Thierry Noguer

Thierry Noguer
Université de Perpignan | UPVD · Faculty of Exact and Experimental Sciences

PhD

About

102
Publications
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Publications

Publications (102)
Article
This study focuses on the spectroelectrochemical quantification of four UV filters, butylmethoxy dibenzoylmethane (BM), benzophenone-3 (BP3), ethylhexyl methoxycinnamate (EM) and octocrylene (OC) used as sunscreens in cosmetics. Three of them exhibited electrochemical activity resulting in the modification of their absorption spectrum under the app...
Article
Full-text available
Chlortoluron (3-(3-chloro-p-tolyl)-1,1-dimethyl urea) is an herbicide widely used in substitution to isoproturon to control grass weed in wheat and barley crops. Chlortoluron has been detected in groundwaters for more than 20 years; and dramatic increases in concentrations are observed after intense rain outbreaks. In this context; we developed an...
Article
A novel dual electrochemical immunosensor was fabricated for the rapid and sensitive detection of enrofloxacin (EF) antibiotic in meat. Anti-quinolone antibody was immobilized onto screen-printed dual carbon electrodes via carbodiimide coupling. A new electrochemical probe was synthesized by conjugating difloxacin and aminoferrocene, whose oxidatio...
Article
Bistriazole ligand and its corresponding copper complexes were synthesized and characterized for the first time and proposed as new labels for the development of electrochemical aptasensors. The bistriazole ligand was prepared from methyl 1,6‐heptadiyne‐4‐carboxylate and 2‐(azidomethyl)phenol using classical CuAAC in presence of different copper sa...
Chapter
Biosensors can be defined as self-containing analytical devices comprised of a biological sensing element, or biorecognition element, responsible for specificity, and a physical transducer that converts the recognition phenomenon into a measurable signal. They can be classified according to the type of biological element involved. For instance, enz...
Article
Scanning electrochemical microscopy (SECM) is discussed as a versatile tool to provide a unique approach of localized electrochemical information in the context of biosensing research. The step‐by‐step immobilization of DNA aptamer with intrinsic redox activity on screen‐printed carbon electrode (SPCE) was successfully monitored using SECM imaging...
Article
The preparation of efficient molecularly imprinted polymers materials (MIPs) for pharmaceutical residue removal is still a challenging task. Herein, we design uniformly molecularly imprinted polymer nanospheres via a precipitation polymerization method using methacrylic acid (MAA) as functional monomer and N,N-methylenebis(acrylamide) (MBAA) as a c...
Article
This work presents the synthesis and characterization of salen/salan metal complexes for their future application as electrochemical labels in affinity sensors. Due to its stability and electrochemical properties, oxovanadium salan...
Article
Full-text available
Rapid detection of the foodborne pathogen Salmonella in food processing is of crucial importance to prevent food outbreaks and to ensure consumer safety. Detection and quantification of Salmonella species in food samples is routinely performed using conventional culture-based techniques, which are labor intensive, involve well-trained personnel, an...
Article
Cheliff River is the most important permanent river of North-west Algeria which plays an essential role for irrigation and drinking water supply, but suffers from high anthropogenic pressure. Multi-dimensional and multi-factorial aspects of water pollution in Cheliff River and its tributary Mina River were evidenced during two contrasting climatic...
Article
In biosensors and biofuel cells, it is often desirable to accelerate the electron transfer rate between the enzyme and electrode surface to improve the performance of the devices (sensitivity or...
Article
A label-free electrochemical aptasensor was fabricated for reagentless determination of tetracycline (TET) in water. A 5′-amino-modified 76-mer oligonucleotide was immobilized on screen-printed carbon electrode via carbodimide coupling. Cyclic voltammetry experiments revealed an unexpected electroactivity of immobilized aptamer, showing a quasi-rev...
Article
An ultrasensitive label-free amperometric immunosensor for the detection of chloramphenicol (CAP) residues in milk has been developed by using a screen-printed carbon electrode laminated with a layer of poly (vinyl alcohol-co-ethylene) (PVA-co-PE) nanofibrous membrane that is covalently immobilized with a CAP antibody (anti-CAP). The performance of...
Article
p>BIOFOS aims to further develop and validate a reusable and high-added value Lab-on-Chip (LoC) based, micro-biophotonic sensor platform for in situ monitoring of food contaminants. The Lab-on-Chip was tested on milk (aflatoxin M1, antibiotics and lactose), olive oil (pesticides and metals), nuts (aflatoxin B1) and dehydrated fruits (ochratoxin A)....
