Taja Železnik Ramuta

Taja Železnik Ramuta
National Institute of Chemistry · Department of Synthetic Biology and Immunology

PhD

About

26
Publications
2,354
Reads
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119
Citations
Citations since 2016
26 Research Items
119 Citations
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201620172018201920202021202201020304050
201620172018201920202021202201020304050
201620172018201920202021202201020304050
Additional affiliations
October 2020 - December 2021
Institute of Cell biology, Faculty of Medicine, University of Ljubljana
Position
  • Researcher
November 2018 - July 2019
Harvard Medical School
Position
  • Fulbright program attendee
October 2016 - September 2020
Institute of Cell biology, Faculty of Medicine, University of Ljubljana
Position
  • PhD Student
Education
October 2013 - September 2016
University of Ljubljana, Biotechnical faculty
Field of study
  • Microbiology
October 2009 - September 2013
University of Ljubljana, Biotechnical faculty
Field of study
  • Microbiology

Publications

Publications (26)
Article
Full-text available
Perinatal derivatives or PnDs refer to tissues, cells and secretomes from perinatal, or birth-associated tissues. In the past 2 decades PnDs have been highly investigated for their multimodal mechanisms of action that have been exploited in various disease settings, including in different cancers and infections. Indeed, there is growing evidence th...
Article
Full-text available
The tumour microenvironment, which is comprised of various cell types and the extracellular matrix, substantially impacts tumour initiation, progression, and metastasis. Mesenchymal stem/stromal cells (MSCs) are one of the key stromal cells in the tumour microenvironment, and their interaction with cancer cells results in the transformation of naïv...
Article
Full-text available
The pathogenesis of neurodegenerative diseases involves the accumulation of misfolded protein aggregates. These deposits are both directly toxic to neurons, invoking loss of cell connectivity and cell death, and recognized by innate sensors that upon activation release neurotoxic cytokines, chemokines, and various reactive species. This neuroinflam...
Article
Full-text available
Urinary tract infections can be severe, sometimes fatal, diseases whose etiological pathogens are predominantly uropathogenic strains of E. coli (UPEC). To investigate the UPEC pathogenesis, several models have already been established with minor or major disadvantages. The aim was to develop a simple, fast, and inexpensive biomimetic in vitro mode...
Article
Full-text available
Background In vitro maturation (IVM) of oocytes is a laboratory method that allows the maturation of immature (GV) oocytes retrieved from patients enrolled in the in vitro fertilization (IVF) programme. However, this method is still sparsely researched and used in clinical practice, leading to suboptimal clinical results. Anti-Müllerian hormone (AM...
Article
Full-text available
Intra-amniotic infection and inflammation (IAI) affect fetal development and are highly associated with preterm labor and premature rupture of membranes, which often lead to adverse neonatal outcomes. Human amniotic membrane (hAM), the inner part of the amnio-chorionic membrane, protects the embryo/fetus from environmental dangers, including microb...
Article
Full-text available
Despite being among the ten most common cancers with high recurrence rates worldwide, there have been no major breakthroughs in the standard treatment options for bladder cancer in recent years. The use of a human amniotic membrane (hAM) to treat cancer is one of the promising ideas that have emerged in recent years. This study aimed to investigate...
Article
Full-text available
The fetal membranes provide a supportive environment for the growing embryo and later fetus. Due to their versatile properties, the use of fetal membranes in tissue engineering and regenerative medicine is increasing in recent years. Moreover, as microbial infections present a crucial complication in various treatments, their antimicrobial properti...
Article
Full-text available
Urinary tract infections (UTIs) represent a serious global health issue, especially due to emerging multidrug-resistant UTI-causing bacteria. Recently, we showed that the human amniotic membrane (hAM) could be a candidate for treatments and prevention of UPEC and Staphylococcus aureus infections. However, its role against multidrug-resistant bacter...
Preprint
Urinary tract infections (UTIs) represent a serious global health issue, especially due to emerging multidrug-resistant UTI-causing bacteria. Recently, we showed that the human amniotic membrane (hAM) could be a candidate for treatments and prevention of UPEC and Staphylococcus aureus infections. However, its role against multidrug-resistant bacter...
Article
Full-text available
Bladder cancer is one of the most common cancers among men in industrialized countries and on the global level incidence and mortality rates are increasing. In spite of progress in surgical treatment and chemotherapy, the prognosis remains poor for patients with muscle-invasive bladder cancer. Therefore, there is a great need for the development of...
Preprint
Bladder cancer is one of the most common cancers among men in industrialized countries and on the global level incidence and mortality rates are increasing. In spite of progress in surgical treatment and chemotherapy, the prognosis remains poor for patients with muscle-invasive bladder cancer. Therefore, there is a great need for the development of...
