Tilman Lamparter

Tilman Lamparter
Karlsruhe Institute of Technology | KIT · Botanical Institute

Prof. Dr. rer. nat.

About

177
Publications
29,180
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
4,448
Citations
Introduction
Tilman Lamparter works at the Botanical institute of the KIT. His major interest is in phytochrome, photolyases, photobiology and on biotechnological applications with microalgae. Keywords for search engines: Phytochromes Photolyases Agrobacterium fabrum Biochemistry Recombinant expression of proteins Use of the filamentous cyanobacterium Phormidium lacuna HE10JO and other novel strains from the North Sea and the Mediterranean Sea for biotechnology
Additional affiliations
April 2007 - present
Karlsruhe Institute of Technology
Position
  • Professor (Full)
January 2004 - February 2004
University of Leeds
Position
  • guest scientist
January 1990 - May 2007
Freie Universität Berlin
Position
  • Post Doc / Oberassistent
Description
  • Course Molecular Biology 10 SWS every 2nd semester Lecture Plant Physiology 2 SWS every 2nd semester Course Evelopmental Biology 5 SWS every 2nd semester

Publications

Publications (177)
Article
Full-text available
Evidence that archaeplastidal phytochromes originate from cyanobacteria, based on chromophore interacting amino acids
Article
Phytochromes are photoreceptor proteins with a bilin chromophore that undergo photoconversion between two spectrally different forms, Pr and Pfr. In plants, phytochromes play a central role in growth and differentiation during the entire life cycle. Phytochromes of plants and other groups of archaeplastida have a common evolutionary origin in proka...
Article
Full-text available
Phormidium lacuna is a naturally competent, filamentous cyanobacterium that belongs to the order Oscillatoriales. The filaments are motile on agar and other surfaces and display rapid lateral movements in liquid culture. Furthermore, they exhibit a photophobotactic response, a phototactic response towards light that is projected vertically onto the...
Article
Full-text available
Mitochondria are essential organelles because of their function in energy conservation. Here, we show an involvement of mitochondria in phytochrome-dependent light sensing in fungi. Phytochrome photoreceptors are found in plants, bacteria, and fungi and contain a linear, heme-derived tetrapyrrole as chromophore. Linearization of heme requires heme...
Article
Full-text available
The soil bacterium and plant pathogen Agrobacterium fabrum C58 has two phytochrome photoreceptors, Agp1 and Agp2. We found that plant infection and tumor induction by A. fabrum is down-regulated by light and that phytochrome knockout mutants of A. fabrum have diminished infection rates. The regulation pattern of infection matches with that of bacte...
Article
Full-text available
Deactivation processes of photoexcited (λ ex = 580 nm) phycocyanobilin (PCB) in methanol were investigated by means of UV/Vis and mid-IR femtosecond (fs) transient absorption (TA) as well as static fluorescence spectroscopy, supported by density-functional-theory calculations of three relevant ground state conformers, PCB A , PCB B and PCB C , thei...
Preprint
Full-text available
Phormidium lacuna , a filamentous cyanobacterium without heterocysts, can be transformed by natural transformation. These filaments are motile on agar and other surfaces and display rapid lateral movements in liquid culture. Furthermore, they exhibit phototactic response under vertical illumination in Petri dishes. We generated mutants in which a K...
Article
Full-text available
The focus of this review is on the phytochromes Agp1 and Agp2 of Agrobacterium fabrum. These are involved in regulation of conjugation, gene transfer into plants and other effects. Since crystal structures of both phytochromes are known, the phytochrome system of A. fabrum provides a tool for following the entire signal transduction cascade startin...
Article
Full-text available
Photolyases are proteins that enzymatically repair the UV-induced DNA damage by a protein-DNA electron transfer mechanism. They repair either cyclobutane pyrimidine dimers or pyrimidine (6-4) pyrimidone photoproducts or just (6-4)-photoproducts. In this work, we report the production and partial characterization of a recombinant (6-4)-photolyase (S...
Article
Full-text available
Photolyases are flavoproteins that repair ultraviolet-induced DNA lesions (cyclobutane pyrimidine dimer or CPD, and pyrimidine (6-4) pyrimidone photoproducts or (6-4)-PPs), using blue light as an energy source. These enzymes are substrate specific, meaning that a specific photolyase repairs either a CPD or a (6-4)-PP. In this work, we produced a cl...
Article
Full-text available
Research for biotechnological applications of cyanobacteria focuses on synthetic pathways and bioreactor design, while little effort is devoted to introduce new, promising organisms in the field. Applications are most often based on recombinant work, and the establishment of transformation can be a risky, time-consuming procedure. In this work we d...
Preprint
Full-text available
The soil bacterium Agrobacterium fabrum C58 infects plants by a unique DNA transfer mechanism. A. fabrum has two phytochrome photoreceptors, Agp1 and Agp2. We found that DNA transfer into plants by A. fabrum is down regulated by light and that phytochrome knockout mutants have diminished DNA transfer rates. The regulation pattern matches with that...
Preprint
Full-text available
