Tânia R. Dias

Tânia R. Dias
University of Aveiro | UA · Department of Biology

PhD in Biomedicine

About

91
Publications
52,491
Reads
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1,243
Citations
Introduction
Tania R. Dias completed her Ph.D. in Biomedicine in 2019, at the Faculty of Health Sciences - University of Beira Interior (FCS-UBI), in collaboration with the Institute of Biomedical Sciences Abel Salazar - University of Porto (ICBAS-UP), funded by the "Fundação para a Ciência e Tecnologia". In 2017-2018, she was awarded a Fulbright fellowship to join the Andrology lab, at the Cleveland Clinic, Cleveland, Ohio, USA, for 8 months, as a visiting researcher.
Additional affiliations
April 2020 - March 2021
University of Aveiro
Position
  • Researcher
Education
September 2012 - October 2019
Universidade da Beira Interior
Field of study
  • Biomedicine
September 2010 - July 2012
Universidade da Beira Interior
Field of study
  • Biomedical Sciences
September 2007 - July 2010
Universidade da Beira Interior
Field of study
  • Biomedical sciences

Publications

Publications (91)
Article
Full-text available
In the search for a better and brighter future, the use of natural luminescent renewable materials as substitutes for synthetic ones in the energy field is of prime importance. The incorporation of natural pigments (e.g., xanthophylls and phycobiliproteins) is a fundamental step in a broad spectrum of applications that are presently marred by their...
Article
BACKGROUND Natural products are under the spotlight due to the increasing awareness of consumers. Among these products is phycocyanin (C-PC), a blue colorant that can be extracted from cyanobacteria - a poorly explored feedstock - and applied in several fields, due to its argued health benefits. Arthropira (previously called Spirulina) is nowadays...
Chapter
Reactive oxygen species (ROS) are continuously produced in semen and are essential for important spermatozoa functions that allow fertilization. However, an excessive amount of ROS is associated with poor sperm quality, which can compromise male fertility potential. This chemiluminescence assay is based on the production of light through the reacti...
Article
Full-text available
Testicular germ cell tumors (TGCTs) are predominant in young males (15–44 years). Seminomatous and non-seminomatous TGCTs account for about 98% of all TGCTs cases. In this study, we aimed to compare the sperm proteome of patients with seminomatous and non-seminomatous TGCTs to identify possible protein biomarkers that could help distinguish between...
Article
Significance: Antioxidants are essential for the maintenance of cellular redox homeodynamics in the male reproductive tract, playing a key role in fertilizing potential. Reactive oxygen species (ROS), at physiological levels, are essential for sperm function and fertilization. Under pathological conditions, abnormal production of ROS may occur. Re...
Article
Full-text available
Lactate is a key metabolite for the normal occurrence of spermatogenesis. In the testis, lactate is produced by the Sertoli cells and transported to germline cells. Monocarboxylate transporters (MCTs) are key players in that process. Among the family of MCTs, MCT1 is at least partly responsible for lactate uptake by the germ cells. We aimed to perf...
Poster
Full-text available
Monocarboxylate transporters (MCTs) are important lactate transporters expressed in mammalian cells. In testis, the lactate is produced by the Sertoli cells and transported to germline cells. It has been teported that MCTs play a pivotal role in testis energy metabolism, essentially MCT4, but nothing is known about the role of MCT1 in testis and ma...
Poster
Full-text available
Insulin-degrading enzyme (IDE), also known as insulysin, is a zinc metalloprotease responsible for insulin degradation. Therefore, Ide knockout (KO) mice present increased insulin levels. It is known that insulin dysregulation has a major impact in male fertility. Yet, the specific role of IDE in male reproductive function remains to be unveiled. H...
Article
Despite conflicting data on doxorubicin (DOX) reproductive toxicity, its chemotherapeutic potential sustains its use to treat different types of cancer. This work was designed to study the protective effect of a newly synthesized thiocyanoacetamide (TA), in comparison with selenium (Se), against doxorubicin-induced in vitro toxicity in rat Sertoli...
