Tiago Pereira

Tiago Pereira
University of Porto | UP · Instituto de Ciências Biomédicas Abel Salazar (ICBAS)

DVM GPCert(EqP) PhD

About

62
Publications
9,071
Reads
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768
Citations
Citations since 2016
36 Research Items
641 Citations
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2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
Introduction
Additional affiliations
May 2009 - September 2015
University of Porto
Position
  • Professor (Assistant)

Publications

Publications (62)
Article
Full-text available
Mesenchymal Stem/ Stromal Cells assume a supporting role to the intrinsic mechanisms of tissue regeneration, a feature mostly assigned to the contents of their secretome. A comparative study on the metabolomic and bioactive molecules/factors content of the secretome of Mesenchymal Stem/ Stromal Cells derived from two expanding sources: the umbilica...
Preprint
Full-text available
Mesenchymal Stem/ Stromal Cells assume a supporting role to the intrinsic mechanisms of tissue regeneration, a feature mostly assigned to the contents of their secretome. A comparative study on the metabolomic and bioactive molecules/factors content of the secretome of Mesenchymal Stem/ Stromal Cells derived from two expanding sources: the umbilica...
Article
Full-text available
Development of synthetic bone substitutes has arisen as a major research interest in the need to find an alternative to autologous bone grafts. Using an ovine model, the present pre-clinical study presents a synthetic bone graft (Bonelike®) in combination with a cellular system as an alternative for the regeneration of non-critical defects. The ass...
Article
Full-text available
Mesenchymal Stromal cells (MSCs) have a potential role in cell-based therapies. Foetal bovine serum (FBS) is used to supplement the basal cell culture medium but presents several disadvantages and risks. Other alternatives have been studied, including human umbilical cord blood plasma (hUCBP), aiming at the development of xeno-free culturing protoc...
Data
UCB samples analysis. Initial WBC count, performed before the volume reduction with the AXP automated system (cells x 109 per liter) and the final WBC count (cells x 109 per liter), performed after the volume reduction procedure of the UCB samples, values obtained by using the hematology auto-analyser (Ac T diff2™, Beckman Coulter, Inc.). Total num...
Data
Corrected absorbance assessed by PrestoBlue viability assay of hMSCs (UC-MSCs and DPSCs), in the presence of supplemented medium with FBS_II or variable concentrations of hUCBP for up to 9 days. Results presented as Mean ± SEM. (DOCX)
Data
Chondrogenic differentiation. Sulfated GAGs production (μg/ml) after 14 days, assessed by Blyscan Glycosaminoglycan Assay (Biocolor, UK). Control: Undifferentiated control; Chondro Diff: Chondrogenic Differentiation. Results Presented as Mean ± SEM. (DOCX)
Data
Statistical significance in Oil Red O (OD570nm) after 14 days. C: Undifferentiated control; D: Adipogenic Differentiation. Significance of the results is indicated according to P values with one, two, three or four of the symbols (*) corresponding to 0.01≤P<0.05; 0.001≤P< 0.01; 0.0001≤P<0.001 and P<0.0001, respectively; ns, not significant. (DOCX)
Data
Statistical significance differences in sulfated GAGs production (μg/ml) after 14 days, assessed by Blyscan Glycosaminoglycan Assay (Biocolor, UK). C: Undifferentiated control; D: Chondrogenic Differentiation. Significance of the results is indicated according to P values with one, two, three or four of the symbols (*) corresponding to 0.01≤P<0.05;...
Data
Total RNA extracted from UC-MSCs and DPSCs cultured in hUCBP or FBS supplemented media, readings at 260 and 280 nm. (DOCX)
Data
β-Galactosidase activity assay (OD405nm) on UC-MSCs and DPSCs at 3, 5 and 7 days. Results Presented as Mean ± SEM. (DOCX)
Data
Annexin V/ PI detection on UC-MSCs and DPSCs after 5 days of culture in hUCBP or FBS supplemented media. Results Presented as Mean ± SEM. (DOCX)
Data
Quantitative PCR of UC-MSCs and DPSCs cultured in hUCBP or FBS supplemented media. Avg ΔCq: average quantification cycle (differential expression of target and housekeeping genes); ΔΔCq: differential expression of sample (4%, 6% and 8% hUCBP) and reference sample (FBS 10%) genes; RQ: relative quantification (fold change compared to the FBS 10% grou...
Data
Osteogenic differentiation. Alizarin Red S concentration (μM) after 21 days. Control: Undifferentiated control; Osteo Diff: Osteogenic Differentiation. Results Presented as Mean ± SEM. (DOCX)
Data
Statistical significance in Alizarin Red S concentration (μM) after 21 days. C: Undifferentiated control; D: Osteogenic Differentiation. Significance of the results is indicated according to P values with one, two, three or four of the symbols (*) corresponding to 0.01≤P<0.05; 0.001≤P< 0.01; 0.0001≤P<0.001 and P<0.0001, respectively; ns, not signif...
Data
Adipogenic differentiation. Oil Red O (OD570nm) after 14 days. Control: Undifferentiated control; Adipo Diff: Adipogenic Differentiation. Results Presented as Mean ± SEM. (DOCX)
