
Tamas KaucsarSemmelweis University | SOTE · Department of Pathophysiology
Tamas Kaucsar
MD, PhD
About
51
Publications
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Introduction
Publications
Publications (51)
Background:
Ischemia-reperfusion (I/R) is the main cause of acute kidney injury (AKI) in patients. We investigated renal microRNA (miRNA) expression profiles and the time course of changes in selected miRNA expressions after renal I/R to characterize the miRNA network activated during development and recovery from AKI.
Methods and results:
One d...
Micro RNAs (miRNAs) are a recently discovered class of small, non-coding RNAs with the function of post-transcriptional gene expression regulation. MiRNAs may function in networks, forming a complex relationship with diseases. Alterations of specific miRNA levels have significant correlation with diseases of divergent origin, such as diabetic or is...
Elderly patients have increased susceptibility to acute kidney injury (AKI). Long noncoding RNAs (lncRNA) are key regulators of cellular processes, and have been implicated in both aging and AKI. Our aim was to study the effects of aging and ischemia–reperfusion injury (IRI) on the renal expression of lncRNAs. Adult and old (10- and 26–30-month-old...
(1) Background: Ischemia reperfusion (IR) is the leading cause of acute kidney injury (AKI) and results in predisposition to chronic kidney disease. We demonstrated that delayed contralateral nephrectomy (Nx) greatly improved the function of the IR-injured kidney and decelerated fibrosis progression. Our aim was to identify microRNAs (miRNA/miR) in...
Modulated electro-hyperthermia (mEHT) is a selective cancer treatment used in human oncology complementing other therapies. During mEHT, a focused electromagnetic field (EMF) is generated within the tumor inducing cell death by thermal and nonthermal effects. Here we investigated molecular changes elicited by mEHT using multiplex methods in an aggr...
Modulated electro-hyperthermia (mEHT) is a complementary antitumor therapy applying capacitive radiofrequency at 13.56 MHz. Here we tested the efficiency of mEHT treatment in a BALB/c mouse isograft model using the firefly luciferase-transfected triple-negative breast cancer cell line, 4T1. Tumors inoculated orthotopically were treated twice using...
Rats fed a high-fat diet with a single streptozotocin (STZ) injection developed obesity, prediabetes, cardiac hypertrophy and diastolic dysfunction. Here we aimed to explore the renal consequences of prediabetes in the same groups of rats. Male Long-Evans rats were fed normal chow (CON; n = 9) or high-fat diet containing 40% lard and were administe...
(1) Background: Lipopolysaccharide (LPS)-induced systemic inflammation is associated with septic acute kidney injury (AKI). We investigated the time-dependent miRNA expression changes in the kidney caused by LPS. (2) Methods: Male outbred NMRI mice were injected with LPS and sacrificed at 1.5 and 6 h (40 mg/kg i.p., early phase, EP) or at 24 and 48...
(1) Background: Successful treatment of acute kidney injury (AKI)-induced chronic kidney disease (CKD) is unresolved. We aimed to characterize the time-course of changes after contralateral nephrectomy (Nx) in a model of unilateral ischemic AKI-induced CKD with good translational utility. (2) Methods: Severe (30 min) left renal ischemia-reperfusion...
Introduction
Modulated electro‐hyperthermia (mEHT) is a complementary antitumor therapy, based on selective tumor cell killing by a 13.56 MHz radiofrequency induced electric field. The H19 long non coding RNA (lncRNA) is involved in tumor progression and metastasis. It’s overexpression is associated with poor prognosis and therapy resistance in bre...
Background: Pre-treatment with lipopolysaccharide (LPS) protected the kidney against a later lethal ischemia. To reveal the mechanisms of renal cross-tolerance and septic acute kidney injury we investigated the effects of LPS on miRNA expression in the kidney. Methods: Male NMRI mice were injected with 40 and 10 mg/kg LPS ip. and sacrificed at 1.5...
