Tatsadjieu Ngoune Léopold

University of Ngaoundere · Department of Food Engineering and Quality Control

Defatted Lagenaria siceraria seed flour (DLSSF) was obtained from defatted seed cake, dried, and ground through a sieve of 500 μm and characterized. A 2 × 4 factorial design (two flour hydration rates and four fat substitution rates) was used to produce a low‐fat beef patty by replacing fat with DLSSF. Beef kidney fat was used to formulate the cont...

The microorganisms intended for use as probiotics in food formulation should exert health benefit effects and be regarded as safe for animals and humans uses. The aim of this study was to evaluate the probiotic potential of lactic acid bacteria (LAB) isolated from pendidam and kindirmou , two traditional fermented milks (TFM) produced in the Adamaw...

Kargasok" is an artisanal drink with a tangy, slightly sparkling flavor obtained by fermenting sweet tea (green or black tea). This drink is highly prized for its therapeutic properties and as a means of subsistence for the producers, but its production and quality are unknown. A study was then undertaken to highlight the manufacturing process and...

The Chechim nchabe is a traditional food widely consumed in Foumban, Foumbot, Koutaba, Massangam, Kouoptamo, Malentouen and Magba, 07 Departments of Noun (West region of Cameroon). It is obtained by fermenting cassava sticks cooked on the surface of river or spring water. Unfortunately, the bad hygienic quality of the environment during production...

This study aims to evaluate the effect of Afrostyrax lepidophyllus spice on microbiological, chemical and sensory stability of hot smoked fillets of Pangasius hypophthalmus. Treatments included control (hot smoked fish without spice) and hot smoked pre-treated fillets with 10% spice powder of A. lepidophyllus. The hot smoked fillets of P. hypophtha...

This study aimed to evaluate the influence of fermentation time on the quality attributes and shelf life of the date beverage from the North of Chad. The must from date fruits was prepared and fermentation was initiated using Saccharomyces cerevisiae (1:25 v/v) as starter for a week. Physicochemical, microbiological and sensory parameters were eval...

The objective of this study was to evaluate the shelf life of low-fat beef patty formulated using defatted Cucurbita maxima Duchesne seed flour (DCM x SF) paste as fat replacers during cold storage. The samples formulated using DCM x SF paste containing 60% of water to replace fat at 75(CMx360) and 100 % (CMx460) as well as the control processed us...

Defatted Lagenaria siceraria seeds and those of Cucumeropsis mannii, obtained after lipid extraction using food-grade hexane, were converted into flours (defatted Lagenaria siceraria seeds flours, LSDSF; defatted Cucumeropsis mannii seed flours, CMDSF), and analysed for their chemical and amino acid (AA) compositions, protein fractions, protein mol...

This study aimed to evaluate the quality and antimicrobial activity of soaps and ointments enriched with aqueous ethanol extracts from barks of Ziziphus mauritiana and Ziziphus mucronata. The galenic formulation allowed the development of ointments as well as soap based on aqueous ethanol extract (70%, v/v) using some excipients. The consistency of...

Rice is an important cereal for Cameroon where 95% of the population consumes it frequently. In order to ensuring a healthy food supply to consumers, the objective of this study was to determine the fungal profile and quantify mycotoxins in twelve rice samples produced in Cameroon. The relative density of the different fungal species identified in...

The resistance of Bacillus cereus (B. cereus) cells to fermentation and drying in 4 different dough based on cereal flours (malted and unmalted sorghum, malted and unmalted maize) was studied. B. cereus vegetative cells in order of 107 cfu g 1 inoculated in the dough before the onset of fermentation increased to 108 cfu g 1 after fermentation for 4...

Les substrats amylacés sont les plus abondants après la cellulose, ils constituent des sources importantes de matières premières valorisables, en particulier dans la production d'éthanol. Les levures de distillerie communément utilisées étant dépourvues de l'activité amylasique, la production d'éthanol à partir de l'amidon nécessite un prétraitemen...

Background and Objective: In the northern part of Chad, dried dates are used in the production of a traditional drink highly cherished by the popuation. Despite the importance of this beverage, the processing, microbiological and physicochemical qualities remain unknown. To fill these gaps, this study aimed to describe the main stages of the manufa...

