Théo Aspert

Théo Aspert
Institut de Génétique et de Biologie Moléculaire et Cellulaire | IGBMC · Department of Biology and Stem Cell Development

PhD in Biophysics // Master of Bioengineering // M.S in Nanobiology //

About

10
Publications
10,354
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17
Citations
Introduction
I try to understand the mechanisms of replicative aging and its dynamics, with my favorite model organism: S. cerevisiae. To do this, I develop and use microfluidic and microscopy tools. Beside, I am also interested in the dynamics of entry into quiescence and response to starvation, using the same set of approaches.
Education
September 2016 - February 2017
Université Grenoble Alpes
Field of study
  • Nanosciences & Nanotechnologies
September 2014 - February 2017
Grenoble Institute of Technology
Field of study
  • Systems and microsystems for physics and biotechnologies

Publications

Publications (10)
Article
Full-text available
The nuclear pore complex (NPC) mediates nearly all exchanges between nucleus and cytoplasm, and in many species it changes composition as the organism ages. However, how these changes arise and whether they contribute themselves to ageing is poorly understood. We show that SAGA-dependent attachment of DNA circles to NPCs in replicatively ageing yea...
Article
Full-text available
The life cycle of microorganisms is associated with dynamic metabolic transitions and complex cellular responses. In yeast, how metabolic signals control the progressive choreography of structural reorganizations observed in quiescent cells during a natural life cycle remains unclear. We have developed an integrated microfluidic device to address t...
Preprint
Full-text available
Automating the extraction of meaningful temporal information from sequences of microscopy images represents a major challenge to characterize dynamical biological processes. Here, we have developed DetecDiv, a microfluidic-based image acquisition platform combined with deep learning-based software for high-throughput single-cell division tracking....
Preprint
Full-text available
Environmental oxidative stress threatens cellular integrity and should therefore be avoided by living organisms. Yet, relatively little is known about environmental oxidative stress perception. Here, using microfluidics, we show that the tail phasmid PHA neurons function as oxidative stress sensing neurons in C. elegans , and act in a complementary...
Preprint
Full-text available
The nuclear pore complex (NPC) mediates nearly all exchanges between nucleus and cytoplasm, and changes composition in many species as the organism ages. However, how these changes arise and whether they contribute themselves to aging is poorly understood. We show that in replicatively aging yeast cells attachment of DNA circles to NPCs drives the...
Preprint
Full-text available
The life cycle of microorganisms is associated with dynamic metabolic transitions and complex cellular responses. In yeast, how metabolic signals control the progressive establishment of structural reorganizations observed in quiescent cells remains unclear. To address this question, we have developed a method that combines nutrient-limited prolife...
Article
Full-text available
Single-cell analysis commonly requires the confinement of cell suspensions in an analysis chamber or the precise positioning of single cells in small channels. Hydrodynamic flow focusing has been broadly utilized to achieve stream confinement in microchannels for such applications. As imaging flow cytometry gains popularity, the need for imaging-co...

Questions

Questions (2)

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Projects

Projects (2)
Project
Developement of a microfluidic device to study replicative aging in Saccharomyces cerevisiae
Project
Studying how axonal transport translates neuronal activity into efficient targeting of synaptic and BDNF vesicles toward active synapses. Methods: Viral expression of fluorescent cargoes, superresolution videomicroscopy, multi array electrophysiology, 2-photon imaging, microfluidics, molecular and cellular biology, mathematical models