Tatsuya Nakayama

Tatsuya Nakayama
Hiroshima University | HU · Graduate School of Integrated Sciences for Life

About

46
Publications
4,304
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1,012
Citations
Citations since 2017
24 Research Items
693 Citations
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2017201820192020202120222023020406080100120140
2017201820192020202120222023020406080100120140
2017201820192020202120222023020406080100120140

Publications

Publications (46)
Article
The threat of antimicrobial resistance is increasing. Microbial food contamination poses a serious public health risk; however, there are only a few studies on the prevalence of colistin-resistant Escherichia coli (COL-E) contamination in freshwater fish. This study aimed to characterise the antibiotic resistance genes and antibiotic susceptibility...
Article
Gentamicin is an important antibiotic for the treatment of opportunistic infections in the clinical field. Gentamicin-resistant bacteria have been detected in livestock animals and can be transmitted to humans through the food supply or direct contact. We have previously revealed that gentamicin-resistant Escherichia coli are distributed at a compa...
Article
Full-text available
A carbapenem-resistant Enterobacter cloacae 0102-4P-1 strain was isolated from commercially imported shrimp in Japan. Here, we present a draft genome sequence. The complete plasmid sequence was also determined by hybrid assembly sequencing using Oxford Nanopore and Illumina methods. The assembled whole genome and plasmid were 5,164,033 bp and 162,8...
Article
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Although the spread of plasmid-mediated antibiotic-resistant bacteria is a public health concern, food contamination with plasmid-mediated antibiotic-resistant Escherichia coli in Vietnam has not been well investigated. This study aimed to describe the prevalence of colistin-resistant, carbapenem-resistant, and endemic blaCTX-M in extended-spectrum...
Article
The spread of potentially life-threatening carbapenem-resistant bacteria is a major threat to public health. This study aimed to determine the prevalence of carbapenem-resistant Enterobacteriaceae in fishery products in Vietnam. A total of 103 river fish were purchased from retail outlets and supermarkets in Vietnam. The gut contents of fish were r...
Article
The pathology of streptococcal meningitis is poorly understood, even though streptococcal infection induces meningitis. The aim of this study was to clarify the relationship between streptococcal meningitis and aquaporin 4 (AQP4) in the mouse brain. After Streptococcus suis infection, the streptococcal number was calculated, and AQP4 mRNA expressio...
Preprint
Full-text available
Although the spread of plasmid-mediated antibiotic-resistant bacteria is a public health concern, food contamination with plasmid-mediated antibiotic-resistant Escherichia coli has not been well investigated in Vietnam. The aim of this study was to describe the prevalence of colistin-resistant, carbapenem-resistant and endemic bla CTX−M in extended...
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The prevalence of food-borne bacteria in developing countries is less well understood than in developed countries. The ISO11290-1 isolation method is commonly used to study Listeria contamination in chicken; however, all isolates are identified as untargeted Bacillus cereus. This study aimed to determine the classification, antibiotic susceptibilit...
Article
Food contamination with extended-spectrum beta-lactamase (ESBL)-producing bacteria is recognized as one of the public health concerns worldwide. Although fish and fishery products at retail in Japan have relied on the imported materials, no apparent evidences exist on the prevalence and property of genetic transfer in the ESBL-producing bacteria in...
Article
Full-text available
Campylobacter jejuni is one of the leading causes of gastrointestinal illness worldwide and is mainly transmitted from chicken through the food chain. Previous studies have provided increasing evidence that this pathogen can colonize and replicate in broiler chicken during its breeding; however, its spatiotemporal kinetics in laying hen are poorly...
Preprint
Full-text available
The pathology of streptococcal meningitis is poorly understood, even though streptococcal infection induces meningitis. The aim of this study was to clarify the relationship between streptococcal meningitis and aquaporin 4 (AQP4) in the mouse brain. After Streptococcus suis infection, the streptococcal number was calculated, and AQP4 mRNA expressio...
Article
Full-text available
Stenotrophomonas maltophilia is a nosocomial pathogen that primarily causes respiratory infection in humans. This pathogen is widely distributed in the environment, including in foods. Here, we report the draft genome sequence of S. maltophilia strain CRB139-1, isolated from poultry meat in Japan. The genome size was 4,619,918 bp at 90× coverage.
Article
Full-text available
Objectives: Extended-spectrum β-lactamase and AmpC β-lactamase-producingEscherichia coli (ESBL/AmpC-E. coli) are common in developing countries, and travelers represent potential reservoirs for their dissemination. Our aim was to quantify cefotaxime (CTX)-resistant E. coli harbored by travelers to Vietnam, and to conduct a follow-up study. Method...
