Tej Khaket

Tej Khaket
Stanford University | SU · Department of Surgery

Ph.D

About

39
Publications
11,696
Reads
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661
Citations
Additional affiliations
July 2015 - November 2016
Maharishi Markandeshwar University, Mullana
Position
  • Professor (Assistant)
July 2019 - June 2021
The Ohio State University
Position
  • PostDoc Position
October 2016 - February 2019
Daegu University
Position
  • Professor

Publications

Publications (39)
Article
Myc is a major driver of tumor initiation, progression, and maintenance. Upregulation of Myc protein level rather than acquisition of neomorphic properties appears to underlie most Myc-driven cancers. Cellular mechanisms governing Myc expression remain incompletely defined. Here we show that ribosome-associated quality control (RQC) plays a critica...
Article
Full-text available
Aim: Oxidative stress and NAD+/NADH imbalance caused by alterations in reactive oxygen species (ROS) and NAD(H) metabolism are pathological features associated with normal aging and age-related diseases including Alzheimer’s disease (AD). How abnormalities in ROS and NAD(H) metabolism occur under these pathological conditions is not well understood...
Article
Full-text available
Anaplastic thyroid cancer (ATC) is the most aggressive thyroid cancer. Despite advances in tissue culture techniques, a robust model for ATC spheroid culture is yet to be developed. In this study, we created an efficient and cost-effective 3D tumor spheroids culture system from human ATC cells and existing cell lines that better mimic patient tumor...
Article
Loss of fragile X messenger ribonucleoprotein (FMRP) causes fragile X syndrome (FXS), the most prevalent form of inherited intellectual disability. Here, we show that FMRP interacts with the voltage-dependent anion channel (VDAC) to regulate the formation and function of endoplasmic reticulum (ER)-mitochondria contact sites (ERMCSs), structures tha...
Article
Full-text available
Mechanisms underlying the depletion of NAD+ and accumulation of reactive oxygen species (ROS) in aging and age-related disorders remain poorly defined. We show that reverse electron transfer (RET) at mitochondrial complex I, which causes increased ROS production and NAD+ to NADH conversion and thus lowered NAD+ /NADH ratio, is active during aging....
Article
Full-text available
An overarching goal of aging and age-related neurodegenerative disease research is to discover effective therapeutic strategies applicable to a broad spectrum of neurodegenerative diseases. Little is known about the extent to which targetable pathogenic mechanisms are shared among these seemingly diverse diseases. Translational control is critical...
Article
Full-text available
Oxya chinensis sinuosa (rice field grasshopper) is an edible insect with numerous health beneficial properties, traditionally being used to treat many ailments in Korea and other countries. O. chinensis sinuosa has been used from centuries, however, a little is known about the chemical functionality of its bioactive compounds. Therefore, this study...
Conference Paper
Ovarian cancer is the most frequent cause of death among gynecologic malignancies. Despite the availability of effective therapeutics against cancer, the overall 5-year survival rate of ovarian cancer is still ~30-40%. Most of the lethality are due to cancer relapse and drug resistance, where cancer stem cells (CSCs) are supposed to be the key regu...
Article
Full-text available
The cross-talk between apoptosis and autophagy influences anticancer drug sensitivity and cellular death in various cancer cell lines. However, the fundamental mechanisms behind this phenomenon are still unidentified. We demonstrated anti-cancerous role of cisplatin (CP) and morin hydrate (Mh) as an individual and/or in combination (CP-Mh) in hepat...
Article
Cathepsins are lysosomal acid hydrolases that make crucial contributions to tumor progression through a variety of signaling mechanisms, including autophagy, cell survival, chemotherapeutic resistance, and metastasis. Herein, we report that cathepsin C (CTSC) silencing upregulates the anticancer potential of curcumin in colorectal cancer cells (CRC...
Article
Cathepsins (CTS) are mainly lysosomal acid hydrolases extensively involved in the prognosis of different diseases, and having a distinct role in tumor progression by regulating cell proliferation, autophagy, angiogenesis, invasion, and metastasis. As all these processes conjunctively lead to cancer progression, their site-specific regulation might...
