Takeshi Hayakawa

Takeshi Hayakawa
Chuo University · Department of Precision Mechanics

PhD

About

110
Publications
7,672
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757
Citations

Publications

Publications (110)
Article
Full-text available
We present a microgripper actuated by a soft microactuator for manipulating a single living cell. Soft actuators have attracted attention in recent years because their compliance which can adapt to soft targets. In this study, we propose a microgripper actuated by soft thermoresponsive hydrogels. The thermoresponsive gel swells in water when the te...
Article
Full-text available
A light-driven gel actuator is a potential candidate for a single-cell manipulation tool because it allows cells to be manipulated while ensuring less damage. Moreover, a large number of actuators can be integrated into a microfluidic chip because no wiring is required. Previously, we proposed a method for cell manipulation using light-driven gel a...
Article
Soft robots have been attracting a lot of attentions in recent years because they have completely different characteristics from conventional robots having rigid structures. We have proposed a soft microrobot using a thermoresponsive gel as a soft actuator. This robot can be driven by being irradiated with light for controlling its temperature. In...
Preprint
Full-text available
A light-driven gel actuator is a potential candidate for a single-cell manipulation tool because it allows cells to be manipulated while ensuring less damage. Moreover, a large number of actuators can be integrated into a microfluidic chip because no wiring is required. Previously, we proposed a method for cell manipulation using light-driven gel a...
Article
Full-text available
Cell rotation is one of the most important techniques for cell manipulation in modern bioscience, as it not only permits cell observation from any arbitrary angle, but also simplifies the procedures for analyzing the mechanical properties of cells, characterizing cell physiology, and performing microsurgery. Numerous approaches have been reported f...
Article
Full-text available
In this study, we evaluated the deformation shape of a balloon-type dielectric elastomer actuator (DEA) that has been prestretched with water pressure. We fabricated the DEA with poly(dimethylsiloxane) (PDMS) as the elastomeric material and carbon grease as the electrode. We derived analytical solutions for the deformation of the DEA based on struc...
Article
Previously, we proposed new light irradiation-based drive method of on-chip gel actuator. In our method, on-chip gel actuators are fixed on a glass substrate with light absorbers. Therefore, deformation of the gel is constricted with fixed part and anisotropically deforms in three dimensions. In this study, we evaluate the three-dimensional shapes...
Article
On-chip actuator is an essential component to realize single cell analysis in microfluidic device. Previously, we proposed a light-driven gel actuator for single cell manipulations. The advantage of this drive method is it enables local heating for integration of large number of actuators on a microfluidic chip. In this study, we present multiple c...
Article
On-chip gel actuator is attracting large attention to realize low-invasive manipulations of various cells. Previously, we proposed a novel drive method of the gel actuator by irradiating light. In addition, we integrated large number of gel actuators in a microfluidic chip and drove these actuators independently for cell manipulation by irradiating...
Article
In this paper, we reported fabricating and driving three-dimensional Dielectric Elastomer Actuator (DEA). DEA is one of the most attractive actuators because of its high responsibility, easy to miniaturize. However, most of DEAs have 2D shape and there is little research toward driving 3D DEA. In this study, we fabricate 3D DEA using dip-mold with...
Article
Previously, we proposed the light drive method of on-chip gel actuators made of PNIPAAm. To use PNIPAAm as an actuator, we need to constrain the degrees of freedom (DOF) of three-dimensional displacement of it. In this study, we add side and top constraints to PNIPAAm and analyze the deformation characteristics under the constraints. We evaluated v...
Article
We have proposed the microgel robot having soft-rigid hybrid structure. We fabricate a microgel robots using two types of material. One is SU-8 that is epoxy resin as a rigid structure. The other is PNIPAAm that is a thermo-responsive gel as a soft actuator. The volume of PNIPAAm changes depending on its temperature. We use the volume change as act...
Article
Attitude control is essential technique for cell surgery or three-dimensional observation of microobjects in field of cell biology and medicine. Previously, we proposed the three-dimensional rotation of microparticles using vibration-induced flow. Vibration-induced flow is generated by the high-speed vibration of a microobject. Therefore, we can ch...
Article
In recent years, cell spheroids have been attracting large attention for their applications in drug efficacy evaluation or tissue engineering for organ and blood vessel regeneration. In our study, we proposed a method to fabricate cell spheroids using vibration-induced flow for easy recovery and mass production. However, due to insufficient design...
Article
In recent years, acoustofluidics, a micromanipulation method by applying acoustic wave to a microfluidic device, has been attracting large attention in medical and biological fields because of their non-invasive and high throughput characteristics. However, its accuracy and stability can be declined by a temperature increase or diffraction in the m...
