Thomas Papouin

Thomas Papouin
Washington University in St. Louis | WUSTL , Wash U · Neuroscience

PhD

About

27
Publications
3,765
Reads
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2,745
Citations
Citations since 2016
15 Research Items
1863 Citations
20162017201820192020202120220100200300
20162017201820192020202120220100200300
20162017201820192020202120220100200300
20162017201820192020202120220100200300
Additional affiliations
August 2016 - August 2018
Tufts University
Position
  • Professor
August 2012 - present
Tufts University
Position
  • PostDoc Position
October 2011 - June 2012
University of Bordeaux
Position
  • PostDoc Position
Education
September 2007 - December 2007
Institut Pasteur International Network
Field of study
  • Neuroscience
January 2006 - September 2006
McGill University
Field of study
  • Neuroscience
September 2004 - June 2008
Sorbonne Université
Field of study
  • Integrative Biology and Physiology

Publications

Publications (27)
Article
The activation of the N-methyl D-aspartate receptor (NMDAR) is controlled by a glutamate-binding site and a distinct, independently regulated, co-agonist-binding site. In most brain regions, the NMDAR co-agonist is the astrocyte-derived gliotransmitter D-serine. We found that D-serine levels oscillate in mouse hippocampus as a function of wakefulne...
Article
The activation of NMDA receptors (NMDARs) is conditioned by the binding of a co-agonist to a dedicated receptor binding site. It is now largely accepted that D-serine plays this role at many central synapses in the hippocampus, amygdala, hypothalamus, nucleus accumbens, and in prefrontal, visual, and somatosensory cortices. D-Serine has been found...
Article
Full-text available
Long-term potentiation (LTP) of synaptic transmission provides an experimental model for studying mechanisms of memory. The classical form of LTP relies on N-methyl-D-aspartate receptors (NMDARs), and it has been shown that astroglia can regulate their activation through Ca(2+)-dependent release of the NMDAR co-agonist D-serine. Release of D-serine...
Article
N-methyl-d-aspartate receptors (NMDARs) are located in neuronal cell membranes at synaptic and extrasynaptic locations, where they are believed to mediate distinct physiological and pathological processes. Activation of NMDARs requires glutamate and a coagonist whose nature and impact on NMDAR physiology remain elusive. We report that synaptic and...
Article
Full-text available
N-methyl d-aspartate receptors (NMDARs) exist in different forms owing to multiple combinations of subunits that can assemble into a functional receptor. In addition, they are located not only at synapses but also at extrasynaptic sites. There has been intense speculation over the past decade about whether specific NMDAR subtypes and/or locations a...
Preprint
Full-text available
The participation of astrocytes in brain computation was formally hypothesized in 1992, coinciding with the discovery that these glial cells display a complex form of Ca2+ excitability. This fostered conceptual advances centered on the notion of reciprocal interactions between neurons and astrocytes, which permitted a critical leap forward in uncov...
Article
Full-text available
Familial hemiplegic migraine is an episodic neurological disorder characterized by transient sensory and motor symptoms and signs. Mutations of the ion pump α2-Na/K ATPase cause familial hemiplegic migraine, but the mechanisms by which α2-Na/K ATPase mutations lead to the migraine phenotype remain incompletely understood. Here, we show that mice in...
Article
Bidirectional communication between neurons and astrocytes shapes synaptic plasticity and behavior. D-serine is a necessary co-agonist of synaptic N-methyl-D-aspartate receptors (NMDARs), but the physiological factors regulating its impact on memory processes are scantly known. We show that astroglial CB1 receptors are key determinants of object re...
Article
Obtaining acute brain slices for electrophysiology or amperometric recordings has become a routine procedure in most labs in the field of neuroscience. Yet, protocols describing the step by step process are scarce, in particular for routine acute preparations such as from the mouse hippocampus. Here we provide a detailed protocol for the dissection...
Article