(1) Background: Sepsis-induced acute kidney injury (AKI) is the most common form of acute kidney injury (AKI). We studied the temporal profile of the sepsis-induced renal proteome changes. (2) Methods: Male mice were injected intraperitoneally with bacterial lipopolysaccharide (LPS) or saline (control). Renal proteome was studied by LC-MS/MS (Prote...
Our objective was to develop an electromagnetic tumor therapy device in a consortial cooperation between Semmelweis University and Oncotherm Ltd., to provide data and contribute to the development of the next generation of devices through preclinical, clinical and developmental modules via in vivo, in vitro studies, and patient treatments. Our nume...
Feeding rats with high-fat diet (HFD) with a single streptozotocin (STZ) injection induced obesity, slightly elevated fasting blood glucose and impaired glucose and insulin tolerance, and caused cardiac hypertrophy and mild diastolic dysfunction as published before by Koncsos et al. in 2016. Here we aimed to explore the renal consequences in the sa...
Rats fed a high-fat diet with a single streptozotocin (STZ) injection developed obesity, prediabetes, cardiac hypertrophy and diastolic dysfunction. Here we aimed to explore the renal consequences of prediabetes in the same groups of rats. Male Long-Evans rats were fed normal chow (CON; n = 9) or high-fat diet containing 40% lard and were administe...
Introduction
The effective therapy of triple‐negative breast cancer (TNBC) has not yet been achieved. Modulated electro‐hyperthermia (mEHT) is a novel adjuvant antitumor therapy, based on the highly selective heating of the tumor tissue by a 13.56 MHz radiofrequency current induced electric field.
Aims
Our aim was to investigate the effects of rep...
Aim
We investigated the influence of aging on the lncRNA profile after renal ischemia‐reperfusion (IR) injury in old mice.
Methods
The left renal pedicle of adult (9.4±0.3 months, n=7) and old (28.5±1.2 months, n=8) C57BL/6N mice was clamped for 20 min. The right kidney was left intact. Plasma urea and urine NGAL (uNGAL) were measured prior to and...
Hypoxia-induced long non-coding RNA Malat1 is dispensable for renal ischemia/reperfusion-injury
Renal ischemia-reperfusion (I/R) injury is a major cause of acute kidney injury (AKI). Non-coding RNAs are crucially involved in its pathophysiology. We identified hypoxia-induced long non-coding RNA Malat1 (Metastasis Associated Lung Adenocarcinoma Transcript 1) to be upregulated in renal I/R injury. We here elucidated the functional role of Malat...
Background:
Renal ischemia/reperfusion (I/R) injury represents a major socioeconomic health problem. Non-coding RNA are crucially involved in pathophysiology. Long non-coding RNA Malat1 (Metastasis Associated Lung Adenocarcinoma Transcript 1) was upregulated in renal I/R injury. We elucidated the functional role of Malat1 in vitro and its potential...
Diabetic nephropathy is the main cause of end-stage renal disease. MicroRNAs are powerful regulators of the genome, and global expression profiling revealed miR-21 to be among the most highly regulated microRNAs in kidneys of mice with diabetic nephropathy. In kidney biopsies of diabetic patients, miR-21 correlated with tubulointerstitial injury. I...
Purpose:
Diabetic nephropathy (DN) is the main cause of end-stage renal disease in the Western world. MicroRNAs (miRs) are powerful regulators of the genome. Global expression profiling revealed miR-21 highly regulated in kidneys of mice with diabetic nephropathy. We elucidated miR-21 dependent signaling pathways in vitro and evaluated miR-21 antag...
Background:
Detection of acute kidney injury (AKI) is still a challenge if conventional markers of kidney function are within reference range. We studied the sensitivity and specificity of NGAL as an AKI marker at different degrees of renal ischemia.
Methods:
Male C57BL/6J mice were subjected to 10-, 20- or 30-min unilateral renal ischemia, to c...
