Tatiana El-BachaFederal University of Rio de Janeiro | UFRJ · Institute of Nutrition
Tatiana El-Bacha
DSc
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45
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Introduction
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Publications
Publications (45)
Gestational Diabetes Mellitus (GDM) is the most common medical complication of pregnancy, and a severe threat to pregnant people and offspring health. The molecular origins of GDM, and in particular the placental responses, are not fully known. The present study aimed to perform a comprehensive characterization of the lipid species in placentas fro...
We investigated whether gestational diabetes mellitus (GDM) associated with maternal obesity modifies the placental profile of F4-Neuroprostanes and F2-Isoprostanes, metabolites of non-enzymatic oxidation of docosahexaenoic acid (DHA) and arachidonic acid (AA), respectively. Twenty-five placental samples were divided into lean (n=11), obesity (n=7)...
The rise in prevalence of obesity in women of reproductive age in developed and developing countries might propagate intergenerational cycles of detrimental effects on metabolic health. Placental lipid metabolism is disrupted by maternal obesity, which possibly affects the life-long health of the offspring. Here, we investigated placental lipid met...
The rise in prevalence of obesity in women of reproductive age in both developed and developing countries might propagate intergenerational cycles of detrimental effects on metabolic health, contributing to substantial economic burden on society. Placental lipid metabolism might be disrupted by maternal obesity, which possibly affects the life-long...
Structured lipids (SL) represent a new generation of lipids, considered bioactive compounds. Medium-chain, oleic (18:1n-9), and medium-chain fatty acid (MCFA) structured lipids (MOM-SL) were produced by acidolysis reaction in solvent-free medium with capric (10:0) and lauric (12:0) free fatty acids (FFAs) and triolein or olive oil, using Yarrowia l...
Objectives
To investigate the effects of commercial avocado powder (Hass variety) during pre-conception and gestation on metabolic and reproductive aspects of C57BL/6J mice dams.
Methods
Female C57BL/6J mice were kept on either a control (AIN 93; CON) or avocado (dextrose and sucrose replacement with 25% commercial avocado power; AVO) isoenergetic...
Our aims were to investigate vitamin A and E status during lactation and the determinants of breast milk content for the appropriate nutrition of the infant in a study with nursing Brazilian women. We hypothesized that both inadequate intake and the lipoprotein distribution of vitamin A and E during lactation could have an impact on their breast mi...
Cancer cells demand high ATP provisions to support proliferation, and targeting of energy metabolism is a good strategy to increase their sensitivity to treatments. In Brazil, wine manufacture is expanding, increasing the amount of pomace that is produced. We determined the phenolic composition and antioxidant properties of a dark skin Grape Pomace...
We combined ¹H NMR metabolomics with functional and molecular biochemical assays to describe the metabolic changes elicited by vitamin D in HEK293T, an embryonic proliferative cell line adapted to high-glucose concentrations. Activation of the polyol pathway, was the most important consequence of cell exposure to high glucose concentration, resembl...
Glutamine is a conditionally essential amino acid that is an important metabolic resource for proliferating tissues by acting as a proteinogenic amino acid, a nitrogen donor for biosynthetic reactions and as a substrate for the citric acid, or tricarboxylic acid (TCA), cycle. The Human Immunodeficiency Virus type 1 (HIV-1) productively infects acti...
The quality of dietary lipids in the maternal diet can programme the offspring to diseases in later life. We investigated whether the maternal intake of palm oil or interesterified fat, substitutes for trans -unsaturated fatty acids (FA), induces metabolic changes in the adult offspring. During pregnancy and lactation, C57BL/6 female mice received...
Dietary patterns (DPs) have been described as an important factor that may influence polyunsaturated fatty acid (PUFA) concentrations and body mass index (BMI) during pregnancy. We aim to evaluate the association between pre-pregnancy DPs and serum PUFA percentages throughout pregnancy considering early pregnancy BMI as a possible effect modifier....
