Tiina Karu

Tiina Karu
Laser Technology Research Center of Russian Academy of Science, Moscow, Russia · Laboratory of Laser Biomedicine

Dr.Sci., biophysics

About

191
Publications
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Introduction
Additional affiliations
January 1979 - April 2016
Laser Technology Research Center of Russian Academy of Science, Moscow, Russia
Position
  • Head of the laboratory

Publications

Publications (191)
Chapter
Various cellular responses to visible and infrared A (IR-A) radiation have been studied for decades in the context of molecular mechanisms of laser low level light therapy (LLLT). LLLT uses monochromatic and quasi-monochromatic light in the optical region of 600–1000 nm to treat in a non-destructive and non-thermal fashion various soft tissue and n...
Article
Monochromatic (laser) and quasimonochromatic (LED) radiation in the red-to-near infrared optical region both in CW and pulsed modes is used to treat in a nondestructive fashion various soft-tissue and neurologic conditions. Cytochrome c oxidase, the terminal enzyme of mitochondrial respiratory chain, is considered as the photoacceptor. The mechanis...
Article
The question of whether photobiomodulation should be used as a drug equivalent arose in my mind after listening to presentations at the recent conference of the World Association for Laser Therapy (WALT)-2012 (Gold Cost City, Australia), and later at home when searching MEDLINE® for the years 2009–2012. Photobiomodulation (earlier terms: low level...
Article
The mitochondrial retrograde signaling is an information channel between mitochondrial respiratory chain and the nucleus for the transduction signals regarding the functional state of the mitochondria. The present review examines the question whether radiation of visible and near IR (IR-A) radiation can activate this cellular signaling pathway. The...
Article
Experimental data about the modulation of adhesion and proliferation of anchorage-dependent HeLa cells with monochromatic or quasimonochromatic radiation in red to near-infrared region are presented. Cell adhesion and proliferation can be increased by irradiation with light of certain wavelengths (maxima in action spectrum are 619, 675, 740, 760, a...
Article
This article reviews the current knowledge in photobiology and photomedicine about the influence of monochromatic, quasimonochromatic, and bread-band radiation of red-to-near infrared (IR-A) part on solar spectrum upon mammalian cells and human skin. The role of cytochrome c oxidase as the photoacceptor and photosignal transducer is underlined and...
Article
Full-text available
It was shown that, 1 h after irradiation of human blood lymphocytes with a He-Ne laser at 56 J/m2 (5.6 W/m2, 10 sec), the relative optical density of condensed chromatin masses observed in ultrathin sections was decreased (p < 0.01); i.e., the condensed chromatin became less compact. Such transition of condensed chromatin to a more “open” state may...
Article
Full-text available
The objective of this work was a further investigation of redox mechanisms of laser phototherapy on the cellular level. Cytochrome c oxidase, the terminal enzyme of the mitochondrial respiratory chain, is believed to work as the photoacceptor to modulate cellular metabolism in laser phototherapy. The changes in the absorption spectra of HeLa-cell m...
Article
Mitochondrial signaling is an information channel between the mitochondrial respiratory chain and the nucleus for the transduction signals regarding the functional state of the mitochondria. The present review examines the question whether radiation of visible and near-IR (IR-A) radiation can activate this retrograde-type cellular signaling pathway...
Article
Full-text available
The objective of this work was to evaluate the importance of the degree of light polarization in stimulation of cellular metabolism. Although the possible role of polarization's effects on the mechanisms of laser phototherapy is sometimes discussed in the literature, there are still no clear answers. A model system (HeLa cell suspension) was used i...
Article
An investigation was made of the effects of radiation from a pulsed gallium arsenide (λ = 890 nm, pulse repetition frequency 666 and 3480 Hz, intensity 12 mW/m2) on the rate of growth of E. coli bacteria. It was found that the growth of a culture was accelerated by radiation doses 0.1–1.5 J/m2 and the maximum effect (when ratio of the number of cel...
Article
An investigation was made of the selective action of pulsed laser radiation (fourth harmonic of a YAG:Nd3+ laser having a wavelength of 266 nm and a pulse duration of 35 psec) on nucleic acids in HeLa tumor cells which were at the logarithmic stage of growth. It was found that by varying the radiation parameters (number of pulses and their intensit...