Article
Peripheral nerve regeneration following severe events is still a challenging topic in the regenerative medicine field, especially when nerve tissue is lost and direct suturing is not feasible. Given the limited success observed in currently available techniques, researchers have been putting efforts towards the development and optimization of these...
Article
The functional and structural performance of a 5 cm synthetic small diameter vascular graft (SDVG) produced by the copolymerization of polyvinyl alcohol hydrogel with low molecular weight dextran (PVA/Dx graft) associated to mesenchymal stem cells (MSCs)-based therapies and anticoagulant treatment with heparin, clopidogrel and warfarin was tested u...
Article
The therapeutic effect of three polyvinyl alcohol (PVA) membranes loaded with electrically conductive materials - carbon nanotubes (PVA-CNTs) and polypyrrole (PVA-PPy) - were tested in vivo for neuro-muscular regeneration after an axonotmesis injury in the rat sciatic nerve. The membranes electrical conductivity measured was 1.5±0.5x10(-6) S/m, 579...
Chapter
Full-text available
Mesenchymal stem cells (MSCs) are multipotent stromal cells with a strong potential in human regenerative medicine due to their ability to renew themselves and differentiate into various specialized cell types under certain physiological or experimental conditions. MSCs secrete a broad spectrum of autocrine and paracrine factors (MSCs' secretome) t...
Article
Full-text available
Increasing relevance has been attributed to hydrogels due to their ability to provide an extracellular matrix (ECM)-like environment for cellular adhesion and proliferation, acting as mechanical scaffolds for tissue remodeling or as delivery matrices. In vivo biocompatibility of a hybrid dextrin hydrogel produced from oxidized dextrin and adipic ac...
Article
The functional and structural performance of a 5 cm synthetic small diameter vascular graft (SDVG) produced by the copolymerization of polyvinyl alcohol hydrogel with low molecular weight dextran (PVA/Dx graft) associated to mesenchymal stem cells (MSCs)-based therapies and anticoagulant treatment with heparin, clopidogrel and warfarin was tested u...
Article
Peripheral nerve regeneration is a serious clinical problem. Presently, there are several nerve tube-guides available in the market, however with some limitations. The goal of this work was the development of a biomaterial with high electrical conductivity to produce tube-guides for nerve regeneration after neurotmesis injuries whenrver an end-to-e...
Article
Full-text available
Mesenchymal stem cells are posing as a promising character in the most recent therapeutic strategies and, since their discovery, extensive knowledge on their features and functions has been gained. In recent years, innovative sources have been disclosed in alternative to the bone marrow, conveying their associated ethical concerns and ease of harve...
Chapter
Full-text available
Skeletal muscle injuries are quite frequent in traumatic scenarios, such as war injuries or road-or work-related accidents. The skeletal muscle has good regenerative ability, but the extent or recurrence of muscle injury might impair complete structural and functional recovery. Severe tissue loss overwhelms skeletal muscle´s intrinsic regenerative...
Article
Full-text available
To study the therapeutic effect of three tube-guides with electrical conductivity associated to mesenchymal stem cells (MSCs) on neuro-muscular regeneration after neurotmesis. Rats with 10-mm gap nerve injury were tested using polyvinyl alcohol (PVA), PVA-carbon nanotubes (CNTs) and MSCs, and PVA-polypyrrole (PPy). The regenerated nerves and tibial...
Article
Full-text available
Peripheral nerves possess the capacity of self-regeneration after traumatic injury but the extent of regeneration is often poor and may benefit from exogenous factors that enhance growth. Neonatal tissues are routinely discarded at parturition so little ethical controversy attends the harvest of the Mesenchymal Stem Cells (MSCs) which may play an i...
Article
Full-text available
Skeletal muscle is essential in voluntary movement and other major vital functions. Muscle injuries are important in clinical practice and, despite skeletal muscle's good regenerative ability, severe tissue loss impairs complete myofibre regeneration, limiting structural and functional recovery of the affected muscle, eventually leading to the deve...
Article
Full-text available
Human mesenchymal stem cells (hMSCs) from umbilical cord (UC) blood (UCB) and matrix are tested clinically for a variety of pathologies but in vitro expansion using culture media containing fetal bovine serum (FBS) is essential to achieve appropriate cell numbers for clinical use. Human UCB plasma (hUCBP) can be used as a supplement for hMSCs cultu...
Article
Full-text available
