Tatiana Gordeeva

Tatiana Gordeeva
State Research Institute of Genetics and Selection of Industrial Microorganisms | Genetika · Department of Genetics

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32
Publications
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139
Citations

Publications

Publications (32)
Article
The phyCg gene encoding a new phytase from C. gillenii was optimized, synthesized, cloned, and expressed in Pichia pastoris. Analysis of the amino acid sequence of the enzyme showed that it belongs to the histidine acid phosphatase family. The amino acid sequence of the PhyCg phytase has the highest homology (73.49%) with a phytase sequence from Ci...
Article
The isolation, heterologous gene expression, and characteristics of a new β-glucanase from Bacillus pumilus is described. The bgl1 gene from the Bacillus pumilus Bg57 VKPM В-13195 strain, which consists of 729 nucleotides, encodes a secreted endo-β-l,3(4)-D-glucanase (EC 3.2.1.6) containing 214 amino acids and 28 residues of the putative signal pep...
Article
The heterologous expression, isolation, and characterization of a novel xylanase from Paenibacillus brasilensis are described. The xyl1 gene from the Paenibacillus brasilensis strain X1 VKPM B-13092, which consists of 639 nucleotides, encodes a secreted endo-1,4-β-xylanase (EC 3.2.1.8) containing 184 amino acids and 28 residues of the putative sign...
Article
Endo-1,4-β-xylanases from Bacillus pumilus VKPM В-7975, Paenibacillus polymyxa VKPM В-3015, Thermomyces lanuginosus VKPM F-224, and Schizophyllum commune VKPM F-433, which are members of a family of 11 glycohydrolases, have been obtained in the expression system of Pichia pastoris. Technologically valuable characteristics of the recombinant hydrola...
Article
Phytases are widely used to improve the properties of mixed fodder. Due to its high specific activity, PhyА-Cf phytase from Citrobacter freundii is of special interest for agrobiotechnology. In order to decrease the thermal sensitivity of the enzyme during fodder granulation at 60–80°C, the thermal stability of phytase was enhanced by site-directed...
Article
In¹ order to increase the expression level of gene for recombinant phytase in Pichia pastoris, a synthetic sequence of the phyOp-mod gene, which encodes phytase from Obesumbacterium proteus, has been designed and optimized by its codon composition. The GC content was adjusted to 48.33%, and AT-rich segments were eliminated. The main characteristics...
Article
The expression of four genes of bacterial endo-β-l,3(4)-D-glucanases from B. pumilus Bg57 (VKPM В-13195), Paenibacillus polymyxa Bg23 (VKPM В-4056), Bacillus subtilis Bg11 (VKPM B-13178), and Bacillus amyloliquefaciens Bg76 (VKPM B-13184), which are members of the family of GH16 glycohydrolases, in the expression system of Pichia pastoris are compa...
Article
The heterologous expression and characteristics of a new xylanase from Pyromyces finnis have been described. The endo-l,4-β-xylanase XylP (EC 3.2.1.8) consists of 223 amino acids and 19 residues of a putative signal peptide in the N-terminal region. The amino acid sequence of the mature protein has the greatest homology with the sequence of the nat...
Article
The isolation, heterologous expression and characterization of a new thermostable β-glucanase from Paenibacillus jamilae is described. The bgl26 gene from the P. jamilae Bg1 VKPM B-13093 strain consisting of 714 nucleotides encodes endo-1,3-1,4-β-glucanase (EC 3.2.1.73) containing 213 amino acids and 24 residues of the putative signal peptide in N-...
Article
The co-expression of genes encoding β-glucanases from Paenibacillus jamilae and Rhizomucor miehei in the Komagataella kurtzmanii methylotrophic yeast system has been carried out. The recombinant strains that simultaneously produced both enzymes with a total activity of 1132 and 1464 units for 96 h of cultivation in flasks were obtained. The combina...
Article
The genes for bacterial phytases from Citrobacter freundii and Yersinia intermedia were expressed for the first time in a thermotolarant yeast Ogataea polymorpha. A comparative analysis of the properties of recombinant phytases produced by Ogataea polymorpha and Pichia pastoris yeasts was carried out. It was shown that the stability, pH and tempera...
Article
A Pichia pastoris yeast strain producing endo-l,4-β-xylanase from Paenibacillus brasilensis with an activity of 54,400 U/mL after 140 h of fermentation in a laboratory fermenter has been obtained. A number of approaches were used to increase the level of the xylanase production in this strain: optimization of the target gene codon composition, mult...
Article
A method is proposed for the rapid identification of the taxa of five industrially valuable yeast groups: Pichia pastoris, Yarrowia lipolytica, Schizosaccharomyces pombe, Saccharomyces cerevisiae, and Kluyveromyces lactis. The specific primers selected based on nucleotide sequences of genes encoding rRNA were used in PCR. The method was effective i...
