Thornthan Sawangwan

• Dr, techn.
• Lecturer at Ramkhamhaeng University

This research investigated and compared the prebiotic properties of a rice bran extract obtained through commercial xylanase extraction in comparison with water extraction. Prebiotic properties were evaluated by probiotic growth stimulation (L. casei and L. plantarum) and gastrointestinal pathogen inhibition (B. cereus and E. coli). The rice bran e...

Currently, the study of mannooligosaccharides (MOS) production from Thai spent coffee grounds (SCGs) and the evaluation of their prebiotic potential have not been documented. This research aimed to characterize the MOS derived from SCGs and to determine their prebiotic properties. SCGs were treated with an alkali treatment. Treated-SCGs were digest...

The prebiotic properties were investigated of seven edible mushrooms: Auricularia auricula-judae, Lentinus edodoes, Pleurotus citrinopileatus, Pleurotus djamor, Pleurotus ostreatus, Pleurotus ostreatus (Jacq.Fr.) Kummer and Pleurotus pulmonarius. All mushrooms were extracted using distilled water and ethanol at a ratio of 1:4 vol per volume, respec...

Spent coffee grounds (SCGs) are solid wastes from the manufacturing of fresh coffee beverages which are globally popular. SCGs are composed of lignocellulosic materials which can be digested by lignocellulolytic enzymes and oligosaccharide is a product from the hydrolysis of cellulose and hemicellulose. However, the presence of lignin in lignocellu...

2-O-(α-D-Glucopyranosyl)-sn-glycerol (αGG) is a natural osmolyte. αGG is produced industrially for application as an active cosmetic ingredient. The biocatalytic process involves a selective transglucosylation from sucrose to glycerol catalyzed by sucrose phosphorylase (SPase). Here we examined immobilization of SPase (from Leuconostoc mesenteroide...

Prebiotic synthesis from economical substrates (glutinous rice and rice bran) using Aspergillus oryzae TISTR 3102 and TISTR 3222 with solid state fermentation (SSF) was explored. The pH value, alpha-glucosidase activity and total reducing sugar (TRS) concentration were monitored during incubation with different substrate ratios of 1:1 and 1:2 weigh...

Isomalto-oligosaccharide from glutinous rice syrups under solid-state fermentation of Aspergillus oryzae TISTR 3102 were evaporated (10-70% w/v of moisture content) and stored in difference temperature (4°C and room temperature) for 12 weeks. The total number of contaminant microorganisms (aerobic, anaerobic, Coliforms, Escherichia coli, mold and y...

Background: Glucosylglycerol (GG), an organic compound constitutes a structurally diverse group of small organic molecules, which has a glycosidic chemical structure, where is a powerful osmolyte which catalyzed by sucrose phosphorylase in the presence of sucrose and glycerol as the donor and acceptor substrate. Context and purpose of this study:...

1,2-Propanediol and 3-aryloxy/alkyloxy derivatives thereof are bulk commodities produced directly from glycerol. Glycosylation is a promising route for their functional diversification into useful fine chemicals. Regioselective glucosylation of the secondary hydroxyl in different 1,2-propanediols was achieved by a sucrose phosphorylase-catalyzed tr...

Glycosylglycerols are powerful osmolytes, produced by various plants, algae and bac-teria in adaptation to salt stress and drought. Among them, glucosylglycerol (2-O-a-D-glu-copyranosyl-sn-glycerol; GG) has attracted special attention for its promising application as a moisturizing agent in cosmetics. A biocatalytic process for the synthesis of GG...

Compatible solutes constitute a diverse class of low-molecular-mass organic molecules that are accumulated in high intracellular concentrations in response to the external stress of hyperosmolality or high temperature. Many of these compounds like alpha, alpha-trehalose are well known for their stabilizing effect on protein structure and could lead...

Abstract Sucrose phosphorylase is a bacterial transglucosidase that catalyzes conversion of sucrose and phosphate into α-D-glucose-1-phosphate and D-fructose. The enzyme utilizes a glycoside hydrolase-like double displacement mechanism that involves a catalytically competent β-glucosyl enzyme intermediate. In addition to reaction with phosphate, gl...

Glycosylglycerols are powerful osmolytes, produced by various plants, algae and bacteria in adaptation to salt stress and drought. Among them, glucosylglycerol (2-O-α-D-glucopyranosyl-sn-glycerol; GG) has attracted special attention for its promising application as a moisturizing agent in cosmetics. A biocatalytic process for the synthesis of GG as...

Regioselective glucosylation of R-glycerate catalysed by sucrose phosphorylase in the presence of sucrose as the donor substrate provided the natural compatible solute (R)-2-O-alpha-D-glucopyranosyl glycerate with complete regioselectivity in an optimised synthetic yield of 90% R-glycerate converted and a concentration of about 270 mM.

(Figure Presented) Fit for industry: Stereochemically pure 2-O-(α-D-glucopyranosyl)-sn-glycerol (αGG) was obtained in high yield from an efficient and selective biocatalytic process (see schematic outline). The sucrose phosphorylase catalyzed transfer of a glucosyl group from sucrose to glycerol unites the main advantages of transglycosidases, glyc...

Stereochemisch reines 2-O-(α-D-Glucopyranosyl)-sn-glycerin (αGG) wird in hohen Ausbeuten nach einem effizienten und selektiven biokatalytischen Prozess erhalten. Der durch Saccharose-Phosphorylase katalysierte Transfer eines Glucosylrests auf Glycerin vereint die wichtigsten Vorteile der Glycosidsynthese mit Transglycosidasen, Glycosyltransferasen...

Tuna waste was used as a fortified amino acid source for black tiger shrimp larvae feed. It was hydrolysed by mixed culture of Lactobacillus brevis LB43 and L. plantarum LP64, which grew rapidly in the waste mixed with 10 percent molasses. The hydrolysate was added to commercial shrimp larvae feed at 12.5, 25.0 and 50.0 percent (v/w). The effect of...