Preprint
Full-text available
Mitochondrial respiratory states and rates MitoEAGLE Task Group
Chapter
Late child-bearing and global low fertility rates led to an increasing number of couples seeking medical counseling and assistance to achieve conception. The specific evaluation of male partners is pivotal in the investigation of infertile couples. Semen assessment represents the cornerstone in the evaluation of the fertility potential of the male...
Chapter
Conventional approaches of semen assessment are the basis of male fertility evaluation. However, its clinical value has been questioned due to the poor correlation with reproductive outcomes. Results from the analysis of standard parameters such as motility, concentration, and morphology often lead to the misclassification of fertility status. Tech...
Article
Purpose: L-Theanine is the major free amino acid present in tea (Camellia sinensis L.). The effects of several tea constituents on male reproduction have been investigated, but L-theanine has been overlooked. Sertoli cells (SCs) are essential for the physical and nutritional support of germ cells. In this study, we aimed to investigate the ability...
Article
Semen contains leucocytes and round cells, besides spermatozoa. The objective of this study was to identify whether the proteins from round cells and leucocytes affect the proteomic analysis of spermatozoa. Cryopreserved human sperm samples were divided into four groups: (1) samples with ≥1 × 10^6/ml leucocytes unprocessed; (2) samples with ≥1 × 10...
Article
Full-text available
Insulin-degrading enzyme (IDE) degrades and inactivates bioactive peptides such as insulin. As insulin is a master regulator of glucose homeostasis, lack of IDE is expected to have a profound impact on both insulin and glucose levels. This article shares data on glucose and insulin homeostasis of control, heterozygous and knockout mice for Ide afte...
Article
Full-text available
Purpose: Aberrant expression of seminal plasma proteins are associated with altered homeostasis that may affect the fertilizing ability of spermatozoa. However, the precise roles of seminal exosomes on sperm function remain unclear. The objective of this study was to identify the differentially expressed proteins (DEPs) associated with varicocele-...
Preprint
Full-text available
As the knowledge base and importance of mitochondrial physiology to human health expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unif...
Article
Full-text available
The process that allows cells to control their pH and bicarbonate levels is essential for ionic and metabolic equilibrium. Carbonic anhydrases (CAs) catalyse the conversion of CO2 to HCO 3 - and H+ and are thus essential for this process. Herein, we inhibited CAs with acetazolamide - ACT and SLC-0111 - to study their involvement in the metabolis...
Article
Insulin-degrading enzyme (IDE) is a zinc metalloprotease responsible for degrading and inactivating several bioactive peptides, including insulin. Individuals without this enzyme or with a loss-of-function mutation in the gene that codifies it, present hyperinsulinemia. In addition, impairment of IDE-mediated insulin clearance is associated with th...
Article
Full-text available
In sperm proteomic experiments round cells and leukocyte proteins are profiled along with sperm proteome. The influence of round cell and leukocyte proteins on the sperm proteome has not been investigated. The objective of this study was to identify if the proteins from round cells, including leukocytes, interfere with the proteomic analysis of spe...
Article
Full-text available
Elevated levels of reactive oxygen species (ROS) are a major cause of male infertility. However, some men with high seminal ROS levels are still fertile. The main objective of this study was to understand the molecular mechanism(s) responsible for the preservation of fertility in those men. Semen samples from fertile men were divided into two group...
Article
Full-text available
Testicular cancer seminoma is one of the most common types of cancer among men of reproductive age. Patients with this condition usually present reduced semen quality, even before initiating cancer therapy. However, the underlying mechanisms by which testicular cancer seminoma affects male fertility are largely unknown. The aim of this study was to...
Article
In human sperm proteomic experiments, leukocyte and round cell proteins may contaminate the sperm proteome and affects the bioinformatic results. The main objective of this study was to identify the possible interference of these proteins, especially from leukocytes, in identification of sperm functional pathways through proteomic and bioinformatic...