Research for biotechnological applications of cyanobacteria focuses on synthetic pathways and bioreactor design, while little effort is devoted to introduce new, promising organisms in the field. Applications are most often based on recombinant work, and the establishment of transformation can be a risky, time-consuming procedure. In this work we d...
Article
Full-text available
The adaptation of microorganisms to different temperatures is an advantage in habitats with steadily changing conditions and raises the question about temperature sensing. Here we show that in the filamentous fungus Aspergillus nidulans the hybrid histidine kinase TcsB and phytochrome are involved in temperature‐induced gene transcription. Temperat...
Conference Paper
Agrobacterium fabrum is non-photosynthetic, gram-negative, rod-shaped soil bacterium that causes crown gall (tumor) formation in at least 140 species of dicotyledons or gymnosperms. Tumor formation is initiated by a transfer of T-DNA from Ti plasmid of the bacterium into the genome of the plant cell. Agrobacterium fabrum type IV secretion system (T...
Article
Full-text available
The repair of sun-induced DNA lesions by photolyases is driven by a photoinduced electron transfer from a fully reduced FAD to the damaged DNA. A chain of several aromatic residues connecting FAD to solvent ensures the prior photoreduction of the FAD cofactor. In PhrA, a class III CPD photolyase, two branching tryptophan charge transfer pathways ha...
Article
Full-text available
During bacterial conjugation, plasmid DNA is transferred from cell to cell. In Agrobacterium fabrum, conjugation is regulated by the phytochrome photoreceptors Agp1 and Agp2. Both contribute equally to this regulation. Agp1 and Agp2 are histidine kinases, but, for Agp2, we found no autophosphorylation activity. A clear autophosphorylation signal, h...
Preprint
In PhrA, a class III CPD photolyase, two branching tryptophan charge transfer pathways have been characterized in the mechanism of FAD photoreduction. To provide a molecular explanation of the charge transfer abilities of both pathways, we performed simulations where the protein motion and the positive charge are simultaneously propagated. Our comp...
Chapter
Bakterien sind die Modellorganismen schlechthin. Viele Bakterien können gut in Kultur gehalten werden, sie wachsen schnell und es ist einfach, genetische Experimente zu machen. Fast alle Gene und Proteine eukaryotischer Organismuseukaryotischer haben ein prokaryotisches Homologes, für viele eukaryotische Proteine wurden bakterielle Homologe untersu...
Article
Full-text available
Prokaryotic (6‐4) photolyases branch at the base of the evolution of cryptochromes and photolyases. Prototypical members contain an iron‐sulphur cluster which was lost in the evolution of the other groups. In the Agrobacterium (6‐4) photolyase PhrB, the repair of DNA lesions containing UV‐induced (6‐4) pyrimidine dimers is stimulated by Mg2+. We pr...
Article
Full-text available
Photolyases are DNA-repairing flavoproteins that are represented in most phylogenetic taxa with the exception of placental mammals. These enzymes reduce the ultraviolet-induced DNA damage; thus, they have features that make them very attractive for dermatological or other medical uses, such as the prevention of human skin cancer and actinic keratos...
Article
Dieses Buch vermittelt ein Verständnis dafür, was Modellorganismen sind, und wofür sie in der Wissenschaft eingesetzt werden. Im Zentrum steht, welche grundsätzlichen Ansätze mit Modellorganismen verfolgt werden und welche Limitierungen man beachten muss. Die wichtigsten Modellorganismen werden anhand von übersichtlichen Steckbriefen vorgestellt un...
Article
Full-text available
Phytochromes are modular photoreceptors of plants, bacteria and fungi that use light as a source of information to regulate fundamental physiological processes. Interconversion between the active and inactive states is accomplished by a photoinduced reaction sequence which couples the sensor with the output module. However, the underlying molecular...
Article
How the environment shapes the function and evolution of DNA repair systems is poorly understood. In a comparative study using zebrafish and the Somalian blind cavefish, Phreatichthys andruzzii, we reveal that during evolution for millions of years in continuous darkness, photoreactivation DNA repair function has been lost in P. andruzzii. We demon...
Preprint
Full-text available
Prokaryotic (6-4) photolyases branch at the base of the evolution of cryptochromes and photolyases. In the Agrobacterium (6-4) photolyase PhrB, the repair of DNA with UV-induced (6-4) pyrimidin dimers is stimulated by Mg2+. We show that Mg2+ is required for efficient lesion binding and for charge stabilization after electron transfer from the FADH-...
Technical Report
Full-text available
The goal of this project was to create an action spectrum of the chlorophyll synthesis of sunflower seedlings using 3D printed diode mountings. In the first part of the project different types of illumination units were build together. For the action spectrum we choose diodes emitting monocromatic light in following wavelengths: 400, 458, 470, 505,...
Article
In biotechnological applications, cyanobacteria are employed for conversion of CO2 into bioproducts with sunlight as sole energy source. We describe the isolation of motile filamentous cyanobacteria from rockpools of the North Sea or the Mediterranean Sea and their characterization by physiological assays and genome sequencing. The five isolated li...
