Thiago Barros-Galvão

John Innes Centre · Department of Crop Genetics

Light quality plays vital roles in the life cycle of plants. For example, in seeds of many species, light quality determines the levels of the gibberelins (GA) and abscisic acid (ABA) phytohormones, which promote and repress seed germination respectively (Seo et al., 2006). In Arabidopsis thaliana, the photoreceptors phytochrome A (PHYA) and PHYB d...

Light-dependent seed germination is induced by gibberellins (GA) and inhibited by abscisic acid (ABA). The widely accepted view of GA/ABA ratio controlling germination does not however explain the fact that seeds deficient in ABA still germinate poorly under shade light conditions that repress germination. In Arabidopsis, MOTHER-OF-FT-AND-TFL1 (MFT...

Seedling establishment is a critical step in environment colonization by higher plants that frequently occurs under adverse conditions. Thus, we carried out an integrated analysis of seedling growth, water status, ion accumulation, reserve mobilization, metabolite partitioning, and hydrolyse activity during seedling establishment of the native Caat...

In Arabidopsis, seed germination is a biphasic process involving rupture of the seed coat followed by emergence of the radicle through the micropylar endosperm. Embryo expansion results in seed coat rupture and removal of seed coat imposed dormancy with DELLA proteins blocking embryo expansion in the absence of gibberellins. Exogenous abscisic acid...

Seed germination in many plant species is triggered by sunlight, which is rich in the red (R) wavelength and repressed by under- the-canopy light rich in far red (FR). R:FR ratios are sensed by phytochromes to regulate levels of gibberellins (GAs) and abscisic acid (ABA), which induce and inhibit germination respectively. In this study we have disc...

We carried out in vitro feeding experiments using sunflower as a model to differentiate the modulatory effects of metabolites (sucrose and glutamine) and hormones (gibberellic acid and abscisic acid) on reserve mobilization, metabolite partitioning, and key enzyme activities. Exogenous sucrose negatively not only modulated the mobilization of carbo...

The aim of the current work is to review a protocol used in practical classes to demonstrate some factors that affect biomembrane integrity. Sugar-beet fragments were utilized as the experimental model as membrane damage could be visualized by leakage of betacyanins, hydrophilic pigments accumulated in the cell vacuoles. The tests were carried out...