Article
Full-text available
Purpose: Patients with non-seminoma testicular cancer (NSTC) cancer can be subfertile or infertile, and present reduced sperm quality, but the underlying mechanisms are unknown. The aim of this study was to compare the sperm proteome of patients with NSTC, who cryopreserved their sperm before starting cancer treatment, with that from healthy fertil...
Chapter
White tea (WT) is one of the rarest, least produced, and expensive types of tea, which has only gained attention in the last decade. The WT manufacturing process, that is, Camellia sinensis fresh leaves/buds being dried, is minimal compared to production processes used for other types of tea. For that reason, WT retains a high amount of its polyphe...
Article
Caffeine is one of the most worldwide consumed methylxanthines. It is well-known for its thermogenic and cell metabolism modulating effects. Based on methylxanthines' chemical structure, 8-(3-phenylpropyl)-1,3,7-triethylxanthine (PTX) is a novel adenosine antagonist with higher receptor affinity than caffeine. Therefore, we hypothesized that PTX me...
Article
Full-text available
Background: The 'omics' approach for a noninvasive diagnosis of male reproductive system disorders has gained momentum during the last decade, particularly from a screening and prognosis point of view. Due to the rapid development in assisted reproductive technologies (ART) over the years, the major focus of proteomic studies has been around the e...
Article
Obesity is one of the most challenging health issue worldwide that has been steadily increasing in the last decades. Obesity arises from a positive energy balance in result of increased intake and decreased energy expenditure, usually due to inadequate dietary habits and a sedentary lifestyle, which results in the accumulation of excess body fat. T...
Article
Background: The incidence of diabetes mellitus (DM) is reaching alarming proportions worldwide, particularly because it is increasingly affecting younger people. This reflects the sedentary lifestyle and inappropriate dietary habits, especially due to the advent of processed foods in modern societies. Thus, unsurprisingly, the first medical recomm...
Poster
Full-text available
Obesity has been increasing in the last decades and is one of the most prolific health issues worldwide. It can be mediated by several factors, including an excessive amount of body fat that may cause a deleterious impact on human health. There are few pharmacological tools to address obesity, being low-calorie diet and physical activity the main r...
Chapter
The spermatozoon is a highly specialized cell that is formed through a complex cellular program of differentiation during spermatogenesis. It has a unique structure and chromatin that reflects its vital function. Morphologically it comprises a head, a midpiece and a tail. The sperm DNA is confined to the nucleus of the head and it has a characteris...
Chapter
The epididymis is a long convoluted organ of the male reproductive tract. Its functionality has been overlooked for many years, but it is currently accepted that it has a preponderant role in spermatozoa post-testicular maturation. The epididymis presents a high secretory activity. Several proteins can be released in bulk through apical blebs or ca...
Article
Sertoli cells are crucial for the success of spermatogenesis, which is the biological process that ensures male fertility. These cells present high metabolic rates, being often subjected to high oxidative stress levels that, if uncontrolled, may compromise male fertility. Since the most abundant tea catechin, epigallocatechin-3-gallate (EGCG), has...
Poster
Full-text available
Background: Sertoli cells possess a unique metabolism that functions at a high rate to maintain spermatogenesis. Thus, they are often subjected to high oxidative stress levels, which need to be controlled to avoid subfertility/infertility outcomes. Materials and methods: In this study we evaluated the effects of epigallocatechin gallate (EGCG) on t...
Poster
Full-text available
Background: Dehydroepiandrosterone (DHEA) is a precursor of androgens that acts through its metabolites, one of which is 7-oxo-dehydroepiandrosterone (7-oxo-DHEA). Sertoli cells (SCs) are responsible for the support of spermatogenesis, having metabolic characteristics modulated by androgens. Disruption in androgen synthesis may compromise SCs funct...
Poster
Full-text available
Introduction: Pre-diabetes is a major risk factor for the development of type 2 diabetes mellitus. This prodromal stage encompasses some, but not all, of type 2 diabetes mellitus diagnostic criteria. Pre-diabetes has been recently associated with altered testicular function and increased testicular oxidative stress. Tea is widely consumed and its a...