The objective of this study was to evaluate the shelf life of low-fat beef patty formulated using defatted Cucurbita maxima Duchesne seed flour (DCMxSF) paste as fat replacers during cold storage. The samples formulated using DCMxSF paste containing 60 % of water to replace fat at 75 (CMx360) and 100 % (CMx460) as well as the control processed usin...

The objective of this study was to determine the phytochemical profile and antimicrobial activity of leaf and bark extracts of Ziziphus mauritiana and Ziziphus mucronata. For this purpose, an extraction by maceration using ethanol was carried out and the extracts were subjected to antibacterial activity assessment through qualitative and quantitati...

Background and Aim: Lactic acid bacteria (LAB) became a field of interest by scientists in recent years due to their technological and probiotic properties. The aim of this work was to study the technological and probiotic properties of LAB isolated from the bottle gourds (calabashes)of milk fermentation, in Mbéré, Cameroun. Methods: Five different...

Recent data show that the containers traditionally used for fermentation, due to their nature, exchange with the fermented product, the consequence being a modification of the physico-chemical, microbiological and organoleptic properties of these products. The objective of this work is to study the influence of the fermentation vessel on the sensor...

The objective of this study was to identify the dominant bacterial flora linked to the hygiene of dried and smoked fish in different geographical locations and to determine the presence of pesticide residues on the processed fish. Fish species were listed and collected. The bacterial ecology of dried and smoked fish from the northern zone of Camero...

In order to provide an effective alternative for efficient management of dermatophytosis, we evaluate in vitro the antioxidant and antiradical potentials of the essential oil of the dry flower buds of Syzygium aromaticum and its antidermatophytic activity against Epidermophyton floccosum and Trichophyton soudanense, For this purpose, the essential...

The present study consists of analyzing the phytochemical composition of Ipomoea batatas leaf powders and evaluating their antihyperlipidemic effect on rats receiving a high-fat diet. Ipomoea batatas leaves were collected from four agroecological areas of Cameroon, and powders were obtained after washing, drying, grinding, and sieving. Standard ana...

The present study had as objective to assess the microbiological quality and profile of some braised products and their accompanying soups sold in the city of Bangangte (Cameroon) and evaluate the antibiotic susceptibility of selected pathogens isolated in these products. An investigation was conducted in three locations in city of Bangangte in ord...

This study aims at characterizing new chicken feather-degrading bacteria isolated from soils of keratinic waste collected from four dumping sites of Ngaoundere. Fifteen proteolytic bacteria were isolated with three (D1, D2 and F1) showing the capacity to degrade feather in Basal Salt Media. Highest degradation rate (71.11 ± 3.05%) of feather was ob...

Probiotics are known for their beneficial properties. Numerous studies have been conducted to find advantages that probiotics can provide. This study aimed to evaluate the functional properties of raffia sap, a Cameroonian drink, fermented with probiotics by investigating its antagonistic activity against pathogenic bacteria. Box-Behnken design wit...

The present study is aimed at producing Sorghum bicolor and Voandzeia subterranea complementary instant flour. The precooked sorghum and Bambara groundnut flours were mixed at different proportions (0 : 100, 25 : 75, 50 : 50, 75 : 25, and 100 : 0), hydrated with sterile distilled water (1 : 3, w:v), and fermented for 72 h/37°C using Lactobacillus p...

Fermented milks play a major role in human diets worldwide. Lactic acid bacteria are the main microorganisms involved and often added as starters. However, use of autochthonous LAB strains for directed fermentations demands caution due to their potential to produce undesirable metabolites or harbor antibiotic resistance genes. The aim of this study...

RESUME Objectifs : L'objectif de cette étude est d'évaluer l'influence de quelques sources de contamination bactériologiques et d'identifier quelques entérobactéries présentes sur la laitue cultivée à Maroua. Méthodologie et résultats : Les échantillons d'eau, de sol et de laitue ont été prélevés sur 10 sites de production de la laitue dans la vill...

The present study had the objective to assess the ochratoxin A content of coffee through chromatographic analysis and design a method using PCR-DGGE to analyze at the same moment the totality of fungal flora present in the coffee samples in order to determine their geographic origin. 96 samples of coffee were collected from the west region (Bafouss...

The aim of this study was to determine the proximal composition and the fatty acid profile of fresh and smoked fillets of Pangasius hypophthalmus. After brining (4% / 270 min), the salted fillets of Pangasius hypophthalmus were pre-dried at 30°C for 30 min, then smoked at 30°C / 120 min; 50°C/ 240 min and at 80°C for 240 min. The proximal compositi...