Skeletal muscle has good regenerative capacity, but the extent of muscle injury and the developed fibrosis might prevent complete regeneration. The in vivo application of human mesenchymal stem cells (HMSCs) of the umbilical cord and the conditioned media (CM) where the HMSCs were cultured and expanded, associated with different vehicles to induce...
Article
Full-text available
The healing process of the skin is a dynamic procedure mediated through a complex feedback of growth factors secreted by a variety of cells types. Despite the most recent advances in wound healing management and surgical procedures, these techniques still fail up to 50%, so cellular therapies involving mesenchymal stem cells (MSCs) are nowadays a p...
Article
Full-text available
In peripheral nerves MSCs can modulate Wallerian degeneration and the overall regenerative response by acting through paracrine mechanisms directly on regenerating axons or upon the nerve-supporting Schwann cells. In the present study, the effect of human MSCs from Wharton's jelly (HMSCs), differentiated into neuroglial-like cells associated to pol...
Data
Table 3 represents values in seconds (s) that were obtained performing Withdrawal Reflex Latency (WRL) test to evaluate the nociceptive function. This test has been performed pre-operatively (week-0), at week 1 and 2 and after every two weeks until the end of the 20-week follow-up time. Results are presented as mean and standard deviation (SD). N c...
Article
Full-text available
Peripheral nerves have the intrinsic capacity of self-regeneration after traumatic injury but the extent of the regeneration is often very poor. Increasing evidence demonstrates that mesenchymal stem/stromal cells (MSCs) may play an important role in tissue regeneration through the secretion of soluble trophic factors that enhance and assist in rep...
Article
Full-text available
Presently, several bone graft substitutes are being developed or already available for clinical use. However, the limited number of clinical and in vivo trials for direct comparison between these products may complicate this choice. One of the main reasons for this scarcity it is the use of models that do not readily allow the direct comparison of...
Article
Full-text available
Polyvinyl alcohol hydrogel (PVA) is a synthetic polymer with an increasing application in the biomedical field that can potentially be used for vascular grafting. However, the tissue and blood-material interactions of such gels and membranes are unknown in detail. The objectives of this study were to: (a) assess the biocompatibility and (b) hemocom...
Article
Human mesenchymal stem cells (hMSCs) from umbilical cord (UC) blood (UCB) and matrix are tested clinically for a variety of pathologies but in vitro expansion using culture media containing fetal bovine serum (FBS) is essential to achieve appropriate cell numbers for clinical use. Human UCB plasma (hUCBP) can be used as a supplement for hMSCs cultu...
Article
Mesenchymal stem cells (MSCs) from Wharton's jelly present high plasticity and low immunogenicity, turning them into a desirable form of cell therapy for the injured nervous system. Their isolation, expansion, and characterization have been performed from cryopreserved umbilical cord tissue. Great concern has been dedicated to the collection, prese...
Chapter
The isolation and culture of MSCs from the Wharton’s jelly was performed by our research group in order to obtain undifferentiated MSCs and in vitro differentiated into neural-like cells to be tested in axonotmesis and neurotmesis lesions of the rat sciatic nerve. The isolation has been performed by enzyme-free tissue explant and enzymatic isolatio...
Chapter
Long-standing histological characteristics are still used to identify the mammalian skeletal muscle regeneration process. On muscle cross-sections, these fundamental morphological characteristics are newly formed myofibers of small caliber and with centrally located myonuclei. Newly formed myofibers are often basophilic (reflecting high protein syn...
Article
Corrigendum to "Use of poly(DL-lactide-ε-caprolactone) membranes and mesenchymal stem cells from the Wharton's jelly of the umbilical cord for promoting nerve regeneration in axonotmesis: In vitro and in vivo analysis" [Differentiation 84 (2012) 355–365] The authors regret that they omitted four authors from the original article; the correct author...
Article
Cellular systems implanted into an injured nerve may produce growth factors or extracellular matrix molecules, modulate the inflammatory process and eventually improve nerve regeneration. In the present study, we evaluated the therapeutic value of human umbilical cord matrix MSCs (HMSCs) on rat sciatic nerve after axonotmesis injury associated to V...
Article
Full-text available
Many studies have been dedicated to the development of scaffolds for improving post-traumatic nerve regeneration. The goal of this study was to assess the effect on nerve regeneration, associating a hybrid chitosan membrane with non-differentiated human mesenchymal stem cells isolated from Wharton's jelly of umbilical cord, in peripheral nerve reco...

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