Tânia Nobre

Tânia Nobre
Universidade de Évora | uevora · Institute of Mediterranean Agricultural and Environmental Sciences (ICAAM)

PhD

About

150
Publications
34,705
Reads
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1,303
Citations
Citations since 2016
56 Research Items
994 Citations
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2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
Introduction
Research interests: - habitat-adapted interactions - co-evolution and speciation - evolutionary and ecological determinants of species distribution - population structure and life history evolution - organisms-environment interactions
Additional affiliations
July 2013 - July 2016
Universidade de Évora
Position
  • Invited Researcher
April 2013 - present
Universidade de Évora
Position
  • Understanding symbiotic diversity
May 2008 - October 2012
Wageningen University & Research
Position
  • Fungus-growing termites mutualism - a tripartite symbiosis

Publications

Publications (150)
Article
Symbiotic interactions between macrotermitine termites and their fungal symbionts have a moderate degree of specificity. Consistent with horizontal symbiont transmission, host switching has been frequent over evolutionary time so that single termite species can often be associated with several fungal symbionts. However, even in the few termite line...
Article
Full-text available
We investigate the scope for selection at the level of nuclei within fungal individuals (mycelia) of the mutualistic Termitomyces cultivated by fungus-growing termites. Whereas in most basidiomycete fungi the number and kind of nuclei is strictly regulated to be two per cell, in Termitomyces mycelia the number of nuclei per cell is highly variable....
Article
Full-text available
The concept of individuality has changed, since symbiosis is now accepted as being widespread and not an exception. Symbiotic microorganisms are not only crucial for the evolutionary and ecological success of many organisms (take land plants as an iconic example) but can also be key to many current human caused challenges (biomass degradation and b...
Article
Full-text available
The olive moth -Prays oleae Bern.- remains a significant pest of olive trees showing situation dependent changes in population densities and in severity of damages. The genetic variability of olive moth was assessed on three main olive orchards regions in Portugal by three different markers (COI, nad5 and RpS5), suggesting high species diversity al...
Article
Full-text available
The applied importance of symbiosis has been gaining recognition. The relevance of symbiosis has been increasing in agriculture, in developing sustainable practices, including pest management. Insect symbiotic microorganisms’ taxonomical and functional diversity is high, and so is the potential of manipulation of these microbial partners in suppres...
Article
Full-text available
The current symbiotic view of the organisms also calls for new approaches in the way we perceive and manage our pest species. The olive fruit fly, the most important olive tree pest, is dependent on an obligate bacterial symbiont to its larvae development in the immature fruit. This symbiont, Candidatus (Ca.) Erwinia dacicola, is prevalent througho...
Article
Full-text available
Over the past few decades, species distribution modelling has been increasingly used to monitor invasive species. Studies herein propose to use Cellular Automata (CA), not only to model the distribution of a potentially invasive species but also to infer the potential of the method in risk prediction of Reticulitermes grassei infestation. The test...
Article
Full-text available
Developing below the soil surface desert, truffles are hard to find. Within Terfezia genus, at least 18 species are described and many are endemic to the Mediterranean basin. Ecological and geographic information are key factors for species diagnosis, and so far Terfezia species are believed to be linked to either acidic or basic soils or to specif...
Article
Full-text available
The olive fruit fly, specialized to become monophagous during several life stages, remains the most important olive tree pest with high direct production losses, but also affecting the quality, composition, and inherent properties of the olives. Thought to have originated in Africa is nowadays present wherever olive groves are grown. The olive frui...
Article
Symbiotic-based pest management strategies are promising but require previous knowledge on the microbial community structure and on key microorganisms. Highly specialised pests, as the monophagous olive moth, are likely to have co-evolved microbiota that aid in overcoming specific plant defences that have emerged from the tight insect-plant interac...
Article
The European grapevine moth, Lobesia botrana , has high economic impact on grapevines being one of the most harmful pests of vineyards worldwide. Wolbachia infection has not yet been reported for this moth. We systematically look for Wolbachia presence in L. botrana from three areas within the viticultural region of Alentejo (Portugal), via wsp gen...
Article
Full-text available
Worldwide sustainable development is threatened by current agricultural land change trends, particularly by the increasing rural farmland abandonment and agricultural intensification phenomena. In Mediterranean countries, these processes are affecting especially traditional olive groves with enormous socioeconomic costs to rural areas, endangering...
Technical Report
Full-text available
19 experts from the five main EU olive producing countries were brought together in a Focus Group to explore more sustainable farming practices, including the use of non-chemical pesticides to tackle the main diseases and pests threatening EU olive production. The Focus Group worked together for around 12 months and met face-to-face twice to carry...
Article
Full-text available