Aims/hypothesis:
The aim of this work was to determine whether placental endoplasmic reticulum (ER) stress may contribute to the pathophysiology of gestational diabetes mellitus (GDM) and to test the efficacy of chemical chaperones and antioxidant vitamins in ameliorating that stress in a trophoblast-like cell line in vitro.
Methods:
Placental s...
Importance:
Dengue virus infection, due to its global burden, is the most important mosquito-born viral disease. There is no specific treatment for dengue disease and early detection lowers fatality rates to below 1%. In this study we observed the effects of dengue virus infection on the profile of small molecules in the blood of patients with mil...
Phospholipids are ubiquitous molecules that participate in innumerous cellular events. The biochemical and physiological properties of phospholipids are largely related to their amphipathic feature. Mammalian cell membranes contain more than 1000 different phospholipids. Phospholipids exert structural functions in cellular membranes, which vary in...
Infectious diseases such as those caused by virus, account for a vast proportion of deaths worldwide. Re-emerging aspects of host-virus interactions in recent literature include the vital role played by host metabolism on viral replication and the pro-active participation of mitochondria in this process. Different viruses use distinctive strategies...
The metabolic resources crucial for viral replication are provided by the host. Details of the mechanisms by which viruses interact with host metabolism, altering and recruiting high free-energy molecules for their own replication, remain unknown. Sindbis virus, the prototype of and most widespread alphavirus, causes outbreaks of arthritis in human...
Sodium butyrate and trichostatin A induce time and dose-dependent inhibition of growth in H460 cells. Cells were grown in the absence or presence of various concentrations of NaB (1, 3 and 10 mM; A–C) or TSA (0.02, 0.2 and 1 µM; D–F) for 24 or 48 h. At indicated times, cell number and viability were analyzed by trypan blue exclusion (A and D) and M...
HK I is bound to mitochondria in H460 cells. Control (A and C) or NaB treated (B and D) H460 cells were labeled with antibodies against mitofusin I (A–B) or mitofusin II (C–D) (red), Hexokinase I (green). Merge HK + Mfn (yellow) and DAPI (Blue). Representative images taken with an inverted fluorescence microscope Zeiss AxioObserver Z1 are shown. Ba...
List of primer sequences used for quantitative Real Time PCR.
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ATP Content.
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Immunocytochemistry.
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Sodium butyrate induces F-actin rearrangements in H460 cells. H460 cells were gown in the absence (A) or presence (B) of 10 mM NaB. Cells were labeled with rhodamine-conjugated phaloidin (red) and DAPI (blue), to allow visualization of actin citoeskeleton and nuclei, respectively. Representative immunofluorescence images obtained using a Zeiss Axio...
Sodium butyrate and trichostatin A induces cell cycle arrest and inhibits cellular DNA synthesis. H460 cells were treated or not with 3 or 10 mM NaB (A) and 0.2 µM TSA (B) for 24 h. Posteriorly cells were harvested, permeabilized, incubated with propidium iodide and had its DNA content analyzed by flow cytometry using FACScan. Values represent mean...
Trichostatin A reduces lactate production in H460 cells. After 24 h of treatment with 0.2 µM TSA, H460 cells were incubated with glucose-supplemented medium. Aliquots of supernatants were collected every 10 min. and incubated in hidrazine buffer pH 9.2, with an excess of NAD+ and lactate dehydrogenase (LDH) for measurement of lactate release. (A) K...
High-resolution respirometry shows an increase in oxidative metabolism subsequent to treatment with trichostatin A. (A) Representative record of oxygen concentration and flow of intact H460 cells treated or not with 0.2 µM TSA for 24 h. During the assay, cells were maintained in RPMI medium with glucose and without FBS. Black dashed line represents...
