Tatas Brotosudarmo

Tatas Brotosudarmo
Universitas Ciputra Surabaya · Department of Food Technology

PhD

About

152
Publications
56,404
Reads
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994
Citations
Introduction
I am interested in plants and microbial pigments, which are related to their structures, functions, and regulations. I use various methods, especially mass spectrometry, and fluorescence spectroscopy as tools for observation. Note: For those of you who want to get our published journals, please click the DOI link. Later you will be given access to the provider directly. Sorry I will not upload the pdf file here because it violates copyright.
Additional affiliations
February 2022 - present
Universitas Ciputra Surabaya
Position
  • Professor (Associate)
April 2011 - January 2022
Ma Chung University
Position
  • Principal Investigator
April 2011 - December 2022
Ma Chung University
Position
  • Professor (Associate)
Education
February 2007 - February 2010
University of Glasgow
Field of study
  • Biochemistry
October 2003 - November 2006
Ludwig-Maximilians-University of Munich
Field of study
  • Physical Chemistry

Publications

Publications (152)
Article
Fucoxanthin, an algal carotenoid with bioactivity that benefits human health, is gaining popularity as a functional food. Therefore, in this study, the effect of drying methods and conditions on the production of fucoxanthin isomers in three closely related Sargassum species with slightly different morphological lamina were investigated. The recove...
Conference Paper
The dregs of green teas have recently been investigated as a potential source of pigments which exhibits antibacterial effects via the photodynamic mechanism. The dregs contain chlorophyll derivatives and trace carotenoids, which can absorb visible lights and produce cytotoxic radicals. The present research was aimed to perform: (i) the photostabil...
Article
The siphonaxanthin-siphonein-Chl-a/b-protein (SCP) complex from the siphonous green alga Codium fragile is the major light-harvesting complex (LHC) of these alga and is highly homologous to that of green plants (trimeric pigment-protein complex, LHCII). Interestingly, we find remarkable differences in the spectral response from individual SCP compl...
Article
Full-text available
The siphonaxanthin-siphonein-Chl-a/b-protein (SCP) is the light-harvesting complex of the marine alga Codium fragile. Its structure resembles that of the major light-harvesting complexes of higher plants, LHC II, yet it features a reversed Chl a:Chl b ratio and it accommodates other variants of carotenoids. We have recorded the fluorescence emissio...
Article
The headspace solid phase microextraction (HS-SPME) technique has been recognized as a reliable technique for characterizing the aroma profile of Arabica coffee beans. The amount and content of the detected volatile compounds depend on the volatile analyte extraction process with HS-SPME, namely the adsorption and desorption processes. However, the...
Article
Simultaneous purification of fucoxanthin isomers from brown seaweeds by two steps of open-column chromatography (OCC) and reversed-phase (RP)-high-performance liquid chromatography (HPLC) is described. Analysis and identification of fucoxanthin isomers were performed by chromatographic and spectrophotometric properties such as retention time (tR),...
Article
Full-text available
Cincalok is a typical food from West Kalimantan made from fermented rebon shrimp containing astaxanthin, the most potent antioxidant in nature. This study investigated an efficient method for extracting astaxanthin from the cincalok using vegetable oils as solvents. Olive, sesame, grape seeds, coconut, and virgin coconut oil were used as alternativ...
Article
Full-text available
Currently, there are only six species in the genus Seonamhaeicola, i.e., Seonamhaeicola aphaedonensis, S. algicola, S. marinus, S. acroporae, S. maritimus, and S. sediminis. These bacteria have typical yellow or orange color. Among the identified strains, only S. marinus that had been reported to have a yellow polyene flexirubin pigment. However, t...
Article
Full-text available
Marine microbes offer a significant source for biodiscovery due to their rich biodiversity and genetic capacity. Particularly, microbial pigments of marine origin are getting more attention in current research due to their widely perceived application as natural food colorants, antioxidant, antimicrobial, and many more. In the past five years, our...