Prays oleae is the second most important pest in Mediterranean olive groves, causing substantial damage on olive production. We used mitochondrial [cytochrome c oxidase subunit I (COI), and NADH dehydrogenase subunit 5 (nad5)] and nuclear [ribosomal protein S5 (RpS5)] amplicons to assess the population variability in five main olive producing regio...
Article
Full-text available
Symbioses with soil microorganisms are central in shaping the diversity and productivity of land plants and provide protection against a diversity of stresses, including metal toxicity. Arbuscular mycorrhizal fungi (AMF) can form extensive extraradical mycelial networks (ERM), which are very efficient in colonizing a new host. We quantified the res...
Article
Full-text available
In this study, the presence and variability of Colletotrichum spp. was evaluated by comparing fungal isolates obtained from olive trees under long-time phytosanitary treatments with trees without any phytosanitary treatments (treated and untreated, respectively). Olive fruits of trees of the highly susceptible ‘Galega vulgar’ cultivar growing in th...
Article
Full-text available
The management of the olive fruit fly (Bactrocera oleae, Dacus oleae) is traditionally based upon the use of organophosphate insecticides, mainly dimethoate. In this evolutionary arms race between man and pest, the flies have adapted a pesticide resistance, implying two point-mutations of the Ace gene-I214V and G488S-and a 9bp deletion-∆3Q. We revi...
Article
Desert truffles (mycorrhizal hypogeous Ascomycota) are found in arid and semi-arid areas of the globe and have great ecological and economic importance. Terfezia is undoubtedly the most diversified of all desert truffle genera, but its taxonomy is far from resolved. Specifically, the large number of newly described species plus the high intraspecif...
Article
Full-text available
The symbiosis established between arbuscular mycorrhizal fungi (AMF) and roots of most land plants plays a key role in plant nutrient acquisition and alleviation of environmental stresses. Despite the ubiquity of the symbiosis, AMF and host species display significant specificity in their interactions. To clarify preferential associations between w...
Data
Phylogenetic relationship between Prays oleae and other Prays species with data available on GenBank (accession code given on tree), based on the COI amplicon, by Maximum Likelihood method. (PDF)
Data
Variables analysed, including bioclimatic and land cover derived maps. (PDF)
Data
Sampled locations and geographical coordinates. Locations with alphabetic code only were sampled by local associations and/or by the Regional Directorates for Agriculture. (PDF)
Data
Compressed folder containing 4 data files: Fasta file with Prays sequences alignment, Newick files of S1 and S2 Figs and the BEAST xml constrained and unconstrained files. (ZIP)
Data
Evolutionary relationships between Prays oleae and other Prays species with data available on GenBank (accession code given on tree), based on the COI amplicon, by Neighbor-Joining. (PDF)
Data
TCS haplotype networks of sampled specimens, considering a) the mitochondrial marker COI, b) the mitochondrial marker nad5, and c) the nuclear marker RpS5. Each circle represents a sequence; the size of the circle is proportional to number of individuals with a particular haplotype sequence. (PDF)
Data
GenBank accession numbers of the sequences obtained in this study. Code corresponds to the location code as in Table 1 followed by specimen number. (PDF)
Article
Full-text available
Changes in flagellate protist communities of subterranean termite Reticulitermes grassei across different locations were evaluated following four predictions: (i) Rural endemic (Portugal mainland) termite populations will exhibit high diversity of symbionts; (ii) invasive urban populations (Horta city, Faial island, Azores), on the contrary, will e...
Article
Full-text available
To degrade lignocellulose efficiently, lower termites rely on their digestive tract's specific features (i.e., physiological properties and enzymes) and on the network of symbiotic fauna harboured in their hindgut. This complex ecosystem, has different levels of symbiosis, and is a result of diverse co-evolutionary events and the singular social be...
Article
In traditional plant breeding, selected elite breeding lines play an important role as a source for building-up and improving breeding populations. Currently, the focus for using wild relatives together with landraces in breeding programs is gaining more attention in the context of global climate changes. Differences in plant growth adaptation unde...
Article
Carrot is an outcrossing species and levels of gene flow between populations, and even between wild and domesticated relatives, are expected to be high. Cases of natural hybridization and introgression of crops and wild relatives have been reported. Have these events diluted any putative habitat-adapted genotypes? In other words, can we still find...
Article
Full-text available
By definition, the domestication process leads to an overall reduction of crop genetic diversity. This lead to the current search of genomic regions in wild crop relatives (CWR), an important task for modern carrot breeding. Nowadays massive sequencing possibilities can allow for discovery of novel genetic resources in wild populations, but this qu...
Data
Reconstructed phylogeny based on a AOX1 fragment. The phylogeny corresponds to the majority rule consensus tree of trees sampled in a Bayesian analysis. Only fragments in exons were considered. Arabidopsis thaliana was used as outgroup. The numbers above the branches refer to the Bayesian posterior probability of the nodes (more than 50%) derived f...
Data
Reconstructed phylogeny based on a AOX1 fragment. The phylogeny corresponds to the majority rule consensus tree of trees sampled in a Bayesian analysis. Only intron 1 was considered. Arabidopsis thaliana was used as outgroup. The numbers above the branches refer to the Bayesian posterior probability of the nodes (more than 50%) derived from 19500 M...
