Terence Arthur Wilkins

University of Leeds · School of Chemical & Process Engineering

Poly (ethylene glycol) (PEG) is a widely used polymer in a variety of consumer products and in medicine. PEGylation refers to the conjugation of PEG to drugs or nanoparticles to increase circulation time and reduce unwanted host responses. PEG is viewed as being well-tolerated, but previous studies have identified anti-PEG antibodies and so-called...

Manufacturers of nanomaterial-enabled products need models of endpoints that are relevant to human safety to support the “safe by design” paradigm and avoid late-stage attrition. Increasingly, embryonic zebrafish ( Danio Rerio ) are recognised as a key human safety relevant in vivo test system. Hence, machine learning models were developed for iden...

INISS-nano “This concept paper shall prepare the ecosystem for global collaboration in selected fields of action (see pillars), enabling “collaboration without borders” within joint projects, joint funding initiatives, and any further way of cooperation.” Interested colleagues are invited to connect with the coordinators of the initiative. Furth...

The possibility of employing computational approaches like nano-QSAR or nano-read-across to predict nanomaterial hazard is attractive from both a financial, and most importantly, where in vivo tests are required, ethical perspective. In the present work, we have employed advanced Machine Learning techniques, including stacked model ensembles, to cr...

The convergence of nanotechnology and biotechnology has led to substantial advancements in nano-biomaterials (NBMs) used in medical devices (MD) and advanced therapy medicinal products (ATMP). However, there are concerns that applications of NBMs for medical diagnostics, Materials 2020, 13, 4532 2 of 29 therapeutics and regenerative medicine could...

A central challenge for the safe design of nanomaterials (NMs) is the inherent variability of NM properties, both as produced and as they interact with and evolve in, their surroundings. This has led to uncertainty in the literature regarding whether the biological and toxicological effects reported for NMs are related to specific NM properties the...

The innovation era has seen firms adopting a variety of organization designs with autonomous teams as their basic building blocks. Such organization designs have confronted firms with the challenge of managing complex task interdependence configurations. The predominant assumption within the organization design field for decades has been that task...

Despite the clear benefits that nanotechnology can bring to various sectors of industry, there are serious concerns about the potential health risks associated with engineered nanomaterials (ENMs), intensified by the limited understanding of what makes ENMs toxic and how to make them safe. As the use of ENMs for commercial purposes and the number o...

Nanoparticles (NPs) offer a number of advantages over small organic molecules for controlling protein behaviour inside the cell. Protein binding to the surface of NPs depends on their surface characteristics, composition and method of preparation (Mandal et al. in J Hazard Mater 248–249:238–245, 2013). It is important to understand the binding affi...

The number of engineered nanomaterials (ENMs) being exploited commercially is growing rapidly, due to the novel properties they exhibit. Clearly, it is important to understand and minimize any risks to health or the environment posed by the presence of ENMs. Data-driven models that decode the relationships between the biological activities of ENMs...

A successful electrodeposition method for preparing silver and zinc modified bioactive calcium phosphate layers onto surgical grade titanium alloy material (Ti6Al4V) was developed. The coatings were deposited on the Ti6Al4V surface by pulse current at 70 °C from an electrolyte containing adequate amounts of calcium nitrate, ammonium dihydrogen phos...

There is increasing recognition that some nanomaterials may pose a risk to human health and the environment. Moreover, the industrial use of the novel engineered nanomaterials (ENMs) increases at a higher rate than data generation for hazard assessment; consequently, many of them remain untested. The large number of nanomaterials and their variants...

Seemingly contradictory findings between studies are a major issue in nanoecotoxicological research and have been explained as a result of the lack of comparability between assay methods, with dispersion of nanomaterials being identified as a key factor. Here we show the use of a multivariate method, principal component analysis (PCA), as a tool in...

We determined binding characteristics of the triiodothyronine (T3) analog tracer used in the Amerlex and Amerlex-M FT3 radioimmunoassay for the three endogenous binding proteins in serum: thyroxin-binding globulin (TBG), thyroxin binding prealbumin (PA), and albumin. Both T3 and its analog bind to the same sites on TBG and PA. However, the analog h...

Abstract Regulation for nanomaterials is urgently needed, and the drive to adopt an intelligent testing strategy is evident. Such a strategy will not only provide economic benefits but will also reduce moral and ethical concerns arising from animal testing. For regulatory purposes, such an approach is promoted by REACH, particularly the use of quan...

During 2010-2011 the NMP Expert Advisory Group’s (EAG) established working groups to address 5 potential critical success factors for maximising the return on investment in the Key Enabling Technologies (KETs) programme in Horizon 2020 (2014-2020). The objective was to produce orientation papers to guide the European Commission and its KETs High-L...

Collaborative diversity is, arguably, an intrinsic characteristic of research networks built on the emergence of general-purpose technologies such as nanotechnology. European research policy, epitomised in Framework Programmes, creates arrangements that institutionalise the development of internationally and institutionally diverse research network...

