Tim Ross-Elliott

Tim Ross-Elliott
Harvard Medical School | HMS

PhD - Molecular Plant Sciences

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14
Publications
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397
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Publications

Publications (14)
Preprint
Biased signaling occurs when different ligands that are directed at the same receptor launch different cellular outcomes. Because of their pharmacological importance, we know the most about biased ligands and little is known about other mechanisms to achieve signaling bias. In the canonical animal G protein system, endocytosis of a 7-transmembrane...
Article
Contents I II. III. IV. V. VI. References WITH NO LYSINE (WNK) kinases are serine/threonine kinases uniquely characterized by an anomalous placement of a catalytic lysine, hence their moniker. In animals, WNK protein kinases play critical roles in protein trafficking of components that mediate renal ion transport processes and regulate osmoregulati...
Article
Full-text available
Differentiating sieve elements in the phloem of angiosperms produce abundant phloem-specific proteins before their protein synthesis machinery is degraded. These P-proteins initially form dense bodies, which disperse into individual filaments when the sieve element matures. In some cases, however, the dense protein agglomerations remain intact and...
Data
Examples of non-dispersive P-protein bodies (NPBs) that do not respond to wounding of sieve elements by puncturing with micro-pipettes. The CLSM micrographs were taken after a sieve element had been severed with a micro-pipette; arrowheads point to unresponsive NPBs. In all cases, the NPBs are located close to a sieve plate (compare Fig. 5). (A) Th...
Data
Comparison of the putative promoter sequences of the P. trichocarpa genes Potri.001G430200.1 and Potri.017G071000.1, the PtSEOR1 gene. Identical bases appear in blue, different bases and gaps are shown in red. The 100-bp sequence that confers phloem-specificity to the Potri.001G340200.1 promoter (Nguyen et al., 2017) and the corres-ponding sequence...
Data
Comparison of the hypothetical product of gene Potri.017G071000.1 (from Populus trichocarpa v3.0, https://phytozome.jgi.doe.gov/pz/portal.html), PtSEOR1, and AtSEOR1. Positions in which Potri.017G071000.1 and PtSEOR1 differ show in red, residues that are identical in the two are shown in blue. Positions in which AtSEOR1 matches the consensus of the...
Data
TEM micrographs of a sieve element in an Arabidopsis leaf with bundles of SEOR protein filaments in longitudinal and perpendicular section. (A) cross-section of a sieve element with two slime masses consisting of filaments of SEOR protein (arrows). (B) zoom into one of the protein masses, showing longitudinal or oblique sections of the winding SEOR...
Data
Amino acid sequences of the proteins analyzed in Fig. 7.
Article
Sieve elements (SEs) degrade selected organelles and cytoplasmic structures when they differentiate. According to classical investigations, only smooth ER, mitochondria, sieve element plastids, and, in most cases, P-proteins remain in mature SEs. More recent proteomics and immuno-histochemical studies, however, suggested that additional components...
Article
Full-text available
In plant cells, heterotrimeric G protein signaling mediates development, biotic/abiotic stress responsiveness, hormone signaling, and extracellular sugar sensing. The amount of sugar in plant cells fluctuates from nanomolar to high millimolar concentrations over time depending on changes in the light environment. Arabidopsis thaliana Regulator of G...
Article
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In plants, a complex mixture of solutes and macromolecules is transported by the phloem. Here, we examined how solutes and macromolecules are separated when they exit the phloem during the unloading process. We used a combination of approaches (non-invasive imaging, 3D-electron microscopy, and mathematical modelling) to show that phloem unloading o...
Article
Full-text available
Investigation of Structure-Function Relationship of Long-Distance Transport in Plants: New Imaging Tools to Answer Old Questions - Volume 21 Issue S3 - Daniel L Mullendore, Daniel R Froelich, Sierra Beecher, Tim J Ross-Elliott, Jan Knoblauch, Michael Knoblauch
Article
Full-text available
Using Arabidopsis seedlings, we identified a range of small fluorescent probes that entered the translocation stream and were unloaded at the root tip. These probes had absorbance/emission maxima ranging from 367/454 nm to 546/576 nm and represent a versatile toolbox for studying phloem transport. Of the probes we tested, naturally occurring fluore...
Article
Full-text available
Since the first ultrastructural investigations of sieve tubes in the early 1960s, their structure has been a matter of debate. Because sieve tube structure defines frictional interactions in the tube system, the presence of P protein obstructions shown in many transmission electron micrographs led to a discussion about the mode of phloem transport....

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