Article
Electrochemical molecularly imprinted polymers (e-MIPs) were for the first time introduced in screen-printed carbon electrodes (SPCE) as the sensing element for the detection of an organic pollutant. To play this sensing role, a redox tracer was incorporated inside the binding cavities of a cross-linked MIP, as a functional monomer during the synth...
Article
Full-text available
One of the main pursuits, yet most difficult, in monitoring studies is to identify the sources of environmental pollution. In this study, we have identified health-care facilities from south European countries as an important source of pharmaceuticals in the environment. We have estimated that compounds consumed in by the elderly and released from...
Article
New chemiluminescence-based immunoassays for sensitive detection of 17-β estradiol (E2) and ethinylestradiol (EE2) are described on the basis of the use of biotinylated estrogen derivatives. Estrogen derivatives bearing a carboxylic group (E2-COOH and EE2-COOH) on C-3 position were synthesized, covalently bound to aminated biotin and subsequently i...
Article
Molecularly imprinted polymers (MIPs) for phosmet insecticide were synthesized by batch polymerization. The affinity of functional monomers to phosmet was tested using an original method involving an electrochemical biosensor based on acetylcholinesterase inhibition. It was demonstrated that association of phosmet with appropriate functional monome...
Article
This paper describes for the first time the selection of aptamers selective to penicillin. Aptamers were selected using a specific process called Capture-SELEX (Systematic Evolution of Ligands by Exponential Enrichment). This technique is based on the selection of DNA aptamers using penicillin G in solution while the ssDNA library is fixed on a sup...
Article
An amperometric biosensor was designed for the determination of sulcotrione, a β-triketone herbicide, based on inhibition of hydroxyphenylpyruvate dioxygenase (HPPD), an enzyme allowing the oxidation of hydroxyphenylpyruvate (HPP) in homogentisic acid (HGA). HPPD was produced by cloning the hppd gene from Arabidopsis thaliana in E. coli, followed b...
Article
This paper describes the development of an amperometric biosensor based on galactose oxidase (GAOx) immobilization within a laponite clay film deposited on Carbon Screen-Printed Electrodes modified by electrodeposited Prussian Blue and coated with poly-(O-phenylenediamine) (PPD/PB/CSPEs). Amperometric performances of GAOx@laponite/PPD/PB/CSPEs bioe...
Article
We report here a novel method to detect methidathion organophosphorous insecticides. The sensing platform was architected by the combination of molecularly imprinted polymers and sol-gel technique on inexpensive, portable and disposable screen printed carbon electrodes. Electrochemical impedimetric detection technique was employed to perform the la...
Article
Full-text available
Immobilization of a low amount of electric eel acetylcholinesterase on microtiter plates by bioencapsulation in a sol-gel composite led to the preparation of a simple colorimetric enzymatic-assay for pesticides detection in olive oil. This bioassay has been used to carry out inhibition studies with three pesticides mainly used in the treatment of o...
Article
This review summarises the main electrochemical sensors and biosensors developed for assessing the reactive oxygen (ROS) and nitrogen (RNS) species of interest in clinical and physiological analysis. Among these species, a special attention is focused on sensors for the detection of the highly reactive superoxide radicals O2.−, the very stable hydr...
Article
Full-text available
Plant 4-hydroxyphenylpyruvate dioxygenase (HPPD) is the molecular target of a range of synthetic β-triketone herbicides that are currently used commercially. Their mode of action is based on an irreversible inhibition of HPPD. Therefore, this inhibitory capacity was used to develop a whole-cell colorimetric bioassay with a recombinant Escherichia c...
Article
In this work we present a rapid, selective, and highly sensitive detection of α-solanine and α-chaconine using cholinesterase-based sensors. The high sensitivity of the devices is brought by the use of a genetically-modified acetylcholinesterase (AChE), combined with a one-step detection method based on the measurement of inhibition slope. The sele...
Article
Full-text available
Oxovanadium complexes are presented as new labels for the development of electrochemical immunosensors. The concept was successfully applied to the accurate detection of estradiol, an emerging environmental pollutant, at concentrations ranging from 4 ng L(-1) to 5 μg L(-1).
Article
Novel electrochemical immunosensors for sensitive detection of 17-β estradiol (E2) and ethinylestradiol (EE2) are described based on the use of magnetic beads (MBs) as solid support and screen-printed electrodes as sensing platforms. Four synthetic estrogen derivatives containing either a carboxylic group or an amine group at the C-3 position were...
Article
This work presents the development of molecularly imprinted polymers (MIPs) for the selective extraction of dimethoate from olive oil. Computational simulations allowed selecting itaconic acid as the monomer showing the highest affinity towards dimethoate. Experimental validation confirmed modelling predictions and showed that the polymer based on...
