About
14
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Introduction
Thomas does research in Virology.
Additional affiliations
Education
September 2014 - December 2017
September 2012 - September 2014
Pierre and Marie Curie University - Paris 6 and Ecole Normale Supérieure de Paris
Field of study
- Fundamental Virology
Publications
Publications (14)
In the dynamic dance of evolution, organisms are often faced with fluctuating environments to which adaptation through selection of traditional heritable genetic mutations can be limiting. In this study, we unveil a refined mechanism of non-heritable variability in a virus with a compact DNA genome. We discovered that the genome of the Junona coeni...
Despite tight genetic compression, viral genomes are often organized in functional gene clusters, a modular structure that might favor their evolvability. This has greatly facilitated biotechnological developments, such as the recombinant Adeno-Associated Virus (AAV) systems for gene therapy. Following this lead, we endeavored to engineer the relat...
Recent developments on extracellular vesicles (EVs) containing multiple virus particles challenge the rigid definition of non-enveloped viruses. However, how non-enveloped viruses hijack cell machinery to promote non-lytic release in EVs, and their functional roles, remain to be clarified. Here we used Bluetongue virus (BTV) as a model of a non-env...
Background:
Transmission of many viruses occurs by direct transmission during a close contact between two hosts, or by an indirect transmission through the environment. Several and often interconnected factors, both abiotic and biotic, determine the persistence of these viruses released in the environment, which can last from a few seconds to seve...
Bluetongue virus (BTV) is an arthropod-borne virus infecting livestock. Its frequent emergence in Europe and North America had caused significant agricultural and economic loss. BTV is also of scientific interest as a model to understand the mechanisms underlying non-enveloped virus release from mammalian and insect cells. The BTV particle, which i...
Recent developments on extracellular vesicles (EVs) containing multiple virus particles challenge the rigid definition of non-enveloped viruses. However, how non-enveloped viruses hijack cell machinery to promote non-lytic release in EVs, and their functional roles, remain to be clarified. Here we used Bluetongue virus (BTV) as a model of a non-env...
Methods for virus particle quantification represent a critical aspect of many virology studies. Although several reliable techniques exist, they are
either time-consuming or unable to detect small variations. Presented here is a protocol for the precise quantification of viral titer by analyzing
electrical impedance variations of infected cells in...
Members of the Reoviridae family assemble virus factories within the cytoplasm of infected cells to replicate and assemble virus particles. Bluetongue virus (BTV) forms virus inclusion bodies (VIBs) that are aggregates of viral RNA, certain viral proteins, and host factors, and have been shown to be sites of the initial assembly of transcriptionall...
Bluetongue virus (BTV) is an arthropod-borne virus that infects domestic and wild ruminants. The virion is a non-enveloped double-layered particle with an outer capsid that encloses a core containing the segmented double-stranded RNA genome. Although BTV is canonically released by cell lysis, it also exits non-lytically. In infected cells, the BTV...
The transmission routes of Influenza A viruses (IAVs) submit virus particles to a wide range of environmental conditions that affect their transmission. In water, temperature, salinity, and pH are important factors modulating viral persistence in a strain-dependent manner, and the viral factors driving IAV persistence remain to be described. We use...
The transmission routes of Influenza A viruses (IAVs) submit virus particles to a wide range of environmental conditions that affect their transmission. In water, temperature, salinity and pH are important factors modulating viral persistence in a strain-dependant manner, and the viral factors driving IAV persistence remained to be described. We us...
La transmission des virus grippaux de type A s’effectue via l’eau, l’air ou les surfaces. Elle implique donc toujours une étape dans l’environnement, durant laquelle les virus sont inactivés plus ou moins rapidement en fonction du sous-type ou de la souche virale analysés. Cependant, à ce jour, les facteurs moléculaires déterminant la stabilité des...
Questions
Questions (3)
We don't manage to subculture those cells in our lab. After thawing, we didn't observed any growth and their viability decreased slowly until death.
conditions of culture are:
- Incubator: 37,5°C, 5% Co2
- medium: Expi expression medium (provided by the supplier), pH measured = 7,5
- Cells were cultured in sterilized and cell culture treated erlenmeyer with vent caps. And they were on an orbital agitator at 110 RPM.
Thank you very much if you can provide some tips/informations about those cells.
MDCK cells are known to be trypsin resistant, and very long to detach from the dish. I read in some protocols that a trypsin (10x) could be used and would be more efficient. I think it could be a more aggresive, but a quicker treatment. It's a balance. Does anyone already tried a more concentrated trypsin for those cells?
We have difficulties to obtain high titers of a pandemic H1N1 influenza virus.
We use MDCK cells and A/Paris/2590/09 virus strain.
We already tried different MOI (10-3 and 10-4)