Thierry Burnouf

Taipei Medical University | TMU · College of Biomedical Engineering

Microvesicles (MVs) are subcellular physiological vehicles present in all body fluids, mediate the transfer of intercellular information within biological systems, and contribute to healthy conditions. MVs have lipid bilayer membranes decorated with multiple ligands that can interact with receptors on target cells, rendering them promising for targ...

There is much interest in the use of extracellular vesicles (EVs) as a subcellular therapy for regenerative medicine and drug delivery. Blood-borne platelets represent a source of therapeutic EVs that is so far largely unexplored. Advantages of platelets as a cellular source of EVs include their established clinical value, regulated collection proc...

Free link to the PDF until Sept 5, 2017: https://authors.elsevier.com/a/1VOtFWWN0alX7 Human platelet lysates (PLs), which contain multiple neurotrophins, have been proposed for treating neurodegenerative disorders, including Parkinson’s disease (PD). However, current PLs suspended in plasma have high protein content and contain fibrinogen/fibrin...

Background: Human platelets (PLT) and PLT-extracellular vesicles (PEV) released upon thrombin activation express receptors that interact with tumour cells and, thus, can serve as a delivery platform of anti-cancer agents. Drug-loaded nanoparticles coated with PLT membranes were demonstrated to have improved targeting efficiency to tumours, but rema...

Platelets are small anucleated blood cells primarily known for their vital hemostatic role. Allogeneic platelet concen‑ trates (PCs) collected from healthy donors are an essential cellular product transfused by hospitals to control or pre‑ vent bleeding in patients affected by thrombocytopenia or platelet dysfunctions. Platelets fulfill additional...

Impaired wound healing is a significant complication of diabetes. Platelet-derived extracellular vesicles (pEVs), rich in growth factors and cytokines, show promise as a powerful biotherapy to modulate cellular proliferation, angiogenesis, immunomodulation, and inflammation. For practical home-based wound therapy, however, pEVs should be incorporat...

Use of platelet-derived extracellular vesicles as nanosized drug delivery vehicles of anti-cancer agents

Systemic inflammation, neurovascular dysfunction, and coagulopathy often occur concurrently in neuropathologies. Neutrophils and platelets have crucial synergistic roles in thromboinflammation and are increasingly suspected as effector cells contributing to the pathogenesis of neuroinflammatory diseases. In this review, we summarize the roles of pl...

Background: Systemic therapy is the primary treatment for advanced thymic malignancies. However, there is an urgent need to improve clinical outcome. Personalized treatment based on predictive biomarkers is a potential approach to address this requirement. In this study, we aimed to show the correlation between drug sensitivity tests on CTCs-deriv...

Due to the mortality associated with thrombosis and its high recurrence rate, there is a need to investigate antithrombotic approaches. Noninvasive site-specific thrombolysis is a current approach being used; however, its usage is characterized by the following limitations: low targeting efficiency, poor ability to penetrate clots, rapid half-life,...

e23521 Background: Pharmacologic treatment for soft tissue sarcoma (STS) remains challenging. There is a lack to predict treatment responses to chemotherapy or targeted therapy to oncologic drivers. We hypothesize that circulating tumor cells may enrich cancer initiating cells and represent a window to probe personalized drug treatment response. In...

Twenty-one gliomas in patients aged 0-21 years were evaluated for drug sensitivity by ex vivo expanded circulating tumor cells (CTC). The results were correlated with clinical outcomes. Venous blood samples were obtained prior to drug treatment. Peripheral blood mononuclear cells were processed in a 3D cell culture system (EVA Select™, Cancer Free...

Background and Objectives: Serum eye drops (SEDs) are used to treat ocular surface disease (OSD) and to promote ocular surface renewal. However, their use and pro- duction are not standardized, and several new forms of human eye drops have been developed. Materials and Methods: The International Society for Blood Transfusion Working Party (ISBT WP)...

This article explains the expected value of polyvalent plasma-derived immunoglobulins currently produced from healthy blood or plasma donors in protecting immunocompromised individuals against the risk of serious COVID-19 infections

Classically, patients with solid and hematologic malignancies have been treated with a combination of chemotherapy with or without a holistic targeted strategy using approved conventional therapy. While the evidence-based use of Immunomodulatory drugs and Immune checkpoint inhibitors (ICIs), including those targeting the PD-1, PD-L1 and CTLA-4, hav...