Article
Full-text available
This work presents the development of bioassays and biosensors for the detection of insecticides widely used in the treatment of olive trees. The systems are based on the covalent immobilisation of acetylcholinesterase on magnetic microbeads using either colorimetry or amperometry as detection technique. The magnetic beads were immobilised on scree...
Article
This paper presents the construction of amperometric biosensors for the detection of organophosphorus insecticides widely used in the treatment of olive trees. The systems are based on the immobilisation of acetylcholinesterase on screen-printed electrodes by bioencapsulation in a solegel composite. The enzyme activity was estimated by measuring th...
Chapter
Due to their high acute toxicity and risk towards the population, some directives have been established to limit the presence of pesticides in water and food resources. It is thus mandatory to develop efficient decontamination techniques in combination with highly sensitive detection techniques allowing to accurately determine the level of contamin...
Article
Reactive oxygen species (ROS) are very unstable molecules generated during the metabolism. They can be produced in excess, contributing to cellular dysfunctions. Antioxidants are substances that play an important role as a health-protecting factor, limiting the lesions caused by ROS. Two kinds of electrochemical biosensors based on ROS have been de...
Article
A specific adsorbent for extraction of methidathion from olive oil was developed. The design of the molecularly imprinted polymer (MIP) was based on the results of the computational screening of the library of polymerisable functional monomers. MIP was prepared by thermal polymerisation using N,N'-methylene bisacrylamide (MBAA) as a functional mono...
Article
Poly(3,4-ethylenedioxythiophene) (PEDOT) and its derivatives are relatively new, and unique members of conducting-polymers family. In this article, we present an approach for simple, reliable and cost-efficient electrochemical biosensor for real-time detection and quantification of phenolic compounds (PhCs). The PEDOT:poly(styrene sulfonate) (PSS)...
Article
a b s t r a c t We present a one-step inhibition biosensor method that can be used to determine mixtures of three organophosphorus compounds. We propose to calculate the rate of irreversible inactivation of the enzyme (k p) to demonstrate the direct relationship between enzymatic biosensors and Chlorpyriphos-oxon (CPO), Chlorfenvinphos (CFV) and Az...
Article
This work describes the use of layered double hydroxides (LDHs) for the immobilisation of acetylcholinesterase (AChE) on insulator/semiconductor solid supports. Different LDHs have been synthesised by a co-precipitation method. Afterwards, biohybrid materials based on AChE-LDH mixtures have been produced using wild and recombinant enzymes. Spectros...
Article
This paper reports site-specific affinity immobilization of (His)6-tagged acetylcholinesterase (AChE) onto Ni/NiO nanoparticles for the development of an electrochemical screen-printed biosensor for the detection of organophosphate pesticides. The method is based on the specific affinity binding of the His-tagged enzyme to oxidized nickel nanoparti...
Article
A novel electrochemical aptasensor, based on disposable screen-printed electrodes was developed for the sensitive detection of the mycotoxin ochratoxin A (OTA). Two strategies were investigated by using an indirect and a direct competitive assay, based on the use of superparamagnetic nanoparticles. The performance of the optimized aptasensors in te...
Article
Full-text available
A poly(3,4-ethylenedioxythiophene) (PEDOT) conducting ink is presented as a new electroactive material to be incorporated in acetylcholinesterase-(AChE-) based screen printed biosensors, acting not only as a conducting template but also as an electrochemical mediator for thiocholine oxidation. Two different strategies have been studied for the chem...
Article
Full-text available
This work describes the use of a PEDOT:PSS-based conductive polymer for designing AChE-based biosensors. The transducers were obtained directly by screen-printing a PEDOT:PSS suspension on the surface of thick film carbon electrodes. The obtained working electrodes showed a high conductivity when compared with electrodes modified with conventional...
Article
This work presents a hardware implementation of artificial neural networks (ANNs) using a dsPIC microcontroller to resolve mixtures of pesticides measured by amperometric acetylcholinesterase (AChE) biosensors. The response of three biosensors with different concentrations of Chlorpyrifos Oxon (CPO) and Chlorfenvinfos (CFV) was modeled by two ANNs,...
Article
Enzyme spectrophotometric assays based on acetylcholinesterase (AChE) inhibition were used in combination with Artificial Neural Network (ANN) chemometric analysis for the resolution of pesticides mixtures of chlorpyriphos, dichlorvos and carbofuran. Electric eel (EE) AChE and the recombinant B394-AChE from Drosophila melanogaster were selected due...
Chapter
Full-text available
Acetyl- and butyl-cholinesterases have been described for many years as sensitive tools for the detection of many neurotoxic compounds such as insecticides (OPs and carbamates), chemical weapons and toxins (anatoxin-a(s)). They have been extensively used in biosensor configurations based on amperometric detection. Basically, the first devices descr...