Article
This study investigated the existence of sulfonamides and colistin resistance genes among extended‐spectrum beta‐lactamase (ESBL)‐producing E. coli recovered from fish gut in Vietnam and evaluated the susceptibility patterns of the ESBL‐producing E. coli to relevant antimicrobials. A total of 88 ESBL‐producing E. coli isolates were analyzed for the...
Article
Full-text available
The original version of this article unfortunately contained a mistake. The legends of Tables 2 and 3, Fig. 1 are incorrect. The corrected legends are given below.
Article
Colistin is indicated for the treatment of multidrug-resistant gram-negative bacterial infections. However, the spread of colistin-resistant bacteria harboring a mcr gene has become a serious concern. This study investigated local foods in Vietnam for contamination with colistin-resistant bacteria. A total of 261 extended-spectrum β-lactamase (ESBL...
Article
Full-text available
Background Due to increasing colistin usage, the dissemination of the colistin-resistant gene mcr-1 has been increasingly investigated. The aim of this study was to determine whether a traveler on a short-term international trip to a developing country could bring mcr-1 back to their home country. Materials and methods Thirty-four travel events fr...
Article
Extended-spectrum β-lactamase-producing Escherichia coli (ESBL-E) are becoming increasingly widespread in Vietnam. Antibiotics are detected in many Vietnamese foods; however, the effect of ESBL-E and antibiotic consumption on intestinal bacteria has not been studied sufficiently. Here, we investigated the effect of oral administration of ESBL-E (TB...
Article
Although Vietnamese residents frequently harbor extended-spectrum β-lactamase-producing Escherichia coli (ESBL-E), it is unclear which foods/beverages are risk factors for acquiring these bacteria. The aim of this study was to evaluate the frequency with which edible ice served in restaurants is contaminated with antibiotic-resistant bacteria, and...
Article
Streptococcus suis is an important zoonotic pathogen in swine and humans that causes sepsis and meningitis. Our previous study in Thailand showed that the prevalence of S. suis infection in humans, especially in northern areas of Thailand, and the transmission of the pathogen occurred mainly through the consumption of traditional raw pork products....
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Full-text available
Extended-spectrum, β-lactamase-producing Escherichia coli (ESBL-E) harboring the blaCTX-M-55-encoding plasmid (ESBL-E55) has been reported to be associated with urinary tract infection (UTI). The aims of this study were to clarify the prevalence of ESBL-E55 in pork meats and workers from the same wholesale market, as well as patients with UTI from...
Article
The environmental pathways for the dissemination of antibiotic resistance have recently received increased attention. Aquatic environments act as reservoirs or sources of antimicrobial-resistant bacteria, antimicrobial residues, and antimicrobial resistance genes (ARGs). Therefore, it is imperative to identify the role of polluted water in the diss...
Article
Full-text available
Reports of livestock infections with extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-E) are increasing. Based on interviews conducted over a 6-month period, we found that veterinarians in the Vietnamese province of Thai Binh prefer to prescribe colistin-based drugs (CBD) in chicken farms. We aimed to clarify whether CBD use select...
Article
Full-text available
Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli were isolated from wild and cultured fish (18 striped catfish, Pangasianodon hypophthalmus, and 18 red tilapia, Oreochromis sp.) in the Mekong Delta, Vietnam, from June 2014 to October 2014. In total, 39% of the fish harbored ESBL-producing E. coli, including 44.4% of wild fish and 36....
Article
Full-text available
Background Widespread dissemination of extended-spectrum β-lactamase (ESBL)-producing bacteria is a threat to public health. Since livestock products are possible reservoirs of ESBL-producing bacteria, food-borne dissemination of ESBL-producing bacteria and the characteristics of such organisms in food products should be assessed to evaluate potent...
Article
Contamination of food with multiantibiotic-resistant bacteria, particularly extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae, is considered a potential source for the wide dissemination of ESBL-producing bacteria in communities. However, little is known about the extent of contamination of food with ESBL-producing bacteria in Vietn...
Article
Full-text available
Recent studies have reported a widespread distribution of extended-spectrum β-lactamase (ESBL)-producing bacteria, not only in the nosocomial setting, but also in the community; some local communities in Southeast Asia have been reported to show a high prevalence of ESBL-producing bacteria. However, the details regarding the quantitative/qualitativ...
Article
