Thomas Rolain

Thomas Rolain
Syneos Health on behalf of VBI Vaccines · Medical Affairs

PhD

About

35
Publications
3,882
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
675
Citations
Additional affiliations
October 2007 - July 2013
Université Catholique de Louvain - UCLouvain
Position
  • PhD Student

Publications

Publications (35)
Article
Full-text available
Peptidoglycan (PG) is an essential lattice of the bacterial cell wall that needs to be continuously remodeled to allow growth. This task is ensured by the concerted action of PG synthases that insert new material in the pre-existing structure and PG hydrolases (PGHs) that cleave the PG meshwork at critical sites for its processing. Contrasting with...
Data
Structural prediction and phylogenic relationships of NlpC/P60 catalytic domains.
Data
Effect of LytB inactivation on Z rings positioning.
Data
Cell cycle of the double LytA LytB deficient strain.
Data
RP-HPLC separation of muropeptides from L. plantarum WT and PnisA-lytA mutant (without, N0; and with nisin, N25).
Data
Effect of LytA depletion on septum misplacement in dividing cells.
Data
Time-lapse microscopy of ΔlytB mutant cells showing lysis of small non-growing cells.
Data
Importance of accessory LytA domains for its morphogenic function.
Data
Effect of the individual deletion of LytA domains on cell morphology.
Data
Impact of LytC and LytD depletion on cell morphology and growth.
Data
Effect of LytA depletion on septum misplacement in cell aggregates.
Data
Effect of LytB inactivation on septum misplacement in dividing long cells.
Data
Cell cycle of the conditional mreB1CD mutant (MCD203, N0).
Data
Cell cycle of the double LytA-LytB deficient strain (MCD20215, N0), Cell 1.
Data
Disaccharide (Ds)-peptide composition of PG from L. plantarum WT and PnisA-lytA mutant (without, N0; and with nisin, N25).
Data
Cell cycle of the conditional lytA mutant (MCD202, N0).
Data
Cell cycle of the double LytA-LytB deficient strain (MCD20215, N0), Cell 2.
Data
Cell cycle of the ΔlytB mutant (TR0015), Cell 1.
Data
Cell cycle of the ΔlytB mutant (TR0015), Cell 2.
Article
Full-text available
Peptidoglycan (PG) is an essential lattice of the bacterial cell wall that needs to be continuously remodeled to allow growth. This task is ensured by the concerted action of PG synthases that insert new material in the pre-existing structure and PG hydrolases (PGHs) that cleave the PG meshwork at critical sites for its processing. Contrasting with...
Article
Full-text available
In this review we stress the differences between eukaryotes and bacteria with respect to their different cell cycles, replication mechanisms and genome organizations. One of the most basic and underappreciated differences is that a bacterial chromosome uses only one ori while eukaryotic chromosome uses multiple oris. Consequently, eukaryotic oris w...
Article
Full-text available
Acm2, the major autolysin of Lactobacillus plantarum WCFS1, was recently found to be O-glycosylated with N-acetylhexosamine, likely N-acetylglucosamine (GlcNAc). In this study, we set out to identify the glycosylation machinery by employing a comparative genomics approach to identify Gtf1 homologues, which are involved in fimbria-associated protein...
Article
Peptidoglycan hydrolases are bacterial secreted enzymes that cleave covalent bonds in the cell-wall peptidoglycan, thereby fulfilling major physiological functions during cell growth and division. Although the molecular structure and functional roles of these enzymes have been widely studied, the molecular details underlying their interaction with...
Article
Full-text available
Acm2, the major autolysin of Lactobacillus plantarum, is a tripartite protein. Its catalytic domain is surrounded by an O-glycosylated N-terminal region rich in Ala, Ser, and Thr (AST domain), which is of low complexity and unknown function, and a C-terminal region composed of five SH3b peptidoglycan (PG) binding domains. Here, we investigate the c...
Article
Full-text available
Until now, peptidoglycan O-acetyl transferases (Oat) were only described for their peptidoglycan O-acetylating activity and for their implication in the control of peptidoglycan hydrolases. In this study, we show that a Lactobacillus plantarum mutant lacking OatA is unable to uncouple cell elongation and septation. Wild-type cells showed an elongat...
Article
Full-text available
Background Lactobacillus plantarum is commonly used in industrial fermentation processes. Selected strains are also marketed as probiotics for their health beneficial effects. Although the functional role of peptidoglycan-degrading enzymes is increasingly documented to be important for a range of bacterial processes and host-microbe interactions, l...
Article
Full-text available
Lactobacillus rhamnosus GG (LGG) produces two major secreted proteins, designated here Msp1 (LGG_00324 or p75) and Msp2 (LGG_00031 or p40), which have been reported to promote the survival and growth of intestinal epithelial cells. Intriguingly, although each of these proteins shares homology with cell wall hydrolases, a physiological function that...
Article
Full-text available
The peptidoglycan (PG) of Lactobacillus plantarum contains amidated meso-diaminopimelic acid (mDAP). The functional role of this PG modification has never been characterized in any bacterial species, except for its impact on PG recognition by receptors of the innate immune system. In silico analysis of loci carrying PG biosynthesis genes in the L....
Article
Full-text available
Peptidoglycan (PG) N-acetyl muramic acid (MurNAc) O-acetylation is widely spread in Gram-positive bacteria and is generally associated with resistance against lysozyme and endogenous autolysins. We report here the presence of O-acetylation on N-acetylglucosamine (GlcNAc) in Lactobacillus plantarum PG. This modification of glycan strands was never d...
Article
Lactobacilli belong to the lactic acid bacteria, which play a key role in industrial and artisan food raw-material fermentation, including a large variety of fermented dairy products. Next to their role in fermentation processes, specific strains of Lactobacillus are currently marketed as health-promoting cultures or probiotics. The last decade has...