Article
In this study, we analyzed dynamic characteristics of dielectric elastomer actuator (DEA) with pre-stretch by applying water pressure. The driving system which uses water pressure is able to apply pre-stretch in the out-of-plane direction, so this system can be applied for driving not two-dimensional but three-dimensional DEA. In this system, when...
Article
In this study, we propose a reconfigurable microgel robot which can be assembled and disassembled by light irradiation. Reconfigurable modular robot is attracting large attention because of its scalability, self-healing function, and flexibility. However, few modular robots in microscale have not been proposed yet. We propose a concept of reconfigu...
Article
In this study, we evaluate the vibration-induced flow with high-frequency vibrations. In recent years, acoustic streaming is attracting large attention in the fields of genetics or medicine because acoustic streaming can manipulate micro-nanoparticles with low-damage and high speed. We report micro-nanomanipulation method by using vibration-induced...
Article
In this paper, we made a piezoelectric actuated XY stage which can be driven in high frequency and evaluated a vibration-induced flow when a high frequency vibration was applied to a chip. The vibration-induced flow is a local flow that is induced around microstructures by applying vibration to a microchip with micropillar array patterns. We use pi...
Article
In this study, we propose a method for concentration of nanoparticles based on vibration-induced flow. In recent years, analysis technologies of nanoparticles are attracting large attention in the fields of genetics and medicine. To analyze nanoparticles, concentration techniques of nanoparticles is demanded. Microfluidic chip is one of the powerfu...
Article
In recent years, various micromixers have been reported. However, conventional constant flow-based methods have undesirable dead volume about 10-100 μL in tube connection parts between microfluidic chips and external pumps. Thus, conventional methods are not suitable for mixing of rare samples, such as antibodies, proteins or exosomes. To solve thi...
Article
In this paper, we report a method to fabricate a three-dimensional structures used as a dielectric elastomer actuator (DEA). DEA has been extensively studied because DEA has some advantages, such as fast response and large displacements. However, there are few DEAs having a three-dimensional structure. Therefore, in order to realize three-dimension...
Article
We present the microgripper having soft-rigid hybrid structures without complex feedback systems. Soft materials have sensors and feedback systems in oneself because soft materials can change and fit the shape against stimulations from external world. The proposed microgripper is made of thermoresponsive gel as soft actuators and SU-8 as rigid stru...
Article
Single cell manipulation techniques are needed to realize a single cell analysis. On-chip actuator is a potential candidate for manipulation tool to realize various cell manipulations in a microfluidic chip. Among on-chip actuators, on-chip gel actuator is attracting large attention because it can realize low-invasive cell manipulations. We propose...
Article
On-chip actuator is essential components to realize cell manipulations in a microfluidic device. We proposed novel driving method of hydrogel actuators by using a light irradiation. The hydrogel actuator is made of temperature responsive hydrogel and fabricated on a microfluidic chip with light absorbing micropatterns. Thus, the actuator can be dri...
Article
In this study, we analyze driving characteristics of dielectric elastomer actuator (DEA) with pre-stretch by applying water pressure. To realize driving of three-dimensional soft actuator, we use DEA fabricated by PDMS which has good formability. Stress-strain characteristic of PDMS is linear up to tens of percent deformation. When DEA is driven, p...
Article
On chip manipulation is attracting large attention because of its various applications. Conventionally, on chip flow generation method by using external pump such as syringe pump has two problems, in other words, large system and dead volumes. Thus, we propose a flow control method based on a flow generated by resonance of a microstructure on a chi...
Article
Recently, cell spheroids are attracting large attention in medicine and biology because it can be used for various biomedical applications. In this study, we propose a formation method of cell spheroids on microfluidic chip based on vibration-induced flow. Vibration-induced flow is local flow which is generating around vibrating microobject. Thus,...
Article
In this paper, we propose 3D rotation method of a single microparticle by using 2-DOF vibrations. We have proposed cell manipulation method on a microfluidic chip based on a vibration-induced flow. Additionally, we achieved various two-dimensional cell manipulations in previous studies. However, 3-DOF actuator was required in previous study to real...
Article
Full-text available
The advent of image-activated cell sorting and imaging-based cell picking has advanced our knowledge and exploitation of biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure of the molecular landscape in the cell, which has critical limitations. Here we demon...
Article
On-chip gel actuators are potential candidates for single cell manipulation because they can realize low-invasive manipulation of various cells. We propose an on-chip gel actuator driven by light irradiation. By patterning the gel actuator with light absorber, we can control the temperature of the actuator and drive it. The proposed drive method ca...
