Tessa Moses

Tessa Moses
The University of Edinburgh | UoE · Centre for Synthetic and Systems Biology (SynthSys)

PhD

About

39
Publications
19,632
Reads
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1,918
Citations
Additional affiliations
June 2019 - present
The University of Edinburgh
Position
  • Metabolomics Specialist
Description
  • Running a metabolomics platform dedicated to metabolite analysis within the UK Centre for Mammalian Synthetic Biology.
June 2017 - May 2019
The University of Edinburgh
Position
  • PostDoc Position
Description
  • The project was performed in the research unit of Prof. Susan Rosser and was funded by the Industrial Biotechnology Innovation Centre (IBioIC). The project in collaboration with Unilever was entitled ‘Accelerating biotech production of saponins’.
October 2015 - May 2017
Ghent University
Position
  • Doctor Assistant with Research Assignment
Description
  • The project in the research unit of Prof. Alain Goossens was funded by the Special Research Fund (BOF) and entitled ‘Engineering yeast for the screening and sustainable production of high-value pharmaceutical and pesticidal triterpenoids’.

Publications

Publications (39)
Article
Full-text available
Metabolic rewiring is a critical hallmark of tumorigenesis and is essential for the development of cancer. Although many key features of metabolic alteration that are crucial for tumor cell survival, proliferation and progression have been identified, these are obtained from studies with established tumors and cancer cell lines. However, informatio...
Article
Full-text available
Abietane diterpenoids (ADs), synthesized in the roots of different Salvia species, such as aethiopinone, 1-oxoaethiopinone, salvipisone, and ferruginol, have a variety of known biological activities. We have shown that aethiopinone has promising cytotoxic activity against several human tumor cell lines, including the breast adenocarcinoma MCF7, HeL...
Article
Full-text available
Triterpenoids are high-value plant metabolites with numerous applications in medicine, agriculture, food, and home and personal care products. However, plants produce triterpenoids in low abundance, and their complex structures make their chemical synthesis prohibitively expensive and often impossible. As such, the yeast Saccharomyces cerevisiae ha...
Article
Full-text available
Yeast strains have been used extensively as robust microbial cell factories for the production of bulk and fine chemicals, including biofuels (bioethanol), complex pharmaceuticals (anti-malarial drug artemisinin and opioid pain-killers), flavours and fragrances (vanillin, nootkatone, resveratrol). In many cases it is of benefit to suppress or modif...
Article
Plants respond to herbivore or pathogen attacks by activating specific defense programs that include the production of bioactive specialized metabolites to eliminate or deter the attackers. Volatiles play an important role in the interaction of a plant with its environment. Through transcript profiling of jasmonate‐elicited Medicago truncatula cell...
Article
Full-text available
8,14-seco-triterpenoids are characterized by their unusual open C-ring. Their distribution in nature is rare and scattered in taxonomically unrelated plants. The 8,14-seco-triterpenoid α-onocerin is only known from the evolutionarily distant clubmoss genus Lycopodium and leguminous genus Ononis, which makes the biosynthesis of this seco-triterpenoi...
Article
Full-text available
The concept of growing plants for human health and general well-being, rather than for consumption as food alone, is changing people's perception of plant biotechnology and synthetic biology. Resurrecting the long-forgotten connection between plants and health has launched a new generation of botanical therapeutics, which include dietary supplement...
Article
Full-text available
Synthetic biology is the repurposing of biological systems for novel objectives and applications. Through the co-ordinated and balanced expression of genes, both native and those introduced from other organisms, resources within an industrial chassis can be siphoned for the commercial production of high-value commodities. This developing interdisci...
Article
Full-text available
Synthetic biology is the repurposing of biological systems for novel objectives and applications. Through the co-ordinated and balanced expression of genes, both native and those introduced from other organisms, resources within an industrial chassis can be siphoned for the commercial production of high-value commodities. This developing interdisci...
Article
Full-text available
Triterpenoids are widespread bioactive plant defence compounds with potential use as pharmaceuticals, pesticides and other high-value products. Enzymes belonging to the cytochrome P450 family have an essential role in creating the immense structural diversity of triterpenoids across the plant kingdom. However, for many triterpenoid oxidation reacti...
Data
CYP716 sequences used in the phylogenetic analysis of the CYP716 family sorted by subgroup. The columns indicate candidate name, resource, identifier, plant order, plant clade and identity to CYP716A12. Abbreviations: P450 HP, P450 homepage; PMS, PhytoMetaSyn website; CGP, The Compositae Genome Project website; L monilophytes, Leptosporangiate moni...
Data
Supplementary Figures 1-4, Supplementary Tables 1-6, Supplementary Methods and Supplementary References
Article
Full-text available
