Tanya Copley

Centre de Recherche sur les Grains Inc. (CÉROM) | CEROM · Plant Genetic Improvement

Deciphering the gene regulatory networks of critical quantitative trait loci associated with early maturity provides information for breeders to unlock soybean’s (Glycine max (L.) Merr.) northern potential and expand its cultivation range. The E8-r3 locus is a genomic region regulating the number of days to maturity under constant short-day photope...

‘AAC Choo’ is a spring, two-row, general purpose barley (Hordeum vulgare L.) released by the Ottawa Research and Development Centre, Agriculture and Agri-Food Canada. AAC Choo is high in yield, good lodging resistance, and moderately susceptible to Fusarium head blight (caused by Fusarium graminearum Schwabe). AAC Choo is recommended for commercial...

Plant transformation remains a major bottleneck to the improvement of plant science, both on fundamental and practical levels. The recalcitrant nature of most commercial and minor crops to genetic transformation slows scientific progress for a large range of crops that are essential for food security on a global scale. Over the years, novel stable...

In Canada, soybean (Glycine max (L.) Merr.) is primarily cultivated in three provinces (Ontario, Quebec, and Manitoba). Canadian breeders want to expand the current cultivation range to more northern agro-environments by developing early-maturing elite lines while maintaining good seed quality traits. To examine quantitative trait loci involved in...

Soybean [Glycine max (L.) Merr.] is a short-day crop for which breeders want to expand the cultivation range to more northern agro-environments by introgressing alleles involved in early reproductive traits. To do so, we investigated quantitative trait loci (QTL) and expression quantitative trait loci (eQTL) regions comprised within the E8 locus, a...

“AAC Sorel” is a spring, two-row, general-purpose barley (Hordeum vulgare L.) released by the Ottawa Research and Development Centre, Agriculture and Agri-Food Canada. AAC Sorel is similar in yield to the check cultivars with very good lodging resistance and moderately susceptible to Fusarium head blight (caused by Fusarium graminearum Schwabe). AA...

The SoyaGen project was a collaborative endeavor involving Canadian soybean researchers and breeders from academia and the private sector as well as international collaborators. Its aims were to develop genomics-derived solutions to real-world challenges faced by breeders. Based on the needs expressed by the stakeholders, the research efforts were...

Background: To continue to meet the increasing demands of soybean worldwide, it is crucial to identify key genes regulating flowering and maturity to expand the cultivated regions into short season areas. Although four soybean genes have been successfully utilized in early maturity breeding programs, new genes governing maturity are continuously b...

Biotic stress, as a result of plant-pathogen interactions, induces the accumulation of reactive oxygen species in the cells, causing severe oxidative damage to plants and pathogens. To overcome this damage, both the host and pathogen have developed antioxidant systems to quench excess ROS and keep ROS production and scavenging systems under control...

Principle component analysis score plots (PC1/PC2) for the effect of 11 antioxidant genes relative transcripts abundance on control, necrotic lesions and surrounding areas of necrotic lesions of Pathosystem I (A), II (B) and III (C). The ellipse represents the Hotelling T2 at a 95% confidence interval. Three biological replications were performed p...

Rhizoctonia solani Kühn infects most plant families and can cause significant agricultural yield losses worldwide; however, plant resistance to this disease is rare and short-lived, and therefore poorly understood, resulting in the use of chemical pesticides for its control. Understanding the functional responses of this pathogen during host infect...

KEGG pathway E.C. numbers associated with genes expressed at onset of necrosis. (XLSX)

Top 20 up-regulated genes of Rhizoctonia solani at onset and 24 hours post-onset of necrosis during soybean interactions. (DOCX)

Top 20 down-regulated genes of Rhizoctonia solani at onset and 24 hours post-onset of necrosis during soybean interactions. (DOCX)

qRT-PCR primers and thermocycling conditions. (DOCX)

Differentially expressed genes during Rhizoctonia-soybean interactions 24 hours post-onset of necrosis. (XLSX)

Differential gene expression between R. solani genes at onset and 24 h.p.o. of necrosis. (XLSX)

Summary of RNA-seq read numbers and alignment rates. (DOCX)

Differentially expressed genes during Rhizoctonia-soybean interactions at onset of necrosis. (XLSX)

KEGG pathway E.C. numbers associated with genes expressed 24 h.p.o. of necrosis. (XLSX)

KEGG pathway annotations that were unique for each Rhizoctonia solani-soybean interaction time point. (DOCX)

Background Rhizoctonia solani AG1-IA is a devastating phytopathogen causing Rhizoctonia foliar blight (RFB) of soybean worldwide with yield losses reaching 60%. Plant defense mechanisms are complex and information from different metabolic pathways is required to thoroughly understand plant defense regulation and function. Combining information from...

Application of biochar, a pyrolyzed biomass from organic sources, to agricultural soils is considered a promising strategy to sustain soil fertility leading to increased plant productivity. It is also known that applications of biochar to soilless potting substrates and to soil increases resistance of plants against diseases, but also bear the pote...

Many studies have investigated the effect of biochar on plant yield, nutrient uptake and soil microbial populations, however little work has been done on its effect on soilborne plant diseases. To determine the effect of maple bark biochar on Rhizoctonia damping-off, eleven plant species were grown in a soilless potting substrate amended with diffe...

Plant responses to insect herbivory are regulated through complex, hormone-mediated interactions. Some caterpillar species have evolved strategies to manipulate this system by inducing specific pathways that suppress plant defense responses. Effectors in the labial saliva (LS) secretions of Spodoptera exigua caterpillars are believed to induce the...

Current sampling methods for identification of honeybee microsporidians (Nosema apis and Nosema ceranae) involve killing adult honeybees. The current study monitored the presence of N. apis and N. ceranae in honeybee frass and bottom scraps collected from six hives located in Deschambault, Quebec, Canada, from 2009 to 2010. Infection rates of N. ce...

Nosemosis is a disease of adult honey bees, Apis mellifera Linnaeus (Hymenoptera: Apidae), caused by two described species of Microsporidia: Nosema ceranae Fries and Nosema apis Zander. The epidemiology of N. apis is well understood; however, little is known about N. ceranae in Canadian apiaries. The following study aimed to determine the seasonal...

Here, we are presenting a gas chromatography-mass spectrometry (GC/MS) approach for the study of infection of the worker honey bee (Apis mellifera L.) by the newly emerged obligate intracellular parasite Nosema ceranae based on metabolite profiling of hemolymph. Because of the severity of the disease, early detection is crucial for its efficient co...

To determine whether Nosema ceranae and Nosema apis are present in different gland tissues of honeybee, Apis mellifera L. and to monitor spore presence and quantity in these glands in naturally infected hives from July 2009 to July 2010 in Quebec, Canada. Nosema spp. were quantified using duplex quantitative real-time PCR in the thoracic salivary,...