Article
Full-text available
We present a driving method for on-chip hydrogel microactuators developed to be used for single-cell manipulations. The hydrogel actuator is composed of a temperature-responsive gel coated on a glass substrate with light-absorbing micropatterning. The actuator is driven by light irradiation, which increases its temperature. The advantage of this dr...
Article
We present a micro-gel robot having soft-rigid hybrid structure. Soft structure work as actuator and rigid structure work as support body. We use SU-8 which is hard photoresist as rigid structure, and bioresist which is thermoresponsive gel as soft structure. The soft structure mixed light absorber and driven by laser irradiation. The micro-gel rob...
Article
Recently, various cell size separation methods are proposed by using microfluidic chips. In this study, we propose cell size separation method based on vibration-induced flow. Vibration-induced flow is induced around microstructure when vibration is applied to a microfluidic chip with microstructure patterns. In addition to that, this flow can be i...
Article
In this paper, we present driving method of on-chip hydrogel microactuator for a single cell manipulation. The hydrogel actuator is made of temperature responsive gel and fabricated on a microfluidic chip with light absorbing micropatterns. Thus, the actuators can be driven by irradiating light on it and increase its temperature. The advantage of t...
Article
We have proposed cell manipulation method on a microfluidic chip based on a vibration-induced flow. We achieved various two-dimensional cell manipulations in previous studies. To realize three-dimensional rotation of cells, 3-DOF actuator was required in previous study. However, vibration-induced flows in vertical (Z) direction is observed when we...
Article
In recent years, various micromixers have been reported. However, conventional constant flow-based methods have undesirable dead volume in the order of 10 μL in tube connection parts between microfluidic chips and external pumps. Thus, conventional methods are not suitable for mixing of rare samples, such as fluorescent reagents or antibodies. To s...
Article
Today, cell spheroid is receiving attention in the field of medical science and drug discovery. In this study, we propose fabrication method of cell spheroids based on vibration-induced flow. The proposed method enables to easily collect fabricated spheroids and mass production of cell spheroids. In this study, we fabricated microfluidic chip with...
Article
This paper presents fabrication and actuation method of soft microrobot having hybrid structure. This hybrid structure enables multi-DOF actuation of the microrobot and complex movement like living microorganisms. In this paper, we show the concept of soft microrobot having soft-rigid hybrid structure and propose a fabrication and an actuation meth...
Article
In this study, we propose fabrication process of multi-layered microfluidic chip using dry film resists (DFR). Microfluidic chip is attracting large attention because of its various applications to chemical or biological fields. Conventionally, microfluidic chips are fabricated by using photolithography process using liquid photoresist. However, it...
Article
Dielectric elastomer actuators (DEAs) are intensively researched because of their fast response, large deformation, and good control characteristics. However, these actuators are conventionally fabricated in non-transparent body and two-dimensional structure. In this study, we perform basic evaluation of transparent DEA toward three-dimensional fab...
Article
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Article
Full-text available
Abstract This paper presents fabrication and actuation methods for a soft microrobot with a hybrid structure composed of soft microactuators and a rigid supporting body. This hybrid structure enables actuation of the microrobot with independent driving of multiple actuators to provide complex movement like that of living microorganisms. We use the...
Article
High-speed isolation of microparticles (e.g., microplastics, heavy metal particles, microbes, cells) from heterogeneous populations is the key element of high-throughput sorting instruments for chemical, biological, industrial and medical applications. Unfortunately, the performance of continuous microparticle isolation or so-called sorting is fund...
Article
Full-text available
Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, imaging flow cytometry is a powerful tool in diverse fields including cancer biology, immunol-ogy, drug discovery, microbiology, and metabolic engineering. It enables measurements and statistical analyses of chemical, structural , and morphological phenot...
Article
Intelligent image-activated cell sorting (iIACS) is a machine-intelligence technology that performs real-time intelligent image-based sorting of single cells with high throughput. iIACS extends beyond the capabilities of fluorescence-activated cell sorting (FACS) from fluorescence intensity profiles of cells to multidimensional images, thereby enab...
Article
Full-text available
Flow cytometry is an indispensable tool in biology for counting and analyzing single cells in large heterogeneous populations. However, it predominantly relies on fluorescent labeling to differentiate cells and, hence, comes with several fundamental drawbacks. Here, we present a high-throughput Raman flow cytometer on a microfluidic chip that chemi...
Article
In this study, we report observation results of vertical flow of vibration-induced flow. Vibration-induced flow is kind of acoustic flow generated around vibrating microobjects. We have previously proposed various cell manipulation method based on a vibration-induced flow. These manipulations utilized two-dimensional flow around the microobjects. H...