Streptococcus species release cholesterol-dependent cytolysins (CDCs), which are a main toxin, and their heat susceptibility is poorly understood. The aim of this study was to clarify the heat susceptibility of streptococcal exotoxins and CDCs. Streptococcal exotoxins were treated with heat incubation at 60 °C for 10 or 30 min. The Streptococcus su...
Article
Full-text available
Background: Streptococcus suis is an emerging zoonotic pathogen, and causes sepsis and meningitis in humans. Although sequence type (ST) 1 and ST104 strains are capable of causing sepsis, ST1 strains commonly cause meningitis. In this study, we investigated the role of suilysin, a member of cholesterol-dependent cytolysins, in differential pathoge...
Article
Streptococcus suis is an emerging zoonotic agent. This study aimed to investigate whether S. suis is likely to translocate across the intestines of human hosts who have liver disease and/or consume alcohol. Both the alcoholism and cirrhosis models exhibited high mRNA expression of TGF and collagen1, but only the cirrhosis model had fibrosis in the...
Article
Although studies have reported numerous effects of coffee on human health, few studies have examined its specific effects on gut microbiota. This study aimed to clarify the influence of coffee and galacto-oligosaccharide (GOS) consumption on gut microbiota and host responses. After mice consumed coffee and GOS, their intestines were sampled, and th...
Article
Full-text available
Streptococcus suis infection in humans has received increasing worldwide recognition. A prospective study of S. suis infection in humans was conducted in Phayao Province in northern Thailand to determine the incidence and the risk behaviors of the disease in this region in 2010. Thirty-one cases were confirmed. The case fatality rate was 16.1%, and...
Article
Full-text available
To examine associations between clinical features of Streptococcus suis serotype 2 infections in humans in Thailand and genotypic profiles of isolates, we conducted a retrospective study during 2006-2008. Of 165 patients for whom bacterial cultures of blood, cerebrospinal fluid, or both were positive for S. suis serotype 2, the major multilocus seq...
Article
Although a Streptococcus suis infection was recently expanded, the study of S. suis has not had enough attention. In this study, bacterial kinetics of S. suis were investigated for seven days of infection in a mouse model. Survival rates showed that 5.0 × 10(7) CFU ml(-1) of S. suis infection caused death in 30% of mice subjects. Streptococcal kine...
Article
To investigate the effects of Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium in terms of toxin and growth of pathogenic Vibrio harveyi. Three Bacillus probionts were isolated from probiotic BZT aquaculture and identified using a 16S rDNA sequence. Growth inhibition assay showed that supernatants from the 24-h culture of three Bac...
Article
Full-text available
Crohn's disease and ulcerative colitis are the two principal forms of inflammatory bowel disease (IBD). The root causes of these chronic and acute immunological disorders are unclear, but intestinal microorganisms are known to play a key role in the initiation and maintenance of disease. However, at present, there is no clear evidence for a single...
Article
To demonstrate the influence of copper on luminescence and toxin production in Vibrio harveyi. The effect of copper concentration on the expression of both luminescence and toxin of V. harveyi was investigated. Copper concentration of less than 40 ppm had no effect on the growth. While V. harveyi cultured with 40 ppm copper concentration showed dec...
Article
To demonstrate that Vibrio harveyi produces various types of toxins and how the production of those toxins is related with luminescence. Luminescence and toxicity of eight V. harveyi were evaluated. We demonstrated that all V. harveyi emitting luminescence were isolated from marine organisms and also showed that they were highly pathogenic when com...
Article
Vibrio harveyi strains isolated from shrimp farms (wild strains) were compared with those from culture collections in terms of minimum inhibitory concentration (MIC) and toxicity. Wild strains had higher MIC values for four antibiotics (kanamycin, carbenicillin, oxytetracycline and ampicillin) and also showed higher toxicity compared with culture c...
Article
Aquaculture deterioration is caused by Vibrio harveyi, which is one of the main shrimp pathogens. Vibrio carchariae having the same phenotypic characteristics as V. harveyi was isolated from a shrimp farm and used in this study to investigate the relationship between luminescence and toxicity. Luminescence was verified by monitoring the expression...

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Projects (2)
Project
This study focuses on three foods of chicken meats, fishery products and vegetables. We plan to isolate Campylobacter, Salmonella and Listeria from chicken meats, Vibrio, Salmonella and Listeria from fishery products, and Salmonella and Listeria from vegetables. ESBL-E and colistin resistant E. coli are also isolated from all foods samples. All isolated bacteria are clarified characteristics and antibiotic susceptibility. Moreover, for clarification of horizontal gene transfer, plasmid analysis is conducted by next generation sequencer.