Thierry Burnouf

Thierry Burnouf
Taipei Medical University | TMU · College of Biomedical Engineering

PhD

About

425
Publications
161,672
Reads
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Introduction
Distinguished Professor, Vice-Dean, Director of International PhD Program in Biomedical Engineering (IPBME), College of Biomedical Engineering (Dean: Prof Chih-Hwa Chen), Taipei Medical University. Fields include regenerative medicine, bioprocessing, cell therapy, platelet lysates, drug delivery, nanomedicine, medical devices, bio-optomechatronics and biosensors. Foreign students for MS and PhD degrees can contact me at thburnouf@gmail.com
Additional affiliations
November 2019 - present
Taipei Medical University
Position
  • Head of Faculty
August 2016 - present
Taipei Medical University
Position
  • Head of Faculty
August 2013 - present
Taipei Medical University
Position
  • Managing Director
Education
September 1977 - May 1981
Université de Lille
Field of study
  • PhD - Plant biochemistry and physiology

Publications

Publications (425)
Article
Microvesicles (MVs) are subcellular physiological vehicles present in all body fluids, mediate the transfer of intercellular information within biological systems, and contribute to healthy conditions. MVs have lipid bilayer membranes decorated with multiple ligands that can interact with receptors on target cells, rendering them promising for targ...
Article
There is much interest in the use of extracellular vesicles (EVs) as a subcellular therapy for regenerative medicine and drug delivery. Blood-borne platelets represent a source of therapeutic EVs that is so far largely unexplored. Advantages of platelets as a cellular source of EVs include their established clinical value, regulated collection proc...
Article
Free link to the PDF until Sept 5, 2017: https://authors.elsevier.com/a/1VOtFWWN0alX7 Human platelet lysates (PLs), which contain multiple neurotrophins, have been proposed for treating neurodegenerative disorders, including Parkinson’s disease (PD). However, current PLs suspended in plasma have high protein content and contain fibrinogen/fibrin...
Article
Full-text available
Background: Human platelets (PLT) and PLT-extracellular vesicles (PEV) released upon thrombin activation express receptors that interact with tumour cells and, thus, can serve as a delivery platform of anti-cancer agents. Drug-loaded nanoparticles coated with PLT membranes were demonstrated to have improved targeting efficiency to tumours, but rema...
Preprint
Full-text available
This preprint provides a commentary and further elaboration on the conclusions reached during a recent international workshop on plasma protein therapies organized by the Working Party for Global Safety of the International Society of Blood Transfusion (ISBT). The workshop addressed the profound deficiency in access to safe plasma protein therapies...
Article
Full-text available
Platelets, the small, anucleate blood cells that originate from megakaryocytes in the bone marrow, are typically associated with coagulation. However, it is now apparent that platelets are more multifaceted than originally thought, with their function extending beyond their traditional role in hemostasis to acting as important mediators of brain fu...
Article
Corneal endothelial cells (CECs) slowly decrease in number with increasing age, which is a clinical issue as these cells have very limited regenerative ability. Therapeutic platelet biomaterials are increasingly used in regenerative medicine and cell therapy because of their safety, cost-effective manufacture, and global availability from collected...
Article
Full-text available
Neurodegenerative disorders of the central nervous system (CNS) and brain traumatic insults are characterized by complex overlapping pathophysiological alterations encompassing neuroinflammation, alterations of synaptic functions, oxidative stress, and progressive neurodegeneration that eventually lead to irreversible motor and cognitive dysfunctio...
Article
The coronavirus disease 2019 (COVID-19) pandemic has highlighted the potential therapeutic value of early passive polyclonal immunotherapy using high-titer convalescent plasma (CCP). Human polyclonal hyperimmune immunoglobulin (HIG) has several advantages over CCP. Unlike CCP, HIG can provide standardized and controlled antibody content. It is also...
Article
The coronavirus disease 2019 (COVID-19) pandemic has highlighted the potential therapeutic value of early passive polyclonal immunotherapy using high-titer convalescent plasma (CCP). Human polyclonal hyperimmune immunoglobulin (HIG) has several advantages over CCP. Unlike CCP, HIG can provide standardized and controlled antibody content. It is also...
Article
The COVID-19 pandemic caused by the SARS-CoV-2 virus has significantly disrupted and burdened the diagnostic workup and delivery of care, including transfusion, to cancer patients across the globe. Furthermore, cancer patients suffering from solid tumors or hematologic malignancies were more prone to the infection and had higher morbidity and morta...
