Tanja Gödecke

• PhD PharmD
• University of Illinois Chicago

The ¹H NMR spectra of crude extracts from a total of 33 Actaea samples were acquired and analyzed for their species- and plant part-specific metabolomic characteristics by identifying fingerprint resonances via visual observation as well as a chemometric approach using principal component analysis (PCA). The main study subjects were the roots/rhizo...

The cycloartane triterpene content in the roots/rhizomes (RR) and aerial parts (PX) of Actaea racemosa (AR), A. podocarpa (AP), and A. cordifolia (AC) have been investigated by quantitative 1H NMR (qHNMR). Thereby, it was demonstrated that qHNMR represents a powerful methodology for the analysis of crude plant extracts as it does not rely on the ra...

Page SI-9 of Supporting Information file jm500734a_si_001.pdf. The correct molecular weight of the target compound (MWt) is 290.27 g/mol, rather than 209.29 g/mol, and is reflected in the Supporting Information for this Addition and Correction.

Three (9βH)-pimaranes, 1, 2, and 3, and two (9βH)-17-norpimaranes, 4 and 5, belonging to a rare compound class in nature, were obtained from the tubers of Icacina trichantha for the first time. Compound 1 is a new natural product, and 2-5 have been previously reported. The structures were elucidated based on NMR and MS data, and optical rotation va...

In any biomedical and chemical context, a truthful description of chemical constitution requires coverage of both structure and purity. This qualification affects all drug molecules, regardless of development stage (early discovery to approved drug) and source (natural product or synthetic). Purity assessment is particularly critical in discovery p...

Further to our previous report on the isolation and X-ray crystallographic structures of two 9βH-pimaranes (icacinol 2 and humirianthol 3) from the tubers of the African medicinal plant Icacina trichantha, compounds 1, 4 and 5 were obtained. Compound 1 was elucidated to be a new 17-hydroxyl derivative of icacinol. The absolute configuration was est...

The present study demonstrates the importance of adequate precision when reporting the delta and J parameters of frequency domain H-1 NMR (HNMR) data. Using a variety of structural dasses (terpenoids, phenolics, alkaloids) from different taxa (plants, cyanobacteria), this study develops rationales that explain the importance of enhanced precision i...

A method was developed to distinguish Vaccinium species based on leaf extracts using nuclear magnetic resonance spectroscopy. Reference spectra were measured on leaf extracts from several species, including lowbush blueberry (Vaccinium angustifolium), oval leaf huckleberry (Vaccinium ovalifolium), and cranberry (Vaccinium macrocarpon). Using princi...

The antimicrobial activity of lingonberry (Vaccinium vitis-idaea L.) was evaluated against two oral pathogens, Streptococcus mutans and Fucobacterium nucleatum. Long-bed gel permeation chromatography (GPC; Sephadex LH-20) yielded purified flavonoids, with the most efficient minimum inhibitory concentrations (MICs) against planktonic cells in the an...

Three new germacrane sesquiterpenes and a new m-menthane monoterpene were isolated together with four known compounds from the pericarp of Illicium lanceolatum, an adulterant to star anise (Illicium verum). All compounds were isolated from Illicium plants for the first time. The absolute stereochemistry of all germacranes and m-menthane was establi...

Introduction: Nuclear magnetic resonance (NMR) spectroscopy is increasingly employed in the quantitative analysis and quality control (QC) of natural products (NP) including botanical dietary supplements (BDS). The establishment of QC protocols based on quantitative (1) H NMR (qHNMR) requires method validation. Objective: Develop and validate a...

The fruits of Vaccinium vitis-idaea L. are a valuable source of biologically active flavonoid derivatives. For studies focused on the purification of its quercetin glycosides (QGs) and related glycosides from plants and for the purpose of biological studies, the availability of numeric datasets from computer-assisted 1H iterative full spin analysis...

Six germacrane sesquiterpenes (1 – 6) were isolated from Illicium lanceolatum, five of which (1 – 4, 6) are new structures. Their absolute configurations were established by a combination of modified mosher's method and NMR experiments. 4 and 5 showed proliferative activity on SH-SY5Y (a neuroblastoma cell line) by 36.18% (at 62.5µM) and 45.75% (at...

The development of analytical methods for parallel characterization of multiple phytoconstituents is essential to advance the quality control of herbal products. While chemical standardization is commonly carried out by targeted analysis using gas or liquid chromatography-based methods, more universal approaches based on quantitative (1)H NMR (qHNM...

Protostane triterpenes belong to a group of tetracyclic triterpene that exhibit unique structural characteristics. Their natural distribution is primarily limited to the genus Alisma of the Alismataceae family, but they have also been occasionally found in other plant genera such as Lobelia, Garcinia, and Leucas. To date, there are 59 known protost...