Article
Full-text available
Rapid assessment of plant photosynthetic pigments content is an essential issue in precise management farming. Such an assessment can represent the status of plants in their stages of growth. We have developed a new 2 Dimensional-Convolutional Neural Network (2D-CNN) architecture, the P3MNet. This architecture simultaneously predicts the content of...
Article
Full-text available
The production of tea dregs is continually increasing along with the growth of people's interest in ready-to-drink beverages. However, the recent development of research on the use of tea dregs is still very limited. The present study was aimed to identify the remnant photosynthetic pigments in tea dregs, determine their composition, and evaluate t...
Article
Full-text available
As vitamin A deficiency (VAD) remains a health problem in Indonesia, fulfilling the requirement of vitamin A from the daily diet is of importance, especially for children. Consuming food sources rich in provitamin A carotenoids, such as bananas and yellow-fleshed sweet potatoes, is one alternative to reduce the number of VADs. Raw materials have be...
Article
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Chlorophyll and its derivatives are potential natural sensitizers frequently applied in antimicrobial photodynamic therapy. Chlorophyll derivatives are formed naturally during tea processing, but they do not contribute to the color of tea infusions and thus are presumably left in the tea dregs. The present study aimed to investigate (i) the chlorop...
Article
Full-text available
Microbial pigments of marine origin are gaining increasing attention in current research due to their widely perceived applications as natural food colorants, antioxidants, antimicrobial compounds, anticancer agents, and immune stimulators. This review is a short acknowledgement of the significant progress achieved over the past five years of studi...
Article
β-carotene is an important nutritious content in banana. However, its lifetime depends on the enzymes controlling its conversion into strigolactone. To understand the involved enzymes’ activity, which are β-carotene isomerase (D27), carotenoid cleavage dioxygenase 7 (CCD7), and CCD8, would be the key to manipulate the rate of β-carotene degradation...
Article
Full-text available
Banana, as the world’s most consumed fruit, is a good source of carbohydrate, potassium, fiber, and other essential nutrients such as pro-vitamin A carotenoids. The carotenoid content and composition in bananas commonly sold in fruit shops in Malang have become interesting studies. Five types of banana, i.e. Berlin, Mas, Cavendish, Candi and Raja w...
Article
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Urban green open space is areas in a city or town filled with vegetation to support socio-ecological functions. These areas have increasingly threatened as a result of being converted to urban infrastructures. As an essential feature of city infrastructure, urban green space should be monitored according to the spatial plan of the city area. Howeve...
Article
The composition of carotenoid pigments from the fruit of the Pandanus plant, Pandanus conoideus Lam. monocot or locally named as red fruit, indigenous to the Papua Islands, was investigated. By chromatographic and spectrometric analyses using RP-HPLC with C18 and C30 columns and gradient elution, NMR, MS/MS and FT-IR, eight κ-end group carotenoids,...
Article
Full-text available
Pigments from marine bacteria have attracted the attention for scientists because of their extensive applications and currently exploration of new pigment sources from marine bacteria is still ongoing. Recently, we have successfully isolated six new yellow-pigmented marine bacteria, strain PS2, PM2, SB11, SB13, SB21, and SB23, isolated from seawate...
Article
Full-text available
The assessment of the photosynthetic pigment contents in plants is a common procedure in agricultural studies and can describe plant conditions, such as their nutritional status, response to environmental changes, senescence, disease status and so forth. In this report, we show how the photosynthetic pigment contents in plant leaves can be predicte...
Article
Lutein is a type of xanthophyll that accumulated predominantly in eye macula, and is distributed in several parts of the human body, such as skin and brain. Lutein accumulation in the human body is associated with the health benefits of lutein, including being an antioxidant and protecting, the eyes and skin from exposure to blue light, and playing...
Article
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The emerging pandemic of Corona Virus Disease (COVID-19) has attracted special concerns regarding the case of very contagious viral infection. Besides the profound waiting toward vaccine development, public awareness was also addressed into the effort of increasing humans’ body immunity. Nowadays, the people are encouraged to consume adequate amoun...