The aimed of this study to evaluate the antifungal activity of essential oils of Ocimum basilicum L. on some fungi isolated from commercial rice sold in Cameroon. Fungal isolates were primarily identified based on morphological characteristics, while representative isolates were identified using PCR-based methods. Essential oils were extracted by h...

Aim: The aim of this study was to evaluate the sanitary quality of smoked fish by determining their mycotoxin content, and also the chemical composition, antifungal activity of Cymbopogon citratus and Ocimum basilicum essential oils from Cameroon against some mycotoxigenic fungal strains responsible of the smoked fish biodegradation. Place and Dura...

With a view to finding alternative compounds to the synthetic chemicals used in food preservation, a study was initiated to evaluate the antifungal activity of C. citratus essential oil on toxigenic molds isolated from Cameroonian rice. To this end, 12 rice samples of one kilogram each was taken in October 2019 in four cities in Cameroon: Tonga, Nd...

This study aims at bringing to light the preservative potential of essential oils of Thymus vulgaris, Cinnamomum zeylanicum and Mentha piperita in the stabilisation of lipids against oxidation. The essential oils were extracted by hydrodistillation and chemical composition were analysed by gas chromatography coupled with mass spectrometry. In vitro...

The aim of this study was to determine the chemical composition of the liquid smoke flavouring obtained from the pyrolysis of five tropical kinds of wood. Chemical characterization was performed by FT-IR and GC/MS on liquid smoke fractions soluble in dichloromethane. FT-IR spectroscopy revealed the presence of specific functional groups and chemica...

Raw cow's milk produced locally is a popular commodity but, its quality is a hindrance for the consumers. The objective of this study was firstly to identify the Staphylococcus present in raw cow's milk collected in Maroua (Cameroon) and, secondly to assess their level of resistance to antibiotics. Physico-chemical analyses based on the temperature...

Taking poorly stored antibiotics and not respecting their dosage and various contaminations can cause bacterial resistance. The aim of this study was to evaluate the digestive carriage of beta-lactamases with a extended spectrum in patients attending the Hospital of the Medico-social Center of the CNPS (Casse Nationale de Prevoyance Sociale) in Mar...

This study consists of analyzing the phytochemical composition, antioxidant activity and sensory properties of green tea infusions of two varieties of Ipomea batatas and two commercial green teas. Green tea was obtained after withering, fixing, rolling, and drying of I. batatas leaves. Phytochemical screening was performed using characteristic test...

Synopsis This book covers all areas of biotechnology, genetics and other related fields. The contributions by the authors include biosurfactants; antimicrobial activity; bio-preservation; shelf life; plasmid; algal plasmid; plasmid like DNA; fungal contamination; mycotoxins; Detarium microcarpum; Brachystegia eurycoma; Huanglongbing disease; Candid...

Bacteria generally ferment milk but, sometimes, yeasts are found in fermented milks. The presence of these yeasts in the microbial community of some fermented milks could be intentional or accidental. The Diversity of yeasts in the products was investigated using a molecular technique employing variable regions of 26S rDNA profiles generated by PCR-...

Combinations of equal volumes of essential oils of Ocimum gratissimum, Lippia rugosa and Xvlopia aethiopica were studied for their inhibiting activity on the mycelial growth of Aspergillus flavus by the determination of the minimum inhibiting concentrations (MIC), and on Sitophilus zeamais by the determination of the LV50 (volume that kills 50% of...

The aim of the present study was to investigate the essential oil of Cinnamomum zeylanicum from Cameroon for its chemical composition, antiradical and antifungal activities against some common fungi causing spoilage of stored food product. The essential oil, obtained by hydrodistillation of fresh leaves, was analysed by GC and GC/MS. The oil contai...

In the North of Cameroon, Boswellia dalzielii is a medicinal plant used in traditional medicine to treat infectious diseases (dysentery, typhoid, cholera and malaria). The aim of this study is to identify and characterized the bioactive compounds from the roots of this plant. The methanol crude extract from the root of Boswellia dalzielii Hutch was...

In order to contribute to the diversification of the range of probiotic products, limited until now to the dairy matrices, and to valorize the local raw materials of Cameroon, we set ourselves the objective of finding the conditions of fermentation of the fresh sap of Rafia farinifera by two probiotic ferments, namely, L. fermentum and B. bifidum....