Article
An investigation was made of the action of low-intensity cw visible light of wavelengths in the range 313–860 nm on the rate of DNA synthesis in a culture of HeLa cells. It was found that the synthesis of DNA was stimulated in three spectral ranges: 313–450 nm, 600–650 nm, and 750–840 nm. For a fixed dose the stimulation effect was very sensitive t...
Article
An investigation was made of the action of pulsed laser radiation (λ = 578.2 nm copper vapor laser) on nucleic acids in HeLa cells at the proliferating or quiescent stages. It is found that RNA synthesis is sensitive to irradiation in both models but the character of the dose–effect dependence is the opposite. In the case of proliferating cells, do...
Article
An investigation was made of the action of λ = 633 nm low-intensity red light (He–Ne laser, incandescent lamp with light filters, dye laser pumped by a copper laser) on the rate of nucleic acid synthesis in HeLa cells 1.5 h after irradiation. It was found that DNA synthesis is stimulated almost equally by irradiation with an He–Ne laser and with a...
Article
Phototherapy uses monochromatic light in the optical region of 600-1000 nm to treat in a non-destructive and non-thermal fashion various soft-tissue and neurological conditions. This kind of treatment is based on the ability of light red-to-near IR to alter cellular metabolism as a result of its being absorbed by cytochrome c oxidase. To further in...
Article
The data on the effect of He-Ne laser light (lambda = 632.8 nm) on mitochondria of yeasts in late log phase were reviewed. The qualitative analysis of the ultrathin sections of cells demonstrated a nonuniform thickness of the giant branched mitochondria typical for budding yeasts. Exposure to a dose of 460 J/m@2 accelerated cell proliferation, acti...
Article
Full-text available
The aim of the present work is to analyze available action spectra for various biological responses of HeLa cells irradiated with monochromatic light of 580-860 nm. Phototherapy (low-level laser therapy or photobiomodulation) is characterized by its ability to induce photobiological processes in cells. Exact action spectra are needed for determinat...
Article
The objective of this study was to investigate the possibility of involvement of nitric oxide (NO) into the irradiation-induced increase of cell attachment. These experiments were performed with a view to exploring the cellular mechanisms of low-power laser therapy. A suspension of HeLa cells was irradiated with a monochromatic visible-to-near infr...
Article
Full-text available
The data on the aftereffect of He-Ne laser light (λ = 632.8 nm) on mitochondria of yeasts in late log phase were reviewed. The quantitative analysis of the ultrathin cell sections demonstrated a nonuniform thickness of the giant branched mitochondria typical for budding yeasts. Exposure to a dose of 460 J/m2 (accelerating cell proliferation and act...
Article
The number of cells attached to glass substratum increases if HeLa cell suspension is irradiated with monochromatic visible-to-near infrared radiation before plating (the action spectrum with maxima at 619, 657, 675, 700, 740, 760, 800, 820, 840 and 860 nm). Treating of cell suspension with sodium azide (2 x 10(-5) M), sodium nitroprusside (5 x 10(...
Article
Full-text available
The number of cells attached to glass substrates increases if HeLa cell suspensions are irradiated with monochromatic visible-to-near infrared radiation (600-860 nm, 52 J m(-2)) prior to plating. The well-structured relationship between this biological response and the radiation wavelength (action spectrum with maxima at 620, 680, 760, and 820 nm)...
Article
We studied the ultrastructure of Torulopsis sphaerica yeast cells irradiated with He-Ne laser (lambda = 632.8 nm, dose--460 J/m2) and then cultured for 6 h in the nutrient with 1% glucose by aeration. The length of membranes of endoplasmic reticulum (ER) and the number of its associations with mitochondria (M) and plasma membrane (PM) were measured...
Article
Chemiluminescence (CL) of peripheral blood from the same donor was recorded after irradiation with various individual laser and superluminous diodes (660, 820, 880 and 950 nm, pulse repetition rates 16, 292 and 5000 Hz) during two periods of acute viral respiratory illness and in normal conditions of health. It was found that precise and statistica...