TABLE OF CONTENTS 1. Terms of Reference 4 2. Europe 2020 -Innovation Union 10-Point Action Plan 5 3. Who Delivers Innovation? 6 4. Initial Scan of Best Practice 7 5. From Open Innovation to Co-Creation 8 5.1 Large industry model 9 5.2 Regional Industry-led “Open Innovation” Centre Model 10 5.3 Regional University-Industry “Open Innovation” Centre...

Crystallization of calcium oxalate monohydrate in a section of a single kidney nephron (distal convoluted tubule) is simulated using a model adapted from industrial crystallization. The nephron fluid dynamics is represented as a crystallizer/separator series with changing volume to allow for water removal along the tubule. The model integrates crys...

In the present report, the future research and technological directions of the NMP programme for the period (2010-2015) are discussed. In particular, the report addresses the following areas of research and technological priorities in relation to the NMP programme: • Nanoscience and Nanotechnology • Materials Science and Engineering • Industrial Pr...

We describe the first homogeneous, nonradioactive, high-sensitivity assay for human thyrotropin (TSH). The assay is based on particle immunoassay techniques, wherein 800-nm particles form the basis for the immunochemistry, delivery, and the detection technologies, respectively. Our assay also is the first to involve the use of fragmented monoclonal...

High-sensitivity homogeneous label-free nanoparticle counting immunoassay technique for a wide range fof clinical biomarkers in: endocrinology, haematology, neurology, oncology, obstetrics and gynaecology and infectious diseases. Fully automated flow injection and semi-automated microtitre plate methods are described,

We studied the effect of adding purified human albumin to sera on free thyroxine (FT4) values obtained with Amerlex radioimmunoassays. Apparent FT4 values increased with progressive addition of albumin in vitro. The effect was smallest with low and greatest with high initial FT4 concentrations, which were also linearly correlated with the increment...

An overview of the findings from the NATO Advanced Workshop on future road map for clinical applications of polymer colloids

We determined binding characteristics of the triiodothyronine (T3) analog tracer used in the Amerlex and Amerlex-M FT3 radioimmunoassay for the three endogenous binding proteins in serum: thyroxin-binding globulin (TBG), thyroxin binding prealbumin (PA), and albumin. Both T3 and its analog bind to the same sites on TBG and PA. However, the analog h...

Proceedings of 1985 W Berlin "Thyroid Symposium)

The basic theory of thyroxin-analog-based radioimmunoassays for free thyroxin has been extended to evaluate definitively the effects arising from residual binding of the tracer analog to serum proteins. Using experimentally determined binding constants and computer simulation techniques, we studied the effects of thyroxin-analog binding to serum pr...

Thyroid function in long term aspirin therapy.pdf

The interferences in FT4 assays reported by Boss et al. and Lundberg et al. are largely the result of uncontrolled conversion of plasma triglycerides in vitro by lipolytic activity stimulated in vivo by heparin. The question posed by Boss et al. as to which FT4 method gives the 'true' result invites the reply that no assay can give accurate FT4 est...

A new method is reported for the determination of the binding constants of homologous ligands relative to that of the parent ligand. The technique minimizes the effects of random and bias errors inherent in absolute methods and obviates the need to quantitate the radiolabelled tracer concentration. In addition, it is likely to be of general applica...

Serum free T4 concentrations have been variously reported as either constant or falling in pregnancy. In this study, 122 serum samples from apparently normal pregnancies were used to derive euthyroid ranges throughout pregnancy for T4 and free T 4 (measured by the Amerlex kits) and for T4-binding globulin (TBG). The mean free T4 concentration fell...

First scientific publication of a the generic 1-step free Thyroxine radioimmunoassay analog tracer principle describing the methodology, technical vailidation and first clinical results

This paper underpins recent publications on a generic approach for determining the concentrations of free analyte in the presence of a large percentage of protein bound analyte in serum, using the analogue tracer approach in radioimmunoassay. The assay is a single step method with no need to separate bound and free fractions before hand. This chapt...

A review of recently introduced 4 new commercial radioimmunossays for the direct measurement of (non-protein bound) of free thyroxine in human serum. It compared the performance of the 1-step analogue radiolabeled tracer method against 3 other methods using radiolabled thyroxine in 2 step methods. The 1-step method proved superior in diagnostic eff...

The compounds TiX(ox)2 (X=Cl or Br and oxH=quinolin-8-ol) and TiCl3.2oxH have been prepared from the reactions of quinolin-8-ol with α-TiCl3 and TiBr3 in acetonitrile. TiBr(ox)2 has also been prepared from the reaction of Ti(ox)3 and TiBr3 in acetonitrile.Reduction of TiCl(ox)3 by sodium in toluene yielded Ti(ox)3 and reaction of TiCl2(ox)2 with po...

Tris(quinolin-8-olato)titanium(III) has been prepared by reaction of α-titanium trichloride and quinolin-8-ol in acetonitrile. Magnetic-susceptibility measurements, e.s.r. and electronic absorption spectra, and X-ray powder diffraction studies indicate that the complex is a distorted octahedral monomer with an orbitally non-degenerate ground state.

DOI:https://doi.org/10.1103/PhysRev.47.251.3