Article
The receptor for advanced glycation end products (RAGE) is a multi-ligand pattern recognition receptor that is highly expressed in lung epithelial cells. It helps alveolar epithelial cells to maintain their morphology and specific architecture. However, in various pathophysiological conditions, pulmonary tissues express supraphysiological level of...
Article
As Autophagy is a pivotal mechanism of cancer cell survival and the development of chemotherapeutic resistance; therefore, new approaches are warranted for its targeting which may be fulfilled by cathepsins regulation. Amongst cathepsins, cathepsin C (CTSC) is highly expressed in various cancers and possesses significant therapeutic potential in au...
Article
Carvacrol is a monoterpenoid flavonoid found abundantly in thyme plants. Its physiochemical instability and partial solubility in water is the principal limitation for its industrial use. Hence, we made a carvacrol nanoemulsion (CANE) using ultrasonication method and characterized it by dynamic light scattering (DLS) technique which revealed a nega...
Article
Full-text available
Cancer treatment is limited due to the diverse multidrug resistance acquired by cancer cells and the collateral damage caused to adjacent normal cells by chemotherapy. The flavonoid compound vitexin exhibits anti-oxidative, antiinflammatory and anti-tumor activity. This study elucidated the antitumor effects of vitexin and its underlying mechanisms...
Article
Background: Colon cancer is the second most common cancer to cause death worldwide. About half of colon cancer patients require adjuvant therapy to control relapse following surgical resection. Therefore, abolition of tumor cell progression using an effective chemotherapeutic agent holds a feasible approach to treat patients suffering from colon c...
Article
Background: Breast cancer is a notable cause of cancer-related death in women worldwide. Metastasis to distant organs is responsible for ~90% of this death. Breast cells convert to malignant cancer cells after acquiring the capacity of invasion/intravasation into surrounding tissues and, finally, extravasation/metastasis to distant organs (i.e., l...
Article
Synthetic inhibitors of Angiotensin Converting Enzyme (ACE) are commonly used in the treatment of hypertension and other cardiovascular diseases. But their diverse side effects stipulated nontoxic safer and economic inhibitors which can be accomplished by using inhibitors from natural sources or functional food ingradients. Dipeptidyl peptidases cl...
Article
Dipeptidyl peptidases (DPPs) are widely distributed exopeptidases that hydrolyse the dipeptide moieties from the N-termini of oligopeptide chains. In the present study, DPP-I was purified from germinated moong bean seeds via acid and ammonium sulphate precipitation followed by successive chromatographies, that is, gel filtration (pH 7.4), cation ex...
Article
Dipeptidyl peptidases (DPPs) are potent exopeptidases, which possess central role in proteolysis. As compared to other members of DPP family, proline containing dipeptide hydrolyzing activity of DPP-II (Dipeptidyl peptidase II) is unique as it hydrolyses imino group and plays a key role in protein metabolism. In present study, DPP-II was purified f...
Article
Tannase hydrolyzes tannins into glucose and gallic acid, both the abilities of tannins removal and production of gallic acid makes tannase an invaluable industrial enzyme with wide application in food, brewing, beverage and pharmaceutical industries. In present study, tannase was produced using Aspergillus awamori, and its immobilization studies we...
Article
Full-text available
Parthenium hysterophorus is an aggressive weed being invasive with allelopathic effect, it poses a serious threat to the environment and biodiversity. It adversely affects grazing animals and human beings, which directly or indirectly comes in contact with this weed. All ways to control it are not fully successful. However, recent reports suggest i...
Article
Full-text available
Parthenium hysterophorus is an aggressive weed being invasive with allelopathic effect, it poses a serious threat to the environment and biodiversity. It adversely affects grazing animals and human beings, which directly or indirectly comes in contact with this weed. All ways to control it are not fully successful. However, recent reports suggest i...
Article
Full-text available