Paraproteins occasionally result in life-threatening hyperviscosity syndrome that causes harmful effects on various body tissues. These effects, despite therapy, can be irreversible. A clone-directed approach to paraproteinemia aims at quickly suppressing paraprotein production.Non-pharmacological therapies such as plasmapheresis eliminate paraprot...

Therapeutic blood products including convalescent plasma/serum and immunoglobulins concentrated from convalescent plasma, such as intravenous immunoglobulins or hyperimmune globulins, and monoclonal antibodies are passive immunotherapy options for novel coronavirus disease 2019 (COVID-19). They have been shown to improve the clinical status and bio...

Autoimmune rheumatic disorders (ARD) represent a wide spectrum of disorders that affect in priority the joints, bones, muscles, and connective tissues. Examples of ARD include rheumatoid arthritis, systemic lupus erythematosus, Sjögren syndrome, polymyositis, systemic sclerosis, antiphospholipid syndrome and mixed connective tissue disease. Patient...

This preprint provides a commentary and further elaboration on the conclusions reached during a recent international workshop on plasma protein therapies organized by the Working Party for Global Safety of the International Society of Blood Transfusion (ISBT). The workshop addressed the profound deficiency in access to safe plasma protein therapies...

Platelets, the small, anucleate blood cells that originate from megakaryocytes in the bone marrow, are typically associated with coagulation. However, it is now apparent that platelets are more multifaceted than originally thought, with their function extending beyond their traditional role in hemostasis to acting as important mediators of brain fu...

Corneal endothelial cells (CECs) slowly decrease in number with increasing age, which is a clinical issue as these cells have very limited regenerative ability. Therapeutic platelet biomaterials are increasingly used in regenerative medicine and cell therapy because of their safety, cost-effective manufacture, and global availability from collected...

Brain administration of human platelet lysates (HPL) is an emerging biotherapy of neurodegenerative and traumatic diseases of the central nervous system (CNS). HPLs being prepared from pooled platelet concentrates (PCs) increasing viral risks, manufacturing processes should incorporate robust virus‐reduction treatments. We evaluated a 19±2‐nm virus...

Neurodegenerative disorders of the central nervous system (CNS) and brain traumatic insults are characterized by complex overlapping pathophysiological alterations encompassing neuroinflammation, alterations of synaptic functions, oxidative stress, and progressive neurodegeneration that eventually lead to irreversible motor and cognitive dysfunctio...

The coronavirus disease 2019 (COVID-19) pandemic has highlighted the potential therapeutic value of early passive polyclonal immunotherapy using high-titer convalescent plasma (CCP). Human polyclonal hyperimmune immunoglobulin (HIG) has several advantages over CCP. Unlike CCP, HIG can provide standardized and controlled antibody content. It is also...

The coronavirus disease 2019 (COVID-19) pandemic has highlighted the potential therapeutic value of early passive polyclonal immunotherapy using high-titer convalescent plasma (CCP). Human polyclonal hyperimmune immunoglobulin (HIG) has several advantages over CCP. Unlike CCP, HIG can provide standardized and controlled antibody content. It is also...

Background: Outdated allogenic platelet concentrates (PCs) from blood establishments can be used as source material for the preparation of different types of lysates (HPLs) for clinical applications in cell therapy and regenerative medicine. It is important to understand how the substantial variations in the mode of preparation of HPLs can affect t...

The COVID-19 pandemic caused by the SARS-CoV-2 virus has significantly disrupted and burdened the diagnostic workup and delivery of care, including transfusion, to cancer patients across the globe. Furthermore, cancer patients suffering from solid tumors or hematologic malignancies were more prone to the infection and had higher morbidity and morta...

Introduction Pancreatic ductal adenocarcinoma (PDAC) is highly aggressive and has poor prognosis. There are few biomarkers to inform treatment decisions, and collecting tumour samples for testing is challenging. Methods Circulating tumour cells (CTCs) from patients with PDAC liquid biopsies were expanded ex vivo to form CTC-derived organoid cultur...

Platelets are at the crossroads between thrombosis and inflammation. When activated, platelets can shed bioactive extracellular vesicles [pEVs] that share the hemostatic potential of their parent cells and act as bioactive shuttles of their granular contents. In a viral infection, platelets are activated, and pEVs are generated with occasional viri...

Background and Objectives: The coronavirus disease 2019 (COVID-19) pandemic has impacted blood systems worldwide. Challenges included maintaining blood supplies and initiating the collection and use of COVID-19 convalescent plasma (CCP). Sharing information on the challenges can help improve blood collection and utilization. Materials and Methods:...