Conference Paper
Cytochrome c oxidase is considered as the photoacceptor when eukaryotic cells are exposed to monochromatic red to near-IR radiation. Five primary mechanisms are discussed: changes in the redox properties of the respiratory chain components following photoexcitation of their electronic states, NO release from catalytic center of cytochrome c oxidase...
Article
The adhesion of human cervical cancer (HeLa) cells to a glass matrix is evaluated following their irradiation in a suspension with a pulsed near-infrared (IR) light-emitting diode (wavelength 820 nm, pulse repetition frequency 10 Hz, irradiation dose 16-120 J/m2) when melatonin (4 x 10(-11) to 4 x 10(-5) m) is added to cell suspension immediately b...
Chapter
The first publications about low-power laser therapy (then called laser biostimulation) appeared more than 30 years ago. Since then, approximately 2000 studies have been published on this still controversial topic. In the 1960s and 1970s, doctors in Eastern Europe, and especially in the Soviet Union and Hungary, actively developed laser biostimulat...
Article
A study was made of structural organization of Torulopsis sphaerica cells irradiated with He-Ne (lambda = 632.8 nm; dose--460 J/m2) and then cultured in the nutrient with 1% glucose and O2 for 6 h. The computer analysis of electron images of cell sections was carried out. Evidences of stimulation of cell proliferation were found, including decrease...
Chapter
The most frequently used mechanism of photon energy conversion in laser medicine is heating. Average heating of irradiated samples occurs with all methods of tissue destruction (cutting, vaporization, coagulation, ablation). Many of these surgical laser techniques are reviewed elsewhere in this book.
Article
Full-text available
A sensitive method for measuring the circular dichroism (CD) of living HeLa cells in the visible-near infrared (IR) region is developed. The changes in CD spectra from 250 to 780 nm of HeLa cell suspension after the first and second irradiation at 820 nm in dose 9 J/cm<sup>2 </sup> are investigated. The CD spectrum of the intact cells is well struc...
Article
Full-text available
Redox absorbance changes in living cells (monolayer of HeLa cells) under laser irradiation at 633, 670, and 820 nm have been studied by the method of multichannel recording in spectral range 530-890 nm. It has been found that the irradiation causes changes in the absorption spectrum of the cells in two regions, near 754-795 nm (maxima at 757, 775,...
Conference Paper
A sensitive method for measuring the circular dichroism (CD) of living cells in visible-near IR region is developed. The changes in CD spectra from 250 to 780 nm of HeLa cell suspension after the first and second irradiation at 820 nm in dose 9 J/cm2 are investigated. The CD spectrum of the intact cells is well structured and characterized by a pos...
Article
Adhesive interactions between cells and extracellular matrices play a regulative role in wound repair processes. The objective of this investigation is to study action mechanisms of pulsed radiation at 820 nm on cellular adhesion in vitro. Light emitting diodes (LED) at 820 nm are widely used for treatment of wounds of various etiology. The LED (82...
Article
The adhesion of HeLa cells to a glass matrix was evaluated after the irradiation of the cell suspension with a pulsed near-infrared light-emitting diode (lambda = 820 nm, frequency 10 Hz, dose 8-120 J/m(2)) and treatment with two donors of nitric oxide, sodium nitroprusside (SNP, 5 x 10(-4) M) and NaNO(2) (4 x 10(-4) M). It was found that in an irr...
Article
Recent progress in methods of electron microscopy provided a fundamentally new insight into mechanisms of structural and functional interactions between intracellular membranes. For example, mitochondrial membranes were found to contain specific “bridges” providing integrity of double-membrane systems. It was also shown that certain segments of mit...
Article
Adhesive interactions between cells and extracellular matrices play a regulative role in wound repair processes. The objective of this investigation is to study the mechanisms of light action on cellular adhesion in vitro. The adhesion of HeLa cells to a glass matrix is evaluated after irradiation with a pulsed near-infrared (IR) diode and treatmen...
Article
Based oil the number of cells attached to glass, changes are studied in the adhesive properties of cellular membranes 30 min. after irradiating a HeLa cell suspension with a pulsed GaAIAs laser (λ = 820 nm, dose 60 J/m2, pulse repetition frequency 0.1, 0.2, 0.5, 1.0, 2.5, 10, 50 or 100 Hz, duty factor 5, 10, 20, 40, 70 or 95%). It is demonstrated t...