Article
A stepwise screening of bacterial xylanase producers isolated from forest soil in Moscow oblast has been performed, and the taxa of the microorganisms have been identified based on an analysis of the 16S RNA gene sequences. An approach to the selection of the most active producers is suggested, and this selection has been carried out, resulting in...
Article
Comparative analysis of the efficiency of the expression of the Escherichia coli, Obessumbacterium proteus, and Citrobacter freundii phytase genes in Pichia pastoris methylotrophic yeast has been carried out. A simple model with a genetic construct based on the pPICά vector and the plate test with a modified diagnostic medium containing methanol (i...
Article
The expression of the genes for endo-1,4-β-xylanase from Bacillus pumilus VKPM â-7975, Paenibacillus polymyxa VKPM â-3015, Thermomyces lanuginosus VKPM F-224, and Schizophyllum commune VKPM F-433, members of the family of 11 glycohydrolases, in the expression system of Pichia pastoris has been assessed. Such technologically valuable characteristics...
Article
In order to increase the level of expression of recombinant phytases in the Pichia pastoris yeast, a synthetic sequence of the phyOp-mod gene that encodes phytase from Obesumbacterium proteus has been developed and optimized by codon composition. The GC content was adjusted to 48.33, and the AT-rich segments were eliminated. The main characteristic...
Article
The isolation, heterological gene expression and characteristics of a new β-glucanase from Bacillus pumilus has been described for the first time. A gene bgl1 from the Bacillus pumilus Bg57 VKPM В-13195 consisting of 729 nucleotides encodes a secreted endo- β-l,3(4)-d-glucanase ( EC 3.2.1.6) that contains 214 amino acids and 28 residues of the puta...
Article
В экспрессионной системе Pichia pastoris проведено сравнение четырех бактериальных эндо-β- l,3(4)-D-глюканаз из Bacillus pumilus Bg57 ВКПМ В-13195, Paenibacillus polymyxa Bg23 ВКПМ В-4056, Bacillus subtilis Bg11 ВКПМ B-13178 и B. amyloliquefaciens Bg76 ВКПМ B-13184, относящихся к семейству GH16 гликозилгидролаз. Были исследованы такие технологическ...
Article
Описана изоляция, гетерологическая экспрессия и характеристика новой ксиланазы из Paenibacillus brasilensis. Ген xyl1 из штамма P. brasilensis X1 ВКПМ В-13092, состоящий из 639 нуклеотидов, кодирует эндо-1,4-β-ксиланазу (EC 3.2.1.8) из 184 аминокислот и 28 остатков предполагаемого сигнального пептида в N-концевой области. Нуклеотидная последователь...
Article
Фитазы широко используются для улучшения питательных свойств комбикормов. Благодаря высокой удельной активности фитаза PhyА-Cf из Citrobacter freundii представляет особый интерес для агробиотехнологии. Для снижения инактивации фермента во время гранулирования корма при 60-80 °С была увеличена термостабильность фитазы PhyА-Cf методом сайт-направленн...
Article
A stepwise screening of bacterial producers of xylanases isolated from the forest soil in Moscow oblast, and also the taxonomic identification of the microorganisms based on the analysis of the 16S RNA gene sequences have been performed. An approach to the selection of the most active producers was suggested, and this selection was carried out resu...
Article
A method for a rapid taxonomy identification of five industrially valuable yeast groups, Pichia pastoris, Yarrowia lipolytica, Schizosaccharomyces pombe, Saccharomyces cerevisiae and Kluyveromyces lactis, has been suggested. The specific primers that were selected on the basis of nucleotide sequences of genes encoding rRNA were used in PCR. The met...
Article
A comparative analysis of the efficiency of the Escherichia coli, Obessumbacterium proteus and Citrobacter freundii phytase gene expression in a Pichia pastoris methylotrophic yeast has been carried out. The level of the expression was assessed using a suggested for the first time simple model using a genetic construct based on the pPICά vector and...
Article
The expression of L-lactate dehydrogenase genes ldh1 (Bos taurus), ldhA (Homo sapiens), ldhA (Rhizopus oryzae), ldh1 (Lactobacillus plantarum), and ldh1 (Lactobacillus pentosus) in the cells of yeast Schizosaccharomyces pombe VKPM U-3106 has been investigated. The catalytic characteristics of the enzymes encoded by these genes have been compared, a...
Article
Full-text available
An efficient and inexpensive spectrophotometric method has been developed for the determination of lactic acid in the individual state and in food and biological and cultural liquids. The method is based on the spectrophotometric determination of the colored product of the reaction of lactate ions with iron(III) chloride at 390 nm. The optimum cond...

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