This study aimed to analyze the phytochemical composition, antioxidant capacity, antimicrobial activity and the phenolic profile of Aframomum melegueta and Afrostyrax lepidophyllus methanolic extracts. The mean phenolic compound contents were 160.77 ± 6.74 and 46.63 ± 0.93mg GAE/g extract, while that of the tannins was 136.40 ± 0.94 and 8.72 ± 1...

Onion powder is a worldwide food ingredient with great commercial value due to its culinary and medicinal properties in “Nutraceuticals”, which has not been investigated. This study aimed at evaluating the effect of drying treatment on the nutritional, functional and sensory properties of onion powder from three varieties (Violet of Galmi, Goudami...

Microbiological and physico-chemical quality of fermented milks consummed in Maroua (Cameroun) ABSTRACT The non respect of hygiene regulation during the production of fermented milks could sometimes lead to a contamination of products by potentially pathogenic microorganisms. It is from this perspective that a study was realized with the aim of as...

Methanol extracts of five spices from Cameroon were analyzed for their phytochemical composition and antiox- idant activities. The Xylopia aethiopica extract with the highest total phenolics and flavonoids contents showed a strongest antioxidant activity compared to tert-butylhydroquinone (TBHQ) using both the 1,1-diphenyl-2- picrylhydrazyl (DPPH)...

Background: In Cameroon, most peoples use traditional medicine treating infectious diseases. To verify the scientific bases of these locally used medicinal plants, an ethnobotanical survey was carried out in some villages of Vina and Mayo-Louti Divisions. Materials and Methods: Interviews were conducted through structured questionnaires among 31 tr...

Dans l'optique de contribuer à la diversification de la gamme des produits probiotiques limités jusqu ici aux matrices laitières et de valoriser les matières premières locales camerounaises, nous nous sommes fixés pour objectif de trouver les conditions optimales de fermentation de la sève fraiche de Rafia farinifera par deux ferments probiotiques...

Sciences Alimentaires et Nutrition, BP 454, Ngaoundéré, Cameroun 4 CIRAD (Centre de coopération internationale en recherche agronomique pour le développement), Département des Performances des systèmes de production et de transformation tropicaux. UMR 95 QUALISUD. Maison de la Technologie TA B-95/16, Résumé Cette étude a été réalisée dans le but d'...

The purpose of this study was to assess the nutritional properties of Bambara milk and yoghurt. The milk was produced by aqueous extraction of flour obtained by dehulling the seeds followed by parboiling. It was then fermented using a mix culture of L. Bulgaricus, S. thermophilus and L. plantarum, followed by the evaluation of the protein biologica...

. The present study aimed to evaluate the proximate composition and functional properties of Cucurbita maxima, Cucumeropsis mannii and Lagenaria sciceraria defatted seed flours. The dehulled seeds were dried at 45 °C for 24 h, defatted using hexane and crushed to 500 µm to produce the defatted flour. Results revealed that they contain around 70% of...

The aim of this work was to evaluate the chemical composition, antiradical activity and antifungal activity of Xylopia aethiopica essential oil from Cameroon against five mycotoxigenic fungal strains responsible for the biodegradation of foodstuffs. The Clevenger apparatus was used for the extraction of essential oil from dried fruits, which was su...

The aim of this research was to study the parameters influencing moisture, salt and total phenol content of hot smoked Nile Perch and to compare the influence of processing conditions on its proximate composition and sensory quality. The effect on moisture, salt and total phenol content was investigated using fractional factorial design with seven...

This study aimed to evaluate the proximate composition, physical characteristics and water absorption kinetics of five cucurbitaceae seeds species (Cucumeropsis mannii, Cucumis sativus, Cucurbita maxima, Cucurbita moschata and Lagenaria siceraria) consumed in Cameroon. The results revealed that the proximate composition of seeds changed significant...

To fight against thirst, peoples in sahelian regions have many choices: they can drink water or kinds of beverages like "kounou", lemon juice, tamarind juice baobab juice, tea or "folere" juice. These beverages have been prepared with wells water, drilling water or with the water of the national network distribution. The aim of this study was to ev...

Nnam gon, a cake made by steam cooking a mixture of Cucurbitaceae seeds paste and others ingredients specially spices, is a highly prized dish in Central African region. A preliminary investigation conducted as part of this study highlighted that formulations used in the processing of Nnam gon vary according to the spices used. This study was carri...