Convalescent plasma (CP) recurs as a frontline treatment in epidemics because it is available as soon as there are survivors. The COVID-19 pandemic represented the first large-scale opportunity to shed light on the mechanisms of action, safety, and efficacy of CP using modern evidence-based medicine approaches. Studies ranging from observational ca...

Background and objectives: The coronavirus disease 2019 (COVID-19) pandemic has impacted blood systems worldwide. Challenges included maintaining blood supplies and initiating the collection and use of COVID-19 convalescent plasma (CCP). Sharing information on the challenges can help improve blood collection and utilization. Materials and methods...

Background and objectives: Actions are needed to improve access to safe plasma-derived medicinal products (PDMPs) in low- and middle-income countries (LMICs). Materials and methods: The International Society of Blood Transfusion (ISBT) Working Party for Global Blood Safety organized an on-line workshop during 21-23 September 2021 to advance acce...

Background and Objectives The coronavirus disease 2019 (COVID-19) pandemic has impacted blood systems worldwide. Challenges included maintaining blood supplies and initiating the collection and use of COVID-19 convalescent plasma (CCP). Sharing information on the challenges can help improve blood collection and utilization. Materials and Methods...

Platelets have unique properties that can be used in a variety of clinical settings. Their ability to target and accumulate in the lung cancer microenvironment can be exploited as a therapeutic targeted delivery method. In this study, we developed a drug-free biomimetic platelet-polyethyleneimine polypyrrole (PLT-PEI-PPY) conjugate that expresses e...

Published by Transfusion - https://onlinelibrary.wiley.com/share/author/SGRRT7AJSR7786NSCCJG?target=10.1111/trf.16810 Convalescent plasma (CP) has been used to treat emerging infectious diseases for over a century. It was notably deployed during the 1918 flu pandemic and has been explored during numerous disease outbreaks, including those caused by...

There is concrete evidence that outdated allogeneic platelet concentrates not used for transfusion are valuable sources of functional trophic factors, including growth factors, cytokines, chemokines, anti-inflammatory and antioxidant molecules. Such outdated platelet concentrates can be used to produce human platelet lysates (HPL), a proven potent...

The advanced-stage head and neck cancer (HNC) patients respond poorly to platinum-based treatments. Thus, a reliable pretreatment method for evaluating platinum treatment response would improve therapeutic efficiency and outcomes. This study describes a novel strategy to predict clinical drug responses in HNC patients by using eSelect, a lab-develo...

Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease of motor neurons leading to death within 3 years and without a curative treatment. Neurotrophic growth factors (NTFs) are pivotal for cell survival. A reason for the lack of patient efficacy with single recombinant NTF brain infusion is likely to be due to the synergisti...

Human platelet lysate (HPL) is a complex mixture of potent bioactive molecules instrumental in tissue repair and regeneration. Due to their remarkable safety, cost-effective production, and availability at global level from collected platelet concentrates, HPLs can become a powerful biotherapy for various therapeutic applications, if standardized a...

PDF of presentations and recording are freely available on the ISBT website (Working Party for Global Blood Safety): https://www.isbtweb.org/working-parties/global-blood-safety/workshop-recordings

Convalescent plasma (CP) recurs as a frontline treatment in epidemics because it is available as soon as there are survivors. The COVID-19 pandemic represented the first large-scale opportunity to shed light into mechanisms of action, safety and efficacy of convalescent plasma using modern evidence-based medicine approaches. Studies ranging from ob...

Background Pancreatic ductal adenocarcinoma (PDAC) is highly aggressive and has poor prognosis. There are few biomarkers to inform treatment decisions, and collecting tumor samples for genomic or drug sensitivity testing is challenging.Methods Circulating tumor cells (CTCs) were prepared from the liquid biopsies of PDAC patients. These cells were s...

COVID-19 convalescent plasma (CCP) is currently under investigation for both treatment and post-exposure prophylaxis. The active component of CCP mediating improved outcome is commonly reported as specific antibodies, particularly neutralizing antibodies, with clinical efficacy characterized according to the level or antibody affinity. In this revi...

Background: Plasma-derived immunoglobulins (IgG) are essential medicines that are in worldwide shortage, especially in low- and middle-income countries. Optimised manufacturing processes can increase supply. We evaluated various new process steps for IgG fractionation. Material and methods: A crude, worst-case, IgG intermediate obtained by capry...