Background: Diabetic nephropathy (DN) is the main cause of end-stage renal disease. We therapeutically inhibited microRNA-21 (miR-21) in mice with DN.
Methods:
We used miRNAome analysis, qPCR, in situ PCR, Western Blot, electrophoretic mobility shift assay and bioinformatic algorithms. Furthermore, we fused the 3’UTR of cell division cycle 25a (Cd...
Purpose:
Diabetic nephropathy (DN) is the main cause of end-stage renal disease in the Western world. First, we elucidated novel microRNA (miR) dependent signaling pathways in vitro and in streptozotocin-induced diabetic mice. Furthermore, we evaluated the pharmacological inhibition of miR-21.
Methods:
We performed whole miRNAome expression analys...
Chronic renal fibrosis is the final common pathway of end stage renal disease caused by glomerular or tubular pathologies. Genetic background has a strong influence on the progression of chronic renal fibrosis. We recently found that Rowett black hooded rats were resistant to renal fibrosis. We aimed to investigate the role of sustained inflammatio...
Introduction:
The detrimental effects of diabetes on the vasculature are more relevant than ever before. The aim of our study was to elucidate novel signalling pathways and microRNA expression changes in vitro and in vivo in a mouse model of streptozotocin-induced diabetic nephropathy.
Methods:
Whole genome microRNA microarray analysis and microRN...
Aims: Osteopontin (OPN) is a multifunctional cytokine critically involved in cardiac fibrosis. However, the underlying mechanisms are unresolved. Noncoding RNAs are powerful regulators of gene expression and thus might mediate this process.
Methods and Results: OPN and miR-21 were significantly increased in cardiac biopsies of patients with myocar...
Ischemia-reperfusion (I/R) injury of the kidney is a major cause of AKI. MicroRNAs (miRs) are powerful regulators of various diseases. We investigated the role of apoptosis-associated miR-24 in renal I/R injury. miR-24 was upregulated in the kidney after I/R injury of mice and in patients after kidney transplantation. Cell-sorting experiments revea...
We and others demonstrated previously that preconditioning with endotoxin (LPS) protected from a subsequent lethal LPS challenge or from renal ischemia-reperfusion injury (IRI). LPS is effective in evoking the heat shock response, an ancient and essential cellular defense mechanism, which plays a role in resistance to, and recovery from diseases. H...
Introduction and Aims: Endothelial progenitor cells (EPCs) are bone marrow-derived precursors known to reverse acute kidney injury (AKI) by paracrine
mechanisms including the release of extracellular vesicles (EVs), small particles playing a role in intercellular communication
through the transfer of proteins and RNAs. Activation of the complement...
MicroRNAs (miRNAs) are a recently discovered class of small, non-coding RNAs which do not code proteins. MiRNAs regulate gene expression by inhibiting protein translation from the messenger RNA. MiRNAs may function in networks, forming a complex relationship with diseases. Furthermore, specific miRNAs have significant correlation with diseases of d...
Autism spectrum disorders (ASDs) are a heterogeneous group of clinical conditions characterized by impaired social interaction and communication, and stereotyped behavior that appear in the first 3 years of life. Its etiology is still unknown, but it is most accepted to be multifactorial in origin, with both genetic and environmental factors contri...
Autism spectrum disorders (ASDs), which include the prototypic autistic disorder (AD), Asperger's syndrome (AS) and pervasive developmental disorders not otherwise specified (PDD-NOS), are complex neurodevelopmental conditions of unknown aetiology. The current study investigated the metabolites in the methionine cycle, the transsulphuration pathway...
Autism spectrum disorders (ASD) comprise a complex and heterogeneous group of conditions of unknown aetiology, characterized by significant disturbances in social, communicative and behavioural functioning. Recent studies suggested a possible implication of the high-density lipoprotein associated esterase/lactonase paraoxonase 1 (PON1) in ASD. In t...