Data
Reconstructed phylogeny based on a AOX1 fragment. The phylogeny corresponds to the majority rule consensus tree of trees sampled in a Bayesian analysis. Two insertions at the intron were removed. Arabidopsis thaliana was used as outgroup. The numbers above the branches refer to the Bayesian posterior probability of the nodes (more than 50%) derived...
Data
Sample locations, geographic coordinates, populations and individual plants codes. (PDF)
Data
Intron 1 insertions and homologies according to NCBI. (PDF)
Article
Full-text available
Incorrectly or simply not annotated data is largely increasing in most public databases, undoubtedly caused by the rise in sequence data and the more recent boom of genomic projects. Molecular biologists and bioinformaticists should join efforts to tackle this issue. Practical challenges have been experienced when studying the alternative oxidase (...
Article
Full-text available
Based on molecular dating, the origin of insect agriculture is hypothesized to have taken place independently in three clades of fungus-farming insects: the termites, ants or ambrosia beetles during the Paleogene (66-24 Ma). Yet, definitive fossil evidence of fungus-growing behavior has been elusive, with no unequivocal records prior to the late Mi...
Conference Paper
The flagellate protist communities are an important part of the termite, as they lead the lignocellulose digestion. Termites (Reticulitermes grassei) were sampled from forest and urban environments in mainland Portugal where they are native and in Faial Island, Azores (invasive populations). Termites’ gut contents was analysed morphologically and t...
Article
Full-text available
Stress-adaptive cell plasticity in target tissues and cells for plant biomass growth is important for yield stability. In vitro systems with reproducible cell plasticity can help to identify relevant metabolic and molecular events during early cell reprogramming. In carrot, regulation of the central root meristem is a critical target for yield-dete...
Data
(A) Growth curve of primary cultures system of D. carota L. cv. Rotin during 28 days in culture at 21°C in four individual plants. Data are shown as callus FW values and represented as mean ± SD. (B) Explants from the secondary phloem of carrot tap roots PCS. Aspect of the explants (1) before (T0), (2) 14 days, and (3) 28 days after in vitro inocul...
Article
Full-text available
Arbuscular mycorrhizal fungi (AMF) are root-inhabiting fungi that form mutualistic symbioses with their host plants. AMF symbiosis improves nutrient uptake and buffers the plant against a diversity of stresses. Rhizophagus irregularis is one of the most widespread AMF species in the world, and its application in agricultural systems for yield impro...
Article
Full-text available
The alternative oxidase (AOX) is a key enzyme of the alternative respiration pathway and is involved in plant response to a variety of environmental stresses. The genes encoding for this enzyme are highly polymorphic and thus likely good candidates for molecular marker development to assist plant breeding on stress tolerance. In carrot, DcAOX1 gene...
Article
Full-text available
Molecular plant breeding usually overlooks the genetic variability that arises from the association of plants with endophytic microorganisms, when looking at agronomic interesting target traits. This source of variability can have crucial effects on the functionality of the organism considered as a whole (the holobiont), and therefore can be select...
Article
Full-text available
Significance Old World (sub)tropical fungus-growing termites owe their massive ecological footprints to an advanced symbiosis with Termitomyces fungi. They also have abundant gut bacteria, but the complementarity roles of these symbionts have remained unclear. We analyzed the genomic potential for biomass decomposition in a farming termite, its fun...
Article
Gut microbes play a crucial role in decomposing lignocellulose to fuel termite societies, with protists in the lower termites and prokaryotes in the higher termites providing these services. However, a single basal subfamily of the higher termites, the Macrotermitinae, also domesticated a plant biomass-degrading fungus (Termitomyces), and how this...
Article
Full-text available
We present a new perspective for the role of Termitomyces fungi in the mutualism with fungus-growing termites. According to the predominant view, this mutualism is as an example of agriculture with termites as farmers of a domesticated fungus crop, which is used for degradation of plant-material and production of fungal biomass. However, a detailed...
Chapter
Full-text available
Biodiversity is the variety of nature in terms of the abundance and distributions of, and interactions between genotypes, species, communities, and eco-systems (Ash et al . 2009). Ecosystem services are the benefi ts people derive from nature. If we want to establish the links between the two (if any, and causal or not), we have to recognize that b...
Article
Full-text available
In fungus-growing termites, fungi of the subgenus Pseudoxylaria threaten colony health through substrate competition with the termite fungus (Termitomyces). The potential mechanisms with which termites suppress Pseudoxylaria have remained unknown. Here we explore if Actinobacteria potentially play a role as defensive symbionts against Pseudoxylaria...
Article
The mutualistic symbiosis between fungus-growing termites and Termitomyces fungi originated in Africa and shows a moderate degree of interaction specificity. Here we estimate the age of the mutualism and test the hypothesis that the major splits have occurred simultaneously in the host and in the symbiont. We present a scenario where fungus-growing...