still images were acquired with a digital camera (see Methods), focusing the 40X objective at 0.3 μm steps along the z axis. Image acquisition started approximately 30–40 μm below the sur...
Article
Full-text available
SNARE proteins are essential components of the machinery that regulates vesicle trafficking and exocytosis. Their role is critical for the membrane-fusion processes that occur during neurotransmitter release. However, research in the last decade has also unraveled the relevance of these proteins in membrane expansion and cytoskeletal rearrangements...
Article
Full-text available
Axonal growth and guidance rely on correct growth cone responses to guidance cues. Unlike the signaling cascades that link axonal growth to cytoskeletal dynamics, little is known about the crosstalk mechanisms between guidance and membrane dynamics and turnover. Recent studies indicate that whereas axonal attraction requires exocytosis, chemorepuls...
Data
Robo3 surface expression in STX1A/B knock-out commissural neurons. Commissural neurons from E11 wild-type (A, B) and STX1A/B null mutant embryos were cultured (C, D) and incubated with recombinant Netrin-1 (B,D) or with control medium (A,C). Cultures were immunostained for the labeling of surface Robo3 receptor (green color) and the intracellular p...
Data
Diagram of the generation of STX1B mutant mice. (A) Illustration of mutation in the STX1B is shown. The insertion is in the first intron of the gene. (B) Agarose gel illustrating genotyping bands for wt, heterozygous, and STX1B KO mice. (C) Western blot showing the absence of Syntaxin 1B protein in STX1B mutant mice but not in wt or heterozygous mi...
Data
STX1A/B double mutant embryos show aberrant Robo3-immunolabeled commissural axons. E12 mouse spinal cords were immunostained with α-Robo3 antibodies to detect commissural axons; sections were counterstained with DAPI. Low magnification views (A,C) and enlarged views (B,D) are shown. In wt embryos Robo3-stained fibers form a tight bundle of axons di...
Article
Full-text available
Through complex mechanisms that guide axons to the appropriate routes towards their targets, axonal growth and guidance lead to neuronal system formation. These mechanisms establish the synaptic circuitry necessary for the optimal performance of the nervous system in all organisms. Damage to these networks can be repaired by neuroregenerative proce...
Article
Full-text available
Neuron populations of the lateral hypothalamus which synthesize the orexin (OX)/hypocretin or melanin-concentrating hormone (MCH) peptides play crucial, reciprocal roles in regulating wake stability and sleep. The disease human African trypanosomiasis (HAT), also called sleeping sickness, caused by extracellular Trypanosoma brucei (T. b.) parasites...
Article
Full-text available
BACKGROUND Heart transplantation is a therapeutic option for patients with severe coronary artery disease or heart failure. One of the difficulties to overcome is the apoptosis of cardiomyocytes in the donor organ. To prevent apoptosis in the donor organ, we developed a fusion protein containing FLIP (FADD-like interleukin beta-converting enzyme (F...
Article
Axonal growth and guidance rely on correct growth cone responses to guidance cues, both in the central nervous system (CNS) and in the periphery. Unlike the signaling cascades that link axonal growth to cytoskeletal dynamics, little is known about the crosstalk mechanisms between guidance and membrane dynamics and turnover in the axon. Our studies...
Article
Full-text available
Axonal guidance and synaptic specification depends on specific signaling mechanisms that occur in growth cones. While several signaling pathways implicated in cone navigation have been identified, membrane dynamics in growth cones remains largely unknown. We took advantage of SynaptopHluorin and high-speed optical recordings to monitor the patterns...
Article
Full-text available
Glioblastoma (GBM) is the most prevalent adult brain tumor, with virtually no cure, and with a median overall survival of 15 months from diagnosis despite of the treatment. SNARE proteins mediate membrane fusion events in cells and are essential for many cellular processes including exocytosis and neurotransmission, intracellular trafficking and ce...
Article
Full-text available
Neurotrophins are involved in many crucial cellular functions, including neurite outgrowth, synapse formation, and plasticity. Although these events have long been known, the molecular determinants underlying neuritogenesis have not been fully characterized. Ack1 (activated Cdc42-associated tyrosine kinase) is a non-receptor tyrosine kinase that is...
Article
Directed cell migration and axonal guidance are essential steps in neural development that share many molecular mechanisms. The guidance of developing axons and migrating neurons is likely to depend on the precise control of plasmalemma turnover in selected regions of leading edges and growth cones, respectively. Previous results provided evidence...
Article
Full-text available
Directed cell migration and axonal guidance are essential steps in neural development. Both processes are controlled by specific guidance cues that activate the signaling cascades that ultimately control cytoskeletal dynamics. Another essential step in migration and axonal guidance is the regulation of plasmalemma turnover and exocytosis in leading...
Article
Reelin is a glycoprotein that modulates synaptic function and plasticity in the mature brain, thereby favouring memory formation. We recently reported altered cerebral Reelin expression in Alzheimer's disease (AD). Here we demonstrate pronounced Reelin changes at protein and mRNA levels in the frontal cortex in adult Down's syndrome (DS), where the...
Article
Full-text available
Total lack of visual experience [dark rearing (DR)] is known to prolong the critical period and delay development of sensory functions in mammalian visual cortex. Recent results show that neurotrophins (NTs) counteract the effects of DR on functional properties of visual cortical cells and exert a strong control on critical period duration. NTs are...
Article
Total lack of visual experience (dark rearing, DR) is known to affect development of mammalian visual cortex (VC) and to prolong the critical period of visual cortical plasticity. Neurotrophins (NTs) have been proposed to play a relevant role in activity dependent processes important for the final shaping of cortical visual connections. Neurotrophi...
Article
The purpose of this study was to determine the mechanism by which inosine activates pyrimidine salvage in CNS. The levels of cerebral inosine, hypoxanthine, uridine, uracil, ribose 1-phosphate and inorganic phosphate were determined, to evaluate the Gibbs free energy changes (deltaG) of the reactions catalyzed by purine nucleoside phosphorylase and...

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