Effect of sodium butyrate on ATP content in H460 cells. ATP content was assayed using an enzymatic method with hexokinase and glucose-6-phosphate dehydrogenase [40]. Values represent mean ± SEM; N = 3.
(DOC)
Background:
Tumor cells are characterized by accelerated growth usually accompanied by up-regulated pathways that ultimately increase the rate of ATP production. These cells can suffer metabolic reprogramming, resulting in distinct bioenergetic phenotypes, generally enhancing glycolysis channeled to lactate production. In the present work we showe...
Recent results obtained from research on the intermediary metabolism of tumor cells have uncovered the biochemical reprogramming that takes place upon malignant transformation. Many features have been highlighted that are currently being exploited for specific chemotherapy. Many more will become available shortly as a consequence of the recognition...
This work describes the use of a virtual learning environment (VLE) applied to the biochemistry class for undergraduate, first-year medical students at the Federal University of Rio de Janeiro. The course focused on the integration of energy metabolism, exploring metabolic adaptations in different physiological or pathological states such as starva...
Dengue virus (DENV) infection is considered the major human arbovirosis, which affectsmillions of people in tropical urban centers leading to thousands of deaths annually. Theclinical presentations of DENV infection range from asymptomatic, or a mild self-limitedillness, dengue fever (DF), to severe and potentially life-threatening diseases, dengue...
Liver damage occurs during Dengue Virus infection and constitutes a characteristic of severe forms of the disease. The present study was focused on the modulation of gene expression in a human hepatic cell lineage, HepG2, in response to Dengue Virus infection.
The global gene expression changes in HepG2 cells after 6, 24 and 48h of infection with D...
As Information and Communication Technology (ICT) becomes available in educational contexts, it is important that educators experiment different ways to deal with ICT tools in the teaching -learning process at the University basic sciences level. The challenge is to integrate ICT throughout the learning subjects in order to improve the quality of t...
3-BrPA (3-bromopyruvate) is an alkylating agent with anti-tumoral activity on hepatocellular carcinoma. This compound inhibits cellular ATP production owing to its action on glycolysis and oxidative phosphorylation; however, the specific metabolic steps and mechanisms of 3-BrPA action in human hepatocellular carcinomas, particularly its effects on...
Dengue virus infection affects millions of people all over the world. Although the clinical manifestations of dengue virus-induced diseases are known, the physiopathological mechanisms involved in deteriorating cellular function are not yet understood. In this study we evaluated for the first time the associations between dengue virus-induced cell...
Vaccine discovery stands out as one of the public health interventions that has achieved the greatest impact in world's health. Vaccination is the most effective means of disease prevention, especially for viral infections. Starting with the use of smallpox vaccine by Jenner in the late 1700s, the technology for vaccine development has seen numerou...
Although it is well established that cellular transformation with tumor virus leads to changes on glucose metabolism, the effects of cell infection by non-transforming virus are far to be completely elucidated. In this study, we report the first evidence that cultured Vero cells infected with the alphavirus Mayaro show several alterations on glucos...
Neoplastic cells generally present profound changes in glucose metabolism. The mechanisms underlying such process are numerous and all may involve altered cellular hormonal responses. Here we report the first evidence that cellular location of phosphofructokinase activity in human breast cancer tissues is different from the one observed in control...
Phosphofructokinase-1 plays a key role in the regulation of carbohydrate metabolism. Its activity can be used as an indicator of the glycolytic flux in a tissue sample. The method most commonly employed to determine phosphofructokinase-1 activity is based on oxidation of NADH by the use of aldolase, triosephosphate isomerase, and alpha-glycerophosp...
Projects
Projects (2)
The goal of our laboratory is to provide a better understanding of the mechanisms by which cells respond and adapt to different environmental stresses. We are particularly interested on how mitochondria participate in these stress signaling responses, what are the structural and functional consequences to this organelle, as well as the implications to cell physiology and survival.
Influence of TXNIP over-expression on metabolism by NMR metabolomics