Article
In this study, we propose an on-chip cell size filter based on a vibration-induced flow. When circular vibration is applied to a microfluidic chip with micropillar patterns, a highly localized flow is induced around micropillars. We have achieved various cell manipulation using this flow. In this paper, we fabricated micropillar array for cell size...
Article
In this paper, we propose a driving method of on-chip gel actuator by irradiating light. The gel actuators are patterned with light absorbing material and the temperature of the actuators are increased when we irradiate the light. In this study, we used Cr / Au as a light absorber on a glass substrate and PNIPAAm as a gel actuator. We confirmed tha...
Article
In recent years, studies of microrobots have been actively cunduted. However, little microrobots achieved complicated movement like living things. We aim to realize a microrobot capable of complicated movement mimicking living microorganisms. In this study, we propose a microgel robot that can perform peristaltic motion. The proposed robot mimickin...
Article
Full-text available
The steady streaming (SS) phenomenon is gaining increased attention in the microfluidics community, because it can generate net mass flow from zero-mean vibration. We developed numerical simulation and experimental measurement tools to analyze this vibration-induced flow, which has been challenging due to its unsteady nature. The validity of these...
Preprint
Full-text available
The steady streaming (SS) phenomenon is gaining increased attention in the microfluidics community, because it can generate net mass flow from the zero-mean vibration. We developed numerical simulation and experimental measurement tools to analyze this vibration induced flow, which has been challenging due to its unsteady nature. Validity of these...
Article
Label-free particle analysis is a powerful tool in chemical, pharmaceutical, and cosmetic industries as well as in basic sciences, but its throughput is significantly lower than that of fluorescence-based counterparts. Here we present a label-free single-particle analyzer based on broadband dual-comb coherent Raman scattering spectroscopy operating...
Article
Full-text available
The present study was performed to establish a novel ocular surgery simulator for training in peeling of the inner limited membrane (ILM). This simulator included a next-generation artificial ILM with mechanical properties similar to the natural ILM that could be peeled underwater in the same manner as in actual surgery. An artificial eye consistin...
Data
Example of peeling motion of a fabricated ILM in pure water. (MP4)
Data
Example of surgical simulation of ILM peeling with an artificial eyeball mounted on the novel eye surgery simulator. We succeeded in completely simulating the ILM peeling procedure as in actual surgery. (MP4)
Article
Full-text available
We propose an on-chip cell manipulation method for trapping single motile cells in parallel. The proposed method traps large (\(\gtrsim \,50\,\upmu \hbox {m}\)) motile cells in parallel, which is difficult to achieve by conventional cell manipulation methods based on optical, acoustic, electric, or magnetic forces. The trapping method exploits the...
Article
Full-text available
Surgical simulators have recently attracted attention because they enable the evaluation of the surgical skills of medical doctors and the performance of medical devices. However, thermal damage to the human body during surgery is difficult to evaluate using conventional surgical simulators. In this study, we propose a functional surgical model wit...
Article
Full-text available
A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We present a machine-intelligence technology based on a radically different archi...
Chapter
Cell manipulation is one of the most important techniques in recent biotechnology. We have proposed optically driven micro- and nanorobots in order to achieve a variety of minimally-invasive cell manipulations. The robot can be manipulated by using optical tweezers, which enables various cell manipulations, such as transportation, force application...
Article
We propose on-chip cell sorting system using piezo-driven dual membrane pumps. In our method, we integrate a microfluidic chip consisting of glass-Si-glass with piezoelectric actuators. The controllability and the response time of proposed flow control method are evaluated by visualizing and observing the streamline of sample flow. Then euglenas ar...
Article
A well-mimicked human model is useful to improve surgeon's skill of complicated surgeries and to evaluate new medical devices. The human model with artificial organ modules is expected not only to mimic anatomical characteristics and mechanical properties of a human, but also to be integrated with sensors for quantitative evaluation of skills, we c...
Article
This paper reports the stereoscopic model of three-dimensional blood vessel having a temperature Indicator for measurement of temperature variation during surgical training. This model consists of two hydrogel materials to mimic Young's modulus of human artery and rapid fabrication of the model using photo-polymerization. Polyethylene glycol diacry...
Article
We propose an on-chip cell manipulation method for a parallel trapping of single motile cells. The proposed method enables a parallel trapping of large (≳ 50 μm) motile cells, that is difficult to be achieved with conventional cell manipulation methods. We realize the trapping method by using a vibration-induced flow which is induced by applying a...
Article
Although researchers have proposed various methods of on-chip cell sorting, high-throughput sorting of large cells remains hampered by the difficulty of controlling high-speed flow over a wide sorting area. To overcome this problem, we proposed the high-speed local-flow control using dual membrane pumps driven by piezoelectric actuators placed on t...