Patent
The present invention fits in the field of medical or veterinary science, and relates in general to a procedure for the preparation of mammalian amniotic membrane homogenate that can be used as an antimicrobial agent. More specifically, the present invention relates to a procedure for the preparation of an antimicrobial agent characterized in that...
Preprint
Bladder cancer is one of the most common cancers among men in industrialized countries and on the global level incidence and mortality rates are increasing. In spite of progress in surgical treatment and chemotherapy, the prognosis remains poor for patients with muscle-invasive bladder cancer. Therefore, there is a great need for the development of...
Article
Full-text available
Urinary tract infections are among the most common bacterial infections in humans. Moreover, they are highly recurrent and increasingly often resistant to antibiotics. The antimicrobial properties of the amniotic membrane (AM), the innermost layer of fetal membranes, have been briefly reported in the literature, however, the results of published st...
Article
Full-text available
Purpose: Due to the emergence and spread of bacterial strains resistant to antibiotics, the development of new antimicrobials is imperative. The antimicrobial effect of the amniotic membrane (AM) has been explored to a limited extent so far. Materials and methods: We collected 12 biological samples of AM homogenates and tested their antimicrobia...
Preprint
Purpose: Due to the emergence and spread of bacterial strains resistant to antibiotics, the development of new antimicrobials is imperative. The antimicrobial effect of the amniotic membrane (AM), the innermost part of placenta, has been explored to a limited extent so far. Materials and Methods:We collected 12 biological samples of AM homogenates,...
Preprint
Purpose: Due to the emergence and spread of bacterial strains resistant to antibiotics, the development of new antimicrobials is imperative. The antimicrobial effect of the amniotic membrane (AM), the innermost part of placenta, has been explored to a limited extent so far. Materials and Methods:We collected 12 biological samples of AM homogenates,...
Preprint
Purpose: Due to the emergence and spread of bacterial strains resistant to antibiotics, the development of new antimicrobials is imperative. The antimicrobial effect of the amniotic membrane (AM), the innermost part of placenta, has been explored to a limited extent so far. Materials and Methods:We collected 12 biological samples of AM homogenates,...
Preprint
The antimicrobial effect of the amniotic membrane (AM), the innermost part of placenta, has been explored to a limited extent so far. To quantify and better understand the mechanisms behind antibacterial effect of AM, we followed the growth of three common pathogens, uropathogenic Escherichia coli(UPEC), Staphylococcus aureus and Serratia marcescen...
Preprint
The antimicrobial effect of the amniotic membrane (AM), the innermost part of placenta, has been explored to a limited extent so far. To quantify and better understand the mechanisms behind antibacterial effect of AM, we followed the growth of three common pathogens, uropathogenic Escherichia coli(UPEC), Staphylococcus aureus and Serratia marcescen...
Article
Full-text available
Izhodišča: Amnijska membrana (AM) je notranja stran posteljice, ki obdaja in ščiti zarodek. AM je večplastna struktura, ki je sestavljena iz amnijskih epitelijskih celic, amnijskih mezenhimskih stromalnih celic, bazalne lamine in vezivnega tkiva. Iz njene zgradbe izhajajo tudi lastnosti AM, zaradi katerih se že vrsto let uporablja v terapevtske nam...
Article
Full-text available
Izhodišče: Osnovna naloga amnijske membrane je zaščita ploda pred zunanjimi mehanskimi vplivi in izsušitvijo ter zagotavljanje primernega okolja za njegov razvoj. Razumevanje zgradbe in delovanja amnijske membrane je pomembno za njeno klinično uporabo, še posebej v regenerativni medicini. V prispevku opisujemo številne, za regenerativno medicino ze...
Article
Full-text available
Human amniotic membrane (hAM) is the innermost layer of fetal membranes, which surrounds the developing fetus and forms the amniotic cavity. hAM and hAM-derived cells possess many properties that make them suitable for use in regenerative medicine, such as low immunogenicity, promotion of epithelization, anti-inflammatory properties, angiogenic and...

Questions

Question (1)
Question
Hi, I'm having issues with gelatin zymography and I would appreciate your advice. I collected culture media from the T24 cell line (derived from a patient with muscle-invasive bladder cancer) on days 1/7/21 after seeding. The cells were grown on matrigel and the gelatin zymography was performed using a 10% polyacrylamide gel with 0.1% gelatin (I developed the gels for 22 hours or 42 hours). I detected pro-MMP2 in all samples and the size of the bands increased from day 1 to day 21. However, I never detected the active form of MMP2 in any of the samples I tested (I use the recombinant active MMP-2 as a standard and therefore, I'm sure I only get the pro-MMP2 and not the active MMP2). Moreover, using fluorescence microscopy I confirmed that the cells did cross the matrigel.
Best regards,
Taja

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