Tiago Lucini

None · Entomology

Feeding behaviors of the redbanded stink bug, Piezodorus guildinii (Westwood), on vegetative (stem and leaflet) and reproductive (pod) tissues of soybean, Glycine max (L.), were recorded using an AC-DC electropenetrograph (EPG) apparatus. Eight different probing waveforms were characterized and defined: Pg1a, Pg1b, Pg1c, Pg1d, Pg2, Pg3a, Pg3b, and...

The green belly stink bug, Dichelops melacanthus (Dallas), has become an important pest of maize in the Neotropics in recent years. Thus, more studies are needed to understand its feeding behavior in order to find ways to reduce its damage. One way to do so is through the technique known as electrical penetration graph (EPG), which allows unveiling...

Once abundant in the Americas, the southern green stink bug Nezara viridula (L.) has gradually declined in numbers. Until the 1990s, it was considered the main pest of major crops such as soybean, Glycine max (L.) Merrill, particularly in southern Brazil and southern USA, as well as Argentina, Uruguay, and other countries. In the past 15+ years, a...

The stink bug Dichelops furcatus (F.) (Heteroptera: Pentatomidae) has increased in abundance in recent years on the wheat, Triticum aestivum L., crop cultivated in the southern region of Brazil. To investigate the probing (stylet penetration) behaviors and nonprobing behaviors of D. furcatus on wheat plants, the electrical penetration graph or elec...

Host plant resistance has been known for a long time as one of the most important strategies to manage insect pest species. In the case of stink bugs that impact the soybean crop, attempts were done to develop resistant cultivars in the past with limited favorable results. These limitations were, in general, due to the relatively low yield of grain...

Life history studies are fundamental in order to the design of holistic integrated pest management programs (IPM). It impacts on the planning of the crop, for instance, in the choice of the cultivar, time of sowing, weed management, crop rotation among other issues. The Neotropical Region is one of the greater producers of agricultural commodities...

The study aimed to understand the feeding behavior of Neotropical brown stink bug nymphs Euschistus heros (F.) on soybean plants during vegetative stage through electropenetrography (EPG) technique. Three distinct phases were identified: non-feeding, pathway, and ingestion. Waveforms representing these phases were consistent across nymphal instars...

In this article, we present and discuss the importance of life history information of four species of stink bugs (Heteroptera: Pentatomidae) of the neotropics, pests of major commodities, as basic information necessary for development of improved programs of conservation biological control. This information is from cultivated areas in two ranges in...

Bt soybean cultivation is increasing worldwide. The Cry1Ac protein expressed in Bt soybean efficiently controls several lepidopteran pests. The stink bug, Piezodorus guildinii (Westwood), a major pest for soybean in the Americas, is not controlled by Bt crops, although possible sub-lethal effects may occur. Even if there were no negative effects fo...

This study aimed to characterize and correlate the stylet penetration behaviors of nymphs of the Neotropical brown stink bug, Euschistus heros (F.), on immature soybean pods. Waveforms were obtained using electropenetrography (EPG). The findings revealed that the nymphs exploited the xylem vessels and the seed tegument or endosperm. Primarily 4 pha...

Studies were conducted in the laboratory and in the greenhouse with the objective of evaluating nymph development, adult body weight gain, and damage of different species of pentatomids feeding on fruit (hereafter referred to as siliqua) of canola, Brassica napus (L.) var. oleifera. Nymphs of Nezara viridula (L.) were able to reach adulthood feedin...

Laboratory and greenhouse studies were conducted with the Neotropical brown stink bug, Euschistus heros (F.), to evaluate and compare adult damage at two infestation levels (2 and 4 adults/plant) and feeding behavior on seeds of three resistant soybean cultivars bearing the Block technology ('BRS 1003 IPRO', 'BRS 543 RR', and 'BRS 391') compared to...

Most stink bugs (Pentatomidae) are polyphagous and feed on an array of cultivated and non-cultivated plants. Among the last, weed plants play an important role in their bioecology and pest status, particularly in the Neotropics, in where stink bugs are active during most of the year. In spite of this, the weeds role is, in general, underestimated....

In this review article, we present and discuss the main factors influencing the change in pest status of phytophagous stink bugs (Hemiptera: Heteroptera: Pentatomidae) in the Neotropics. We have surveyed the published records over the past 50 years and divided this timeframe into decades. This was done to rank in time the relative abundance (percen...

This book compiles for the first time all the current information on the electronic monitoring of the feeding behaviour of phytophagous true bugs, describing the mouthparts and modes of feeding of. It includes state-of-the-art illustrations of feeding sites on the various plant structures, and examines how the different feeding strategies are relat...

