tc Kuo

tc Kuo
Taipei Medical University | TMU

About

27
Publications
5,342
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250
Citations
Citations since 2016
8 Research Items
154 Citations
20162017201820192020202120220102030
20162017201820192020202120220102030
20162017201820192020202120220102030
20162017201820192020202120220102030

Publications

Publications (27)
Article
Full-text available
Functional mineral water and related products are popular in some Asian countries as health drinks and, recently, have been employed in agricultural crop production as well as pest control. This study aimed to investigate the survival of mosquito vectors exposed to plant-derived functional mineral water produced by terahertz technology. The teraher...
Article
To exploit the formation of DNA duplexes, the charge repulsion between complementary DNA strands can be reduced by adding salts or by replacing the negatively charged natural phosphodiester linkage(s) with charge-neutral methyl phosphotriester(s) (MPTE) linkage(s) of the synthetic nDNA oligonucleotides. Recently, we reported prominent improvements...
Article
Full-text available
Main conclusion The essential oils (EOs) of Plectranthus amboinicus showed the highest larvicidal activity among four herbal plants studied and β-caryophyllene might be the major component responsible for its differential toxicity to the larvae of Culex quinquefasciatus and Aedes Aegypti. Mosquitoes act as vectors for many life-threatening diseases...
Article
Full-text available
The world-to-chip interface is an essential yet intriguing part of making and employing microfluidic devices. A user-friendly connector could be expensive or difficult to make. We fabricated two ports of microfluidic chips with easily available materials including Teflon blocks, double adhesive films, coverslips, and transparency films. By using a...
Article
Full-text available
The roles of anisotropy of surface charge in the attraction (or repulsion) between molecules of same identity are probed by using myoglobin (Mb) and ribonuclease A (RNase A) as model proteins. To show the direction and extent of molecular interactions, we estimated second virial coefficients from the heat of protein dilution in isothermal titration...
Article
Isothermal titration calorimetry is widely used to measure the affinities and enthalpies of interaction between proteins and/or small molecules. The quantitative nature of the technique is especially useful in the characterization of recombinant proteins while determining the fraction of protein capable of binding a specific ligand and thus the pro...
Article
Efficient production is essential for developing baculoviruses into marketable bioinsecticides. In this study, we evaluated how juvenile hormone (JH) III and the JH analog pyriproxyfen affected the production efficiency of Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) Nucleopolyhedrovirus (SpltNPV) in SpltNPV-inoculated early sixth instars...
Article
Chitinases have an indispensable function in chitin metabolism and are well characterized in numerous insect species. Although the diamondback moth (DBM) Plutella xylostella , which has a high reproductive potential, short generation time, and characteristic adaptation to adverse environments, has become one of the most serious pests of cruciferous...
Article
Full-text available
The association constant of a well-known streptavidin–biotin binding has only been inferred from separately measured kinetic parameters. In a single experiment, we obtained Ka 1 × 1012 M−1 by using a streptavidin–binding aptamer and ligand-displacement isothermal titration calorimetry. This study explores the challenges of determining thermodynamic...
Article
Full-text available
Protein-nucleic acids binding driven by electrostatic interactions typically are characterized by the release of counter ions, and the salt-inhibited binding association constant (K(a) ) and the magnitude of exothermic binding enthalpy (ΔH). Here, we report a non-classical thermodynamics of streptavidin (SA)-aptamer binding in NaCl (140-350 mM) sol...
Article
Full-text available
Although fire ants frequently have negative impacts on agricultural systems and public health, they have additional beneficial insecticidal effects. To evaluate the potential effect of fire ant venoms on agricultural pests, the compositions of the venoms and their insecticidal activities against Plutella xylostella (L.) larvae were evaluated under...
Article
Kinesin motor proteins can hydrolyze ATP and unidirectionally transport specific cargo molecules along microtubule tracks (MTs). Engineering scientists interested in nanotechnology have used kinesin and MTs to build shuttles of 2 geometries. In the bead geometry, the kinesin shuttles transport cargos along the MTs. In the gliding geometry, cargos a...
Article