Presentation
Full-text available
Diabetes mellitus (DM) is one of the greatest threats to human health and the number of diabetic individuals is rapidly increasing. Prediabetes is a reversible prodromal stage of type 2 DM (T2DM) which encompasses some, but not all, T2DM characteristics. The brain, particularly the cortex, is very susceptible to hyperglycaemia and oxidative stress...
Poster
Full-text available
Introduction: Type 2 diabetes mellitus (T2DM) is one of the most prevalent and serious metabolic diseases affecting young males on modern societies. This pathological condition is closely related to current lifestyle habits and induces metabolic alterations particularly in glucose metabolism, which is vital for the normal occurrence of spermatogene...
Presentation
Introduction: Dehydroepiandrosterone (DHEA) is a precursor of about 30-50% of androgens in adult men. DHEA action is partially exerted through its metabolites. Within these, 7-oxo-dehydroepiandrosterone (7-oxo-DHEA) is a biological DHEA metabolite that is not convertible to androgens and has proven to be a promising therapeutic agent. The process o...
Article
Full-text available
Background: Human metabolism is an essential biological process that involves the consumption of different substrates to ensure the nutritional and energetic needs of cells. The disruption of this highly regulated system constitutes the onset of several disorders/dysfunctions such as diabetes mellitus, cardiovascular diseases and hypertension. Ob...
Article
Full-text available
Caffeine, epigallocatechin-3-gallate (EGCG) and L-theanine are major components of tea (Camellia sinensis L.) and main representatives of the classes of methylxanthines, catechins and free amino acids present in this beverage. There are many studies reporting tea’s health benefits, however, it is not clear if those effects are mediated by a single...
Conference Paper
Full-text available
A cafeína é um componente comum do café, chá, bebidas energéticas, produtos que contenham cacau ou chocolate e até mesmo de vários medicamentos. O consumo excessivo de cafeína tem sido associado a diversos problemas de foro reprodutivo. Além disso, foi demonstrado que este composto tem uma ação moduladora no metabolismo celular. Isto levou-nos a co...
Poster
Full-text available
Epigallocatechin-3-gallate (EGCG) is the most abundant catechin in tea (Camellia sinensis (L.) Kuntze, Theaceae). Most of the medicinal properties attributed to tea are suggested to be due to EGCG. Herein, we evaluated the effect of EGCG (5 and 50 μM) on human Sertoli cells (hSCs) metabolism, mitochondrial functionality and oxidative profile. Expos...
Poster
Full-text available
Diabetes mellitus (DM) is a major public health problem and its incidence is dramatically rising. Heart and brain are particularly susceptible to glucose fluctuations and hyperglycaemia-induced oxidative stress. Tea is widely consumed and studied; however, the antidiabetic properties of white tea (WT) remain largely unexplored. In this work, we hyp...
Article
Prediabetes represents a major risk factor for the development of type 2 diabetes mellitus (T2DM). It encompasses some, but not all, T2DM diagnostic criteria. Prediabetes has been recently associated with altered testicular function and increased testicular oxidative stress (OS). Tea is widely consumed and its anti-hyperglycaemic/antioxidant proper...
Poster
Full-text available
Caffeine is a widely consumed substance that has been reported to be a modulator of several cells metabolism. Since the metabolism of Sertoli cells (SCs) is essential for spermatogenesis, we aimed to study the effects of caffeine in human SCs (hSCs) metabolic pathways and hence in male reproductive health. hSCs were cultured in the absence or prese...
Poster
Full-text available
Diabetes mellitus is a major public health problem and its incidence is rising dramatically. Heart and brain, particularly the cerebral cortex, are very susceptible to glucose fluctuations and hyperglycaemia-induced oxidative stress. Tea (Camellia sinensis L.) is widely consumed; however, the antidiabetic properties of white tea remain largely unex...
Poster
Full-text available
Tea is an excellent and inexpensive natural source of polyphenols, especially of catechins and their derivatives. These phytochemicals have strong antioxidant properties and are considered the main responsible for the beneficial health effects attributed to this beverage. Antioxidants are ascribed to reduce cells and biomolecules oxidative stress (...