In this concluding chapter, we discuss the value of EPG in electronic monitoring of all activities of phytophagous true bugs. Although the majority of studies have concentrated on feeding behavior, EPG is a tool with the potential to reveal details of any activity that generates an electrical connection between the bug and the plant, which expands...

Since its development, the electrical penetration graph (EPG) has been used to record the feeding behavior of mostly small, relatively weak insects (aphids and leafhoppers). Recently the larger, more robust true bugs (e.g., pentatomids) began to be monitored via EPG, and some problems were encountered, especially related to gold wire attachment. Wi...

The multiple interactions of the electrical penetration graph (EPG) technique with various strategies that can be used to manage pests of crop plants are presented and discussed in this chapter. We here include insecticides, still the main tool in use to control true bug pests worldwide by either contact action or systemic circulation in plants; pl...

Electrical penetration graph technique, or electropenetrography (EPG), is a method of indirectly visualizing the feeding of piercing-sucking insects within plant tissue, permitting quantification of feeding behaviors such as stylet penetration, salivation, and ingestion from specific plant tissues. In this chapter, the basic principles of EPG are e...

Phytophagous true bugs feed on virtually all plant parts, both vegetative and reproductive, from roots to shoots and buds to seeds. Preferred ingestion sites include vascular tissue; parenchyma of stems, leaves, and fruits; and seed endosperm. Stylet length and depth of penetration, production of a salivary sheath from gelling saliva, and enzymatic...

Heteropteran mouthparts consist of elongate mandibular and maxillary stylets encased at rest ventrally within the labial sheath. The greatly reduced labrum covers the stylets proximally as they emerge from the head. The two mandibular stylets surround the two maxillae, which are appressed. The food and salivary canals are formed by grooves and inte...

In this chapter, we summarize and discuss the most relevant feeding activities and their respective EPG waveforms performed by true bugs from four families, Miridae (infraorder Cimicomorpha), Blissidae, Coreidae, and Plataspidae (infraorder Pentatomomorpha). Recordings performed with species of Blissidae, Coreidae, and Plataspidae share similar wav...

Stink bugs (Pentatomidae) encompass a great variety of heteropterans, many of them important pest species. Electronic monitoring of feeding behavior of pentatomids using the electrical penetration graph (EPG) technique has been conducted over a relatively short time span (10 years). To date, eight pentatomid species have been studied with EPG: Dice...

In this study, we use for the first time the electropenetrography (EPG) technique to characterize and describe the feeding activities of Collaria scenica (Stål) adults and their respective waveforms on wheat, Triticum aestivum L. plants. It comprised of four waveforms, two related to non-feeding (Np and R) and two with feeding activities (CR and I)...

Report on the true bugs (Pentatomidae, Miridae) pests of wheat in Brazil, with data on their biology, association with other species of Poaceae, and their damage to wheat (in Portuguese). Version online and printed.

Laboratory studies were conducted with the Neotropical brown stink bug, Euschistus heros (F.), to evaluate nymphal and adult biology on immature pods of soybean, Glycine max (L.) Merrill (Fabaceae), bearing the block technology (resistant to stink bug damage – cvs. BRS 391, BRS 543 RR and BRS 1003 IPRO) compared to a susceptible cultivar (BRS 5601...

A survey of stink bugs (Hemiptera: Heteroptera: Pentatomidae) was conducted on the invasive tree, Pittosporum undulatum Ventenat (Pittosporaceae) in southern Brazil (Passo Fundo – RS, latitude 28°15’46” S; longitude 52°24´24” W) aiming to know if this exotic plant might host these insects. Results indicated that P. undulatum was found hosting the s...

We used electropenetrography to quantify and compare counts and durations of selected waveforms, produced by adult females of the stink bug Dichelops furcatus (F.). Insects fed on immature soybean pods and immature seed heads of four spring cereals: wheat, black oat, barley, and rye. On all foods, bugs spent over 60% of their plant access time in n...

Laboratory studies with the stink bug Chinavia erythrocnemis (Berg) (Hemiptera: Pentatomidae) were conducted to evaluate the effect of reproductive structures of cultivated plants, on nymph and adult performance, and adult preference. Results indicated that nymphs were able to complete the development on all plants evaluated. Nymph developmental ti...

Field and laboratory studies were conducted with the Neotropical red-shouldered stink bug Thyanta perditor (F.) (Heteroptera: Pentatomidae) aiming to evaluate parasitism incidence on adults by tachinid flies (Diptera: Tachinidae), which were raised in the laboratory for identification. Egg deposition by flies on adult body surface was mapped. In ad...