As shown in the literature, gold nanoparticles (NPs) were popularly used in the fields of catalyst and surface-enhanced Raman scattering (SERS). In this work, size-controllable Au NPs coated on TiO(2) are synthesized by adjusting the pH of solutions based on sonoelectrochemical methods. The size-controlled Au NPs on TiO(2), ranging from 2 to 80 nm...
Article
Full-text available
To better characterize the interaction of protein-cysteines with sodium arsenite, arsenic-binding proteins were identified from the arsenic-resistant Chinese hamster ovary cell line SA7 using a p-aminophenylarsine oxide (PAO)-agarose matrix in combination with proteomic techniques. Twenty of the isolated arsenic-binding proteins were further peptid...
Article
Full-text available
Water (H(2)O) is the most abundant and important molecule of life. Natural water contains small amount of heavy isotopes. Previously, few animal model studies have shown that the isotopic composition of body water could play important roles in physiology and pathophysiology. Here we study the stable isotopic ratios of hydrogen (δ(2)H) and oxygen (δ...
Article
A pre-cryogenic holder (cryo-holder) facilitating cryo-specimen observation under a conventional scanning electron microscope (SEM) is described. This cryo-holder includes a specimen-holding unit (the stub) and a cryogenic energy-storing unit (a composite of three cylinders assembled with a screw). After cooling, the cryo-holder can continue supply...
Article
The development of protein chips has suffered from problems regarding long-term protein stability and activity. We present a protein sensor surface for immunodetection that is prepared by a DNA-directed protein immobilization method on a mixed self-assembled monolayer (SAM). By this approach, an immobilized single-stranded DNA (ssDNA) surface can b...
Article
Galectin-1 (GAL1) is known as a β-galactoside-binding protein that also can bind with arsenic to regulate cell functions. Using RNA interference technique, we investigated the possible mechanism involved in GAL1 modulation of arsenite-inhibited cell survival in 3T3 fibroblast and KB oral cancer cells. GAL1 gene knockdown significantly attenuated so...
Article
Full-text available
To solve the problems of measuring the growth rates of microorganisms from optical density (OD)-growth time plots, we used relative-density (RD) plots. The relationship of OD and RD was built from the diluted grown cultures. This method was satisfactorily applied to study the growth of Escherichia coli and the cyanobacterium Anabaena spiroides.
Article
Full-text available
We have developed a novel class of antisense agents, RNA Lassos, which are capable of binding to and circularizing around complementary target RNAs. The RNA Lasso consists of a fixed sequence derived from the hairpin ribozyme and an antisense segment whose size and sequence can be varied to base pair with accessible sites in the target RNA. The rib...
Article
Systematic evolution of ligands by exponential enrichment (SELEX) is an in vitro combinatorial engineering approach to enrich aptamers from a library of nucleic acids ligands by iterative extraction and amplification of receptor-bound ligands. Aptamers are the selected nucleic acid ligands with high receptor-binding affinity. Typically, they are ob...
Article
Full-text available
Group I intron ribozymes require cations for folding and catalysis, and the current literature indicates that a number of cations can promote folding, but only Mg2+ and Mn2+ support both processes. However, some group I introns are active only with Mg2+, e.g. three of the five group I introns in Chlamydomonas reinhardtii. We have investigated one o...
Article
Full-text available
Manganese (Mn2+) promotes specific cleavage at two major (I and III) and four minor (II, IV, V and VI) sites, in addition to slow non-specific cleavage, in a 659-nucleotide RNA containing the Cr.LSU group I intron. The specific cleavages occurred between G and AAA sequences and thus can be considered Mn2+-GAAA ribozymes. We have estimated rates of...
Article
Full-text available
The 23S rRNA gene of Chlamydomonas reinhardtii contains a group IA3 intron, Cr.LSU, whose splicing is essential for cell growth. To better understand Cr.LSU splicing, kinetic analyses were undertaken with 23S.3, a preRNA previously shown to self-splice. Self-splicing of 23S.3 showed biphasic kinetics, with only approximately 33% reacting efficientl...
Chapter
The chloroplast genomes of Chlamydomonas spp. contain introns that belong to the two major classes found in organelles: group I and group II. Some of the members of both classes are ribozymes capable of self-splicing in vitro and, indeed, most of the group I introns studied in Chlamydomonas spp. are autocatalyic. The biochemical characterization of...

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