Article
Full-text available
Reactive oxygen species (ROS) are continuously generated as a by-product of normal aerobic metabolism. Elevated ROS formation leads to potential damage of biological structures and is implicated in various diseases. Astaxanthin, a xanthophyll carotenoid, is a secondary metabolite responsible for the red-orange color of a number of marine animals an...
Article
Full-text available
Banana cultivars that are rich in provitamin A carotenoids and other nutrients may offer a potential food source to help alleviate vitamin A deficiencies, particularly in developing countries. The local plantain type banana, Agung Semeru (Musa paradisiaca L.), was investigated, in order to analyse the changes in the compositions of the provitamin A...
Article
Full-text available
Palm oil is known as one of the richest sources of carotenes. Carotenes concentrate could be prepared from crude palm oil and possessing potency to be used as nutraceutical materials. Due to the susceptibility of carotenes against heat, the encapsulation procedure might be applied to provide protections. The present study was aimed to learn the inf...
Article
Purification procedures to obtain light-harvesting complexes were performed on two photosynthetic bacteria, i.e. Rhodospseudomonas (Rps.) palustris and Rhodobacter (Rb.) sphaeroides. In this study, purification was initially carried out using series of centrifugation towards the bacteria cell to acquire chromatophore of the bacteria. Application of...
Article
Full-text available
Red pigmented marine bacteria, Pseudoalteromonas rubra strains PS1 and SB14, were isolated from two sampling locations in different ecosystems on Alor Island, Indonesia, and cultured in the laboratory. We analyzed the 16S rRNA gene sequences and examined the pigment composition and found that both strains produced cycloprodigiosin (3), prodigiosin...
Article
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The need for the rapid assessment of the photosynthetic pigment contents in plants has encouraged the development of studies to produce nondestructive quantification methods. This need is driven by the fact that data on the photosynthetic pigment contents can provide a variety of important information that is related to plant conditions. Using deep...
Article
Full-text available
Non-destructive measurement of plant chlorophyll concentration using the normalized difference vegetation index (NDVI) has long been standard practice to determine the plant health status. This is, because the NDVI value is correlated with the chlorophyll concentration which in turn is highly correlated with other vital plant parameters such as nit...
Article
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Current efforts to explore the potency of marine resources have been made to promote economic development in Indonesia, especially in the industrial sector. Marine cyanobacteria such as Arthospira sp. and Scytonema sp. are two potential candidates of the blue-green algae that can be used in the application for food and health industries. This study...
Article
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Suji (Pleomele angustifolia) is one kind of Indonesian typical plants which can be used as natural green food coloring agent. The susceptibility of natural pigment to external environment forces the protection in order to prolong its shelf life. Encapsulation has been known in the art of food preparation to provide protection for several ingredient...
Article
Full-text available
Excessive fat consumption can cause obesity, which is currently one of the global problems, because it can trigger various diseases such as coronary heart disease, diabetes, and so on. Absorption of fat into the body requires pancreatic lipase enzymes, which can be reduced by lipase inhibitors such as orlistat drugs. Previous research has shown tha...
Article
Full-text available
Erythrobacter flavus strain KJ5 (formerly called Erythrobacter sp. strain KJ5) is a yellowish marine bacterium that was isolated from a hard coral Acropora nasuta in the Karimunjawa Islands, Indonesia. The complete genome sequence of the bacterium has been reported recently. In this study, we examined the carotenoid composition of this bacterium us...
Article
Full-text available
Abstract. The objectives of this study were to isolate and analyze β-cryptoxanthin from yellow pigmented marine bacterium Erythrobacter sp. KJ5. The pigments from Erythrobacter sp. KJ5 were extracted from cells with methanol:acetone (7:3, v/v). β-cryptoxanthin standard was isolated from citrus fruit peel using 100% acetone and purified by high-perf...
Article
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AbstrakKelapa sawit (Elaeis guineensis Jacq.) merupakan tanaman yang mengandung karotenoid tinggi pada mesokarp yang merupakan bagian dari buah. Cahaya dan suhu dalam proses fotosintesis memberi pengaruh bagi kestabilan dan ketidakstabilan karotenoid dan klorofil. Penelitian ini bertujuan untuk mengetahui aktivitas fotostabilitas dan termostabilita...