Laboratory studies were conducted with Dichelops furcatus (F.), Euschistus heros (F.), and Nezara viridula (L.) (Heteroptera: Pentatomidae) aiming to evaluate nymph and adult biology and adult preference for immature reproductive structures of the cultivated plants, soybean, Glycine max (L.) Merrill (Fabaceae), wheat, Triticum aestivum L. (Poaceae)...

Damage inflicted by the gree-belly stink bug, Dichelops furcatus (F.) to winter (spring) cereals and canola seedlings is illustrated and described. The destruction of the leaf tissues are followed daily for about a week time after the feeding activity of an adult bug, and fully characterized using a sequence of photographs.

The stink bug Dichelops furcatus (F.) (Heteroptera: Pentatomidae) is commonly found on soybean plants (Glycine max (L.) Merrill) in Brazil, and recently it has increased in abundance on spring cereals, mostly on wheat crop, Triticum aestivum L., in the southern region of Brazil. To compare the feeding behavior of this stink bug species on different...

This poster demonstrates that the red-banded stink bug, Piezodorus guildinii does not have its feeding behavior affected when feeding on RR/Bt soybean compared to non-Bt cultivars.

In this workshop the latest news on stink bug research work using EPG will be presented and discussed.

The Neotropical green-belly stink bug, Dichelops melacanthus (Dallas) is a major pest of maize, Zea mays L. in the main production areas of Brazil. It usually feeds on the stems of young plants (seedlings) causing heavy damage by affecting the plant growth and mitigating seed yield. Laboratory studies were conducted to determine body position (upwa...

Laboratory studies demonstrated that nymphs of Thyanta perditor (F.) completed their development on seed heads of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.), and on immature and mature seeds of black jack (Bidens pilosa L.), whereas no nymphs passed to the third instar when fed with leaves of any plant evaluated. At adult emergenc...

Laboratory and field studies were conducted with nymphs and adults of the Neotropical stink bug Runibia perspicua (F.) to evaluate their biology on plants. Total nymph mortality was ca. 13% on immature fruits of Brunfelsia australis Benth. (IFBA), and 90% on raw shelled peanuts, Arachis hypogaea L. (RSP); no nymphs survived on immature pods of gree...

The Neotropical brown-stink bug Euschistus heros (F.) (Hemiptera: Pentatomidae) is the most important pest damaging soybean in the Neotropics, the world largest production area. The alternating current–direct current (AC–DC) electropenetrography (EPG) technology was used to develop an EPG waveform library of adult females feeding on soybean (Glycin...

Estudos com o percevejo Thyanta perditor (F.) (Hemiptera: Heteroptera) foram conduzidos em laboratório para avaliar o efeito de vagens e espigas das plantas cultivadas soja (Glycine max (L.) Merrill), trigo (Triticum aestivum L.), cevada (Hordeum vulgare L.), triticale (Triticosecale semisecale (Mackey)), centeio (Secale cereale L.) e aveia (Avena...

O percevejo marrom, Euschistus heros (F.) (Hemiptera: Heteroptera), é a principal praga da cultura soja, Glycine max (L.) Merrill, no Brasil. Para compreender melhor suas atividades alimentares em vagens de soja, a técnica da electropenetrografia (EPG) foi utilizada para monitorar e caracterizar as formas de onda produzidas por fêmeas adultas em va...

The performance and preferences of the stink bug, Dichelops furcatus (F.), for spring cereals (wheat, rye, triticale, oat, and barley) were compared in the laboratory to their preferred host crop, soybean pods. Nymphs took significantly less time to reach adulthood on soybean pods compared to those fed seed heads of the five spring cereals tested....

Tomato genotypes selected for their high foliar zingiberene (ZGB) contents in a segregating F2 population were assessed to determine their effect on behavior and biology of Tetranychus urticae Koch, the putative resistance mechanisms involved and the role of trichomes on that resistance. Genotypes with contrasting ZGB content (RVTZ-09 = low ZGB, RV...

Monthly parasitism rates and oviposition behavior of tachinid flies parasitizing Dichelops furcatus (F.) (Hemiptera: Pentatomidae) adults were determined. Weekly surveys, with duration of 2 hr each, were performed during 1 yr at the National Wheat Research Center in Passo Fundo, Rio Grande do Sul, Brazil. Stink bugs were collected from different pl...

In this article, we review and discuss the potential use of EPG (electropenetrography) as a powerful tool to unveil the feeding process of phytophagous stink bugs (pentatomids). These bugs are relatively big and vigorous, which presents a problem during wiring (i.e., attachment of the gold wire on the bug’s pronotum) for use in EPG. Once this chall...

The stink bug Dichelops furcatus (F.) (Heteroptera: Pentatomidae) has increased in abundance in recent years on wheat, Triticum aestivum L., crop cultivated in the southern region of Brazil. Herein, we used the technology known as electropenetrography (EPG), to assess its feeding behavior on wheat in different phenological stages (vegetative and re...