Differentiation of secondary metabolite profiles in closely related plant species provides clues for unravelling biosynthetic pathways and regulatory circuits, an area that is still underinvestigated. Cucurbitacins, a group of bitter and highly oxygenated tetracyclic triterpenes, are mainly produced by the plant family Cucurbitaceae. These compound...
Research
Full-text available
Conference on Plant Natural Products in Gent 21-24 November 2016 We are very pleased to announce that between the 21st and 24th of November 2016, we will be hosting the 'Plant Omics and Biotechnology for Human Health (POBHH)' Conference in Gent, within the framework of the Phytochemical Society of Europe. The PSE originated as the Plant Phenolic...
Article
Full-text available
Artemisia annua is widely studied for its ability to accumulate the antimalarial sesquiterpenoid artemisinin. In addition to producing a variety of sesquiterpenoids, A. annua also accumulates mono-, di-, and triterpenoids, the majority of which are produced in the glandular trichomes. A. annua also has filamentous trichomes on its aerial parts, but...
Article
Full-text available
Plants produce an array of specialized metabolites, including chemicals that are important as medicines, flavors, fragrances, pigments and insecticides. The vast majority of this metabolic diversity is untapped. Here we take a systematic approach toward dissecting genetic components of plant specialized metabolism. Focusing on the terpenes, the lar...
Article
Full-text available
Maesasaponins produced by the African shrub Maesa lanceolata are oleanane-type saponins with diverse biological activities. Candidate maesasaponin biosynthesis genes were identified through transcript profiling of M. lanceolata shoot cultures treated with methyl jasmonate, a well-known elicitor of plant specialized metabolism. Heterologous expressi...
Article
Full-text available
Abstract Saponins are widely distributed plant natural products with vast structural and functional diversity. They are typically composed of a hydrophobic aglycone, which is extensively decorated with functional groups prior to the addition of hydrophilic sugar moieties, to result in surface-active amphipathic compounds. The saponins are broadly c...
Article
Full-text available
Diatoms are unicellular photosynthetic microalgae that play a major role in global primary production and aquatic biogeochemical cycling. Endosymbiotic events and recurrent gene transfers uniquely shaped the genome of diatoms, which contains features from several domains of life. The biosynthesis pathways of sterols, essential compounds in all euka...
Article
Full-text available
The saikosaponins comprise oleanane- and ursane-type triterpene saponins that are abundantly present in the roots of the genus Bupleurum widely used in Asian traditional medicine. Here we identified a gene, designated CYP716Y1, encoding a cytochrome P450 monooxygenase from Bupleurum falcatum that catalyzes the C-16α hydroxylation of oleanane- and u...
Article
Full-text available
Jasmonates are ubiquitous oxylipin-derived phytohormones that are essential in the regulation of many development, growth and defence processes. Across the plant kingdom, jasmonates act as elicitors of the production of bioactive secondary metabolites that serve in defence against attackers. Knowledge of the conserved jasmonate perception and early...
Article
Full-text available
Terpenoids constitute a large and diverse class of natural products that serve many functions in nature. Most of the tens of thousands of the discovered terpenoids are synthesized by plants, where they function as primary metabolites involved in growth and development, or as secondary metabolites that optimize the interaction between the plant and...
Data
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Article
Full-text available
Background Recombinatorial cloning using the GatewayTM technology has been the method of choice for high-throughput omics projects, resulting in the availability of entire ORFeomes in GatewayTM compatible vectors. The MultiSite GatewayTM system allows combining multiple genetic fragments such as promoter, ORF and epitope tag in one single reaction....
Thesis
Plants are a rich source of natural products with diverse structures and biological activities beneficial to humans. This has led to their application as flavors and fragrances, colorants and therapeutics. Terpenoids or isoprenoids constitute the largest class of plant natural products with over 25,000 characterized structures. This dissertation fo...
Article
Full-text available
Combinatorial biochemistry, also called combinatorial biosynthesis, comprises a series of methods that establish novel enzyme-substrate combinations in vivo and, in turn, lead to the biosynthesis of new, natural product-derived compounds that can be used in drug discovery programs. Plants are an extremely rich source of bioactive natural products a...
Article
Full-text available
The phytohormones jasmonates (JAs) constitute an important class of elicitors for many plant secondary metabolic pathways. However, JAs do not act independently but operate in complex networks with crosstalk to several other phytohormonal signaling pathways. Here, crosstalk was detected between the JA and abscisic acid (ABA) signaling pathways in t...
Article
Full-text available
It is well-known that secondary metabolite production is repressed by excess nitrogen substrate available in the fermentation media. Although the nitrogen catabolite repression has been known, quantitative process models have not been reported to represent this phenomenon in complex medium. In this paper, we present a cybernetic model for rifamycin...

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