Conference Paper
Full-text available
Information on mangrove species plays crucial role for sustainable management of coastal ecosystems. However, the current in-depth data acquisition for sustainable management of coastal ecosystems is still collected manually. The increased demand to obtain mangrove environmental data in a short time and at affordable cost has encouraged our researc...
Conference Paper
Currently a combination of fluorescence microscopy with spectroscopic analysis in a balance way has played an important role in providing new and exciting information for the in vivo study of cell, such as diatoms, photosynthetic marine microorganisms. Diatoms contain photosynthetic pigments, carotenoid and chlorophylls molecules, in their machiner...
Article
Full-text available
Erythrobacter flavus strain KJ5 (formerly called Erythrobacter sp. strain KJ5) is a yellowish marine bacterium that was isolated from a hard coral in the Karimunjawa Islands of Indonesia. Here, we report the complete genome sequence of the bacterium and provide a useful resource for studies of the biosynthetic pathways of its unique carotenoids.
Conference Paper
Full-text available
Determination of photosynthetic pigments in intact leaves is an essential step in the plant analysis. Along with the rapid development of digital imaging technology and artificial intelligence, now determination of plant pigments can be done in a non- destructive and real-time manner. In previous research, a prototype of the non-destructive and rea...
Article
Full-text available
Vivid orange petals of marigold have been studied well as the richest source of lutein. Although it is very promising to be explored as functional food ingredients, there were only few studies that described extraction methods which meet food grade requirements. Here, lutein was extracted from marigold petals using vegetable oil, subsequently encap...
Article
Full-text available
Karotenoid adalah pigmen yang memberikan warna kuning, jingga hingga merah. Karotenoid merupakan pigmen pendamping klorofil atau zat hijau daun yang menjalankan fungsi penyerapan energi cahaya untuk fotosintesis. Sumber karotenoid utama adalah tumbuhan, yang selanjutnya dikonsumsi dan dimetabolisme atau terakumulasi dalam tubuh hewan. Terdapat lebi...
Article
Full-text available
Two seaweed species – Kappaphycus alvarezii (Rhodophyta) and Padina australis (Phaeophyta) – have been commercially viable raw materials for the food industry. Despite their usefulness as sources of carrageenan and alginate, there is little information concerning their chlorophylls and carotenoids. Composition and quantification of the chlorophylls...
Article
Full-text available
Objective: The objective of this research was to investigate the antihypercholesterolemic activity of herbal medicine which consisted of pigment-rich simplicia from higher and lower plants, as well as to determine its pigments composition.Methods: Sprague-Dawley male rats were randomly divided into five cholesterol-rich diet groups as well as one n...
Article
Full-text available
Composition of pigments from four species of brown seaweeds (Phaeophyceae) collected from Panjang Island, Central Java, Indonesia, was investigated with spectroscopic method and reverse-phase high-performance liquid chromatography (RP-HPLC). Identification of pigments was based on their spectral and chromatographic properties and also confirmed by...
Article
Full-text available
Eosin Y telah berhasil diembankan pada Metal-Organic Framework Zirkonium Naftalendikarboksilat (MOF Zr-NDC) dengan cara menambahkan Eosin Y dalam pelarut etanol (0,034 ml, 10-4 M) pada MOF Zr-NDC (0,3 g). Pengukuran Powder X-Ray Diffraction (PXRD) dilakukan untuk mengkonfirmasi pembentukan MOF Zr-NDC dengan puncak khas pada 2θ di 6.47 dan 7.45. Spe...
Conference Paper
Full-text available
Separation and composition of carotenoids from Erythrobacter flavus are reported. E. flavus is a yellow aerobic marine bacterium. It was isolated from soft-coral Acropora nasuta. E. flavus was cultured in Shioi medium at 28C for 3 days. The cells of E. flavus from each growth phase were extracted with a mixture of methanol and acetone (7:3, v /v)....