Tiffany Lodge

Tiffany Lodge
University of Oxford | OX · Nuffield Department of Obstetrics and Gynaecology

DPhil (Oxon)

About

19
Publications
2,078
Reads
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201
Citations
Additional affiliations
August 2017 - December 2019
University of Oxford
Position
  • Researcher
October 2009 - September 2011
University of Oxford
Position
  • Biobank technician

Publications

Publications (19)
Article
Full-text available
Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS), a chronic disease characterized by long-lasting persistent debilitating widespread fatigue and post-exertional malaise, remains diagnosed by clinical criteria. Our group and others have identified differentially expressed miRNA profiles in the blood of patients. However, their diagnostic...
Article
Full-text available
Age-related neuronal dysfunction can be overcome by circulating factors present in young blood. Growth differentiation factor-11 (GDF-11), a systemic factor that declines with age, can reverse age-related dysfunction in brain, heart and skeletal muscle. Given that age increases susceptibility to stroke, we hypothesized that GDF-11 may be directly p...
Article
Full-text available
Background: Autosomal dominant optic atrophy (ADOA) is usually caused by mutations in the essential gene, OPA1. This encodes a ubiquitous protein involved in mitochondrial dynamics, hence tissue specificity is not understood. Dysregulated mitophagy (mitochondria recycling) is implicated in ADOA, being increased in OPA1 patient fibroblasts. Furtherm...
Data
Mapping of the mRFP transgene. The insertion of the mRFP transgene was investigated by paired-end sequencing. The insert is located in the 3rd exon of the pkn1 gene, which has been confirmed by PCR using a common forward primer (green) and two different reverse primers; one (red) 1,500 bp from the forward primer in the wild type genome (blue) and o...
Data
Analysis of OPA1 expression in the MEFs used. Protein extracts were prepared from wt, RedMIT-GFP-LC3 and RedMIT-GFP-LC3-OPA1Q285STOP MEFs and analyzed on a 8% acrylamide gel. The OPA1 signal was detected using a rabbit anti-OPA1 antibody (abcam ab42364) and revealed using a polyclonal goat anti-rabbit secondary antibody (Dako P0448) coupled to an E...
Data
mRFP and GFP transgenes do not affect mice reproductive success.
Data
Images from sections of brain and spleen from control, RedMIT/GFP-LC3, and OPA1Q285STOP/RedMIT/GFP-LC3. Brain (Bottom) and spleen (Top) from non-fluorescent (control), RedMIT/GFP-LC3 and OPA1Q285STOP/RedMIT/GFP-LC3 mice were sectioned (10 μm) and imaged on a Zeiss LSM 700 inverted confocal microscope with a plan-Apo 63x NA 1.4 oil-immersion objecti...
Data
Movie of the RedMIT-GFP-LC3 MEFs. Live RedMIT-GFP-LC3 MEFs have been imaged using a custom Olympus IX81 inverted microscope equipped with temperature control (Solent scientific) every 30 s for 8 h. This can be used to quantify the early stages of mitophagy in real time.
Article
Full-text available
Background : Mitochondrial diabetes is primarily caused by β-cell failure, a cell type whose unique properties are important in pathogenesis. Methods : By reducing glucose, we induced energetic stress in two rodent β-cell models to assess effects on cellular function. Results : Culturing rat insulin-secreting INS-1 cells in low glucose conditions c...
Article
Full-text available
Background: Mitochondrial diabetes is primarily caused by β-cell failure, but there are gaps in our understanding of pathogenesis. Methods: By reducing glucose, we induced energetic stress in two rodent β-cell models to assess effects on cellular function. Results: Culturing rat insulin-secreting INS-1 cells in low glucose conditions caused a rapid...
Article
Full-text available
Herein we have undertaken a systematic analysis of the effects of the fungal derivative ophiobolin A (OphA) on eight cancer cell lines from different tissue types. The LD50 for each cell line was determined and the change in cell size determined. Flow cytometric analysis and western blotting were used to assess the cell death markers for early apop...
Article
Full-text available
One in 400 people has a maternally inherited mutation in mtDNA potentially causing incurable disease. In so-called heteroplasmic disease, mutant and normal mtDNA co-exist in the cells of carrier women. Disease severity depends on the proportion of inherited abnormal mtDNA molecules. Families who have had a child die of severe, maternally inherited...
Article
Full-text available
Maternally inherited mitochondrial DNA (mtDNA) mutations cause symptoms of Leber hereditary optic neuropathy (LHON) in -1 in 30,000 individuals. Most of the affected individuals lack respiratory chain defects1 and there is no proven prophylactic treatment.
Article
Mitophagy is a cellular mechanism for the recycling of mitochondrial fragments. This process is able to improve mitochondrial DNA (mtDNA) quality in heteroplasmic mtDNA disease, in which mutant mtDNA co-exists with normal mtDNA. In disorders where the load of mutant mtDNA determines disease severity it is likely to be an important determinant of di...

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Projects

Projects (2)
Project
Registration is now open for the annual MItOX meeting. please follow link https://www.obs-gyn.ox.ac.uk/news/mitox-2017-bookings-now-open
Project
Investigate cellular perturbations in ME/Chronic Fatigue Syndrome