Triticum vulgare (Wheat) based products are the major dietary source of food in developing countries. In India, it grows in association with boundary plantations of Populus deltoids (Poplar). During winter, poplar enters in dormancy which cause a heavy leaf fall at the time of wheat seed germination. Large number of poplar senescence leaves may adv...
Article
DING proteins are intriguing proteins characterized by conserved N-terminal sequence. Inspite of unusually high sequence conservation even between distantly related species, DING proteins exhibit outstanding functional diversity. An extracellular caseinolytic and phosphorolytic alkaline enzyme was purified to homogeneity from a probiotic lactic acid...
Chapter
Enzymes are large biological molecules responsible for thousands of metabolic processes that sustain life. They play a central role in the cellular metabolism of all the living organisms making enzyme research an intense area of activity. Enzymes have been extensively investigated for their action mechanisms, metabolic roles, structural, functional...
Chapter
Cancer is the leading cause of mortality worldwide. It is characterized by uncontrolled growth, invasion and metastasis of cells. There are different types of cancer with different causes of origin. Current cancer therapies have limited efficacy because of their known toxicity, ineffectiveness in specifically targeting or penetrating tumor tissue....
Article
Dipeptidyl peptidase-III (DPP-III) is an important enkephalin degrading enzyme and its inhibitors are expected to be promising in pain management. Some of its inhibitors showed an antinociceptive potential. The present study investigated the evaluation of dietary proteins as potential precursors of DPP-III inhibitors by measuring occurrence frequen...
Article
Full-text available
Chickpea (Cicer arietinum L.) is an ancient self-pollinated legume crop possibly originated in south-eastern Turkey and the adjoining part of Syria. The major objectives of chickpea breeding are to increase yield either by upgrading the genetic potential or by eliminating the effects of disease, pests and stress. Numerous selection techniques for p...
Article
Hepatitis B virus (HBV) is a hepatotropic DNA virus which belongs to the family Hepadnaviridae which includes viruses infecting birds and mammals. HBV remains an important cause of morbidity and mortality worldwide, especially in developing countries. HBV infection leads to a wide spectrum of liver conditions including acute self-limited infection,...
Article
Full-text available
Arginyl-arginyl-4-methoxy-β-naphthylamide (Arg-Arg-4mβNA) hydrolyzing activity is known as dipeptidylpeptidase-III (DPP-III). DPP-III enzyme hydrolyzes various bioactive peptides, including enkephalins, angiotensins, gastric peptides and Arg-Arg-4mβNA. We report the presence of DPP-III enzyme in plants for the first time. The enzyme was noticed in...
Article
Enkephalins play a great role in management of pain, blood pressure, hypertension and cardiovascular diseases. Enkephalins are short-lived molecules being rapidly hydrolyzed following their synaptic release by enkephalin degrading enzymes. The inhibitors of enkephalin degrading enzymes are able to prolong the duration of action of enkephalins. This...
Article
Genome analysis of plants indicated majority of putative protease genes that need characterization at enzymatic and molecular level. Proteases execute important role in seed development but knowledge of dipeptidylpeptidases in seeds is limited. A dipeptidylpeptidase-III that cleaves Arg-Arg from Arg-Arg-4mβNA was purified from germinated Mung bean...
Article
Full-text available
Hemoglobin is the iron-containing oxygen transporting metalloprotein in the red cells of blood in mammals and other animals. Hemoprotein-mediated oxidative stress is thought to play a major role in pathophysiology of cerebral hemorrhage, blast pressure injury, crush injury, myocardial ischemia reperfusion injury. Hemoglobin undergoes oxidation-redu...

Questions

Questions (2)
Question
Because single siRNA transfection results gene silencing upto 5-7 days, then is it possible to silent concerned gene in xenograft nude mice tumor model up to 15 days by multiple transfection (After 5 days) of shRNA (without vector) using transfection agent with direct intramural injection.
Question
I am planning to perform in vivo studies of a potent anticancer compound on HCT-116 cells based xenograft tumor model. I want to know the suitable treatment duration for the same.

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