Article
The amount of ATP in HeLa cells was measured by the bioluminescence technique 20 min after exposure to a semiconductor laser (820 nm, 1 Hz, 40 mW 1 × 103 J/m2, pulse width 950 ms, dark period between pulses 50 ms): It was found that the average amount of ATP was (0.95±0.11) × 10-15 mole/cell in unexposed cells and (0.726±0.069) × 10-15 mole/cell in...
Article
Mechanisms of low-power laser light action on cellular level were discussed. It was shown that coherent effects in the light-cells interaction occured at intensities ≥2×1015. The results showed that biological responses of cells to visible and near-infrared radiation occured due to physical and/or chemical changes in photoacceptor molecules.
Article
The structure of mitochondria of yeast cells Torulopsis sphaerica was studied in cells of 6th to 7th generations after irradiation of initial culture with a He-Ne laser (632.8 nm; 460 J/m2 or 1150 J/m2). Ultrathin sections of cells were studied by electron microscopy (morphometric analysis of random sections and spatial reconstruction of chondriome...
Article
The aim of these experiments was a direct measurement of changes in absorption of cellular monolayers under diode laser irradiation. First, a sensitive method for multichannel registration of absorption of a cell monolayer in range 500-860 nm was developed. Second, absorption spectra of intact HeLa cells and those after irradiation the monolayer wi...
Article
Cytochrome c oxidase is discussed as a possible photoacceptor when cells are irradiated with monochromatic red to near-IR radiation. Four primary action mechanisms are reviewed: changes in the redox properties of the respiratory chain components following photoexcitation of their electronic states, generation of singlet oxygen, localized transient...
Article
The aim of these experiments was a direct measurement of changes in absorption of cellular monolayers under diode laser irradiation. First, a sensitive method for multichannel registration of absorption of a cell monolayer in range 500 - 860 nm was developed. Second, absorption spectra of intact HeLa cells and those after irradiation the monolayer...
Article
Effect of He-Ne laser (λ = 632.8 nm) irradiation on successive generations of Torulopsis sphaerica yeast cells were studied on ultrathin sections by electron microscopy. Morphometric analysis of mitochondria and spatial reconstruction of these organelles using serial sections throughout the whole budding cells were performed. One giant and 2-3 smal...
Article
This paper deals with the cytofluorimetric investigation into an early reaction of T- and B-lymphocytes from human peripheral blood (accessibility of the overall nuclear chromatin to acridine orange) to irradiation with a He-Ne laser (632.8 nm, 67 J/m2, 10 s). Our finding is that it is only the T-lymphocytes that react to the laser treatment, where...
Article
The concept of blood irradiation therapy originated from Germany and the United States in the 1920s. In their studies, devices for extracorporeal irradiation of small amounts of blood with UV radiation were developed. The method was used to treat bacterial, viral and autoimmune diseases but was put to oblivion by the discovery of antibiotics and va...
Conference Paper
The following topics are discussed: quantitative laws of light action; primary mechanisms of light action; secondary reactions; controversies and limitations
Article
The electrophysiological characteristics rat spinal cord neurons, rat hippocampus pyramidal neurons, guinea pig cardiomyocytes, rat brain glial cells, and bovine pulmonary artery endothelial cells were measured by the common patch- clamp technique. No light effects on the voltage-activated whole-cell ionic currents were found in any type of the cel...
Article
The aim of these experiments was a direct measurement of changes in absorption of cellular monolayers under diode laser irradiation. First, a sensitive method for multichannel registration of absorption of a cell monolayer in range 500-860 nm was developed. Second, absorption spectra of intact HeLa cells and those after irradiation the monolayer wi...
Article
The structure of mitochondria was studied after the irradiation of human lymphocytes with an He-Ne laser (wavelength, 632.8 nm; dose, 56 J m-2). Ultrathin sections of the lymphocytes were studied by electron microscopy 1 h after the irradiation. The irradiation resulted in a 20% increase (p = 0.95) in the number of mitochondrial profiles on the cel...
Article
In recent years, clinical low-intensity laser therapy practice has used pulsed radiation, mainly from semiconductor lasers. Experimental works devoted to the study of relationships between biological and clinical effects and parameters of pulsed radiation are practically absent. The radiation source was a laser diode emitting at 820 nm (292 and 700...