Nuclear Magnetic Resonance (NMR) spectroscopy is a highly versatile technique for structural elucidation in natural product research. However, its implementation as the ultimate identity test requires the accurate determination of the position and multiplicity of each proton resonance of the compound(s) of interest. In this report, quantitative ¹H...

From the seeds of Alisma orientale, cis-aconitic anhydride ethyl ester and cis-2,4,5-trihydroxycinnamic acid were isolated, together with nine known phenolic compounds and a megastigmane sesquiterpene. All compounds are reported for the first time from Alisma species.

While Sephadex LH-20 gel is frequently employed as a stationary phase during pre-separations and in open column chromatography systems, its separation power in long-bed gel permeation chromatography (GPC) applications is much less prevalent. Aimed at the characterization of bioactive constituents, a long-bed GPC protocol was established for lingonb...

Covering the literature from mid-2004 until the end of 2011, this review continues a previous literature overview on quantitative (1)H NMR (qHNMR) methodology and its applications in the analysis of natural products. Among the foremost advantages of qHNMR is its accurate function with external calibration, the lack of any requirement for identical...

Botanical dietary supplements and herbal remedies are widely used for health promotion and disease prevention. Due to the high chemical complexity of these natural products, it is essential to develop new analytical strategies to guarantee their quality and consistency. In particular, the precise characterization of multiple botanical markers remai...

Black cohosh preparations are popular dietary supplements among women seeking alternative treatments for menopausal complaints. For decades, triterpene glycosides and phenolic acids have dominated the phytochemical and biomedical research on this plant. In this study, we provide evidence that black cohosh contains an unexpected and highly diverse g...

Despite numerous in vitro/vivo and phytochemical studies, the active constituents of Angelica sinensis (AS) have not been conclusively identified for the standardization to bioactive markers. Phytochemical analyses of AS extracts and fractions that demonstrate activity in a panel of in vitro bioassays, have repeatedly pointed to ligustilide as bein...

Women who experience hot flashes as a side effect of tamoxifen (TAM) therapy often try botanical remedies such as black cohosh to alleviate these symptoms. Since pharmacological activity of TAM is dependent on the metabolic conversion into active metabolites by the action of cytochromes P450 2D6 (CYP2D6) and 3A4, the objective of this study was to...

Vaccinium spp. (Blueberries, Cranberries, and Bilberries) have been traditionally used by indigenous cultures and are linked to maintenance and low instances of diabetes onset [1]. The ability to quickly screen extracts for novel compounds, new sources or higher levels of known compounds, monitoring of „ripeness“, and source would greatly optimize...

Previously, the presence of a wide variety of chemically diverse steroids has been identified in both flora and fauna. Despite the relatively small differences in chemical structures and large differences in physiological function of steroids, new discoveries indicate that plants and animals are more closely related than previously thought. In this...

The selective estrogen modulator tamoxifen has been proven efficacious in the treatment and prevention of breast cancer. However, tamoxifen treatment leads to consequences of estrogen deprivation including inducing menopausal hot flashes. In addition, tamoxifen causes enhanced endometrial cell proliferation resulting in an increased endometrial can...

Earlier studies reported serotonergic activity for cimicifugic acids (CA) isolated from Cimicifuga racemosa. The discovery of strongly basic alkaloids, cimipronidines, from the active extract partition and evaluation of previously employed work-up procedures has led to the hypothesis of strong acid/base association in the extract. Re-isolation of t...

As an extension of work on the recently discovered nitrogenous metabolites from Cimicifuga/Actaea species, three new guanidine alkaloids have been isolated and characterized from C. racemosa (syn. A. racemosa) roots. Of these, cyclo-cimipronidine (1) and cimipronidine methyl ester (2) are congeners of cimipronidine (3), whereas dopargine (5) is a d...

Cimicifuga racemosa (L.) Nutt. (syn. Actaea racemosa L., black cohosh) is used to relieve menopausal hot flashes, although clinical studies have provided conflicting data, and the active constituent(s) and mechanism(s) of action remain unknown. Because serotonergic receptors and transporters are involved with thermoregulation, black cohosh and its...

In previous receptor binding studies using a comprehensive panel of 5-HT receptor subtypes with a 40% (v/v) isopropanolic, a 75% (v/v) ethanolic, and a methanolic crude extract of C. racemosa roots, the methanolic extract exhibited the highest potency in displacing the ligand from the receptor subtype 7 [1,2]. Subsequent screening studies were ther...

Structural examn. of the metabolic pool of the aerial parts of Pelargonium sidoides DC has led to the isolation of two new metabolites, 4-allyl-2,5-dimethoxyphenol-1-β-D-glucopyranoside and 6,7-dihydroxycoumarin-8-sulfate, along with a series of uncommon compds. including (2R,3R)-(+)-dihydroquercetin-3-β-glucopyranoside, (2R,3R)-(+)-dihydrokaempfer...