Article
The food industries need rapid and affordable methods to assure the quality ofproducts and process control. Biosensors, combining a biological recognition element and a sensitive transducer, are versatile analytical tools that offer advantages as classical analytical methods due to their inherent specificity, selectivity and simplicity. This paper...
Article
This works presents the development of a detoxification system based on bacterial phosphotriesterase (PTE) for the degradation of organophosphate (OP) insecticides in water. PTE was immobilised on an activated agarose gel via covalent coupling. Two different OPs were studied, chlorpyrifos and chlorfenvinfos, due to their importance in the field of...
Article
Amperometric acetylcholinesterase (AChE) biosensors have been developed to resolve mixtures of chlorpyrifos oxon (CPO) and chlorfenvinfos (CFV) pesticides. Three different biosensors were built using the wild type from electric eel (EE), the genetically modified Drosophila melanogaster AChE B394 and B394 co-immobilized with a phosphotriesterase (PT...
Article
This work shows the possibility of combining the high sensitivity of genetically-modified Drosophila melanogaster acetylcholinesterase (B394) with the ability of phosphotriesterase (PTE) to hydrolyse organophosphate compounds, in the aim of developing a biosensor selective to two insecticides of interest: chlorpyrifos and chlorfenvinfos. The studie...
Article
Screen-printed electrodes modified with appropriate mediators have been used for the reliable determination of dithiocarbamate fungicides and alkylphenols. A sensor based on immobilization of aldehyde dehydrogenase on the surface of a disposable screen-printed carbon-paste electrode, chemically modified with Meldola’s blue mediator, was used for th...
Article
A stick type biochemical gas sensor (bio-sniffer) with formaldehyde dehydrogenase (FALDH) was developed for convenient analysis of gaseous formaldehyde with high gas selectivity. The bio-sniffer for formaldehyde in the gas phase was constructed by immobilizing enzyme to a Pt electrode-coated hydrophilic PTFE membrane. The oxidation current of NADH...
Article
The increasing concern about ochratoxin A (OTA) contamination of different food and feedstuffs demands high-performing detection techniques for quality assessment. Two indirect competitive enzyme-linked immunosorbent assay (ELISA) strategies were investigated for the development of OTA electrochemical immunosensors based on different OTA immobilisa...
Article
A disposable bienzymic sensor for the reliable determination of dithiocarbamate fungicides was developed. Aldehyde dehydrogenase and a highly stable NADH oxidase were immobilized on the surface of a disposable Pt-sputtered carbon paste screen printed electrode by using photocrosslinkable poly(vinylalcohol) bearing styrylpyridinium groups. Due to th...
Article
Amperometric biosensors based on NADHox/FMN and Prussian blue modified screen-printed electrodes (SPE) have been developed in order to improve the NADH detection. The catalytic effect, the sensitivity and the stability of PB-modified SPEs were studied for different pH buffer solutions and different voltage range, by cyclic voltammetry and amperomet...
Article
Full-text available
The inhibition sensitivity of wild and mutant acetylcholinesterases (AChE) towards selected organophosphorus pesticides has been compared with enzymes immobilized in a photocured layer of polyvinylalcohol polymer (PVA-AWP) on a screen-printed graphite electrode. The investigated pesticides included the widely used malaoxon (MO), chlorfenvinphos (CF...
Article
This work presents a biosensor for organophosphorus pesticides based on immobilisation of a highly sensitive genetically engineered acetylcholinesterase (B394) by affinity interactions on metal chelate-functionalised magnetic microbeads. The developed sensor has been compared with those based on the widely used Electric eel cholinesterase and a cla...
Article
Mycotoxins are secondary metabolites that moulds produce naturally. Due to their ubiquitous presence in foodstuffs and their potential risk for human health, prompt detection is important. In this review, we present and critically compare recent advances in mycotoxin analysis. Although most validated detection methods are chromatographic, alternati...
Article
In this study three fluorescent probes were compared, namely the simple Mag-Indo-1, the new ochratoxin A (OTA) and the classical eosin (EOS). Mag-Indo-1 binds to bovine serum albumin (BSA) with a high binding constant (Kd = 1.8 10-7 M), at a single binding site in an equimolar ratio. Low-specific or multiple binding is not observed. OTA binds to BS...
Article
Full-text available
Initially, all major factors that affect the rate of the AldH-catalyzed reaction (enzyme concentration, substrate concentration, temperature and pH) were investigated. Optimal activity was observed between pH values of 7.5 and 9.5 in the temperature range of 25 to 50 ºC. Kinetic parameters, such as Km (2.92 µmol L-1) and Vmax (1.33 10-2µmol min-1)...
Chapter
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