D-serine is an atypical amino acid present in the mammalian body (most amino acids in the mammalian body are L-isomers) that is mostly known in neuroscience for its role as a co-agonist controlling the N-methyl D-aspartate receptor (NMDAR). D-serine levels are decreased in patients with schizophrenia and this is thought to mediate, at least in part...
Data
D-serine and glycine dose-response curves. Summary of Hill coefficient (nH), half maximal concentration (EC50) and n-values for the glycine/D-serine dose-response curves obtained on GluN2A- and GluN2B-NMDARs recombinants. p-Values (unpaired, two-tailed Student t-test) assess the difference between the EC50 obtained with D-serine and glycine. DOI: h...
Data
Co-agonist and NMDAR-subunit developmental switch. Summary of the effects of BsGO, RgDAAO, Ro25-6981 and zinc normalized to baseline (mean ± s.e.m) in slices obtained from rats at indicated ages (see Figure 5—figure supplement 1). n values are indicated as well as p-values (paired Student t-test) assessing the significance of the effect achieved co...
Data
Capillary electrophoresis measurements. Summary of the measurements of glutamate, glycine and D-serine content in slices at different ages. Values indicate average content (in nmoles per mg of tissue) ± s.e.m. p-Values indicate the level of significance (student t-test) between values in <P10 slices compared to values in adults. DOI: http://dx.doi....
Article
The subunit composition of synaptic NMDA receptors (NMDAR), such as the relative content of GluN2A- and GluN2B-containing receptors, greatly influences the glutamate synaptic transmission. Receptor co-agonists, glycine and D-serine, have intriguingly emerged as potential regulators of the receptor trafficking in addition to their requirement for it...
Article
Astrocytes intimately interact with synapses, both morphologically and, as evidenced in the past 20 years, at the functional level. Ultrathin astrocytic processes contact and sometimes enwrap the synaptic elements, sense synaptic transmission and shape or alter the synaptic signal by releasing signalling molecules. Yet, the consequences of such int...
Chapter
The N-methyl d-aspartate receptor (NMDAR) is a ligand-gated ion channel that binds the neurotransmitter glutamate. It was pharmacologically identified and differentiated from other ionotropic amino-acid receptors at excitatory synapses in the late 70s for it is activated by NMDA and not kainate. Due to its large calcium conductance, it is involved...
Article
Planar cell polarity (PCP) signaling is well known to play a critical role during prenatal brain development; whether it plays specific roles at postnatal stages remains rather unknown. Here, we investigated the role of a key PCP-associated gene scrib in CA1 hippocampal structure and function at postnatal stages. We found that Scrib1 is required fo...
Article
Full-text available
Significance Mushroom bodies are prominent structures of the insect brain that have been associated with the storage and retrieval of elemental forms of memory. We took advantage of the unique cognitive capabilities of honey bees to uncover a previously unidentified function for these structures. Because the honey bee is the only insect in which hi...
Article
Full-text available
Scribble (Scrib) is a key regulator of apicobasal polarity, presynaptic architecture, and short-term synaptic plasticity in Drosophila. In mammals, its homolog Scrib1 has been implicated in cancer, neural tube closure, and planar cell polarity (PCP), but its specific role in the developing and adult nervous system is unclear. Here, we used the circ...
Article
Full-text available
The absence of fragile X mental retardation protein results in the fragile X syndrome (FXS), a common form of mental retardation associated with attention deficit, autistic behavior, and epileptic seizures. The phenotype of FXS is reproduced in fragile X mental retardation 1 (fmr1) knockout (KO) mice that have region-specific altered expression of...
Article
NMDA receptors (NMDARs) are key players of synaptic transmission and plasticity, which are localized at synaptic but also extrasynaptic sites. Their activation requires the binding of glutamate and of a second (co)-agonist, glycine and/or D-serine. However, the respective contribution of these two amino acids in controlling NMDAR activity is unclea...

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Projects

Project (1)
Project
Understand the physiological determinants that regulate how much D-serine astrocytes make available to NMDARs at synapses over time.