Article
Full-text available
Brain administration of human platelet lysates (HPL) is an emerging biotherapy of neurodegenerative and traumatic diseases of the central nervous system (CNS). HPLs being prepared from pooled platelet concentrates (PCs) increasing viral risks, manufacturing processes should incorporate robust virus‐reduction treatments. We evaluated a 19±2‐nm virus...
Article
Introduction Pancreatic ductal adenocarcinoma (PDAC) is highly aggressive and has poor prognosis. There are few biomarkers to inform treatment decisions, and collecting tumour samples for testing is challenging. Methods Circulating tumour cells (CTCs) from patients with PDAC liquid biopsies were expanded ex vivo to form CTC-derived organoid cultur...
Article
Platelets are at the crossroads between thrombosis and inflammation. When activated, platelets can shed bioactive extracellular vesicles [pEVs] that share the hemostatic potential of their parent cells and act as bioactive shuttles of their granular contents. In a viral infection, platelets are activated, and pEVs are generated with occasional viri...
Article
Full-text available
Background and Objectives: The coronavirus disease 2019 (COVID-19) pandemic has impacted blood systems worldwide. Challenges included maintaining blood supplies and initiating the collection and use of COVID-19 convalescent plasma (CCP). Sharing information on the challenges can help improve blood collection and utilization. Materials and Methods:...
Article
Full-text available
Convalescent plasma (CP) recurs as a frontline treatment in epidemics because it is available as soon as there are survivors. The COVID-19 pandemic represented the first large-scale opportunity to shed light on the mechanisms of action, safety, and efficacy of CP using modern evidence-based medicine approaches. Studies ranging from observational ca...
Article
Full-text available
Background and objectives: The coronavirus disease 2019 (COVID-19) pandemic has impacted blood systems worldwide. Challenges included maintaining blood supplies and initiating the collection and use of COVID-19 convalescent plasma (CCP). Sharing information on the challenges can help improve blood collection and utilization. Materials and methods...
Article
Background and objectives: Actions are needed to improve access to safe plasma-derived medicinal products (PDMPs) in low- and middle-income countries (LMICs). Materials and methods: The International Society of Blood Transfusion (ISBT) Working Party for Global Blood Safety organized an on-line workshop during 21-23 September 2021 to advance acce...
Article
Background and Objectives The coronavirus disease 2019 (COVID-19) pandemic has impacted blood systems worldwide. Challenges included maintaining blood supplies and initiating the collection and use of COVID-19 convalescent plasma (CCP). Sharing information on the challenges can help improve blood collection and utilization. Materials and Methods...
Article
Full-text available
Platelets have unique properties that can be used in a variety of clinical settings. Their ability to target and accumulate in the lung cancer microenvironment can be exploited as a therapeutic targeted delivery method. In this study, we developed a drug-free biomimetic platelet-polyethyleneimine polypyrrole (PLT-PEI-PPY) conjugate that expresses e...
Article
Full-text available
Published by Transfusion - https://onlinelibrary.wiley.com/share/author/SGRRT7AJSR7786NSCCJG?target=10.1111/trf.16810 Convalescent plasma (CP) has been used to treat emerging infectious diseases for over a century. It was notably deployed during the 1918 flu pandemic and has been explored during numerous disease outbreaks, including those caused by...
Article
There is concrete evidence that outdated allogeneic platelet concentrates not used for transfusion are valuable sources of functional trophic factors, including growth factors, cytokines, chemokines, anti-inflammatory and antioxidant molecules. Such outdated platelet concentrates can be used to produce human platelet lysates (HPL), a proven potent...
Article
Full-text available
The advanced-stage head and neck cancer (HNC) patients respond poorly to platinum-based treatments. Thus, a reliable pretreatment method for evaluating platinum treatment response would improve therapeutic efficiency and outcomes. This study describes a novel strategy to predict clinical drug responses in HNC patients by using eSelect, a lab-develo...
Article
Full-text available
Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease of motor neurons leading to death within 3 years and without a curative treatment. Neurotrophic growth factors (NTFs) are pivotal for cell survival. A reason for the lack of patient efficacy with single recombinant NTF brain infusion is likely to be due to the synergisti...
Article
Full-text available
Human platelet lysate (HPL) is a complex mixture of potent bioactive molecules instrumental in tissue repair and regeneration. Due to their remarkable safety, cost-effective production, and availability at global level from collected platelet concentrates, HPLs can become a powerful biotherapy for various therapeutic applications, if standardized a...
