Tatiana Troshina

Life-line Clinic Moscow · embryology

Objective To evaluate the influence of aspiration volume and final sperm position relative to the needle on fertilization and embryo development competence in ICSI. Methods A retrospective study analyzed 385 ICSI procedure videos, measuring distances between the needle bevel base and the sperm at oolemma breakage and needle withdrawal moments. Out...

Study question Is there any difference in PGT-A results between good quality blastocysts depending on whether they reach this stage on the fifth or sixth day? Summary answer In patients over 36 years old, there is a significantly higher rate of aneuploidy in embryos that reach the blastocyst stage on Day 6. What is known already The level of chro...

Several important morphogenetic processes belong to the category of collective cell movements (CCM), by which we mean coordinated rearrangements of many neighboring cells. The causes of the dynamic order established during CCM are still unclear. We performed statistical studies of rates and angular orientations of cell rearrangements in two kinds o...

Although the folding of epithelial layers is one of the most common morphogenetic events, the underlying mechanisms of this process are still poorly understood. We aimed to determine whether an artificial bending of an embryonic cell sheet, which normally remains flat, is reinforced and stabilized by intrinsic cell transformations. We observed both...

Computer analysis of artificially deformed (stretched or compressed) double explants (sandwiches) of the blastocoel roof (BRs) and suprablastoporal region (SBRs) of African clawed frog Xenopus laevis early gastrula has been performed using frames of time-lapse microfilming. During the first 14 min after cutting off, the velocities and displacement...

The mutual arrangement of neural and mesodermal rudiments in artificially bent double explants of Xenopus laevis suprablastoporal areas was compared with that of intact explants. While some of the bent explants straightened or became spherical, most retained and actively reinforced the imposed curvature, creating folds on their concave sides and ex...

Sandwich explants of the suprablastoporal area of Xenopus early-mid gastrula and same stages of entire embryos were stretched with two needles perpendicular to the direction of natural elongation of the axial rudiments. The changes in the embryonic shape and histological structure were monitored as well as the arrangement of descendants of one of d...

Studies of mechanical stresses and mechanical feedback at the cell level are reviewed. It is shown that cells and embryonic tissues respond to external mechanical stresses and can generate such stresses themselves. Regular feedback loops between external (passive) and internal (active) mechanical stresses have been established. They are essential f...

This study explored the integral intensity, slopes, and dynamics of the spectral gels, dried collagen samples, and collagen fibrils extirpated from rat tails after UV emitted from dry collagen samples are more exponential, whereas water-