Nanoparticle–Protein Corona Neural Interfaces In article number 2100694, Po-Chun Chen, Wei-Chen Huang, and co-workers develop a smart biotic-abiotic nano-assembly neural interface to provide controllable topographical, electrical, and chemical stimuli that can enhance neuronal activity. In the nanoparticle (NP)–protein corona structures, the soft N...

Iridium oxide (IrOx) is a promising implantable electrode material owing to its remarkable neural stimulation capacity. However, presently, IrOx electrodes lack biocompatibility and bioactive interactions with nerve tissues. Application of polymeric surface coatings results in a weak physical adhesion at the organic/inorganic interface, which limit...

Background aims Platelet concentrates (PCs) are pooled to prepare human platelet lysate (HPL) supplements of growth media to expand primary human cells for transplantation; this increases the risk of contamination by known, emerging, and unknown viruses. This possibility should be of concern because viral contamination of cell cultures is difficult...

Traumatic brain injury leads to major brain anatomopathological damages underlined by neuroinflammation, oxidative stress and progressive neurodegeneration, ultimately leading to motor and cognitive deterioration. The multiple pathological events resulting from traumatic brain injury can be addressed not by a single therapeutic approach, but rather...

Free access to the full text: https://onlinelibrary.wiley.com/doi/full/10.1111/vox.13114

Free access to the full text: https://onlinelibrary.wiley.com/doi/full/10.1111/vox.13113

Background The lack of definitive treatment or preventative options for COVID‐19 led many clinicians early on to consider convalescent plasma (CCP) as potentially therapeutic. Regulators, blood centres and hospitals worldwide worked quickly to get CCP to the bedside. Although response was admirable, several areas have been identified to help impro...

Vascular leiomyosarcoma is an extremely rare tumor and is associated with poor prognosis among leiomyosarcoma. Surgical resection remains the main treatment option. But outcome of definitive treatment with chemoradiotherapy in inoperable patients is not clear. Here, we report treatment and outcome of definitive chemoradiotherapy in a case of vascul...

This article reviews how human platelet lysate can be used in place of FBS to expand human cells ex vivo: A pathogen-free and standardized xeno-free supplement of growth media is required for the ex vivo propagation of human cells used as advanced therapeutic medicinal products and for clinical translation in regenerative medicine and cell therapie...

Human platelet lysates (HPLs), rich in various growth factors and cell growth-promoting molecules, encompass a new range of blood products that are being used for regenerative medicine, cell therapies, and tissue engineering. Well-characterized dedicated preparations, tailor-made to best fit specific therapeutic applications, are needed for optimal...

Small cell lung cancer (SCLC) represents one of the most aggressive malignancies among cancer types. Not only tumor sample availability is limited, but also the ability for tumor cells to rapidly acquire drug resistance are the rate-limiting bottlenecks for overall survival in current clinical settings. A liquid biopsy capable of capturing and enri...

Free access at https://rdcu.be/b9Etp. Ethical principles should prevail in the collection, testing and use of COVID-19 convalescent plasma (CCP) for human research in low-and middle-income countries. To appropriately guarantee safety, only blood establishments that comply with recognized quality standards should collect CCP. npj Vaccines (2020) 5:1...

https://authors.elsevier.com/a/1bg94WWN0mBpZ Nanotechnology and microfabrication approaches are playing instrumental roles in the development of innovative technologies to fight human diseases. Because of promising in vitro and preclinical outcomes, micro-/nanorobots (MNRs), are increasingly being considered for personalized and precision therapeut...

This Scientific Report 2020-2021 presents the main research activities carried out by the Professors and research teams of the College of Biomedical Engineering at Taipei Medical University, Taipei, Taiwan. The report highlights the main research focus of the College in the fields of (a) Biomedical Materials and Tissue Engineering, (b) Nanomedicine...

Interest in Africa on therapeutic use of COVID-19 convalescent plasma (CCP) has increased with continental spread of the pandemic of SARS -CoV-2. Consequently, blood organisations in several countries have established governmentally approved investigational protocols for collection and use of CCP. However, other countries still seek advice in this...

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Préambule: • Malgré des premiers rapports cliniques encourageants1, l'innocuité et l'efficacité de la transfusion de plasma de personnes guéries d’une infection à coronavirus SARS-CoV-2 (COVID-19) comme modalité thérapeutique n’ont pas encore été démontrées à ce jour. Cependant, les avantages théoriques de ce type de « plasma convalescent » et les...