Preprint
Full-text available
Convalescent plasma (CP) recurs as a frontline treatment in epidemics because it is available as soon as there are survivors. The COVID-19 pandemic represented the first large-scale opportunity to shed light into mechanisms of action, safety and efficacy of convalescent plasma using modern evidence-based medicine approaches. Studies ranging from ob...
Preprint
Full-text available
Background Pancreatic ductal adenocarcinoma (PDAC) is highly aggressive and has poor prognosis. There are few biomarkers to inform treatment decisions, and collecting tumor samples for genomic or drug sensitivity testing is challenging.Methods Circulating tumor cells (CTCs) were prepared from the liquid biopsies of PDAC patients. These cells were s...
Article
Full-text available
COVID-19 convalescent plasma (CCP) is currently under investigation for both treatment and post-exposure prophylaxis. The active component of CCP mediating improved outcome is commonly reported as specific antibodies, particularly neutralizing antibodies, with clinical efficacy characterized according to the level or antibody affinity. In this revi...
Article
Full-text available
Background: Plasma-derived immunoglobulins (IgG) are essential medicines that are in worldwide shortage, especially in low- and middle-income countries. Optimised manufacturing processes can increase supply. We evaluated various new process steps for IgG fractionation. Material and methods: A crude, worst-case, IgG intermediate obtained by capry...
Article
Full-text available
Nanoparticle–Protein Corona Neural Interfaces In article number 2100694, Po-Chun Chen, Wei-Chen Huang, and co-workers develop a smart biotic-abiotic nano-assembly neural interface to provide controllable topographical, electrical, and chemical stimuli that can enhance neuronal activity. In the nanoparticle (NP)–protein corona structures, the soft N...
Article
Iridium oxide (IrOx) is a promising implantable electrode material owing to its remarkable neural stimulation capacity. However, presently, IrOx electrodes lack biocompatibility and bioactive interactions with nerve tissues. Application of polymeric surface coatings results in a weak physical adhesion at the organic/inorganic interface, which limit...
Article
Background aims Platelet concentrates (PCs) are pooled to prepare human platelet lysate (HPL) supplements of growth media to expand primary human cells for transplantation; this increases the risk of contamination by known, emerging, and unknown viruses. This possibility should be of concern because viral contamination of cell cultures is difficult...
Article
Full-text available
Traumatic brain injury leads to major brain anatomopathological damages underlined by neuroinflammation, oxidative stress and progressive neurodegeneration, ultimately leading to motor and cognitive deterioration. The multiple pathological events resulting from traumatic brain injury can be addressed not by a single therapeutic approach, but rather...
Article
Full-text available
Free access to the full text: https://onlinelibrary.wiley.com/doi/full/10.1111/vox.13114
Article
Background The lack of definitive treatment or preventative options for COVID‐19 led many clinicians early on to consider convalescent plasma (CCP) as potentially therapeutic. Regulators, blood centres and hospitals worldwide worked quickly to get CCP to the bedside. Although response was admirable, several areas have been identified to help impro...
Article
Full-text available
Vascular leiomyosarcoma is an extremely rare tumor and is associated with poor prognosis among leiomyosarcoma. Surgical resection remains the main treatment option. But outcome of definitive treatment with chemoradiotherapy in inoperable patients is not clear. Here, we report treatment and outcome of definitive chemoradiotherapy in a case of vascul...
Article
This article reviews how human platelet lysate can be used in place of FBS to expand human cells ex vivo: A pathogen-free and standardized xeno-free supplement of growth media is required for the ex vivo propagation of human cells used as advanced therapeutic medicinal products and for clinical translation in regenerative medicine and cell therapie...
Article
Human platelet lysates (HPLs), rich in various growth factors and cell growth-promoting molecules, encompass a new range of blood products that are being used for regenerative medicine, cell therapies, and tissue engineering. Well-characterized dedicated preparations, tailor-made to best fit specific therapeutic applications, are needed for optimal...
Article
Full-text available
Small cell lung cancer (SCLC) represents one of the most aggressive malignancies among cancer types. Not only tumor sample availability is limited, but also the ability for tumor cells to rapidly acquire drug resistance are the rate-limiting bottlenecks for overall survival in current clinical settings. A liquid biopsy capable of capturing and enri...