Article
Full-text available
Bacterial phytochromes are dimeric light-regulated histidine kinases that convert red light into signaling events. Light absorption by the N-terminal photosensory core module (PCM) causes the proteins to switch between two spectrally distinct forms, Pr and Pfr, thus resulting in a conformational change that modulates the C-terminal histidine kinase...
Article
Full-text available
The (6-4) photolyases of the FeS-BCP group can be considered as the most ancient type among the large family of cryptochrome and photolyase flavoproteins. In contrast to other photolyases they contain an Fe-S cluster of unknown function, a DMRL chromophore, an interdomain loop which could interact with DNA, and a long C-terminal extension. We compa...
Article
Full-text available
PhrB from Agrobacterium fabrum is the first prokaryotic photolyase which repairs (6-4) UV DNA-photoproducts. The protein harbors three cofactors, the enzymatically active FAD chromophore, a second chromophore, 6,7-dimethyl-8-ribityllumazine (DMRL) and a cubane-type Fe-S cluster. Tyr424 of PhrB is part of the DNA binding site and could provide an el...
Article
Full-text available
Agp1 is a canonical biliverdin-binding bacteriophytochrome from the soil bacterium Agrobacterium fabrum that acts as a light-regulated histidine kinase. Crystal structures of the photosensory core modules (PCMs) of homologous phytochromes have provided a consistent picture of the structural changes that these proteins undergo during photoconversion...
Article
Bacterial conjugation, the transfer of single stranded plasmid DNA from donor to recipient cell, is mediated through the type IV secretion system. We performed conjugation assays using a transmissible artificial plasmid as reporter. With this assay, conjugation in Agrobacterium fabrum was modulated by the phytochromes Agp1 and Agp2, photoreceptors...
Article
Bathy phytochrome Agp2 from Agrobacterium fabrum exhibits an unusually low pKa =7.6 in the Pr state in contrast to a pKa >11 in the Pfr state, indicating a pH-dependent charge distribution and H-bond network in the Pr chromophore binding pocket around neutral pH. Here, we report on ultrafast UV/Vis absorption spectroscopy of the primary Pr photoiso...
Article
Phytochromes are widely distributed photoreceptors with similar domain arrangements. The evolutionary origin of plant and green algal phytochromes is currently under debate. We used different algorithms to generate multiple phylogenetic trees for the N-terminal chromophore module and C-terminal the histidine kinase domains. Evolution of the chromop...
Article
Full-text available
Photolyases can repair pyrimidine dimers on the DNA that are formed during UV irradiation. PhrB from Agrobacterium fabrum represents a new group of prokaryotic (6-4) photolyases which contain an iron-sulfur cluster and a DMRL chromophore. We performed site-directed mutagenesis in order to assess the role of particular amino acid residues in photore...
Article
The phytochrome family of light-switchable proteins has long been studied by biochemical, spectroscopic and crystallographic means, while a direct probe for global conformational signal propagation has been lacking. Using solution X-ray scattering, we find that the photosensory cores of several bacterial phytochromes undergo similar large-scale str...
Article
Full-text available
Phytochromes are biological photoreceptors that can be reversibly photoconverted between a dark and photoactivated state. The underlying reaction sequences are initiated by the photoisomerization of the tetrapyrrole cofactor, which in plant and cyanobacterial phytochromes are a phytochromobilin (PΦB) and a phycocyanobilin (PCB), respectively. The t...
Article
Full-text available
Phytochromes are red light-sensitive photoreceptors that control a variety of developmental processes in plants, algae, bacteria and fungi. Prototypical phytochromes exhibit an N-terminal tridomain (PGP) consisting of PAS, GAF and PHY domains and a C-terminal histidine kinase (HK). The mode of evolution of streptophyte, fungal and diatom phytochrom...
Article
Full-text available
Photolyases are proteins with an FAD chromophore that repair UV-induced pyrimidine dimers on the DNA in a light dependent manner. The cyclobutane pyrimidine dimer (CPD) class III photolyases are structurally unknown but closely related to plant cryptochromes, which serve as blue-light photoreceptors. Here we present the crystal structure of a class...
Article
Full-text available
Bacteriophytochromes (BphPs) are light-sensing regulatory proteins encoded in photosynthetic and non-photosynthetic bacteria. This protein class incorporate bilin as their chromophore, with majority of them bearing a light- regulated His kinase or His kinase related module in the C-terminal. We studied the His kinase actives in the temperature rang...
Chapter
Full-text available
Recently, a new phylogenetic group of the photolyase cryptochrome family termed iron–sulfur bacterial cryptochromes/photolyases (FeS-BCP) was discovered. Two members of this group, CryB from Rhodobacter sphaeroides and ‘photolyase related protein’ (PhrB) from Agrobacterium tumefaciens, carry a new antenna chromophore, 6,7-dimethyl 8- ribityl-lumazi...
Article
Fluorescence line narrowing (FLN) spectroscopy was used to study bacteriophytochromes and variants from various species in their red absorbing Pr ground state, including phytochromes Agp1 from Agrobacterium tumefaciens, DrBphP from Deinococcus radiodurans and RpBphP2 and RpBphP3 from Rhodops