How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
Citations since 2017
9 Research Items
Antithrombin (AT) deficiency is a rare but strong risk factor for the thrombosis development. Mutations in gene encoding AT (SERPINC1) have provided a detailed understanding of AT deficiency and subsequent development of thrombotic complications. In the present study, we describe a case of thrombotic patient with reduced AT activity and normal AT a...
Complement factor H (FH) and FH-related proteins (FHRs), structurally similar proteins are involved in the regulation of complement activation. Homozygous deletion of FHR 1 and 3 proteins (FHR1/3-/-) is known as a risk factor for disorders such as aHUS and SLE, characterised by thrombo-inflammatory complications. Interestingly, FHR1/3-/- genotype a...
A young Indian female visited hospital as a suspected case of thrombotic thrombocytopenic purpura (TTP) with relapsed thrombotic complications with low platelet counts, infarct in middle cerebral artery and thrombi in microvessels. We first confirmed the deficiency of ADAMTS13 metalloprotease in this patient showing improper cleavage of vWF multime...
In a recent work, we have described the kinetics among the monocyte subsets in the peripheral blood of hemolytic patients including paroxysmal nocturnal hemoglobinuria (PNH) and sickle cell disease (SCD). After engulfing Hb-activated platelets, classical monocytes (CD14 ⁺ CD16 ⁻ ) significantly transformed into highly inflammatory (CD14 ⁺ CD16 hi )...
List of proteins identified from the proteomic data from post-nuclear fraction of THP-1 cells treated with: A. only DV2, B. DV2-activated platelets or C. untreated platelets. MASCOT score was calculated from 2 biological replicates and 3 technical replicates.
A. List of proteins found to be involved in viral infection (VI), viral replication (VR) and platelet proteins (PP), analyzed using Ingenuity pathway analysis (IPA) with threshold p value < 3 × 10–15. Platelet proteins enlisted were also analyzed through the PlateletWeb software. B. List of 7 common proteins in the above 3 groups, along with relati...
Background Activated platelets release cytokines/proteins including CXCL4 (PF4), CCL5 and fibrinopeptides, which regulate infection of several pathogenic viruses such as HIV, H1N1 and HCV in human. Since platelet activation is the hallmark of Dengue virus (DV) infection, we investigated the role of platelets in DV replication and also in a closely...
Antithrombin III (AT) is the most important endogenous anticoagulant, and genetic variability in SERPINC1, gene encoding AT, is low. Mutations leading to AT deficiency and increased thrombotic risk are well known; however, only 2 studies have reported mutations in regulatory region of SERPINC1 gene till date. Aim of the present study was to identif...
Pro-coagulant, anti-coagulant and fibrinolytic pathways are responsible for maintaining haemostatic balance under physiological conditions. Any deviation from these pathways would result in hypercoagulability leading to life threatening diseases like myocardial infarction, stroke, portal vein thrombosis, deep vein thrombosis (DVT) and pulmonary emb...
Phenotypic resistance to activated protein C (APC) is a complex mechanism associated with increased thrombosis risk. Activated protein C resistance (APCR) is mainly influenced by FVLeiden mutation, and various other single nucleotide polymorphisms (SNPs) in FV gene are known to be associated with APCR. The aim of present study was to investigate th...
Antithrombin III (AT) is the main inhibitor of blood coagulation proteases like thrombin and factor Xa. In this study we report the identification and characterization of several variants of AT for the first time in Indian population. We screened 1950 deep vein thrombosis (DVT) patients for AT activity and antigen levels. DNA sequencing was further...
Recurrent pregnancy loss (RPL) can be caused due to diverse factors with thrombophilia being one of them. The association of various thrombophilic risk factors with RPL is inconsistent in different studies and the frequency of these risk factors in Indian population is obscure. Five hundred and eighty patients with either recurrent early miscarriag...
I need to quantify the pericyte area and pericyte coverage in mouse retina samples. Can anyone help me with the quantification steps on ImageJ? I plan to use NG-2 staining for pericytes and IB4 staining for endothelial cells.
Thank you in adavnce.
I want to quantify the cell area of bEnd.3 cells (mouse brain endothelial cells) using confocal microscopy by ImageJ. Most of the available protocols need to ignore neighbouring cells where cell-cell boundaries are in touch with each other. However, endothelial cells show continuous morphology. I will be really grateful if someone can help me with the protocol for cell area calculations using ImageJ.
I also want to quantify the distribution of tight junction proteins in cytoplasm vs. plasma membrane of these cells. If there is any way by which this quantification is possible using ImageJ then please help me.
Any help is appreciated.
Thank you in advance.
Hi...I want to do immunofluorescence staining for junctional proteins in bEnd.3 cells. Can anyone suggest me if I need to coat the cell culture plasticware with collagen? I am seeding cells on glass cover-slips in a 24 well plate. Literature suggests coating with type I collagen on transwell semipermeable supports for permeability assays but whether coating is required for immunofluorescence is not clear. I know collagen coating is required for other brain endothelial cells. But I am confused if I should coat every plasticware with collagen or not for bEnd.3 cells.
Any suggestions are appreciated.