Article
A monolayer of HeLa cells in plateau-phase of growth was exposed to He-Ne laser radiation either 5 min, 60 min or 180 min before (gamma) -irradiation. It has been shown that in the case of a 5-min interval between the two types of irradiations the survival curve was practically identical to the survival curve of the cells after (gamma) -irradiation...
Article
A monolayer of HeLa cells in plateau-phase of growth was exposed to He-Ne laser radiation either 5 min, 60 min or 180 min before (gamma) -irradiation. It has been shown that in the case of a 5-min interval between the two types of irradiations the survival curve was practically identical to the survival curve of the cells after (gamma) -irradiation...
Article
The structure of mitochondria was studied after irradiation of human lymphocytes with an HeNe laser. Ultrathin sections of the lymphocytes were studied by electron microscopy 1 h after the irradiation. The irradiation resulted in a 20 percent increase in the mean number of mitochondrial profiles on cell section without increase in their total area....
Article
Chemiluminescence test results were used to evaluate the sensitivity of human blood and murine splenocytes to continuous-wave (CW) and pulsed He-Ne laser light. It is demonstrated that CW radiation has in our experimental conditions practically no effect on the luminol-amplified chemiluminescence of four models under study. The pulsed radiation had...
Article
Yeast Torulopsis sphaerica were irradiated with a He-Ne laser (632.8 nm) at 460 or 1150 J/m2 and incubated for 18 h in fresh medium, after which morphometric analysis was conducted on ultrathin sections. Low irradiation decreased the relative number of mitochondrial profiles per section, but increased the mean profile area, relative area of cristae...
Article
Biological responses of cells to visible and near IR (laser) radiation occur due to physical and/or chemical changes in photoacceptor molecules, components of respiratory chains (cyt a/a3 in mitochondria). As a result of the photoexcitation of electronic states, the following physical and/or chemical changes can occur: alteration of redox propertie...
Article
Biological responses of cells to visible and near IR (laser) radiation occur due to physical and/or chemical changes in photoacceptor molecules, components of respiratory chains (cyt a/a3 in mitochondria). As a result of the photoexcitation of electronic states, the following physical and/or chemical changes can occur: alteration of redox propertie...
Article
Luminol-amplified chemiluminescence was recorded after irradiation with laser radiation at 632.8 and 820 nm. The following cellular systems were used as objects of irradiation: blood of healthy donors, blood of patients with colon cancer or acute respiratory illness, blast cells of patients with acute leukemia. The irradiation was suppressing the o...
Article
BACKGROUND and The adhesion of HeLa cells was evaluated after irradiation with monochromatic low-intensity light or laser irradiation. It is well known that the cell-cell and cell-matrix adhesion changes during wound repair. For better understanding of low-power laser light action on the wound healing process, it would be of interest to study the l...
Article
Low-power laser radiation of visible and near IR spectral ranges is widely used in clinical practice to treat wounds and ulcers of various etiologies. However, there are only a few works devoted to photobiological studies of the effect of light of these spectral ranges on the metabolism of nonphotosynthesizing cells. Our results suggested that the...
Article
Biological responses of cells to visible and near IR (laser) radiation occur due to physical and/or chemical changes in photoacceptor molecules, components of respiratory chains (cyt a/a3 in mitochondria, and cyt d in E. coli). As a result of the photoexcitation of electronic states, the following physical and/or chemical changes can occur: alterat...
Article
A monolayer of HeLa cells was irradiated with an He-Ne laser (632.8 nm, 100 J m-2, 10 s) and the amount of adenosine triphosphate (ATP) was measured by the luceferin-luciferase bioluminescent assay technique at different times (5-45 min) after irradiation. The amount of ATP in the log phase of cultured cells remained at the control level (0.79 +/-...
Article
Bacterial suspensions in a phosphate buffer were irradiated at wavelengths lambda of 632.8, 1066 and 1286 nm, incubated in Hottinguer broth for 60 min and assayed for viability by the standard surface-plating technique. The difference between the number of viable cells in the irradiated culture and the control was termed growth stimulation. Irradia...

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