Abstract of poster included.

Laboratory and field studies were conducted with nymphs and adults of the Neotropical stink bug Runibia perspicua (F.) to evaluate their biology on plants. Total nymph mortality was ca. 13% on immature fruits of Brunfelsia australis Benth. (IFBA), and 90% on raw shelled peanuts, Arachis hypogaea L. (RSP); no nymphs survived on immature pods of gree...

Laboratory studies were conducted on food ingestion and excretion by selected species of pentatomids on different food sources to support their pest status. We compared the frequency and time of feeding on vegetative (stem) and reproductive (seed) structures of soybean, Glycine max (L.) Merrill and of maize, Zea mays L. by Piezodorus guildinii (Wes...

Laboratory studies with Euschistus cornutus Dallas indicated that nymphs complete development when feeding on green bean, Phaseolus vulgaris L. pod, on soybean, Glycine max (L.) Merrill pod, and on raw shelled peanut, Arachis hypogaea L., but not on fruit (berry) of privet, Ligustrum lucidum Ait. Total mortality was lower on green bean pod (45%), a...

The uncommon Cyrtocoris egeris Packauskas & Schaefer (Heteroptera: Pentatomidae) was collected in 2015 and 2016 in Francisco Beltrão Co. (26.07°S, 53.05°W), Paraná State, and in Mato Castelhano Co. (28.27°S, 52.18°W), Rio Grande do Sul State, Brazil. They fed and reproduced on plants of the Brazilian orchid tree, Bauhinia forficata Link (Fabaceae)...

Greater activity and movement caused the wire to break more often with heavier/larger bugs. Cuticular lipid removal by sanding improved gold wire attachment. EPG of E. meditabunda on soybean stems generated six probing waveforms grouped into four phases: pathway, X wave, ingestion, and interruption phases. Recordings of P. guildinii revealed eight...

Several studies have been conducted on resistance sources to improve the genetic resistance of farm-grown tomatoes to arthropod pests, including phytophagous mites. In the present study, we evaluate the behavior of the two-spotted spider mite on different cherry tomato accessions to identify possible sources of resistance (repellent effect) to this...

The poorly known stink bug, Edessa loxdalii Westwood (Hemiptera: Heteroptera: Pentatomidae) was intercepted in Francisco Beltrão, Paraná State, Brazil (26.0808333°S, 53.0547222°W) feeding and reproducing on soybean, Glycine max (L.) Merrill (Fabales: Fabaceae), on oriental raisin tree, Hovenia dulcis Thunb. (Rosales: Rhamnaceae), and on the wild so...

Stink bugs (Hemiptera: Heteroptera: Pentatomidae) are in general robust and restless insects, which makes them difficult to wire for electropenetrograph (EPG) studies. In addition, cuticular lipids may reduce wire effectiveness, and their removal could improve success of wiring. We compared wiring effectiveness for three species of stink bugs, diff...

The green belly stink bug, Dichelops furcatus (F.) (Hemiptera: Heteroptera: Pentatomidae) is a pest of corn and soybean in southern Brazil. It also occurs on wheat, but information on its damage to this crop is limited. To determine the need for sustainable IPM programs, the impact of this bug on wheat production should be determined. Studies were...

Stink bugs (Pentatomidae) are in general robust and restless insects, which makes them difficult to wire for electropenetrography (EPG) studies. In addition, cuticular lipids may reduce wire effectiveness and their removal could improve success of wiring. We compared wiring effectiveness for three species of stink bugs, each with different movement...

Feeding behaviors of the redbanded stink bug, Piezodorus guildinii (Westwood) on vegetative (stem and leaflet) and reproductive (pod) tissues of soybean, Glycine max (L.) were recorded using an AC-DC electropenetrograph (EPG) apparatus. Eight different probing waveforms were characterized and defined: Pg1a, Pg1b, Pg1c, Pg1d, Pg2, Pg3a, Pg3b, and Pg...

We evaluated the effect of tomato genotypes with a high content of acylsugar (AS) in the F2 generation on the behavior and biology of Tetranychus urticae, the two-spotted spider mite, the associated resistance mechanisms, and the role of trichomes in this resistance. Genotypes with a high AS content, and resistance to the spider mite (RVTA-2010pl#3...

A preocupação da sociedade com os riscos gerados pelos agrotóxicos, sobre a saúde e o ambiente tem gerado o interesse por formas alternativas de controle, como o uso de extratos vegetais para o controle de artrópodes. O objetivo deste trabalho foi testar o efeito tóxico do extrato aquoso de pimenta-dedo-de-moça Capsicum baccatum (Magnoliopsida: Sol...