Article
Full-text available
Free access at https://rdcu.be/b9Etp. Ethical principles should prevail in the collection, testing and use of COVID-19 convalescent plasma (CCP) for human research in low-and middle-income countries. To appropriately guarantee safety, only blood establishments that comply with recognized quality standards should collect CCP. npj Vaccines (2020) 5:1...
Article
https://authors.elsevier.com/a/1bg94WWN0mBpZ Nanotechnology and microfabrication approaches are playing instrumental roles in the development of innovative technologies to fight human diseases. Because of promising in vitro and preclinical outcomes, micro-/nanorobots (MNRs), are increasingly being considered for personalized and precision therapeut...
Presentation
Full-text available
This Scientific Report 2020-2021 presents the main research activities carried out by the Professors and research teams of the College of Biomedical Engineering at Taipei Medical University, Taipei, Taiwan. The report highlights the main research focus of the College in the fields of (a) Biomedical Materials and Tissue Engineering, (b) Nanomedicine...
Article
Full-text available
Interest in Africa on therapeutic use of COVID-19 convalescent plasma (CCP) has increased with continental spread of the pandemic of SARS -CoV-2. Consequently, blood organisations in several countries have established governmentally approved investigational protocols for collection and use of CCP. However, other countries still seek advice in this...
Article
Full-text available
Préambule: • Malgré des premiers rapports cliniques encourageants1, l'innocuité et l'efficacité de la transfusion de plasma de personnes guéries d’une infection à coronavirus SARS-CoV-2 (COVID-19) comme modalité thérapeutique n’ont pas encore été démontrées à ce jour. Cependant, les avantages théoriques de ce type de « plasma convalescent » et les...
Conference Paper
Full-text available
LBS03.02- introduction: Custom-made Platelet Pellet Lysate (PPL) and Heat-treated PPL (HPPL) exert strong neuroprotective effects of neurotoxin-exposed dopaminergic LUHMES neuronal cell culture. This effect is significantly enhanced using HPPL, which was also highly protective of TH-expressing neurons in mice Parkinson’s disease (PD) model. The rol...
Article
Background and Objectives COVID‐19 convalescent plasma (CCP) has been used, predominantly in high‐income countries (HICs) to treat COVID‐19; available data suggest the safety and efficacy of use. We sought to develop guidance for procurement and use of CCP, particularly in low and middle‐income countries (LMICs) for which data are lacking. Materia...
Article
Most high‐income countries are aggressively engaged in the collection and research evaluation of convalescent plasma as a specific passive immunotherapy for treatment of COVID‐19. Preliminary clinical data fully support such initiatives. Outbreaks of COVID‐19 are now dramatically affecting LMIC, which have restricted capability for ensuring organiz...
Article
Background aims: Human platelet lysate can replace fetal bovine serum (FBS) for xeno-free ex vivo expansion of mesenchymal stromal cells (MSCs), but pooling of platelet concentrates (PCs) increases risks of pathogen transmission. We evaluated the feasibility of performing nanofiltration of platelet lysates and determined the impact on expansion of...
Technical Report
Full-text available
Document prepared by the Working Party on Global Blood Safety of the International Society of Blood Transfusion to assist low- and middle-income countries (LMIC) in the preparation of COVID-19 convalescent plasma as a potential treatment of COVID-19
Technical Report
Full-text available
This "Point to Consider" document has been prepared on behalf of the Working Party of Global Blood Safety of the International Society of Blood Transfusion (ISBT) to assist in the collection process and transfusion of plasma from patients who have fully recovered from COVID-19. This therapeutic approach relies on passive immunotherapy using neutral...
Article
This document prepared and endorsed by the Working Party on Global Blood Safety of the International Society of Blood Transfusion presents elements, as of April 2020, to take into consideration in the preparation and transfusion of COVID‐19 convalescent plasma as a possible treatment approach of COVID‐19. The document covers the following important...
Article
Full-text available
The neurorestorative efficacy of human platelet lysates in neurodegenerative disorders is still under investigation. Platelets prepared from standard and pathogen reduced platelet concentrates were pelletized, washed, concentrated, and subjected to freeze-thawing. The lysate was heated to 56°C for 30 min and characterized. Toxicity was evaluated us...
Article
https://www.cell.com/trends/biotechnology/fulltext/S0167-7799(19)30151-9. Human platelet lysate (HPL), rich in growth factors, is an efficient alternative supplement to fetal bovine serum for ex vivo propagation of stromal cell-based medicinal products. Since 2014, HPL is a focus of the Working Party for Cellular Therapies of the International Soc...